Article
Polymer Science
Haoyang Ren, Bin Gao, Mengfan Wang
Summary: This study investigates the formation process and structure characteristics of the polyacrylonitrile (PAN)-iodine complex using infrared and Raman spectroscopy. It is found that the PAN-iodine complex can form through intermolecular interactions between PAN chains and I-5(-) ions in iodine aqueous solution with a concentration higher than 0.1 mol/L. The infrared spectra of PAN show significant shifts in the nu(CN) and delta(CH2) modes when complex formation occurs in 1 mol/L iodine solution. Moreover, PAN-iodine complexes formed in iodine solutions with different counter ions (K+, Na+, Li+) may have different structures based on their infrared spectra. Interestingly, the content of planar zigzag conformation in the atactic PAN chain increases during complex formation.
Article
Nanoscience & Nanotechnology
Danni Chen, Heng Li, Bin Yu, Junle Qu
Summary: This study proposes a method for researching dynamic events in living cells by simultaneously monitoring spatial positions and changes in local environment related to fluorescence lifetime. The feasibility of the method is verified through experiments, and intracellular endocytosis in a living cell is successfully observed.
Article
Optics
Shuhao Qian, Jia Meng, Wenjie Liu, Chuncheng Wang, Rushan Jiang, Lu Yang, Xu Liu, Cuifang Kuang, Zhihua Ding, Zhiyi Liu
Summary: This study proposes a multi-parametric, quantitative method to assess endoplasmic reticulum (ER) reorganization. By analyzing time-dependent images of co-labeled ER and microtubule (MT), it is found that morphological features are sensitive to ER reshaping and can be potential biomarkers for ER formation. A new mechanism of MT-associated ER formation is identified, called hooking, which is different from the other three known mechanisms.
Article
Biochemistry & Molecular Biology
Genta Kamiya, Nobuo Kitada, Tadaomi Furuta, Takashi Hirano, Shojiro A. Maki, Sung-Bae Kim
Summary: A unique combinatorial bioluminescence imaging system was developed to determine molecular events in mammalian cells. This system utilizes various colors and BL intensity patterns by using reporter luciferases and novel coelenterazine analogues. The synthesized substrates showed unique specificity and BL intensity patterns with marine luciferases, enabling the identification of different reporters and ligand-specific fold intensities in mammalian cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Ayush Somani, Arif Ahmed Sekh, Ida S. Opstad, Asa Birna Birgisdottir, Truls Myrmel, Balpreet Singh Ahluwalia, Alexander Horsch, Krishna Agarwal, Dilip K. Prasad
Summary: This paper presents a novel method to visualize mitochondria in living cells without fluorescent markers. The authors proposed a physics-guided deep learning approach to obtain virtually labeled micrographs of mitochondria from bright-field images. The results showed that the virtual labeling approach significantly outperformed state-of-the-art techniques in segmenting and tracking individual mitochondria.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Environmental Sciences
Cheng-Yen Chiang, Kun-Shan Chen, Ying Yang, Yang Zhang, Tong Zhang
Summary: This study presents a GPU-based computation for simulating SAR images of complex targets, involving multiple scattering fields and antenna pattern tracking. By optimizing computation parallelization and CUDA kernel functions, the computational efficiency is significantly improved by 224 times, with the validation of the proposed simulation algorithm and demonstration of high fidelity in simulated images.
