Article
Cell & Tissue Engineering
Stephen T. Moore, Takashi Nakamura, Jing Nie, Alexander J. Solivais, Isabel Aristizabal-Ramirez, Yoshitomo Ueda, Mayakannan Manikandan, V. Shweta Reddy, Daniel R. Romano, John R. Hoffman, Benjamin J. Perrin, Rick F. Nelson, Gregory I. Frolenkov, Susana M. Chuva de Sousa Lopes, Eri Hashino
Summary: Using 3D cultures of human pluripotent stem cells, researchers replicated the differentiation process of cochlear hair cells by modulating Sonic Hedgehog and WNT signaling. The resulting cells displayed characteristics consistent with both outer and inner hair cells in the cochlea. These findings suggest the development of a promising system to model the human auditory organ.
Article
Biotechnology & Applied Microbiology
Ibrahim C. Kurt, Ronghao Zhou, Sowmya Iyer, Sara P. Garcia, Bret R. Miller, Lukas M. Langner, Julian Grunewald, J. Keith Joung
Summary: This study presents two base editor architectures that can efficiently induce targeted C-to-G base transversions, with reduced levels of unwanted mutations. These new base editors hold promise for optimizing C-to-G base editors for both research and therapeutic applications.
NATURE BIOTECHNOLOGY
(2021)
Review
Cell & Tissue Engineering
Jiangtao Li, Xin Feng, Xiang Wei
Summary: This review summarizes the methods of establishing cardiomyocyte models using induced pluripotent stem cells (iPSCs) and the progress in genome editing techniques. Because the currently cultured iPSC-CMs are immature, researchers have attempted various methods to promote their maturation. Many researchers have established iPSC-CM models of hypertrophic cardiomyopathy (HCM) and used diverse methods for measurements.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Oncology
Cedar Schloo, Lena M. Kutscher
Summary: Pluripotent stem cells are valuable tools in studying human-specific disease and cancer research, especially for brain and neural crest neoplasms. Their ability to reactivate shared transcriptomic and epigenetic signatures provides insights into oncogenic processes and developmental programs. Brain organoids have improved the realism of in vitro models, making them more suitable for biomedical modeling. This review emphasizes the need for rigorous quality control and the development of models for rare tumor subtypes.
Article
Multidisciplinary Sciences
Shireen R. Lamande, Elizabeth S. Ng, Trevor L. Cameron, Louise H. W. Kung, Lisa Sampurno, Lynn Rowley, Jinia Lilianty, Yudha Nur Patria, Tayla Stenta, Eric Hanssen, Katrina M. Bell, Ritika Saxena, Kathryn S. Stok, Edouard G. Stanley, Andrew G. Elefanty, John F. Bateman
Summary: Researchers have developed a method to direct iPSC-derived sclerotome to differentiate into chondroprogenitors or articular chondrocytes, and further transition into hypertrophic chondrocytes and osteoblasts. The system can be used to model genetic skeletal disorders and generate cells for regenerative medicine applications, mimicking in vivo bone formation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Cell Biology
Mario G. Pavez-Giani, Lukas Cyganek
Summary: This review summarizes recent advances in iPSC-based disease modeling of mitochondrial cardiomyopathies and explores the patho-mechanistic insights as well as new therapeutic approaches. Researchers have used iPSC technology to recapitulate major characteristics of mitochondrial cardiomyopathies, providing a powerful platform for drug development and testing of new therapies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Oncology
Ryusaku Matsumoto, Hidetaka Suga, Hiroshi Arima, Takuya Yamamoto
Summary: The lack of human pituitary cell lines has limited the study of the molecular mechanisms of pituitary tumors. Recent research has successfully induced pituitary cells from human-induced pluripotent stem cells (hiPSCs), providing an opportunity to develop in vitro models of human pituitary adenomas. These models have great potential for discovering new drugs and investigating the development and pathophysiology of pituitary tumors.
Article
Developmental Biology
Robert Morey, Tony Bui, Kathleen M. Fisch, Mariko Horii
Summary: This article describes in vitro models of human trophoblast differentiation, including primary and hPSC-derived TSC as well as trophoblast organoids. These models help study early placental development and diseases in various settings.
Article
Multidisciplinary Sciences
Gou Takahashi, Daiki Kondo, Minato Maeda, Yuji Morishita, Yuichiro Miyaoka
Summary: This study utilized the Single Particle isolation System to analyze genome editing outcomes in individual human cultured cells, revealing a binary nature of genome editing induction and providing a new strategy for analyzing genome editing outcomes in single cells.
