Article
Multidisciplinary Sciences
Keisuke Maezono, Shintaro Kobayashi, Koshiro Tabata, Kentaro Yoshii, Hiroaki Kariwa
Summary: Deletion of the C-terminal region of E protein suppressed the formation of subviral particles (SPs) without affecting E protein production. The sensitivity of ELISA using full-length E protein was higher than that of truncated E proteins. ELISA using full-length E protein showed little cross-reactivity with anti-JEV antibodies and had similar sensitivity to the neutralization test.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Swee Ling Low, Yee Sin Leo, Yee Ling Lai, Sally Lam, Hwee Huang Tan, Judith Chui Ching Wong, Li Kiang Tan, Lee Ching Ng
Summary: Through evaluating the performance of eight commercially available ZIKV IgM and IgG assays on three testing platforms, it was found that these assays have varying sensitivities and specificities, with Diapro ZIKV IgM ELISA and Euroimmun ZIKV IgG ELISA showing high sensitivity and specificity in certain sample types.
Article
Immunology
Bailey E. Maloney, Kassandra L. Carpio, Ashley N. Bilyeu, Danielle R. D. Saunders, So Lee Park, Adrienne E. Pohl, Natalia Costa Ball, Janae L. Raetz, Claire Y. Huang, Stephen Higgs, Alan D. T. Barrett, Gleyder Roman-Sosa, Joanie L. Kenney, Dana L. Vanlandingham, Yan-Jang S. Huang
Summary: Multiple conserved hydrophobic residues in the EDI-EDII hinge region of the flavivirus envelope protein play a critical role in the conformational change of E protein and viral entry, with some residues contributing to virulence phenotype.
Article
Microbiology
Zhancheng Tian, Hongge Zhang, Ruiming Yu, Junzheng Du, Shandian Gao, Qiongjie Wang, Guiquan Guan, Hong Yin
Summary: The levels of swine GBP1 (sGBP1) were increased at the late stage of Japanese encephalitis virus (JEV) infection. Overexpression of sGBP1 inhibited JEV production, while knockdown of sGBP1 promoted JEV production. The GTPase activity and isoprenylation of sGBP1 were both critical for its anti-JEV activity.
VETERINARY MICROBIOLOGY
(2023)
Article
Immunology
Robert E. Snyder, Gail Sondermeyer Cooksey, Vicki Kramer, Seema Jain, Duc J. Vugia
Summary: WNV-associated hospitalizations in California were substantial and costly, with higher incidence in males, elderly persons, and patients with underlying conditions. WNV remains a costly and severe public health threat in California.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Microbiology
Qiuyan Li, Dengyuan Zhou, Fan Jia, Luping Zhang, Usama Ashraf, Yunchuan Li, Hongyu Duan, Yunfeng Song, Huanchun Chen, Shengbo Cao, Jing Ye
Summary: The study reveals a novel mechanism by which the JEV NS19 protein interacts with the cellular CDK1 protein to facilitate JEV replication by dampening the cellular antiviral response. Inhibition of CDK1 activity significantly suppresses JEV replication, providing a new therapeutic option for treating JEV infection.
MICROBIOLOGY SPECTRUM
(2021)
Article
Medicine, General & Internal
Geovana M. Pereira, Erika R. Manuli, Laurie Coulon, Marina F. Cortes, Mariana S. Ramundo, Loic Dromenq, Audrey Larue-Triolet, Frederique Raymond, Carole Tourneur, Carolina dos Santos Lazari, Patricia Brasil, Ana Maria Bispo de Filippis, Glaucia Paranhos-Baccala, Alice Banz, Ester C. Sabino
Summary: This international study evaluated the performance of the automated VIDAS® anti-CHIKV IgM and IgG assays compared to that of manual competitor IgM and IgG ELISA for the detection of anti-CHIKV IgM and IgG antibodies in patients with suspected CHIKV infection. The VIDAS® assays showed high accuracy and sensitivity, with positive and negative agreements ranging from 97.5% to 100.0%, and a high detection rate for IgM and IgG antibodies. The study also demonstrated the superiority of ELISA and VIDAS® assays over rapid diagnostic tests.
Article
Virology
Tao Chen, Shuo Zhu, Ning Wei, Zikai Zhao, Junjun Niu, Youhui Si, Shengbo Cao, Jing Ye
Summary: This study developed a modified mRNA vaccine for Japanese encephalitis virus (JEV) and demonstrated its efficacy in a mouse model. The vaccine induced strong immune responses and protected mice from JEV infection. This provides a new option for JE vaccination and lays the foundation for future development of more efficient and safer JEV mRNA vaccines.
Article
Microbiology
Tomokazu Tamura, Shiho Torii, Kentaro Kajiwara, Itsuki Anzai, Yoichiro Fujioka, Kisho Noda, Shuhei Taguwa, Yuhei Morioka, Rigel Suzuki, Yuzy Fauzyah, Chikako Ono, Yusuke Ohba, Masato Okada, Takasuke Fukuhara, Yoshiharu Matsuura
Summary: Flaviviruses and related viruses pose a major threat to public health. This study reveals the role of secretory NS1 in the particle formation of flaviviruses, which is achieved through its interaction with the lipid membrane.