Article
Chemistry, Analytical
Yi-Fan Tian, Yu-Peng Zhang, Qiu-Mei Wu, Dai-Wen Pang, Shu-Lin Liu, Zhi-Gang Wang
Summary: Translation is a critical cellular activity regulated by viruses, and studying its kinetics and subcellular localization requires real-time visualization techniques. This study presents a new method using the CRISPR/dCas13 system and specific complementary guide RNAs to dynamically image translation without altering the coding regions. This tool provides promising opportunities for further investigating translation mechanisms.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yi-Fan Tian, Yu-Peng Zhang, Qiu-Mei Wu, Dai-Wen Pang, Shu-Lin Liu, Zhi-Gang Wang
Summary: This article introduces a method for real-time dynamic translation imaging using the CRISPR/dCas13 system. The method labels the unmodified coding regions of target mRNA, enabling real-time visualization of the translation process, and is promising for further investigations into translation mechanisms.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Xue Wu, Xiaoxiu Wang, Ying Li, Fanpeng Kong, Kehua Xu, Lu Li, Bo Tang
Summary: Researchers developed four easily prepared LDs-specific probes based on noncharged merocyanines, among which LD-1 showed high sensitivity and LDs-targeting ability, successfully applied for cancer diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Cristina Chirizzi, Carlo Morasso, Alessandro Aldo Caldarone, Matteo Tommasini, Fabio Corsi, Linda Chaabane, Renzo Vanna, Francesca Baldelli Bombelli, Pierangelo Metrangolo
Summary: Molecular imaging techniques play a vital role in studying biological processes and detecting disease biomarkers. Multimodal diagnostic probes are essential for imaging across various scales, while fluorinated (F-19) probes show promise for in vivo cell tracking. PERFECTA, a bioorthogonal F-19 probe, combines excellent features for macroscopic F-19-MRI and microscopic Raman imaging at tissue and cellular levels.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Engineering, Biomedical
Yizhou Jiang, Xuandi Hou, Xinyi Zhao, Jianing Jing, Lei Sun
Summary: The field of NK cell-based immunotherapy for cancer treatment has seen significant growth in recent years. In this study, a strategy for tracking NK cells in cancer immunotherapy using ultrasound imaging modality was described. Nanosized ultrasound contrast agents, gas vesicles, were used to label NK cells, allowing for noninvasive tracking without impacting cellular viability or migration. The strategy successfully monitored the trafficking of systemically infused NK cells to a subcutaneous tumor model, with enhanced trafficking observed in co-treatment with interleukin-2. This ultrasound-based tracking strategy provides valuable information for evaluating and optimizing NK cell-based immunotherapy.
ACTA BIOMATERIALIA
(2023)
Article
Instruments & Instrumentation
Margriet van der Zee, Claudette de Vries, Marc Masa, Marta Morales, Marta Rayo, Ingrid Hegger
Summary: The ability to track therapeutic cells is important for regulators and developers of cell therapy. The Horizon2020 project nTRACK aimed to develop a nano-imaging agent for tracking therapeutic cells during the development of cell therapy. The regulatory classification of the nTRACK agent posed challenges, and standard test methods for demonstrating quality and safety were not always suitable for nanomaterials. Regulatory agility is needed to prevent delays in medical innovations, and experience is expected to improve regulatory guidance. This article outlines lessons learned and offers recommendations for regulators and developers of similar products.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Chemistry, Analytical
Dongxia Chen, Yaying Lin, Ao Li, Xiangjie Luo, Chaoyong Yang, Jinhao Gao, Hongyu Lin
Summary: This study presents a strategy called bio-orthogonal metabolic fluorine labeling for selective cellular F-19 labeling, enabling high specific imaging of tumor cells in vivo. The strategy involves the display of azido groups on the cell surface and subsequent bio-orthogonal ligation to incorporate F-19-containing moieties on the cancer cell surface. The feasibility of this method was validated on the cellular level and further demonstrated for in vivo deep-tissue visualization of cancer cells using F-19 MRI.
ANALYTICAL CHEMISTRY
(2022)
Article
Geochemistry & Geophysics
Jianqiu Wang, Kang Liu, Hongyan Liu, Kaicheng Cao, Yongqiang Cheng, Hongqiang Wang
Summary: This article presents a solution to achieve 3-D electromagnetic (EM) vortex imaging in radar imaging technology by effectively utilizing the relative motion between radar and target in the line-of-sight direction, resulting in superior imaging performance.
IEEE TRANSACTIONS ON GEOSCIENCE AND REMOTE SENSING
(2022)
Article
Multidisciplinary Sciences
Kyung Oh Jung, Ashok Joseph Theruvath, Hossein Nejadnik, Anna Liu, Lei Xing, Todd Sulchek, Heike E. Daldrup-Link, Guillem Pratx
Summary: Regenerative medicine uses patient own stem cells to regenerate damaged tissues. This study presents a rapid, effective, and straightforward method for cell labeling using mechanoporation. The labeled cells can be monitored post-implantation using PET imaging.
SCIENTIFIC REPORTS
(2022)