Article
Veterinary Sciences
Jiyuan Shen, Zhiyun Hao, Yuzhu Luo, Huimin Zhen, Yan Liu, Jiqing Wang, Jiang Hu, Xiu Liu, Shaobin Li, Zhidong Zhao, Yuan Liu, Shutong Yang, Longbin Wang
Summary: This study compared the expression profiles of miRNAs in the Longissimus dorsi muscle tissue between two goat breeds and identified differentially expressed miRNAs that may play important roles in the phenotype differences in meat production performance.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Reproductive Biology
Jin-Shan Ran, Ling-Qian Yin, Jing-Jing Li, Yan-Qiang Tang, Jian Huang, Peng Ren, Xian-Xian Zhang, Si-Ming Li, Yi-Ping Liu
Summary: This study identified differentially expressed miRNAs and mRNAs involved in ovarian atrophy through high throughput sequencing. Functional analysis and interaction network construction revealed key regulators in ovary development. These findings expanded our understanding of the molecular mechanism underlying ovarian atrophy.
Article
Agriculture, Dairy & Animal Science
Shuang Sun, Ming Lv, Huimin Niu, Jun Luo
Summary: This study investigated the molecular mechanisms behind the reduced reproductive performance of dairy goats after repeated estrus synchronization treatments. The results showed that the goats receiving multiple treatments had lower estrus rate and litter size compared to those receiving only one treatment. The AQP3 gene was found to be involved in reducing the reproductive performance of these goats. These findings provide a theoretical foundation for the effective use of reproductive hormones in livestock breeding techniques.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Agriculture, Dairy & Animal Science
Qiongxian Yan, Lina Tian, Wenxun Chen, Jinhe Kang, Shaoxun Tang, Zhiliang Tan
Summary: The colon plays a crucial role in the fermentation and utilization of cell wall carbohydrates in ruminants. MicroRNAs have been found to regulate gastrointestinal development and maintain intestinal homeostasis in mammals. This study investigated the expression profiles of microRNAs in the colon of kid goats and identified different roles for DEmiRNAs, HEmiRNAs, and dynamic microRNAs in colon development. The targeted genes regulated by these microRNAs were associated with microbial invasion prevention, barrier maintenance, and cell damage surveillance. The study also revealed the impact of age on microRNA expression in the colon. Overall, this research provides new insights into the regulatory mechanism of colon development in goats.
Article
Biochemistry & Molecular Biology
Yuting Zhang, Yu Jiao, Yiping Li, Qunli Tian, Xiaodong Du, Yuewen Deng
Summary: This study identified and analyzed miRNA molecules in mantle tissues of Pinctada fucata martensii, shedding light on the regulatory mechanisms involved in shell formation. Over-expression of specific miRNAs led to disordered growth of nacre, providing insights into the potential targets of these miRNAs in shell formation.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2021)
Article
Microbiology
Long-Ping Li, Lei Qu, Tuo Li
Summary: This study investigated the effects of dietary supplementation of Selenohomolanthionine (SeHLan) on the growth and rumen microbiota of cashmere goats. The results showed that medium SeHLan supplementation significantly affected rumen bacterial composition, nutrient digestibility, and growth of the goats.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Beining Kang, Junjie Wang, Hongfu Zhang, Wei Shen, Othman El-Mahdy Othman, Yong Zhao, Lingjiang Min
Summary: This study explored DNA methylation status in ovaries of high and low litter size goats, finding global differences and many differentially methylated region-related genes. Enrichment analysis indicated involvement of these genes in gamete development, reproductive system development, WNT signaling pathways, and MAPK signaling pathways. The study suggests that DNA methylation in goat ovaries may be important for folliculogenesis, oocyte ovulation rate, and litter size.
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
(2022)
Article
Microbiology
Yi Huang, Md Roushan Ali, Wei Li, Wanying Wang, Yuanyuan Dai, Huaiwei Lu, Zhien He, Yujie Li, Baolin Sun
Summary: This study focused on Acinetobacter baumannii, a major health threat, and specifically investigated the ST369 strain prevalent in China. The researchers identified potential mutations that could impact bacterial virulence and resistance, providing a foundation for further research on clinical isolates harboring similar mutations.
