Article
Biotechnology & Applied Microbiology
Carolina P. Moraleda, Diego Robledo, Alejandro P. Gutierrez, Jorge del-Pozo, Jose M. Yanez, Ross D. Houston
Summary: The resistance to SRS is a polygenic trait, and this study has identified relevant genes and pathways that are likely to play a role in mediating genetic resistance through genome-wide association analysis and RNA-sequencing.
Article
Biotechnology & Applied Microbiology
O. Gervais, A. Barria, A. Papadopoulou, R. L. Gratacap, B. Hillestad, A. E. Tinch, S. A. M. Martin, D. Robledo, R. D. Houston
Summary: The study found that the heritability of ISAV resistance is moderate with a polygenic architecture, but a significant quantitative trait locus was detected on chromosome 13. A mild up-regulation of the interferon pathway characterizes the response to the virus in heart samples from this population of Atlantic salmon, and candidate genes showing differential expression between samples with high and low breeding values for resistance were identified.
Article
Biotechnology & Applied Microbiology
Ophelie Gervais, Carolina Penaloza, Remi Gratacap, Athina Papadopoulou, Mariana Beltran, Neil C. Henderson, Ross D. Houston, Musa A. Hassan, Diego Robledo
Summary: In this study, single-cell RNA sequencing was used to provide a high-dimensional insight into the transcriptional landscape of Atlantic salmon cells during early Infectious Salmon Anaemia Virus (ISAV) infection. The results showed a clear anti-viral response in infected cells, characterized by the upregulation of IFNA2 or IRF2. Additionally, uninfected bystander cells exhibited transcriptional differences, suggesting paracrine signaling from infected cells. This study identified potential key genes involved in the host-virus interaction, which can be targeted in future functional studies to enhance the resistance of Atlantic salmon to ISAV.
Article
Biotechnology & Applied Microbiology
Valentina Valenzuela-Munoz, Juan Antonio Valdes, Cristian Gallardo-Escarate
Summary: This study aimed to explore the transcriptional modulation of long non-coding RNAs during the freshwater to seawater transfer in Atlantic salmon. The results showed that most transcripts differently modulated were downregulated in all salinity conditions, and putative lncRNAs were identified with potential regulatory roles in smoltification-related genes. The lncRNAs may serve as novel biomarkers for the fish smoltification process, and specific lncRNAs like lncRNA_145326 and lncRNA_18762 are potentially related to the parr-smolt transfer in Atlantic salmon.
MARINE BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Thu Thi Minh Vo, Tuan Viet Nguyen, Gianluca Amoroso, Tomer Ventura, Abigail Elizur
Summary: The reduction in flesh pigmentation of farmed Atlantic salmon was investigated using RNA sequencing. Differential gene expression analysis between different flesh color phenotypes revealed associations with metal ion transport, oxidation-reduction processes, immune responses, and lipid metabolism. The study compared two library preparation methods and found similarities in the trend of differentially expressed genes validated by qPCR.
Article
Fisheries
Christine Klykken, Lauris Boissonnot, Anne Katrine Reed, Paul Whatmore, Kari Attramadal, Rolf Erik Olsen
Summary: Nephrocalcinosis is a common disorder in farmed Atlantic salmon and has significant physiological consequences, including decreased metabolism and energy production, upregulation of important signaling pathways, and increased inflammatory responses.
JOURNAL OF FISH DISEASES
(2022)
Article
Biotechnology & Applied Microbiology
Ophelie Gervais, Athina Papadopoulou, Remi Gratacap, Borghild Hillestad, Alan E. Tinch, Samuel A. M. Martin, Ross D. Houston, Diego Robledo
Summary: This study investigates the transcriptomic responses of ISAV-infected salmon with contrasting levels of resistance, revealing that the responses to ISAV are organ-specific with a strong immune response observed in the head kidney. Other biological processes such as ubiquitination and ribosomal processing also play a role during early infection.
Article
Biotechnology & Applied Microbiology
Scarleth Bravo, Francisco Leiva, Javier Moya, Osiel Guzman, Rodrigo Vidal
Summary: Sea lice are harmful marine parasites that pose a serious threat to the global salmon industry. Transcriptomic investigations have focused on gene expression response of Atlantic salmon to sea lice, but have overlooked the important role of alternative splicing (AS) in gene expression control. This study identified a significant increase in AS events and AS genes after sea lice infestation, and found a higher number of AS events and genes in resistant and susceptible phenotypes. Functional annotation of AS genes revealed their association with behavior, RNA splicing, immune response, and RNA binding.
