Article
Immunology
Jing Gao, Qiujing Chen, Yiqian Peng, Nanyan Jiang, Youhao Shi, Chunmei Ying
Summary: The study aimed to explore the potential clinical application of the Copan WASP automated preanalytical microbiology processing system in detecting pathogens in female reproductive tract specimens, finding that the automated system had higher efficiency in isolating single colonies compared to manual methods, but detected fewer bacterial types and colonies.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Abdessalam Cherkaoui, Gesuele Renzi, Nicolas Vuilleumier, Jacques Schrenzel
Summary: The study assessed the agreement at the categorical level between the Vitek 2 system and the Colibri coupled to the Radian under real laboratory conditions, with high overall categorical agreements. However, Vitek 2 had significant errors in P. aeruginosa testing. The Colibri coupled to the Radian provides an automated solution for antimicrobial disk diffusion susceptibility testing with accuracy equal to or better than the Vitek 2 system.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Infectious Diseases
Olivier Dauwalder, Agathe Michel, Cecile Eymard, Kevin Santos, Laura Chanel, Anatole Luzzati, Pablo Roy-Azcora, Jean Francois Sauzon, Marc Guillaumont, Pascale Girardo, Christine Fuhrmann, Gerard Lina, Frederic Laurent, Francois Vandenesch, Chantal Sobas
Summary: The study aimed to validate a workflow designed using artificial intelligence software PhenoMATRIX (PHM), with results showing over 90% agreement with LT results, potentially saving laboratory technologists' time.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Immunology
Mihir Kekre, Stefany Alejandra Arevalo, Maria Fernanda Valencia, Marietta L. Lagrada, Polle Krystle Macaranas, Geetha Nagaraj, Anderson O. Oaikhena, Agnettah M. Olorosa, David M. Aanensen
Summary: Antimicrobial resistance (AMR) is a global threat, and incorporating whole-genome sequencing (WGS) can enhance phenotypic data in epidemiological surveillance. Despite the high initial investment required for laboratory infrastructure and maintenance, on-boarding WGS capabilities can greatly improve real-time processing power within regional and national AMR surveillance initiatives.
CLINICAL INFECTIOUS DISEASES
(2021)
Review
Immunology
Valery Daubie, Houssein Chalhoub, Bob Blasdel, Hafid Dahma, Maya Merabishvili, Tea Glonti, Nathalie De Vos, Johan Quintens, Jean-Paul Pirnay, Marie Hallin, Olivier Vandenberg
Summary: As the global burden of disease caused by multidrug resistant bacteria is a major concern, exploring credible clinical alternatives to antibiotic therapy, such as personalized phage therapy, is crucial. However, the development of an easy to implement diagnostic tool for determining phage susceptibility remains an open issue. This review summarizes existing methods for determining phage activity on bacteria and discusses potential techniques for implementing a phage susceptibility testing tool in routine clinical microbiology.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Naoki Watanabe, Yoshihito Otsuka, Tomohisa Watari, Naoto Hosokawa, Kazufumi Yamagata, Miyuki Fujioka
Summary: The study evaluated the usefulness of time to positivity (TTP) in diagnosing true bacteremia and contamination in cases where Corynebacterium was isolated from blood samples. The results showed that TTP had a certain sensitivity and specificity for diagnosing true bacteremia, especially in cases with TTP <= 69.4 hours.
Article
Chemistry, Multidisciplinary
Shao-Hsuan Kuo, Alberto Peraro, Hsiu-Pen Lin, Chun-Hao Chang, Bor -Ran Li
Summary: Urinalysis is a simple and common medical test in modern cities, but it remains a challenge for residents in healthcare-poor areas. Therefore, developing a smart and affordable widget to address this challenge is crucial. The proposed mu CUREX platform successfully provides an ultra-low-cost solution for urinalysis in resource-poor settings.
Article
Multidisciplinary Sciences
Aurelie Morand, Mamadou Lamine Tall, Edmond Kuete Yimagou, Issa Isaac Ngom, Cheikh Ibrahima Lo, Florent Cornu, Michel Tsimaratos, Jean-Christophe Lagier, Anthony Levasseur, Didier Raoult, Pierre-Edouard Fournier
Summary: This study describes a new species Anaerococcus urinomassiliensis, isolated from the urine of a 17-year-old boy with autoimmune hepatitis and membranoproliferative glomerulonephritis. The strain exhibits specific genetic characteristics, is primarily of human origin, and belongs to the genus of anaerobic bacteria.
SCIENTIFIC REPORTS
(2021)
Review
Infectious Diseases
Cherry Lim, Elizabeth A. Ashley, Raph L. Hamers, Paul Turner, Thomas Kesteman, Samuel Akech, Alejandra Corso, Mayfong Mayxay, Iruka N. Okeke, Direk Limmathurotsakul, H. Rogier van Doorn
Summary: This article reviews the strategies for AMR surveillance using routine microbiology results in LMICs, emphasizing the need for support in certain areas. Different surveillance strategies need to be thoroughly understood in terms of their limitations and potential biases to ensure maximum utilization and interpretation of local routine microbiology data.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Infectious Diseases
Yuliya Zboromyrska, Veronica Rico, Cristina Pitart, Mariana Jose Fernandez-Pittol, Alex Soriano, Jordi Bosch
Summary: The direct identification and rapid detection of ESBL and carbapenemase-producing strains from urine samples is a reliable and useful tool in adjusting empirical antimicrobial treatment.
