Article
Chemistry, Multidisciplinary
Yu-An Kuo, Cheulhee Jung, Yu-An Chen, Hung-Che Kuo, Oliver S. Zhao, Trung D. Nguyen, James R. Rybarski, Soonwoo Hong, Yuan- Chen, Dennis C. Wylie, John A. Hawkins, Jada N. Walker, Samuel W. J. Shields, Jennifer S. Brodbelt, Jeffrey T. Petty, Ilya J. Finkelstein, Hsin-Chih Yeh
Summary: The study revealed that the nucleobases at positions 7-12 of the 18-nucleotide-long activator are critical to creating bright NCBs, while positions 4-6 and 2-4 are hotspots to generate yellow-orange and red POTs.
ADVANCED MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Yongping Li, Tobias Fehlmann, Adam Borcherding, Snezana Drmanac, Sophie Liu, Laura Groeger, Chongjun Xu, Matthew Callow, Christian Villarosa, Alexander Jorjorian, Fabian Kern, Nadja Grammes, Eckart Meese, Hui Jiang, Radoje Drmanac, Nicole Ludwig, Andreas Keller
Summary: Results show that CoolMPS, a new sequencing chemistry, outperformed standard sequencing-by-synthesis in terms of sequencing quality, read mapping percentage, and discovery of novel miRNAs. There was a high correlation between CoolMPS and standard methods, indicating the reliability of CoolMPS for non-coding RNA analysis.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemical Research Methods
James Gooch, Sireethorn Tungsirisurp, Hayley Costanzo, Richard Napier, Nunzianda Frascione
Summary: The study selected ssDNA aptamers against human sperm cells using Cell-SELEX and massively parallel sequencing technologies, revealing promising aptamer candidates that selectively bind sperm cells. These aptamers have the potential to increase the efficiency of sexual offence investigations by facilitating sperm detection.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Review
Endocrinology & Metabolism
Aideen M. McInerney-Leo, Emma L. Duncan
Summary: There have been two major eras in gene discovery history, from linkage analysis to massively parallel sequencing (MPS). MPS has greatly accelerated the identification of disease genes, reducing the time needed to determine disease genes from years to weeks.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Genetics & Heredity
Erin M. Gorden, Kimberly Sturk-Andreaggi, Charla Marshall
Summary: Hybridization capture technology has gained attention in the forensic field for its ability to process degraded DNA fragments and enrich mitochondrial DNA or nuclear genome polymorphisms through massively parallel sequencing. As the technology advances, hybridization capture becomes increasingly important in analyzing DNA from aged and degraded remains.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2021)
Review
Chemistry, Multidisciplinary
Junyun Cheng, Gaole Lin, Tianhao Wang, Yunzhu Wang, Wenbo Guo, Jie Liao, Penghui Yang, Jie Chen, Xin Shao, Xiaoyan Lu, Ling Zhu, Yi Wang, Xiaohui Fan
Summary: CRISPR-based genetic screening is a powerful approach for unbiased functional genomics research. Single-cell CRISPR screening techniques allow the analysis of regulatory networks in complex biological systems at unprecedented resolution. These methods involve perturbing cells en masse using a pooled CRISPR library and high-content phenotyping.
Article
Obstetrics & Gynecology
Songchang Chen, Xuyang Yin, Sijia Zhang, Jun Xia, Ping Liu, Pingyuan Xie, Huijuan Yan, Xinming Liang, Junyu Zhang, Yiyao Chen, Hongjun Fei, Lanlan Zhang, Yuting Hu, Hui Jiang, Ge Lin, Fang Chen, Chenming Xu
Summary: WGS offers a cost-effective and reliable approach for genome-wide haplotyping and PGT for monogenic disorders, aneuploidy, and structural rearrangements without the need for additional family members, showing 100% concordance with traditional PGT methods.