Article
Multidisciplinary Sciences
Yu Jin Jang, Mijeong Kim, Bum-Kyu Lee, Jonghwan Kim
Summary: The authors developed a simple procedure to convert human primed pluripotent stem cells into trophoblast stem-like cells using bone morphogenetic protein 4, providing an important opportunity to study human placenta development and pathology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Glyn Nigel Stacey, Jie Hao
Summary: In recent years, significant progress has been made in China in establishing hPSCs for the manufacture of cell therapies. The National Stem Cell Resource Center and its partner organizations have over 600 hPSC lines formally recorded, with 47 of them also registered for European Commission funded research projects. This national and international coordination aims to promote the translation of Chinese hPSC-based products into clinical use according to established standards.
CELL PROLIFERATION
(2022)
Review
Biochemistry & Molecular Biology
Michael Dannemann, Irene Gallego Romero
Summary: The emergence of induced pluripotent stem cells (iPSCs) has transformed the study of human evolution by providing a viable and powerful model system. With the ability to be established easily, maintained indefinitely, and differentiated into various tissue types, iPSCs are rapidly becoming a crucial tool in addressing previously challenging questions in human evolution.
Review
Biochemistry & Molecular Biology
Baiyan Ren, Anna Dunaevsky
Summary: Astrocytes, a subtype of glial cells in the central nervous system, exhibit morphological and functional diversity; human pluripotent stem cell differentiation approaches play an important role in studying the etiology of neurological disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pediatrics
Weibo Niu, Benjamin Siciliano, Zhexing Wen
Summary: This review provides an overview of the recent progress in modeling tuberous sclerosis complex (TSC) using human-induced pluripotent stem cells (iPSCs), highlighting the importance of human-specific models in studying TSC.
WORLD JOURNAL OF PEDIATRICS
(2022)
Review
Cell Biology
Fabian Oceguera-Yanez, Alfonso Avila-Robinson, Knut Woltjen
Summary: This article provides an overview of strategies for differentiating human pluripotent stem cells (PSCs) towards skin components, with a particular focus on keratinocytes. The use of patient-derived human induced PSCs (iPSCs) and skin organoid generation allows researchers to model inherited skin diseases and search for potential treatments.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alexander Smirnov, Veniamin Fishman, Anastasia Yunusova, Alexey Korablev, Irina Serova, Boris Skryabin, Timofey S. Rozhdestvensky, Nariman Battulin
NUCLEIC ACIDS RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Nataliya Gorinski, Daniel Wojciechowski, Daria Guseva, Dalia Abdel Galil, Franziska E. Mueller, Alexander Wirth, Stefan Thiemann, Andre Zeug, Silke Schmidt, Monika Zareba-Koziol, Jakub Wlodarczyk, Boris Skryabin, Silke Glage, Martin Fischer, Samer Al-Samir, Nicole Kerkenberg, Christa Hohoff, Weiqi Zhang, Volker Endeward, Evgeni Ponimaskin
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Immunology
Toni Weinhage, Timo Wirth, Paula Schuetz, Philipp Becker, Aloys Lueken, Boris Skryabin, Helmut Wittkowski, Dirk Foell
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Cell Biology
Dingding Mo, Xinping Li, Carsten A. Raabe, Timofey S. Rozhdestvensky, Boris V. Skryabin, Juergen Brosius
Article
Medicine, Research & Experimental
Konstanze Michel, Melissa Herwig, Franziska Werner, Katarina Spiranec Spes, Marco Abesser, Kai Schuh, Swati Dabral, Andreas Muegge, Hideo A. Baba, Boris V. Skryabin, Nazha Hamdani, Michaela Kuhn
Review
Cardiac & Cardiovascular Systems
Ralf P. Brandes, Anne Dueck, Stefan Engelhardt, Manuel Kaulich, Christian Kupatt, Maria Teresa De Angelis, Matthias S. Leisegang, Ferdinand le Noble, Alessandra Moretti, Oliver J. Mueller, Boris V. Skryabin, Thomas Thum, Wolfgang Wurst
Summary: Gene editing has evolved from a scientific concept to a widely used technology in laboratories, with revolutionary implications for basic science and clinical treatment. Researchers are striving to advance the field by developing new techniques and combining cellular and animal applications to address challenges in gene editing.