Article
Genetics & Heredity
Xiaodong Zhao, Zhibin Ji, Rong Xuan, Aili Wang, Qing Li, Yilin Zhao, Tianle Chao, Jianmin Wang
Summary: This study sequenced miRNAs in the liver tissues of goat kids and investigated their regulation roles in early liver development. The results showed that differential expression miRNAs played important roles in liver development, lipid metabolism, and toxin-related metabolism. Furthermore, miRNA-mRNA regulation network analysis revealed 9 miRNAs with significant regulatory roles.
FRONTIERS IN GENETICS
(2022)
Article
Agriculture, Dairy & Animal Science
Chao-Nan Huang, Cheng-Li Liu, Shi-Qi Zeng, Chang-Bao Liu, Wei-Jiang Si, Ying Yuan, Li-Xin Ren, Yong-Meng He, Wei-Yi Zhang, Hao-Yuan Zhang, Yan Zeng, Yan-Guo Han, Ri-Su Na, Guang-Xin Ee, Yong-Fu Huang
Summary: This study used RNA-Seq technology to analyze differentially expressed long non-coding RNAs (DELs) and differentially expressed genes (DEGs) in goat muscle at different developmental stages and identified mRNAs and lncRNAs related to muscle development. The results of this study can provide reference for understanding the genetic basis and molecular mechanism of muscle development in goats.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Obstetrics & Gynecology
Xiao Jiang, Jingyu Li, Bingqian Zhang, Jingmei Hu, Jinlong Ma, Linlin Cui, Zi-Jiang Chen
Summary: This study investigated the different expression profiles of plasma exosomal miRNAs in PCOS patients and controls, and their potential roles in PCOS pathogenesis. Through sequencing and analysis, differentially expressed miRNAs were identified, which may contribute to the understanding of PCOS pathophysiology. The expression of certain miRNAs correlated with clinical features of PCOS and could help distinguish PCOS patients from controls.
FERTILITY AND STERILITY
(2021)
Article
Biochemistry & Molecular Biology
J. Nikhil Kumar Tej, P. Johnson, Kavya Krishna, Kalpana Kaushik, P. S. P. Gupta, S. Nandi, S. Mondal
Summary: The study investigated the effects of copper and selenium on mRNA expression of genes associated with estradiol synthesis in caprine ovarian granulose cell models. Results indicated a higher expression of several genes in groups treated with copper and copper combined with selenium compared to the control group, suggesting a key role of copper and selenium in activating AKT and WNT signaling pathways leading to an increase in mRNA expression of CYP19A1.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Xin Liu, Yuehua Zhu, Siyuan Zhan, Tao Zhong, Jiazhong Guo, Jiaxue Cao, Li Li, Hongping Zhang, Linjie Wang
Summary: This study provides a detailed landscape of miRNA expression in goat brown adipose tissue (BAT) and white adipose tissue (WAT). It is found that miR-433 acts as a negative regulator in controlling brown adipocyte differentiation and thermogenesis in goats.
Article
Genetics & Heredity
Yuchun Xie, Zhihong Liu, Juntao Guo, Xin Su, Cun Zhao, Chongyan Zhang, Qing Qin, Dongliang Dai, Yanhong Zhao, Zhiying Wang, Ruijun Wang, Yanjun Zhang, Rui Su, Zhixin Wang, Jinquan Li
Summary: The study identified important regulatory mechanisms of fatty acid metabolism in cashmere goat meat through transcript-level sequencing of miRNAs and mRNAs, revealing key factors involved in PUFA synthesis and metabolism.
FRONTIERS IN GENETICS
(2021)
Article
Agriculture, Dairy & Animal Science
Mingzhe Fu, Xiaolong Xu, Zefang Cheng, Junru Zhu, Ao Sun, Ganggang Xu, Xiaopeng An
Summary: This study analyzed the transcriptome and proteome of dairy goats infected with Corynebacterium pseudotuberculosis. The results showed that in the early stage of infection, dairy goats can defend against bacterial invasion through multiple pathways. However, in the later stages of infection, a few gene-protein pairs play a role in protein processing in the endoplasmic reticulum pathway. The mRNA and protein expressions of dairy goats indicate their adaptability to the bacteria, providing guidance for treatment and feeding management according to different periods of time.