MARINE BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Carlo C. Lazado, Marianne Iversen, Arvind Y. M. Sundaram
Summary: This study provides the first insight into the basal transcriptome of the olfactory rosette in Atlantic salmon. Comparative transcriptomic analysis with gills, another organ possessing MALT, reveals shared cytokine-cytokine interaction and neuroactive ligand-receptor interaction pathways. The immunological characteristics of both organs are further characterized by the overrepresentation of immune-related pathways, particularly important for pathogen recognition. The differential regulation of markers for interleukins, extracellular matrix, antimicrobial peptides, and complement highlights the immunological differences between the two organs. The basal transcriptome of the Atlantic salmon olfactory rosette is a valuable molecular toolbox for understanding nasal immunity in teleost fish.
Article
Immunology
Natasha A. Botwright, Amin R. Mohamed, Joel Slinger, Paula C. Lima, James W. Wynne
Summary: Through studying the pathogenesis of amoebic gill disease in Atlantic salmon, the localized and systemic immune responses of the host and parasite were identified, along with transcription factors and gene networks significantly altered in disease states. This dual transcriptomic study provides novel molecular insights into the pathology of AGD, offering alternative theories for future research and improved management strategies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Qiang Fu, Yuqing Li, Hao Zhang, Min Cao, Lu Zhang, Chengbin Gao, Xin Cai, Defeng Chen, Ziying Yang, Jie Li, Ning Yang, Chao Li
Summary: This study conducted RNA-seq analysis on spleen tissue of Atlantic salmon after Aeromonas salmonicida subsp. masoucida (ASM) vaccination to reveal genes, their expression patterns, and pathways involved in immune protections. The results showed that differentially expressed genes were involved in cytokine-cytokine receptor interaction, HTLV-I infection, MAPK signaling pathway, PI3K-Akt signaling pathway, and TNF signaling pathway. The study provided insights into gene regulation and pathways involved in immune response to the vaccine.
MARINE BIOTECHNOLOGY
(2022)
Article
Genetics & Heredity
Eric H. Ignatz, Tiago S. Hori, Surendra Kumar, Tillmann J. Benfey, Laura M. Braden, C. Dawn Runighan, Jillian D. Westcott, Matthew L. Rise
Summary: The study found that increasing rearing temperature has broad impacts on the biological systems of AquAdvantage Salmon, including stress response, immune system process, and lipid metabolic process. The high correlation between RT-qPCR results and RNA-seq results provides important information for determining the optimal rearing temperature for AquAdvantage Salmon.
FRONTIERS IN GENETICS
(2022)
Article
Anatomy & Morphology
Harald Kryvi, Kari Nordvik, Per Gunnar Fjelldal, Mariann Eilertsen, Jon Vidar Helvik, Eivind Nagel Storen, John H. Long
Summary: The study reveals significant differences in the structure of the notochord between the cranial and caudal regions in the development of Atlantic salmon. Previously undescribed physiological processes have been identified during development, affecting the structural changes of the notochord.
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
(2021)
Article
Fisheries
Pal A. Olsvik, Kristine E. Brokke, Ole B. Samuelsen, Rita Hannisdal
Summary: This study investigates the uptake and toxicity of Lufenuron in liver tissue of Atlantic salmon. The results show that Lufenuron easily bioaccumulates and induces a transient inhibition of transcription in the liver of salmon.
JOURNAL OF FISH DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Denise Rebello, Elizabeth Wohler, Vida Erfani, Guozhuang Li, Alexya N. Aguilera, Alberto Santiago-Cornier, Sen Zhao, Steven W. Hwang, Robert D. Steiner, Terry Jianguo Zhang, Christina A. Gurnett, Cathleen Raggio, Nan Wu, Nara Sobreira, Philip F. Giampietro, Brian Ciruna
Summary: Human vertebral malformations (VMs) are associated with health problems such as congenital scoliosis (CS) and syndromes like VACTERL. The genetic cause for most VMs is unknown. In this study, COL11A2 variants were identified in CS/VM patients, and experiments in zebrafish suggested a role for COL11A2 in vertebral development and a pathogenic role for these variants in CS.
HUMAN MOLECULAR GENETICS
(2023)