Article
Microbiology
Sylvie Buffet-Bataillon, Guillaume Rizk, Vincent Cattoir, Mohamed Sassi, Vincent Thibault, Jennifer Del Giudice, Jean-Pierre Gangneux
Summary: Metagenomics analysis is widely used for clinical diagnosis, but we need confidence in interpreting the results. In this study, we propose a methodology for taxon identification and abundance assessment of microbiota data, with a focus on quality control and the introduction of an external positive control. The use of multiple complementary classifiers greatly improves the reliability of the analysis results.
Article
Pediatrics
Neaha Patel, Lisa NeoTRIPS Collaborative Group, Katie Evans, Janet Berrington, Lisa Szatkowski, Kate Costeloe, Shalini Ojha, Paul Fleming, Cheryl Battersby
Summary: A survey conducted in neonatal units in the UK found that over 40% of the units are routinely using probiotics, but there is variability in the products used. Further research is needed to determine if the use of probiotics can lead to improved clinical outcomes.
BMJ PAEDIATRICS OPEN
(2023)
Article
Multidisciplinary Sciences
Rafael Carvalho da Silva, Joao Marcelo Robazzi Bignelli Valente Aguiar, Cintia Akemi Oi, Jaqueline Eterna Batista, Martin Gurfa, Fabio Santos do Nascimento
Summary: Despite their role as pollinators and predators, the cognitive capacities of wasps, such as learning and memory, have been largely unexplored. In this study, we used Pavlovian conditioning to show that the neotropical wasp Mischocyttarus cerberus efficiently learns and remembers odor-sugar associations. However, differences in learning ability were observed between male and female wasps, possibly due to their different lifestyles. These findings highlight the importance of socioecological constraints on wasp cognition and provide a basis for further mechanistic studies on learning differences across ages and castes.
Article
Infectious Diseases
Zeynep Ceren Karahan, Irem Altinsoy, Bilge Nur Caliskan, Sila Dede, Gorkem Kayis, Hasan Can Turkoglu, Ebru Evren, Beyza Doganay Erdogan, Selin Gamze Kilic, Istar Dolapci, Alper Tekeli
Summary: This research investigated the incidence and traits of capnophilic Escherichia coli (CEC) strains causing urinary tract infections (UTIs). Among 8500 urine samples, only nine (0.11%) epidemiologically unrelated CEC isolates with varying antibiotic susceptibility patterns were identified. Three of these isolates belonged to the O25b-ST131 clone, but none of them possessed the yadF gene. Isolation of CEC is difficult due to adverse incubation conditions, and capnophilic incubation of urine cultures may be considered for patients with underlying predisposing conditions.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Review
Medicine, General & Internal
Meilu Liu, Guilan Cheng, Chan Xiong, Wei Xiao, Long-yi Du, Bing Mao, Yu Li, Ti-wei Miao, Juan-Juan Fu
Summary: This study aims to conduct a systematic review and meta-analysis of the diagnostic performance of mycological tests for invasive pulmonary aspergillosis (IPA) in non-haematological diseases. Database search, data extraction, and statistical analysis will be conducted to evaluate the sensitivity, specificity, and other diagnostic indicators. Subgroup analysis and sensitivity analysis will be performed to explore potential sources of heterogeneity and factors influencing the diagnostic effects of the tests.
