Article
Plant Sciences
Han Xu, Dan Luo, Fan Zhang
Summary: The study identified a key transcription factor, DcWRKY75, in carnation flowers that plays a crucial role in ethylene-induced petal senescence by regulating the expression of ethylene biosynthetic genes and senescence-associated genes. Additionally, it was found that DcWRKY75 is a direct target gene of DcEIL3-1, which interacts with DcWRKY75 to regulate ethylene-induced petal senescence in carnation.
Article
Biochemistry & Molecular Biology
Taro Harada, Itsuku Horiguchi, Sayaka Ueyama, Ai Murai, Chie Tsuzuki
Summary: This study illustrates the effects of sucrose treatment and hypoxic treatment on starch content, enzyme activities, and gene expression in carnation petals. The findings reveal how sugar metabolism in carnation petals is regulated in response to environmental cues, with modulation of activities and gene expression of a set of sucrolytic enzymes.
Article
Agronomy
Nour El Houda Lezoul, Maria Serrano, Maria Celeste Ruiz-Aracil, Mohamed Belkadi, Salvador Castillo, Daniel Valero, Fabian Guillen
Summary: The study found that melatonin (MT) can extend the vase life of cut carnations, with 0.1 mM concentration of MT showing the most significant effect. While all concentrations of MT maintained initial levels for a longer period, lower concentrations had a more significant impact on vase life.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Plant Sciences
Han Xu, Siqi Wang, Robert M. Larkin, Fan Zhang
Summary: This study reveals the importance of antagonistic transcription factors in carnation petal senescence. The transcription factors DcHB30 and DcWRKY75 competitively regulate the expression of their co-targeted genes, thereby integrating ethylene signaling and regulating petal senescence.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Heling Jiang, Xiaoni Zhang, Luhong Leng, Desheng Gong, Xiaohui Zhang, Junyang Liu, Dan Peng, Zhiqiang Wu, Yingxue Yang
Summary: In this study, the haplotype-resolved genome of D. caryophyllus 'Aili' was assembled for the first time at the chromosome level. The lengths of the two assembled haplotypes were determined, and structural variations between the haplotypes were identified. Phylogenetic analysis revealed the species differentiation time between carnation and gypsophila to be approximately 54.43 million years ago. This assembled and annotated genome will facilitate molecular biology studies and contribute to genome-level evolutionary research.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Longna Li, Qianlan Yin, Tong Zhang, Pengfei Cheng, Sheng Xu, Wenbiao Shen
Summary: The study found that the use of hydrogen nanobubble water significantly prolonged the vase life of cut carnation flowers, reduced flower loss, and inhibited the activity of senescence-associated enzymes, thereby delaying the aging process of petals.
Article
Plant Sciences
Yuying Sun, Diandian Hu, Pengcheng Xue, Xueli Wan
Summary: In this study, 33 heat shock protein 20 (Hsp20) genes were identified and characterized from the carnation genome. The gene structure and cis-regulatory element analysis provided insights into the structure and regulation of these genes. Gene expression analysis and overexpression experiments demonstrated that one of the genes, DcHsp17.8, plays a crucial role in improving heat tolerance in plants.
Article
Agronomy
Poonam Sharma, Puja Sharma, Sita Ram Dhiman, Rajesh Kumar Dogra, Anju Sharma, Nepu Rana
Summary: The study analyzed the variability and association among mutants of carnation in Northwestern Himalayas. It found that the number of petals per flower had high heritability and can be used as an important trait for breeding.
GENETIC RESOURCES AND CROP EVOLUTION
(2023)
Article
Plant Sciences
Chunlin Zhu, Zhiheng Huang, Zheng Sun, Shan Feng, Siqi Wang, Teng Wang, Xinyi Yuan, Linlin Zhong, Yunjiang Cheng, Manzhu Bao, Fan Zhang
Summary: This study reveals the mutual regulation between DcEBF1/2 and DcEIL3-1 during ethylene induced petal senescence in carnation. Silencing of DcEBF1 and DcEBF2 accelerates ethylene induced petal senescence, while overexpression of DcEBF1 and DcEBF2 delays this process. The findings not only expand our understanding of the ethylene signal regulation network in carnation petal senescence, but also provide potential targets for breeding a cultivar of long-lived cut carnation.
