期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 53, 期 5, 页码 1731-1735出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.03094-14
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- Ministry of Health of the Czech Republic [NT11032-6/2010]
A comparison of a matrix-assisted laser desorption ionization-time of flight mass spectrometric (MALDI-TOF MS) meropenem hydrolysis assay with the Carba NP test showed that both methods exhibited low sensitivity (approximately 76%), mainly due to the false-negative results obtained with OXA-48-type producers. The addition of NH4HCO3 to the reaction buffer for the MALDI-TOF MS assay dramatically improved its sensitivity (98%). Automatic interpretation of the MALDI-TOF MS assay, using the MBT STAR-BL software, generally agreed with the results obtained after manual analysis. For the Carba NP test, spectrophotometric analysis found six additional carbapenemase producers.
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