Article
Food Science & Technology
Zhipeng Gao, Weiming Zhong, Ting Liu, Tianyu Zhao, Jiajing Guo
Summary: In this study, the response of Listeria monocytogenes exposed to linalool was explored using iTRAQ-based quantitative proteomics analysis to uncover the mode of action and drug targets of linalool against LM. A total of 445 differentially expressed proteins were identified, with 31 significantly enriched gene ontology functional categories and 60 significantly enriched biological pathways. The analysis suggested that cell membranes, cell walls, nucleoids, and ribosomes might be the targets of linalool against LM.
Article
Biochemical Research Methods
Hanbing Huang, Jialin He, Xiangyu Gao, Jun Lei, Yuxi Zhang, Yan Wang, Xueqiang Liu, Jianxiong Hao
Summary: This study investigated the deactivation mechanisms of Listeria monocytogenes by acidic electrolyzed water treatment. Proteomic analysis showed that this treatment affected protein transcription and extension, RNA processing and synthesis, gene regulation, sugar and amino acid transport and metabolism, signal transduction, and ATP binding. The findings contribute to the understanding of the development of electrolyzed water as a method for removing biofilms and provide theoretical support for addressing microbial contamination in food processing.
JOURNAL OF PROTEOMICS
(2023)
Article
Biotechnology & Applied Microbiology
Bingren Tian, Dan Xu, Wanrong Li, Jie Wang, Jianhua Cheng, Yumei Liu
Summary: This study aimed to investigate the regulatory and shaping effects of HBA/HP-beta-CD on the proteome of L. monocytogenes. The results showed that HBA/HP-beta-CD exhibited significant antibacterial activity against L. monocytogenes, and the changes in the proteome were mainly associated with carbohydrate metabolism, protein synthesis, cell wall composition, and cell division.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Constanza Melian, Patricia Castellano, Franco Segli, Lucia M. Mendoza, Graciela Margarita Vignolo
Summary: The study showed that treating Listeria monocytogenes with sublethal dose of lactocin AL705 can reduce biofilm formation and induce adaptation mechanisms in the treated cells. This approach targets specific proteins to control L. monocytogenes biofilm, providing valuable data for future research.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Xiaowei Fang, Yu Zhang, Qian Guo, Mei Yuan, Xiongyan Liang, Jing Liu, Shouguo Fang, Yuying Yang, Chun Fang
Summary: This study demonstrates that the deletion of gadT2/gadD2 significantly reduces the survival rate of Listeria monocytogenes under different acidic conditions. Additionally, the knockout of the transcription factor gadR4 increases the acid stress tolerance of L. monocytogenes.
Article
Microbiology
Zexuan Song, Shunshi Ji, Yan Wang, Lijuan Luo, Yiqian Wang, Pan Mao, Lingling Li, Huaying Jiang, Changyun Ye
Summary: This study analyzed the whole-genome sequences of 207 ST9 isolates from different sources, geographical locations, and isolated years. The analysis revealed different features in ST9 isolates, such as the presence of plasmids, prophages insertion, premature stop codon mutations in inlA, and the presence of arsenic resistance genes and environment-resistance genes. These findings can help in developing effective strategies to control Listeria monocytogenes in the food chain.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biology
Yi-An Chen, Guan-Wen Chen, Hao-Hsiang Ku, Tsui-Chin Huang, Hsin-Yi Chang, Cheng- Wei, Yung-Hsiang Tsai, Tai-Yuan Chen
Summary: This study investigated the inactivation mechanisms of Listeria monocytogenes under high-pressure processing (HPP) through label-free quantitative proteomic analysis. The results revealed that HPP can promote translation initiation but impairs protein synthesis due to a shortage of proteins responsible for elongation, termination, and recycling. These findings provide theoretical support for using HPP to combat Listeriosis illness and improve the safety of ready-to-eat foods.
Article
Biotechnology & Applied Microbiology
Federica Palma, Nicolas Radomski, Alizee Guerin, Yann Sevellec, Benjamin Felix, Arnaud Bridier, Christophe Soumet, Sophie Roussel, Laurent Guillier
Summary: The study found that mobile elements were associated with biocide tolerance in Lm strains. These elements, including prophage-related loci, the Tn6188_qacH transposon, and pLMST6_emrC plasmid, were widespread in food strains and linked to tolerance to benzalkonium-chloride and didecyldimethylammonium-chloride. Additionally, genes encoding for cell-surface proteins were associated with tolerance to benzalkonium-chloride and polymeric biguanide.