Article
Agronomy
Wenjing Yu, Yuan Tao, Liping Luo, Jonathan Hrovat, Ahui Xue, Huolin Luo
Summary: In this study, three computational statistical methods were used to evaluate the expression stability of 12 commonly-used reference genes in Dianthus caryophyllus. The results showed significant variations in the expression stability of candidate genes across different experimental conditions. TIP41 and UBQ10 were identified as relatively stable reference genes, while CYP and TUA could serve as reliable internal controls. Additionally, certain gene pairs were found to be suitable reference genes under specific treatments, such as hormone, heavy metal, salt, heat, cold, flooding, and drought.
NEW ZEALAND JOURNAL OF CROP AND HORTICULTURAL SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Zheng Sun, Manman Wu, Siqi Wang, Shan Feng, Yan Wang, Teng Wang, Chunlin Zhu, Xinyu Jiang, Hongya Wang, Ruiming Wang, Xinyi Yuan, Menglu Wang, Linlin Zhong, Yunjiang Cheng, Manzhu Bao, Fan Zhang
Summary: This study identified a new mechanism involving post-transcriptional regulation by the DcNAP transcription factor in carnation petal senescence. The study revealed the different roles of multiple splice variants of DcNAP in the senescence process, which may provide a useful strategy for crop genetic improvement.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Pooja Sharma, Amarjit K. Nath, Sita Ram Dhiman, Sheetal Dogra, Vishal Sharma
Summary: Carnation genotypes and mutants were characterized using PCR-based DNA markers, with RAPD, ISSR, and SSR showing different levels of polymorphism among the genotypes. Cluster analysis separated the genotypes and mutants into distinct groups, and ISSR and SSR markers displayed higher effectiveness in detecting genetic variability. This study highlights the potential of SSR markers in breeding programs and varietal identification in carnations.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Plant Sciences
Maryam Dehestani-Ardakani, Jalal Gholamnezhad, Samaneh Alizadeh, Heidar Meftahizadeh, Mansour Ghorbanpour
Summary: This study evaluated the effects of Salix and Mentha leaf extracts and salicylic acid on the post-harvest quality and vase life of cut carnations. The results showed that all treatments significantly prolonged the vase life and increased membrane stability, protein, and antioxidant enzyme activity. Salicylic acid treatment had the greatest improvement on the longevity of the flowers.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Plant Sciences
Janneth Santos-Rodriguez, Ericsson Coy-Barrera, Harold Duban Ardila
Summary: The soil application of a biotic elicitor, eFod, can effectively reduce the incidence of vascular wilt in carnation and increase the accumulation of specific phenolic compounds.
Article
Plant Sciences
Teng Wang, Zheng Sun, Siqi Wang, Shan Feng, Ruiming Wang, Chunlin Zhu, Linlin Zhong, Yunjiang Cheng, Manzhu Bao, Fan Zhang
Summary: In this study, it was found that ethylene treatment significantly upregulated the gene encoding WRKY transcription factor DcWRKY33. Silencing and overexpression of DcWRKY33 could delay and accelerate the senescence of carnation petals, respectively. ABA and H2O2 treatments also accelerated petal senescence by inducing the expression of DcWRKY33. Furthermore, DcWRKY33 directly binds to the promoters of ethylene and ABA biosynthesis genes and the ROS generation gene to activate their expression. Ethylene, ABA, and ROS were found to have relationships with each other. This study elucidated the role of DcWRKY33 in petal senescence and provides insights for prolonging the vase life of cut carnation.