期刊
BMC MEDICAL GENOMICS
卷 5, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1755-8794-5-18
关键词
MicroRNA; Cancer stem cell; Ovarian cancer; CD133; OVCAR3; Chemoresistance
资金
- Yonsei University Research Fund [6-2010-0006]
- Faculty Research Grant of Yonsei University College of Medicine [6-2009-0127]
- National Research Foundation of Korea
- Korean Government [2010-0004672, 2011-0010800]
- Korea Healthcare technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea [A084120]
- National Research Foundation of Korea [2011-0010800, 2010-0004672] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Background: Cancer stem cells (CSCs) are thought to be a source of tumor recurrence due to their stem cell-like properties. MicroRNAs (miRNAs) regulate both normal stem cells and CSCs, and dysregulation of miRNAs has an important role in tumorigenesis. Cluster of differentiation (CD) 133(+) and spheroid formation have been reported to be one of the main features of ovarian CSCs. Therefore, we determined the miRNA expression profile of a CD133(+) spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line. Methods: Initially, we confirmed the enrichment of the OVCAR3 CD133 subpopulation by evaluating in vitro anchorage-independent growth. After obtaining a subpopulation of CD133(+) OVCAR3 cells with > 98% purity via cell sorting, miRNA microarray and real-time reverse transcription-polymerase chain reaction (RT-PCR) were performed to evaluate its miRNA profile. Results: We found 37 differentially expressed miRNAs in the CD133(+) spheroid-forming subpopulation of OVCAR3 cells, 34 of which were significantly up-regulated, including miR-205, miR-146a, miR-200a, miR-200b, and miR-3, and 3 of which were significantly down-regulated, including miR-1202 and miR-1181. Conclusions: Our results indicate that dysregulation of miRNA may play a role in the stem cell-like properties of ovarian CSCs.
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