Letter
Infectious Diseases
Elias Bodendoerfer, Peter M. Keller, Stefano Mancini
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2019)
Article
Public, Environmental & Occupational Health
B. Hasse, M. M. Hannan, P. M. Keller, F. P. Maurer, R. Sommerstein, D. Mertz, D. Wagner, N. Fernandez-Hidalgo, J. Nomura, V Manfrin, D. Bettex, A. Hernandez Conte, E. Durante-Mangoni, T. H-C Tang, R. L. Stuart, J. Lundgren, S. Gordon, M. C. Jarashow, P. W. Schreiber, S. Niemann, T. A. Kohl, C. L. Daley, A. J. Stewardson, C. J. Whitener, K. Perkins, D. Plachouras, T. Lamagni, M. Chand, T. Freiberger, S. Zweifel, P. Sander, B. Schulthess, J. E. Scriven, H. Sax, J. van Ingen, C. A. Mestres, D. Diekema, B. A. Brown-Elliott, R. J. Wallace, L. M. Baddour, J. M. Miro, B. Hoen, E. Athan, A. Bayer, B. Barsic, G. R. Corey, V. H. Chu, D. T. Durack, C. Querido Fortes, V Fowler, A. Waller Krachmer, E. Durante-Magnoni, M. Miro, W. R. Wilson, J. Striven, R. Stewart, T. Hing-Cheung Tang, L. A. Herwaldt, P. Schreiber, A. Stewardson, A. Widmer, B. A. Brown Elliot, C. Daley, P. Keller, F. Maurer, V Falk, M. Halbe, K. M. Perkins
JOURNAL OF HOSPITAL INFECTION
(2020)
Article
Infectious Diseases
Benjamin Preiswerk, Frank Imkamp, Denise Vorburger, Rico Hoemke, Peter M. Keller, Karoline Wagner
BMC INFECTIOUS DISEASES
(2020)
Article
Microbiology
Edgar Campos-Madueno, Odette J. Bernasconi, Aline Moser, Peter M. Keller, Francesco Luzzaro, Carola Maffioli, Thomas Bodmer, Andreas Kronenberg, Andrea Endimiani
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2020)
Editorial Material
Infectious Diseases
Peter M. Keller, Hansjakob Furrer
LANCET INFECTIOUS DISEASES
(2020)
Article
Microbiology
Martina L. Reichmuth, Rico Homke, Kathrin Zurcher, Peter Sander, Anchalee Avihingsanon, Jimena Collantes, Chloe Loiseau, Sonia Borrell, Miriam Reinhard, Robert J. Wilkinson, Marcel Yotebieng, Lukas Fenner, Erik C. Bottger, Sebastien Gagneux, Matthias Egger, Peter M. Keller
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2020)
Article
Immunology
Sarah Flohr, Alban Ramette, Philipp K. A. Agyeman, Andrea Duppenthaler, Cordula Scherer, Peter M. Keller, Christoph Aebi
OPEN FORUM INFECTIOUS DISEASES
(2020)
Article
Microbiology
Giovanni Ghielmetti, Giuliana Rosato, Alberto Trovato, Ute Friedel, Constanze Kirchgaessner, Carmen Perroulaz, Wolfgang Pendl, Bettina Schulthess, Guido V. Bloemberg, Peter M. Keller, Roger Stephan, Enrico Tortoli
Summary: The study identified a novel slow-growing mycobacterial species isolated from lymph nodes of two sows in Switzerland, which were not related to any previously described Mycobacterium species. The new species proposed was named Mycobacterium helveticum sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Immunology
Konrad Egli, Karoline Wagner, Peter M. Keller, Lorenz Risch, Martin Risch, Thomas Bodmer
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2020)
Article
Microbiology
David Dylus, Trestan Pillonel, Onya Opota, Daniel Wuethrich, Helena M. B. Seth-Smith, Adrian Egli, Stefano Leo, Vladimir Lazarevic, Jacques Schrenzel, Sacha Laurent, Claire Bertelli, Dominique S. Blanc, Stefan Neuenschwander, Alban Ramette, Laurent Falquet, Frank Imkamp, Peter M. Keller, Andre Kahles, Simone Oberhaensli, Valerie Barbie, Christophe Dessimoz, Gilbert Greub, Aitana Lebrand
FRONTIERS IN MICROBIOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Michele Sorgenfrei, Lea M. Hurlimann, Melissa M. Remy, Peter M. Keller, Markus A. Seeger
Summary: This article summarizes various biomolecules used for capturing live bacteria, including immune proteins, antibodies, aptamers, phage proteins, and antimicrobial peptides. The article also discusses the potential use of nanobodies targeting conserved surface-accessible proteins as promising biomolecules for pathogen capture.
TRENDS IN BIOCHEMICAL SCIENCES
(2022)
Article
Medicine, General & Internal
Martin Mueller, Nadine Saegesser, Peter M. Keller, Spyridon Arampatzis, Benedict Steffens, Simone Ehrhard, Alexander B. Leichtle
Summary: UFC parameters can safely rule out significant bacterial growth, but cannot adequately predict positive urine culture growth or mixed culture growth.
Article
Biochemistry & Molecular Biology
Michael P. Bronnimann, Lea Hirzberger, Peter M. Keller, Monika Gsell-Albert
Summary: This study examined the antibacterial effect and mechanism of action of iodinated X-ray contrast agents and complexed lanthanide MRI contrast solutions on six different microorganisms. Bacteria were exposed to media containing contrasting agents at different concentrations and pH levels for varying durations. The antibacterial effect was further tested using agar disk diffusion analysis and the microdilution inhibition method. Bactericidal effects were observed at low concentrations and low pH, specifically for Staphylococcus aureus and Escherichia coli.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Aline Moser, Edgar Campos-Madueno, Parham Sendi, Vincent Perreten, Peter M. Keller, Alban Ramette, Andrea Endimiani
Summary: This study characterized an OXA-484 carbapenemase-producing Escherichia coli strain from a Swiss patient infected by SARS-CoV-2 and repatriated from India. The patient was found to be infected with the SARS-CoV-2 B.1.617.2 lineage and colonized with a sequence type 410 E. coli strain producing OXA-484, a carbapenemase not yet reported in Switzerland.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Meeting Abstract
Cardiac & Cardiovascular Systems
S. Barcikowski, P. M. Keller, C. Benden, N. J. Mueller
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2020)