Letter
Immunology
Gino Arcilla, Antoine Nguyen, Antonio Liu
Summary: We encountered a patient with HIV who showed rapid deterioration in mental status and was positive for both acute West Nile encephalitis and amphiphysin antibodies. After reviewing the literature, we found Dr. Moutsopoulos's paper from your journal highly interesting (Karagianni et al., 2019 [1]). Although autoimmune encephalitis following West Nile encephalitis is not uncommon, there are several intriguing aspects in our patient's case. Firstly, the coexistence of amphiphysin antibodies with West Nile encephalitis has not been reported before. Secondly, the monophasic clinical course suggests that autoimmune encephalitis triggered by or coexisting with West Nile encephalitis may be significantly underrecognized. Lastly, our patient was HIV positive but not severely immunocompromised, which may have contributed to the autoimmune status.
CLINICAL IMMUNOLOGY
(2023)
Article
Virology
Guohua Li, Xianyong Meng, Zhiguang Ren, Entao Li, Feihu Yan, Jing Liu, Ying Zhang, Zhanding Cui, Yuetao Li, Hongli Jin, Zengguo Cao, Le Yi, Pei Huang, Hang Chi, Hualei Wang, Weiyang Sun, Tiecheng Wang, Yuwei Gao, Yongkun Zhao, Songtao Yang, Xianzhu Xia
Summary: West Nile virus disease is caused by the arthropod-borne West Nile virus, posing a significant public health risk. The constructed ChiVax-WN01 chimera exhibits distinct characteristics compared to other strains, with moderate safety and immunogenicity demonstrated in animal studies.
Article
Immunology
Esther Gomez-Vicente, Rafael Garcia, Elizabeth Calatrava, Maria Jose Olivares Duran, Juan Francisco Gutierrez-Bautista, Javier Rodriguez-Granger, Fernando Cobo, Jose Maria Navarro Mari, Antonio Sampedro-Martinez
Summary: This study compared the agreement, sensitivity, and specificity of two different methods for detecting West Nile virus (WNV) infection. The results showed that the WNV VIRCLIA IgM and IgG monotest offers accurate detection of WNV in serum and cerebrospinal fluid (CSF) specimens.
Article
Immunology
Yoshua Colyn Collins-Sawaragi, Patra Koletsi, Eleanor Elizabeth Donlevy, Simon Bruce Drysdale
Summary: This article presents a case of initial seronegative West Nile virus encephalitis in an immunocompromised child and highlights that diagnostic guidelines may not be applicable to immunocompromised patients.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2022)
Article
Medicine, General & Internal
Steev Loyola, Alfredo Huaman, Dina Popuche, Elizabeth Castillo, Julia S. Ampuero, Maria Silva, Carolina Guevara, Douglas M. Watts
Summary: The study evaluated the performance of MAC-ELISA and PRNT for diagnosing ZIKV infection in dengue-endemic areas, with both assays showing high sensitivity and specificity. Restricting the analysis according to neutralizing antibodies against dengue or IgM antibodies, the performances of both serological assays were adequate.
Article
Infectious Diseases
Tommaso Lupia, Valentina Libanore, Silvia Corcione, Valentina Fornari, Barbara Rizzello, Roberta Bosio, Giacomo Stroffolini, Paolo Bigliano, Silvia Fontana, Francesca Patti, Maria Teresa Brusa, Maria Degioanni, Erika Concialdi, Anna Sara Navazio, Maurizio Penna, Francesco Giuseppe De Rosa
Summary: This study reported a case series of West Nile virus infection in Italy in 2018, focusing on the clinical characteristics and sequelae in patients. It highlighted the importance of environmental conditions and bacterial superinfections in the outbreak of this virus.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2022)
Article
Geriatrics & Gerontology
Pi-Shan Hsu, Ie-Bin Lian, Day-Yu Chao
CLINICAL INTERVENTIONS IN AGING
(2020)
Article
Virology
Jedhan U. Galula, Chung-Yu Yang, Brent S. Davis, Gwong-Jen J. Chang, Day-Yu Chao
Article
Multidisciplinary Sciences
Wei-Shan Liang, Yu-Chen He, Hong-Dar Wu, Yao-Tsun Li, Tai-Hwa Shih, Gour-Shenq Kao, Horng-Yuh Guo, Day-Yu Chao
Review
Microbiology
Hui-Ying Ko, Gielenny M. Salem, Gwong-Jen J. Chang, Day-Yu Chao
FRONTIERS IN MICROBIOLOGY
(2020)
Meeting Abstract
Infectious Diseases
Y. -H. Pan, Y. -W. Chien, T. -S. Ho, H. -Y. Ko, S. -F. Chang, C. -Y. Yu, P. -Y. Shu, D. -Y. Chao, C. -C. Ku, C. -C. King
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2020)
Article
Infectious Diseases
Yi-Hua Pan, Mei-Ying Liao, Yu-Wen Chien, Tzong-Shiann Ho, Hui-Ying Ko, Chin-Rur Yang, Shu-Fen Chang, Chia-Yi Yu, Shu-Yu Lin, Pin-Wei Shih, Pei-Yun Shu, Day-Yu Chao, Chao-Ying Pan, Hong-Ming Chen, Guey-Chuen Perng, Chia-Chi Ku, Chwan-Chuen King
Summary: This study conducted a post-outbreak serosurvey in 2016 among older and elderly adults in Kaohsiung and Tainan cities in southern Taiwan to measure past DENV infections. The results showed higher overall seroprevalence with age, district- and city-level differences in anti-DENV IgG antibody prevalence, and similar percentages of secondary DENV infection in both cities. The study highlights the importance of monitoring antibody status and investigating dengue vaccination safety and efficacy in older populations.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Medicine, General & Internal
Jedhan Ucat Galula, Gielenny M. Salem, Raul V. Destura, Roland Remenyi, Day-Yu Chao
Summary: The study compared the diagnostic performances of two NS1-based ELISA methods with an E-based VLP ELISA in determining dengue serostatus, finding that NS1-based methods have comparable accuracies and could effectively assist clinicians in assessing vaccine eligibility.