HUMAN REPRODUCTION
(2021)
Article
Microbiology
Wataru Aoki, Masato Kogawa, Shuhei Matsuda, Keisuke Matsubara, Shintaro Hirata, Yohei Nishikawa, Masahito Hosokawa, Haruko Takeyama, Toru Matoh, Mitsuyoshi Ueda
Summary: This study developed a pipeline to analyze the genomic diversity of the rice rhizosphere microbiome at single-cell resolution. By isolating microbial cells from paddy soil and determining their genomic sequences using massively parallel whole-genome amplification, the researchers obtained a large number of genomic sequences that provided insights into microbial functions in the paddy ecosystem. This approach offers a promising platform for gaining novel insights into the roles of microbes in the rice rhizomicrobiome and developing microbial technologies for improved and sustainable rice production.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ludmila Volozonoka, Anna Miskova, Linda Gailite
Summary: This review provides insight into the performance and drawbacks of WGA techniques such as DOP-PCR, MDA, MALBAC, and hybrid techniques in PGT. The advantages of MDA-based solutions for comprehensive genome coverage and PCR-based solutions for uniformity of coverage are highlighted. The choice of WGA technique should take into consideration the influence of MPS parameters.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Sanna Loppi, Paula Korhonen, Maria Bouvy-Liivrand, Simone Caligola, Tiia A. Turunen, Mikko P. Turunen, Ana Hernandez de Sande, Natalia Kolosowska, Flavia Scoyni, Anna Rosell, Teresa Garcia-Berrocoso, Sighild Lemarchant, Hiramani Dhungana, Joan Montaner, Jari Koistinaho, Katja M. Kanninen, Minna U. Kaikkonen, Rosalba Giugno, Merja Heinaniemi, Tarja Malm
Summary: Ischemic stroke, the third leading cause of death in the Western world, mainly affects the elderly and is exacerbated by comorbid conditions such as atherosclerosis or diabetes. Research shows that acute peripheral inflammation worsens stroke-induced neuronal damage in aged mice, linked to increased levels of plasma proinflammatory cytokines. Targeting molecular pathways involved in this process may help improve outcomes for elderly stroke patients.
Article
Cell Biology
Paola Sebastiani, Anthony Federico, Melody Morris, Anastasia Gurinovich, Toshiko Tanaka, Kevin B. Chandler, Stacy L. Andersen, Gerald Denis, Katherine Costello, Luigi Ferrucci, Lori Jennings, David J. Glass, Stefano Monti, Thomas T. Perls
Summary: Using samples from the New England Centenarian Study, researchers characterized the serum proteome of centenarians, their offspring, and age-matched controls. They identified protein signatures associated with longer survival in centenarians and younger individuals and found similarities in aging markers between centenarians and short-lived cohorts. The study provides specific circulating biomarkers of healthy aging and longevity, suggesting potential mechanisms to prolong health and support longevity.
Article
Multidisciplinary Sciences
Libia Alejandra Garcia-Flores, Cara L. Green, Sharon E. Mitchell, Daniel E. L. Promislow, David Lusseau, Alex Douglas, John R. Speakman
Summary: Caloric restriction affects the metabolome in a tissue-specific manner, with only a small number of common metabolic features responding in the same way across all tissues. The top modulated pathways were related to biosynthesis and degradation processes, suggesting each tissue has its own unique way of coping with reduced energy intake, supporting the clean cupboards hypothesis over the disposable soma interpretation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Genetics & Heredity
Cordula Haas, Jacqueline Neubauer, Andrea Patrizia Salzmann, Erin Hanson, Jack Ballantyne
Summary: Transcriptome analyses have seen significant growth in forensic genetics in recent years, with applications such as body fluid and tissue identification, determining stain and donor age, estimating post-mortem interval, and conducting post mortem death investigations. The introduction of Massively Parallel Sequencing (MPS) has opened up new possibilities for this field, allowing for high-resolution analyses and whole transcriptome sequencing of forensically relevant materials.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2021)
Article
Geriatrics & Gerontology
Elena Sabini, Alison O'Mahony, Patrizio Caturegli
Summary: This study compares the therapeutic effects of two drugs, MyMD-1 and rapamycin, on aging and age-related diseases. The results demonstrate that MyMD-1 has better effects on lifespan and health span characteristics in mice, and exhibits stronger anti-proliferative, anti-inflammatory, and anti-fibrotic properties in vitro.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2023)
Article
Genetics & Heredity
Ruiyang Tao, Qiannan Xu, Shouyu Wang, Ruocheng Xia, Qi Yang, Anqi Chen, Yiling Qu, Yehui Lv, Suhua Zhang, Chengtao Li
Summary: With the development of massively parallel sequencing (MPS), the kinship analysis has become more convenient. In this study, 243 Chinese Han individuals from 17 families were sequenced using the ForenSeq Signature Kit, and the analysis was conducted using likelihood ratio and identical by state methods. The results showed that the kit was effective in analyzing parent-child, full sibling, and most second-degree kinships.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2022)