Article
Microbiology
Corrie R. Belanger, Kerstin Locher, Billie Velapatino, Philippe J. Dufresne, Eric Eckbo, Marthe Charles
Summary: This study compared two commercial real-time PCR assays for the detection of Pneumocystis jirovecii. The results showed good concordance between the two methods for positive and negative samples, but discordant results occurred mainly in samples with low fungal loads.
MICROBIOLOGY SPECTRUM
(2023)
Article
Dermatology
Margot Salse, Victor Mercier, Marie-Jose Carles, Catherine Lechiche, Milene Sasso
Summary: The study evaluated the performance of the RealStar(R) P jirovecii PCR kit for PCP diagnosis, showing excellent concordance with an in-house PCR. The kit performed well in samples from patients with proven and probable PCP, but had a lower percentage of positive samples in the possible PCP category.
Article
Infectious Diseases
Willy W. Y. Ng, Yolanda I. I. Ho, Ann H. Wong, Eddie C. M. Leung, Alfred L. H. Lee, Viola C. Y. Chow
Summary: Both PneumID PCR and Amplex eazyplex LAMP assay enable accurate diagnosis of definite Pneumocystis jirovecii pneumonia (PJP). PneumID assay was more sensitive than eazyplex assay for detection of P. jirovecii. However, differentiation between probable PJP from colonization was not feasible.
Article
Microbiology
Cristina Veintimilla, Ana Alvarez-Uria, Pablo Martin-Rabadan, Maricela Valerio, Marina Machado, Belen Padilla, Roberto Alonso, Cristina Diez, Patricia Munoz, Mercedes Marin
Summary: This study compared the performance of real-time PCR and immunofluorescence assay (IFA) in a large microbiology laboratory. The PCR test showed higher sensitivity, specificity, PPV, and NPV for diagnosing PJP compared to the IFA. Therefore, PCR is a robust and reliable method for diagnosing PJP.
Article
Pathology
Shu Jin Tan, Todd Matthew Pryce, Erin Judith Haygarth, Peter Alan Boan
Summary: The study demonstrated the utility of a Pneumocystis jirovecii quantitative polymerase chain reaction (qPCR) in distinguishing disease from colonization, with varying sensitivity and specificity in HIV and non-HIV patients. Standardization of methods and definitions is necessary for improving generalizability of results.
Article
Microbiology
Susana Ruiz-Ruiz, Carolina A. Ponce, Nicole Pesantes, Rebeca Bustamante, Gianna Gatti, Viviana San Martin, Mireya Gutierrez, Pamela Borquez, Sergio L. Vargas, Fabien Magne, Enrique J. Calderon, Vicente Perez-Brocal, Andres Moya
Summary: A new real-time PCR assay using SYBR Green has been developed for the detection of low levels of Pneumocystis jirovecii with higher sensitivity. This assay targets two regions simultaneously using two primer sets, and it has been found to be more rapid and simpler compared to the current nested-PCR methods. The new assay was able to detect P. jirovecii in samples that were negative for both rounds of nested-PCR, demonstrating its ability to detect low levels of the pathogen.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Medicine, General & Internal
Xiaoxiao Lu, Jianhui Zhang, Wentao Ma, Lihua Xing, Hanbing Ning, Mengying Yao
Summary: The incidence of non-HIV-infected Pneumocystis Jirovecii Pneumonia (PJP) is increasing. This study found that metagenomic next-generation sequencing (mNGS) can diagnose PJP quickly and accurately, and has advantages over traditional detection methods. However, PJP can easily be ignored due to atypical symptoms and ambiguous imaging features.