Article
Multidisciplinary Sciences
Hong-Dar Isaac Wu, Day-Yu Chao
Summary: The study utilized Knox test, scan statistics, and visual tools to investigate the dynamic changes of avian influenza outbreak farms, finding that Knox-based and AGC mapping were more sensitive for small-scale spatio-temporal clustering.
SCIENTIFIC REPORTS
(2021)
Review
Infectious Diseases
Fatima Ericka S. Vista, Ourlad Alzeus G. Tantengco, Micah D. Dispo, Danna Mae S. Opiso, Christian Luke D. C. Badua, John Patrick Z. Gerardo, Juan Raphael M. Perez, Karol Ann T. Baldo, Day-Yu Chao, Leslie Michelle M. Dalmacio
Summary: This review analyzes the trends in seroprevalence studies of flaviviruses using IgG ELISA. The results show that dengue virus is the most studied, while Japanese Encephalitis Virus is the least studied. Geographically, serosurveys align with known disease prevalence. Temporally, the number of serosurveys increases after outbreaks and epidemics, except for JEV which is studied to demonstrate vaccination effectiveness. Commercial kits are commonly used for DENV, WNV, and ZIKV. Overall, most studies use an indirect ELISA format and the choice of antigens varies per virus. Endemicity, cross-reactivities, and kit availabilities affect assay choice in serosurveys.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Virology
Ching-Hung Lin, BoJia Chen, Day-Yu Chao, Feng-Cheng Hsieh, Chun-Chun Yang, Hsuan-Wei Hsu, Hon-Man-Herman Tam, Hung-Yi Wu
Summary: This study investigates the characteristics of defective viral genomes (DVGs) in coronavirus, including their structures, reproducibility, origins, and adaptability to different infection environments. The findings contribute to the understanding of gene expression mechanisms and pathogenesis in coronavirus, and could potentially aid in the development of antiviral strategies.
Meeting Abstract
Biophysics
Jan K. Marzinek, Venkata Raghuvamsi Palur, Gielenny Salem, Fan-Chi Chen, Shang-Rung Wu, Peter J. Bond, Day-Yu Chao
BIOPHYSICAL JOURNAL
(2023)
Meeting Abstract
Biophysics
Venkata Raghuvamsi Palur, Gielenny Salem, Fan-Chi Chen, Shang-Rung Wu, Peter J. Bond, Day-Yu Chao, Jan K. Marzinek
BIOPHYSICAL JOURNAL
(2023)
Article
Immunology
Hong -Dar Isaac Wu, Ruey-Shing Lin, Wen -Han Hwang, Mei-Liang Huang, Bo-Jia Chen, Tseng-Chang Yen, Day -Yu Chao
Summary: The ongoing circulation and reassortment of low-pathogenic avian influenza viruses have caused economic losses and public health concerns. Virologic surveillance of wild birds is recommended as part of a global monitoring system. However, underreporting and biased sampling have hindered understanding of highly pathogenic avian influenza transmission. In this study, we used the eBird database to examine the distribution of wild birds in Taiwan and their potential for virus exchange with domestic poultry. Our findings provide a methodological blueprint for incorporating this into the global surveillance system.
EMERGING INFECTIOUS DISEASES
(2023)
Article
Virology
Yao-Tsun Li, Chen-Chih Chen, Ai-Mei Chang, Day-Yu Chao, Gavin J. D. Smith
Article
Biology
Jedhan Ucat Galula, Gwong-Jen J. Chang, Day-Yu Chao