Article
Agriculture, Dairy & Animal Science
Zubing Cao, Dandan Zhang, Yiqing Wang, Xu Tong, Lourdes Felicidad Cordova Avalos, Ibrar Muhammad Khan, Di Gao, Tengteng Xu, Ling Zhang, Jason G. Knott, Yunhai Zhang
ANIMAL REPRODUCTION SCIENCE
(2020)
Article
Reproductive Biology
Zubing Cao, Ling Zhang, Renyun Hong, Yunsheng Li, Yiqing Wang, Xin Qi, Wei Ning, Di Gao, Tengteng Xu, Yangyang Ma, Tong Yu, Jason G. Knott, Anucha Sathanawongs, Yunhai Zhang
Summary: METTL3 sustains blastocyst development in pigs by inhibiting autophagy.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biochemical Research Methods
Xin Shan, Tong Yu, Xu Yan, Jianling Wu, Yani Fan, Xiaoyu Guan, Fugui Fang, Yinghui Lin, Yunhai Zhang, Yunsheng Li, Ya Liu
Summary: This study identified proteins and pathways involved in porcine follicular atresia through protein labeling and mass spectrometry analysis, finding 399 differentially abundant proteins between healthy and atretic follicles. These results establish a foundation for elucidating the mechanism of follicular atresia in swine.
JOURNAL OF PROTEOMICS
(2021)
Article
Cell Biology
Lei Luo, Yanna Dang, Yan Shi, Panpan Zhao, Yunhai Zhang, Kun Zhang
Summary: SIN3A is a central scaffold protein involved in the regulation of HDAC1 expression during early embryonic development in mammals. The maternal origin of SIN3A was confirmed in pigs, cattle, mice, and humans, with its depletion causing developmental arrest in pigs but not in bovines. Furthermore, SIN3A affects CCNB1 expression, which is crucial for embryonic development, and can be partially rescued by exogenous CCNB1 mRNA injection.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Veterinary Sciences
Ibrar Muhammad Khan, Zubing Cao, Hongyu Liu, Adnan Khan, Sajid Ur Rahman, Muhammad Zahoor Khan, Anucha Sathanawongs, Yunhai Zhang
Summary: Sperm cryopreservation is a powerful technique in livestock breeding, but mammalian sperm are susceptible to cryo-injury. Various OMICS technologies are being used to improve the quality of frozen-thawed spermatozoa, demonstrating the importance of understanding the cryo-injury mechanism for optimizing the cryopreservation process.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Fei Zhang, Xiaodong Zhang, Wei Ning, Xiangdong Zhang, Zhenyuan Ru, Shiqi Wang, Mei Sheng, Junrui Zhang, Xueying Zhang, Haiqin Luo, Xin Wang, Zubing Cao, Yunhai Zhang
Summary: Circular RNAs (circRNAs) are abundantly expressed in boar testes and exhibit dynamic changes during testicular development. They are mainly involved in regulating spermatogenesis, cilia motility, and hormone biosynthesis, which indicates their potential as molecular markers for breeding of elite boars and evaluating developmental status of boar testes.
Article
Agriculture, Dairy & Animal Science
Hongyu Liu, Ibrar Muhammad Khan, Huiqun Yin, Xinqi Zhou, Muhammad Rizwan, Jingyi Zhuang, Yunhai Zhang
Summary: This study comprehensively analyzed the expression profiles of mRNAs and long non-coding RNAs in the testes of bulls of two different age groups, providing insights into the differences in sexual development caused by changes in mRNA and long non-coding RNA interactions between immature and mature stages.