FRONTIERS IN MEDICINE
(2022)
Article
Microbiology
Angel Moreno, David Epstein, Indre Budvytiene, Niaz Banaei
Summary: Pneumocystis jirovecii plasma cell-free DNA PCR can noninvasively detect Pneumocystis pneumonia, especially suitable for patients who cannot tolerate bronchoscopy.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Immunology
Li Liu, Mingjuan Yuan, Yi Shi, Xin Su
Summary: The study evaluated the performance of BAL mNGS and serum BDG in differentiating P. jirovecii colonisation and infection, finding that they are useful adjunct tests for this purpose.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Infectious Diseases
Juan Jiang, Xia Wang, Jian He, Donglei Liao, Xiaolu Deng
Summary: Real-time PCR showed a sensitivity of 100% and a specificity of 98.0% in diagnosing PCP in children, and it performed well in identifying co-pathogens. Sputum can be an alternative sample for PCR assay when bronchoscopic examination is not feasible in children.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Immunology
Romain Lecuyer, Nahema Issa, Benoit Tessoulin, Rose-Anne Lavergne, Florent Morio, Frederic Gabriel, Emmanuel Canet, Celine Bressollette-Bodin, Aurelie Guillouzouic, David Boutoille, Francois Raffi, Raphael Lecomte, Paul Le Turnier, Colin Deschanvres, Fabrice Camou, Benjamin Jean Gaborit
Summary: Respiratory cytomegalovirus (CMV) coinfection at diagnosis of Pneumocystis jirovecii pneumonia (PcP) is independently associated with increased 90-day mortality, specifically in HIV-negative patients. This suggests that CMV coinfection may have an adverse impact on the prognosis of PcP patients.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Critical Care Medicine
Daniele Roberto Giacobbe, Silvia Dettori, Vincenzo Di Pilato, Erika Asperges, Lorenzo Ball, Enora Berti, Ola Blennow, Bianca Bruzzone, Laure Calvet, Federico Capra Marzani, Antonio Casabella, Sofia Choudaly, Anais Dartevel, Gennaro De Pascale, Gabriele Di Meco, Melissa Fallon, Louis-Marie Galerneau, Miguel Gallego, Mauro Giacomini, Adolfo Gonzalez Saez, Luise Haensel, Giancarlo Icardi, Philipp Koehler, Katrien Lagrou, Tobias Lahmer, P. Lewis White, Laura Magnasco, Anna Marchese, Cristina Marelli, Mercedes Marin-Arriaza, Ignacio Martin-Loeches, Armand Mekontso-Dessap, Malgorzata Mikulska, Alessandra Mularoni, Anna Nordlander, Julien Poissy, Giovanna Russelli, Alessio Signori, Carlo Tascini, Louis-Maxime Vaconsin, Joel Vargas, Antonio Vena, Joost Wauters, Paolo Pelosi, Jean-Francois Timsit, Matteo Bassetti
Summary: This study describes the characteristics of ICU patients undergoing diagnostic workup for PJP, and finds that PJP is most common in non-HIV patients. The results also suggest that combining serum BDG with respiratory Pneumocystis PCR can improve the accuracy of PJP diagnosis.
Review
Infectious Diseases
Julien Senecal, Elizabeth Smyth, Olivier Del Corpo, Jimmy M. Hsu, Alexandre Amar-Zifkin, Amy Bergeron, Matthew P. Cheng, Guillaume Butler-Laporte, Emily G. McDonald, Todd C. Lee
Summary: The study systematically reviewed and meta-analyzed the performance of minimally invasive PCP detection tests, highlighting induced sputum as highly sensitive and specific. Other testing modalities also showed diagnostic promise. Promising minimally invasive PCP diagnostic tests are available, potentially reducing the need for invasive respiratory sampling and improving clinical diagnosis of PCP.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Multidisciplinary Sciences
Tae-Ok Kim, Jae-Kyeong Lee, Yong-Soo Kwon, Yu-Il Kim, Sung-Chul Lim, Min-Seok Kim, Bo Gun Kho, Cheol-Kyu Park, In-Jae Oh, Young-Chul Kim, Ha Young Park, Hong-Joon Shin
Summary: This study compared the clinical characteristics and prognoses between patients with and without immunocompromised conditions who developed Pneumocystis jirovecii pneumonia (PCP). The findings revealed that patients without immunocompromised conditions had higher mortality rates and delayed anti-PCP treatment was associated with increased 90-day mortality.