Article
Biotechnology & Applied Microbiology
Federica Palma, Nicolas Radomski, Alizee Guerin, Yann Sevellec, Benjamin Felix, Arnaud Bridier, Christophe Soumet, Sophie Roussel, Laurent Guillier
Summary: Different genetic markers were found to play a role in Lm adaptation to biocides, including mobile elements such as prophage-related loci, transposons, and plasmids. These markers could help predict and monitor the spread of biocide-tolerant Lm genotypes across different ecological niches.
Article
Biochemistry & Molecular Biology
Maria Guadalupe Avila-Novoa, Berenice Gonzalez-Torres, Jean Pierre Gonzalez-Gomez, Pedro Javier Guerrero-Medina, Liliana Martinez-Chavez, Nanci Edid Martinez-Gonzales, Cristobal Chaidez, Melesio Gutierrez-Lomeli
Summary: In this study, the diversity of virulence factors associated with the mechanisms of pathogenicity, persistence, and formation of biofilm in L. monocytogenes were investigated using whole-genome sequencing. The findings contribute to the understanding of the genomic diversity and epidemiology of L. monocytogenes, and provide valuable insights for establishing control measures to reduce its impact on the environment and consumers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Samantha Bolten, Anna Sophia Harrand, Jordan Skeens, Martin Wiedmann
Summary: This study evaluated the tolerance of produce-associated L. monocytogenes and other Listeria spp. to benzalkonium chloride (BC) and found that they can acquire heritable adaptations to low concentrations of BC. However, these adapted isolates remain sensitive to the concentrations of BC used for food contact surface sanitation.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Zuzana Kubicova, Sophie Roussel, Benjamin Felix, Lenka Cabanova
Summary: Over the past 11 years, the Slovak National Reference Laboratory collected 988 Listeria monocytogenes isolates in Slovakia from various sources, including food sectors, animals, and human cases. The serotyping revealed that serotype 1/2a was the most prevalent, especially in the meat sector. The analysis of CCs demonstrated that CC11-ST451 was the most prevalent CC, particularly in food and animal isolates.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hsin-Yi Chang, Chin-Ying Gui, Tsui-Chin Huang, Yen-Con Hung, Tai-Yuan Chen
Summary: This study investigates the impact of slightly acidic electrolyzed water (SAEW) on Listeria monocytogenes and uncovers the molecular mechanisms leading to the viable but nonculturable (VBNC) state. Proteomic techniques were used to identify differentially expressed proteins and functional enrichment analysis revealed the affected pathways. The study also highlights the role of protein chlorination and its potential consequences. These findings have significant implications for food safety and provide potential strategies for controlling L. monocytogenes in the VBNC state.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Damayanti Chakravarty, Gyan Sahukhal, Mark Arick II, Morgan L. Davis, Janet R. Donaldson
Summary: This study analyzed the differences in transcript levels produced by Listeria monocytogenes under conditions mimicking the gastrointestinal tract, revealing that anaerobic environments have an impact on gene transcription, particularly under acidic conditions. These findings provide comprehensive information on how L. monocytogenes responds to the gastrointestinal tract.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Food Science & Technology
Rebecca Bland, Stephanie R. B. Brown, Joy Waite-Cusic, Jovana Kovacevic
Summary: The development of antibiotic resistance and reduced susceptibility to sanitizers pose significant challenges to public health. Understanding the effectiveness of sanitizers in combating foodborne pathogens and the factors that impact their efficacy is crucial for the food industry. However, inconsistencies in methodology and definitions regarding sanitizer resistance, tolerance, and susceptibility create confusion in research findings.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2022)
Article
Biotechnology & Applied Microbiology
Efstathios S. Giotis, Alistair Scott, Lisa Rothwell, Tuanjun Hu, Richard Talbot, Daniel Todd, David W. Burt, Elizabeth J. Glass, Pete Kaiser
JOURNAL OF GENERAL VIROLOGY
(2018)
Article
Veterinary Sciences
Efstathios S. Giotis, Michael A. Skinner
Article
Genetics & Heredity
Efstathios S. Giotis, Guillaume Montillet, Bertrand Pain, Michael A. Skinner
Article
Energy & Fuels
Davidraj Johnravindar, Namasivayam Elangovan, Nellaiappan Olaganathan Gopal, Arunachalam Muthaiyan, Qiang Fei
Summary: Tapioca starch factories generate a large amount of wastewater and solid waste, with high levels of starchy lignocellulosic materials like cassava waste residue. Clostridium sp. AS3 strain showed promising biobutanol production capacity from CWR under optimized conditions, demonstrating potential for continuous production.
