Article
Microbiology
Weishuai Zhai, Yingxin Tian, Mi Lu, Muchen Zhang, Huangwei Song, Yulin Fu, Tengfei Ma, Chengtao Sun, Li Bai, Yang Wang, Dejun Liu, Ying Zhang
Summary: This study identified a clinical Klebsiella pneumoniae isolate harboring the tet(X4) gene on a non-self-transferable plasmid. The plasmid could be cotransferred with another conjugative plasmid to Escherichia coli, suggesting an increasing risk of spreading tet(X4) among important pathogens.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Alessandra Carattoli, Gabriele Arcari, Giulia Bibbolino, Federica Sacco, Dario Tomolillo, Federica Maria Di Lella, Maria Trancassini, Luigi Faino, Mario Venditti, Guido Antonelli, Giammarco Raponi
Summary: A study conducted in a university hospital in Rome, Italy identified multiple CZA-resistant KPC-3 protein variants, including novel variants that had not been reported before. These variants showed different sensitivities and activities to antibiotics, possibly due to mutations caused by amino acid substitutions, insertions, and deletions.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Immunology
Lilan Sun, Lang Sun, Xue Li, Xinxin Hu, Xiukun Wang, Tongying Nie, Youwen Zhang, Xuefu You
Summary: ML-7 hydrochloride (ML-7) has been identified as a potential novel tigecycline adjuvant to combat tigecycline-resistant Klebsiella pneumoniae. ML-7 exhibits synergistic bactericidal activities with tigecycline, inhibits efflux pump function, disrupts proton motive force, reduces intracellular ATP levels, and leads to accumulation of ROS. Furthermore, transcriptomic analysis reveals the enrichment of ML-7-related genes in ABC transporters.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
I-Ren Chen, Shih-Neng Lin, Xin-Ni Wu, Sheng-Hua Chou, Fu-Der Wang, Yi-Tsung Lin
Summary: Klebsiella pneumoniae is a common pathogen causing nosocomial pneumonia and community-acquired pneumonia in Asia. This study found that K. pneumoniae bacteremic pneumonia has a high mortality rate, with nosocomial pneumonia having a higher mortality rate compared to community-acquired pneumonia. Factors such as not receiving appropriate definitive therapy, disease severity, and host factors contribute to the 28-day mortality.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Si-Ho Kim, Cheon-Hoo Jeon, Hyoung-Tae Kim, Yu Mi Wi
Summary: This study investigated the clinical characteristics of patients with hypermucoviscous Klebsiella pneumoniae (hmvKp) bacteremia from non-hepatobiliary tract infection. The results showed that hmvKp was associated with less frequent drug resistance and metastatic-purulent presentation, but not with clinical outcomes.
Article
Medicine, General & Internal
Yanzhu Chen, Yi Chen
Summary: Hypervirulent Klebsiella pneumoniae (hvKP) is a pathogen with higher virulence than classical strains, posing a significant threat to public health in China. The country faces the urgent task of identifying hvKP and providing optimal treatment.
JOURNAL OF TRANSLATIONAL INTERNAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Xiaojuan Wang, Xiaoling Xu, Shaojun Zhang, Na Chen, Yunfeng Sun, Kuifen Ma, Dongsheng Hong, Lu Li, Yongzhong Du, Xiaoyang Lu, Saiping Jiang
Summary: The article describes the design of a nanorod drug delivery system that overcomes Klebsiella pneumonia resistance to tigecycline by increasing drug accumulation, inhibiting efflux pumps, and targeting bacteria, thus enhancing the drug's antibacterial activity.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Maytham Hussein, Zetao Sun, Jane Hawkey, Rafah Allobawi, Louise M. Judd, Vincenzo Carbone, Rajnikant Sharma, Varsha Thombare, Mark Baker, Gauri G. Rao, Jian Li, Kathryn E. Holt, Tony Velkov
Summary: This study investigated the mechanism of nitrofurantoin action and resistance in Klebsiella pneumoniae. The researchers found that highly resistant mutants showed modified expression of genes related to membrane transport and nitroreductase activity. Metabolomic analysis revealed global perturbations in response to nitrofurantoin exposure. The study also highlighted the key role of efflux pump regulator oqxR in nitrofurantoin resistance.