Article
Cell Biology
Zubing Cao, Xu Tong, Huiqun Yin, Naru Zhou, Xiangdong Zhang, Mengya Zhang, Xin Wang, Qiuchen Liu, Yelian Yan, Yangyang Ma, Tong Yu, Yunsheng Li, Yunhai Zhang
Summary: The study revealed that CARM1 specifically catalyzes H3R26me2 in porcine embryos to promote blastocyst formation and participates in blastocyst development. Functional studies using chemical inhibition and RNA interference demonstrated that inhibition or knockdown of CARM1 severely compromised blastocyst development and altered the transcriptome characterized by downregulation of key genes associated with Hippo and PI3K-AKT signaling pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Tengteng Xu, Chengxue Liu, Mengya Zhang, Xin Wang, Yelian Yan, Qiuchen Liu, Yangyang Ma, Tong Yu, Anucha Sathanawongs, Jun Jiao, Zubing Cao, Yunhai Zhang
Summary: This study found that vitrification reduces the developmental efficiency of pig blastocysts by decreasing transcriptional activity during embryonic genome activation and disrupting gene expression. These findings suggest that vitrification impairs genome activation in pig embryos.
Article
Genetics & Heredity
Heming Sui, Shiqi Wang, Gang Liu, Fei Meng, Zubing Cao, Yunhai Zhang
Summary: Heat stress has significant negative effects on boar semen, reducing sperm motility and altering metabolomic profiles.
Article
Agriculture, Dairy & Animal Science
Qiuchen Liu, Mengya Zhang, Tenglong Guo, Sucheng Wu, Yanfeng Zong, Changzhi Xu, Zhihua Zhu, Yunhai Zhang, Zubing Cao
Summary: In this study, differentially expressed circRNAs (DECs) were identified in the blood of the distinctive local breed of Chinese Jianghuai buffalo, and these DECs were mainly enriched in the epidermal growth factor receptor-signaling pathway important for embryonic development and pregnancy maintenance. These findings provide a better understanding of buffalo pregnancy establishment and a potential basis for improving early pregnancy diagnosis techniques in buffalo.
Article
Genetics & Heredity
Hongyu Liu, Ibrar Muhammad Khan, Yong Liu, Nazir Muhammad Khan, Kaiyuan Ji, Huiqun Yin, Wenliang Wang, Xinqi Zhou, Yunhai Zhang
Summary: This study detected the expression of miRNAs in bovine testes using high-throughput sequencing technology and identified miRNAs and genes associated with testicular development and spermatogenesis through bioinformatics analysis. The research provides important insights into the role of miRNAs in testicular development and spermatogenesis.
Article
Biochemistry & Molecular Biology
Mengya Zhang, Xin Wang, Qiuchen Liu, Yelian Yan, Changzhi Xu, Zhihua Zhu, Sucheng Wu, Xiaoyu Zuo, Yanfeng Zong, Chunxiao Li, Yunhai Zhang, Zubing Cao
Summary: 3-MC exposure has toxic effects on the maturation of oocytes and development of embryos. It inhibits cumulus expansion and polar body extrusion, reduces cleavage and blastocyst rates, induces spindle abnormalities and chromosomal misalignments, and alters the levels of mitochondria, cortical granules, acetylated alpha-Tubulin, reactive oxygen species, DNA damage, and apoptosis. Abnormal gene expression related to cumulus expansion and apoptosis is also observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Zoology
Zu-Bing Cao, Di Gao, Hui-Qun Yin, Hui Li, Teng-Teng Xu, Meng-Ya Zhang, Xin Wang, Qiu-Chen Liu, Ye-Lian Yan, Yang-Yang Ma, Tong Yu, Yun-Sheng Li, Yun-Hai Zhang
Summary: INO80 plays a crucial role in porcine pre-implantation embryonic development by promoting blastocyst formation and regulating lineage allocation. It functions by modulating the expression of key genes involved in tight junction assembly and fluid accumulation during blastocyst development.
ZOOLOGICAL RESEARCH
(2021)
Article
Zoology
Ibrar Muhammad Khan, Dezhi Xu, Zubing Cao, Hongyu Liu, Adnan Khan, Sajid Ur Rahman, Jam Zaheer Ahmed, Muhammad Akmal Raheem, Yunhai Zhang
Summary: Vitamins and amino acids are essential for body growth and reproductive soundness. The addition of antioxidants like L-cysteine and vitamin E improved the quality of freeze-thawed spermatozoa in crossbred bulls.
PAKISTAN JOURNAL OF ZOOLOGY
(2021)