Article
Microbiology
Efstathios S. Giotis, George Carnell, Erik F. Young, Saleena Ghanny, Patricia Soteropoulos, Lin-Fa Wang, Wendy S. Barclay, Michael A. Skinner, Nigel Temperton
NATURE MICROBIOLOGY
(2019)
Article
Environmental Sciences
Thangavel Mohankumar, Haralur Shankaraiah Lalithamba, Krishnan Manigandan, Arunachalam Muthaiyan, Namasivayam Elangovan
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2020)
Review
Immunology
Efstathios S. Giotis
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2020)
Article
Immunology
Katherine L. Dulwich, Amin Asfor, Alice Gray, Efstathios S. Giotis, Michael A. Skinner, Andrew J. Broadbent
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Efstathios S. Giotis, Stephen M. Laidlaw, Susanna R. Bidgood, David Albrecht, Jemima J. Burden, Rebecca C. Robey, Jason Mercer, Michael A. Skinner
Article
Virology
Amin S. Asfor, Salik Nazki, Vishwanatha R. A. P. Reddy, Elle Campbell, Katherine L. Dulwich, Efstathios S. Giotis, Michael A. Skinner, Andrew J. Broadbent
Summary: This study inoculated a very virulent strain of IBDV into four different chicken lines, with the 15I line experiencing milder disease. Analysis revealed that the severity of the disease was associated with up-regulation of inflammatory pathways, with birds from the W line showing more rapid pro-inflammatory responses compared to the 15I line.
Article
Multidisciplinary Sciences
D. A. Leach, A. Mohr, E. S. Giotis, E. Cil, A. M. Isac, L. L. Yates, W. S. Barclay, R. M. Zwacka, C. L. Bevan, G. N. Brooke
Summary: Antiandrogen treatment reduces TMPRSS2 expression in the lung, decreasing SARS-CoV-2 viral entry and infection, supporting the potential of antiandrogens as a therapeutic approach for COVID-19.
NATURE COMMUNICATIONS
(2021)
Article
Virology
Ziyun Zhang, Rebecca Penn, Wendy S. Barclay, Efstathios S. Giotis
Summary: In this study, the researchers investigated the interaction between SARS-CoV-2 and human macrophages. They found that macrophages were not susceptible to SARS-CoV-2 infection, but limited replication of influenza viruses was observed. The cytokine response in SARS-CoV-2-exposed macrophages was moderate, with a notable absence of IFN-beta induction, while the influenza viruses strongly induced this response. Pre-treatment of macrophages with IFN-alpha enhanced proinflammatory cytokine expression after virus exposure.
Review
Virology
Efstathios S. Giotis, Emine Cil, Greg N. Brooke
Summary: COVID-19 has caused over 6.5 million deaths worldwide. The emergence of SARS-CoV-2 variants with increased transmissibility and/or virulence, as well as immune and vaccine escape capabilities, highlights the urgent need for effective antiviral treatments. Men are more susceptible to the virus and have higher rates of severe disease, hospitalization, and mortality, which is associated with sex steroid hormones, particularly androgens. Modulating androgen signaling may be a promising strategy to prevent viral infection and antiandrogens could be used early in COVID-19 to mitigate the disease.
Correction
Multidisciplinary Sciences
Peter Jianrui Liu, James M. Harris, Emanuele Marchi, Valentina D'Arienzo, Thomas Michler, Peter A. C. Wing, Andrea Magri, Ana Maria Ortega-Prieto, Maarten van de Klundert, Jochen Wettengel, David Durantel, Marcus Dorner, Paul Klenerman, Ulrike Protzer, Efstathios S. Giotis, Jane A. McKeating
SCIENTIFIC REPORTS
(2020)