BASIC RESEARCH IN CARDIOLOGY
(2021)
Article
Urology & Nephrology
Carolin Lepa, Sascha Hoppe, Antje Stoeber, Boris V. Skryabin, Laura Katharina Sievers, Barbara Heitplatz, Giuliano Ciarimboli, Ute Neugebauer, Maja T. Lindenmeyer, Clemens D. Cohen, Hannes C. A. Drexler, Peter Boor, Thomas Weide, Hermann Pavenstaedt, Britta George
Summary: The research results indicate that TrkC is crucial for maintaining glomerular integrity and modulating Igf-related signaling in podocytes. This has significant implications for the development of targeted therapies for glomerular diseases.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Review
Biochemistry & Molecular Biology
Delf-Magnus Kummerfeld, Carsten A. Raabe, Juergen Brosius, Dingding Mo, Boris Skryabin, Timofey S. Rozhdestvensky
Summary: PWS is a neurogenetic disorder caused by the deletion of paternally imprinted genes on chromosome 15q11-q13, with mouse models playing a crucial role in understanding the syndrome's pathogenesis. Through murine models, the contribution of each affected gene to PWS has been unveiled, shedding light on the importance of non-protein coding genes in the locus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Sanaa Eddiry, Gwenaelle Diene, Catherine Molinas, Juliette Salles, Francoise Conte Auriol, Isabelle Gennero, Eric Bieth, Boris Skryabin, Timofey S. Rozhdestvensky, Lisa C. Burnett, Rudolph L. Leibel, Maithe Tauber, Jean Pierre Salles
Summary: The study found elevated levels of IGFBP7 in PWS patients, which normalized under growth hormone therapy. Elevated levels of IGFBP7 were also found in Snord116 knockout mice and iPSC-derived neurons from SNORD116-deleted PWS patients. The findings suggest that SNORD116 deletion affects IGFBP7 levels and that modulation of IGFBP7 levels has implications in the management of PWS under growth hormone therapy.
GENETICS IN MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Nadja Bork, Anna Kuret, Melanie Cruz Santos, Cristina E. Molina, Beate Reiter, Hermann Reichenspurner, Andreas Friebe, Boris Skryabin, Timofey S. Rozhdestvensky, Michaela Kuhn, Robert Lukowski, Viacheslav O. Nikolaev
Summary: This study reveals that hypoxia triggers rapid degradation of PDE3A protein via the autophagosomal-lysosomal pathway, leading to increased cGMP levels that prevent cell death and enhance protective effects of NO-GC activators.
Article
Biochemistry & Molecular Biology
Delf-Magnus Kummerfeld, Boris Skryabin, Juergen Brosius, Sergey Y. Vakhrushev, Timofey S. Rozhdestvensky
Summary: Prader-Willi syndrome is a complex neurodevelopmental disorder associated with the deletion or inactivation of specific genes. This study identified stably expressed reference genes in different brain regions of mice, and found significant differences in the expression patterns of certain genes between wild-type and Prader-Willi syndrome mice. These findings provide valuable insights into the regulatory pathways involved in this disorder.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Aliska K. Brugmans, Carolin Walter, Natalia Moreno, Carolin Goebel, Doerthe Holdhof, Flavia W. de Faria, Marc Hotfilder, Daniela Jeising, Michael C. Fruehwald, Boris V. Skryabin, Timofey S. Rozhdestvensky, Lydia Wachsmuth, Cornelius Faber, Martin Dugas, Julian Varghese, Ulrich Schueller, Thomas K. Albert, Kornelius Kerl
Summary: Researchers have established a new mouse model to study the impact of specific non-truncating mutations in the Smarcb1 gene on brain development. Using magnetic resonance imaging, histology, and single-cell RNA sequencing, they found that these mutations disrupt neuronal development in newborn mice, affecting their weight gain and leading to enlarged brain ventricles.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2023)
Article
Cell Biology
Egor B. Skryabin, Kirstie A. De Jong, Hariharan Subramanian, Nadja I. Bork, Alexander Froese, Boris V. Skryabin, Viacheslav O. Nikolaev
Summary: This study successfully utilized the CRISPR/Cas9 approach to knock out PDE2A and PDE3A genes and their isoforms in neonatal and adult rat cardiomyocytes, revealing distinct roles of these isoforms in regulating cAMP dynamics in different developmental stages.
Meeting Abstract
Biochemistry & Molecular Biology
T. S. Rozhdestvensky, D. Kummerfeld, J. Roth, S. G. Meuth, H. Pavenstaedt, J. Sherwood, T. Pap, R. Wedlich-Soeldner, C. Sunderkoetter, Y. B. Schwartz, J. Brosius, B. V. Skryabin
Article
Physiology
Vladimir V. Matchkov, Henrik Black Joergensen, Dmitrii Kamaev, Andreas Hoegh Jensen, Hans Christian Beck, Boris Skryabin, Christian Aalkjaer
PHYSIOLOGICAL REPORTS
(2020)