Article
Microbiology
Iacopo Franconi, Alessandro Leonildi, Gianluca Erra, Roberta Fais, Marco Falcone, Emilia Ghelardi, Antonella Lupetti
Summary: This study compared the performance of microscopy, molecular assays, and beta-D-glucan diagnosis in bronchoalveolar-lavage fluids from patients with suspected Pneumocystis jirovecii pneumonia. The results showed that molecular methods had higher sensitivity and shorter diagnostic time, which is important in diagnosing this rare but severe and rapidly progressive infection.
Article
Anesthesiology
Philippe Cuvillon, Sandrine Alonso, Joel L'Hermite, Vanessa Reubrecht, Lana Zoric, Nathalie Vialles, Jean Luc Faillie, Pascal Kouyoumdjian, Christope Boisson, Mathieu Raux, Olivier Langeron
Summary: This study compared intravenous oxycodone and morphine in terms of opioid-related adverse events (ORAES) after total hip arthroplasty, finding no significant difference in ORAES within the first 24 hours postoperatively.
ACTA ANAESTHESIOLOGICA SCANDINAVICA
(2021)
Article
Surgery
E. Boutry, M. M. Bertrand, J. Ripoche, S. Alonso, S. Bastide, M. Prudhomme
Summary: The study aimed to assess the difference in quality of life between colostomy patients practicing colonic irrigation (CI) and those who do not. The results showed that patients practicing CI had significantly higher quality of life scores compared to those who do not practice CI.
JOURNAL OF VISCERAL SURGERY
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Aymeric Hamard, Joel Greffier, Sophie Bastide, Ahmed Larbi, Takieddine Addala, Alexandre Sadate, Jean-Paul Beregi, Julien Frandon
Summary: The study compared the performance of ultra-low-dose CT (ULD CT) and radiographs in detecting suspected spinal or pelvic ring or hip fractures for minor trauma, finding that ULD CT had higher sensitivity for fracture detection with an effective dose comparable to radiographs.
EUROPEAN RADIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Marlene Buisson, Geraldine Leguelinel, Sophie Bastide, Jean Paul Beregi, Jean Marie Kinowski, Julien Frandon, Virginie Chasseigne
Summary: The study aimed to evaluate the impact of pharmaceutical interventions by clinical pharmacists in the PICC process. Through analysis and validation of PICC requests, the implementation of a clinical pharmacy activity led to improved patient care and ensured the safety of the PICC circuit.
EUROPEAN JOURNAL OF HOSPITAL PHARMACY
(2021)
Article
Multidisciplinary Sciences
Catherine Dunyach-Remy, Florian Salipante, Jean-Philippe Lavigne, Maxime Brunaud, Christophe Demattei, Alex Yahiaoui-Martinez, Sophie Bastide, Claire Palayer, Albert Sotto, Anthony Gelis
Summary: This pilot study aimed to characterize the evolution of the cutaneous microbiota of pressure ulcers in spinal cord injury patients. The results suggest that certain bacterial genera may be associated with wound degradation, with Proteus potentially representing a promising target in pressure ulcer management.
SCIENTIFIC REPORTS
(2021)
Article
Dermatology
Claire de Labachelerie, Emilie Viollet, Sandrine Alonso, Fabrice Nouvel, Sophie Bastide, Mylene Blot, Willy Fagart, Anthony Gelis, Arnaud Dupeyron
Summary: This study compared the pressure relief effects of the SUMMIT mattress and the NIMBUS 3 mattress. Using the Pressure Relief Index (PRI), it was found that the SUMMIT mattress had a better interface pressure distribution in older patients compared to the NIMBUS 3 mattress.
JOURNAL OF TISSUE VIABILITY
(2022)
Article
Clinical Neurology
Ismael Conejero, Laurent Collombier, Jorge Lopez-Castroman, Thibault Mura, Sandrine Alonso, Emilie Olie, Vincent Boudousq, Fabrice Boulet, Caroline Arquizan, Charlotte Boulet, Anne Wacongne, Camille Heitz, Christel Castelli, Stephane Mouchabac, Philippe Courtet, Mocrane Abbar, Eric Thouvenot
Summary: This study investigates the alterations of resting brain metabolism in motor functional neurological disorder patients and finds associations between brain activity and persistent disability as well as motor recovery.