Article
Immunology
Ying Qu, Wenji Wang, Qinhong Lu, Jihai Qiu, Dongguo Wang, Liman Ma
Summary: This study aimed to investigate the genetic features of the tmexCD1-toprJ1 gene cassette in Klebsiella pneumoniae strain F4_plasmid pA. The study revealed that strain F4 was resistant to antibiotics, especially tigecycline, due to the presence of the oqxAB gene and tmexCD1-toprJ1 gene cassette. Plasmid transfer assays confirmed the presence of tmexCD1-toprJ1 and bla(DHA-1) genes in plasmids pA and pB. Furthermore, plasmid pA exhibited genetic intermixing with TnAs1, Tn5393, TnAs3, and In641 genes, while plasmid pB showed structural overlap with TnAs3 and In641 genes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
M. Ortiz-Padilla, I Portillo-Calderon, B. de Gregorio-Iaria, J. Blazquez, J. Rodriguez-Bano, A. Pascual, J. M. Rodriguez-Martinez, F. Docobo-Perez
Summary: This study aimed to characterize the role of specific genes in fosfomycin resistance in Klebsiella pneumoniae and assess the potential synergistic effect of combining fosfomycin with sodium phosphonoformate. The results showed that inactivation of the fosA gene led to decreased fosfomycin resistance, while the addition of PPF had limited effects and showed decreased activity in the presence of fosfomycin-resistant mutations.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Wanni Cai, Jianbang Kang, Yanbin Ma, Donghong Yin, Yan Song, Yujie Liu, Jinju Duan
Summary: This study provides detailed insight into Carbapenem Resistant Klebsiella pneumoniae (CRKP) in Shanxi. The clinical characteristics of CRKP vary between different regions in Shanxi. The dominant sequence type (ST) in Shanxi is ST11, but its proportion has decreased over time. NDM is the most common carbapenemase in Shanxi. Furthermore, the distribution of STs, carbapenemases, serotypes, and virulence genes varies across different regions in Shanxi. The combination of tigecycline with carbapenems and aztreonam shows excellent synergistic effect on CRKP in vitro.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Infectious Diseases
Bhuvan Yadav, Srujana Mohanty, Bijayini Behera
Summary: This study aimed to investigate the occurrence of hypervirulent Klebsiella pneumoniae (hvKP) isolates from different clinical specimens in a tertiary care hospital in eastern India, and to study the distribution of virulence factors, capsular serotypes, and antibiogram profile. The distribution of carbapenemase-encoding genes in convergent (hvKP and carbapenem-resistant) isolates was also examined.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Infectious Diseases
Binzhi Dan, Heping Dai, Dangui Zhou, Hongfang Tong, Mei Zhu
Summary: The objective of this study was to analyze the epidemiology of hypervirulent Klebsiella pneumoniae (K. pneumoniae) and explore its drug resistance, ESBLs gene, virulence factor, capsular serotype, and biofilm formation. K. pneumoniae strains were isolated from clinical samples and identified using VITEK2 Compact. Antibiotic susceptibility was determined using microbroth dilution test, while ESBLs production and carbapenemase production were detected using specific methods. PCR was used to detect ESBLs genes, virulence factors, and capsular serotypes. Crystal violet staining was used to assess biofilm formation. The study found that ESBLs genes blaTEM, blaSHV, and blaCTX-M were detected in the K. pneumoniae strains, and multiple ESBLs genes were present in most strains. The virulence genes fimH, ybtS, entB, rmpA, aerobactin, allS, and kfu were also commonly found. It was observed that a significant correlation existed between ESBLs-producing strains and biofilm formation. In conclusion, classical K. pneumoniae has a propensity to acquire antibiotic resistance compared to hypervirulent strains. Moreover, genes encoding rmpA, K1 or K2, and kfu were highly associated with hypervirulent K. pneumoniae.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Public, Environmental & Occupational Health
Tingting Le, Ling Wang, Chaoying Zeng, Leiwen Fu, Zhihua Liu, Jing Hu
Summary: Nosocomial and healthcare-associated K. pneumoniae infections displayed similar clinical features and antimicrobial resistance, differing from community-acquired infections. CRKP and ESBL-producing strains were mainly found in HCA and nosocomial groups, showing clonal diversity. Cross transmission of CRKP existed among HCA and nosocomial patients, suggesting the need for consistent empirical therapy and resistance surveillance for these infections.
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2021)
Article
Immunology
Victoria Ballen, Yaiza Gabasa, Carlos Ratia, Raquel Ortega, Marc Tejero, Sara Soto
Summary: Uropathogenic Klebsiella pneumoniae strains demonstrate higher antimicrobial resistance, ESBL production, and biofilm-forming ability compared to strains from other clinical sources.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)