Article
Radiology, Nuclear Medicine & Medical Imaging
Taki Eddine Addala, Aymeric Hamard, Fehmi Snene, Xavier Bobbia, Sophie Bastide, Asmaa Belaouni, Ahmed Larbi, Jean-Emmanuel De La Coussaye, Jean-Paul Beregi, Julien Frandon, Joel Greffier, Helene de Forges, Pierre-Geraud Claret
Summary: This study assessed the diagnostic sensitivity and specificity of ULD-CT versus digital radiographs in patients with extremity traumas. The results showed that ULD-CT had better detection performance for extremity fractures compared to digital radiographs, with a similar effective dose level.
QUANTITATIVE IMAGING IN MEDICINE AND SURGERY
(2022)
Article
Acoustics
Celine Fournil, Nicolas Boulet, Sophie Bastide, Benjamin Louart, Audrey Ambert, Caroline Boutin, Jean-Yves Lefrant, Laurent Muller, Claire Roger
Summary: This study compares the success and complication rates of internal jugular vein and axillary vein catheterization. The overall success rates for both approaches were 96% and 89% respectively, with a higher first puncture success rate and shorter procedure duration in the internal jugular vein approach.
JOURNAL OF CLINICAL ULTRASOUND
(2023)
Article
Nutrition & Dietetics
Sandrine Fiorella, Hanane Agherbi, Emilia El Houjeiry, Giovanni Castelnovo, Dimitri Renard, Pauline Privat, Elodie Santamaria, Virginie Vallayer, Sandrine Alonso, Thierry Chevallier, Candice Bancal, Sabine Laurent-Chabalier, Eric Thouvenot
Summary: This study aimed to evaluate the efficacy of a personalized nutritional advice program in optimizing dietary calcium intake in patients with multiple sclerosis. The results showed that the personalized advice program was effective in increasing calcium consumption in these patients.
FRONTIERS IN NUTRITION
(2023)
Article
Emergency Medicine
Laura Grau-Mercier, Romain Genre Grandpierre, Sandrine Alonso, Alice Savey, Anne Le Floch, Fabien de Oliveira, Thibaud Masia, Nathalie Jory, Fabien Coisy, Pierre-Geraud Claret
Summary: This study aimed to evaluate the diagnostic performance of S100B serum assay in distinguishing primary and secondary headaches among patients with non-traumatic headaches in emergency departments. The results showed that the S100B protein assay was significantly higher in secondary headaches than primary headaches, with a calculated threshold and certain sensitivity and specificity. Therefore, S100B protein assay has value in the management of headaches in emergency settings.
AMERICAN JOURNAL OF EMERGENCY MEDICINE
(2023)
Article
Physiology
Laurent Maimoun, Krishna Kunal Mahadea, Sandrine Alonso, Thierry Chevallier, Pierre-Olivier Kotzki, Thibault Mura, Vincent Boudousq
Summary: This study assessed the agreement and precision between the Stratos DR and Discovery A densitometers for measuring body composition parameters. The results showed a high correlation between the two devices, but significant bias in specific measurements. The Stratos DR underestimated whole-body bone mineral density, fat mass, and fat-free soft tissue, while overestimating trunk fat mass and visceral adipose tissue. Overall, the Stratos DR demonstrated good precision but requires the use of cross-calibration equations.
CLINICAL PHYSIOLOGY AND FUNCTIONAL IMAGING
(2023)
Article
Medicine, General & Internal
Muriel Chazel, Sandrine Alonso, Jane Price, Sarah Kabani, Christophe Demattei, Pascale Fabbro-Peray
Summary: This study aimed to evaluate the number and risk factors of violent events encountered in the emergency department. A total of 82 periods were observed between November 2015 and April 2016, recording 35 violent incidents affecting 37 perpetrators and 48 victims. No significant risk factors for violent incidents were found in the analysis. The study highlights the high rate of violence in the emergency department and the need for strategies to prevent and reduce incidents.