Article
Biochemistry & Molecular Biology
Mariano Elean, Leonardo Albarracin, Julio Villena, Haruki Kitazawa, Lucila Saavedra, Elvira M. Hebert
Summary: A comprehensive genomic analysis of L. delbrueckii strains was conducted, focusing on the proteolytic system and the key enzyme, cell envelope-associated proteinase (CEP). The study compared amino acid sequences and protein structures of CEPs, as well as analyzed syntenic and genomic variations among different strains. Investigation of CEP promoter region and regulatory elements provided insights into the control of proteolytic activity in lactobacilli. This study contributes to our understanding of proteolytic activity and potential applications of L. delbrueckii in the dairy industry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Zihang Shi, Xiankang Fan, Maolin Tu, Zhen Wu, Daodong Pan
Summary: The study found that different strains of Lactobacillus and Lactococcus have varying protein hydrolysis capacities, resulting in significant differences in characteristics such as protein hydrolysis, pH, acidity, viable bacteria count, water holding capacity, and texture of fermented milk. Metabolomics also identified differences in flavor substances between the two strains. L. helveticus was found to improve the sensory quality of fermented milk compared to L. lactis. This study provides new insights into selecting suitable strains for fermented milk production from a genetic and metabolic perspective.
Review
Biochemistry & Molecular Biology
Marek Kieliszek, Katarzyna Pobiega, Kamil Piwowarek, Anna M. Kot
Summary: In recent decades, there has been rapid development in the biotechnological use of lactic acid bacteria (LAB) in the food industry. Their proteolytic enzymes play a crucial role in providing cells with nitrogen compounds for growth, and advancements in enzymology allow for the creation of new enzymes with various applications.
Article
Food Science & Technology
Blandine M. L. Genet, Hang Xiao, Lise Friis Christensen, Ida Nynne Laforce, Mohammad Amin Mohammadifar, Claus Heiner Bang-Berthelsen, Egon Bech Hansen
Summary: This study investigated strains isolated from different environments in Denmark for their ability to produce curd from fermentation of Soy Based Milk Alternative (SBMA). Acidification dynamics and metabolite production were analyzed in commercial SBMA, and proteomics were used to determine the hydrolysis profile of soy proteins. Certain strains showed proteolytic activity and were considered potential candidates for producing a soy-based yogurt-like product.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Per Johansson, Elina Sade, Jenni Hultman, Petri Auvinen, Johanna Bjorkroth
Summary: Genomic analysis of 37 strains of Leuconostoc gelidum and Leuconostoc gasicomitatum supports the reclassification of L. gelidum subsp. gasicomitatum to L. gasicomitatum at the species level. The study also revealed limited intraspecies variation in genome properties, which were not attributed to the source of isolation. Additionally, none of the strains carried acquired antimicrobial resistance genes or genes associated with virulence, indicating the lack of functional and phylogenetic diversification driving their distribution and lifestyle in foods.
Article
Biotechnology & Applied Microbiology
Pradip Behare, Syed Azmal Ali, Vinay S. N. Mishra, Laura G. Gomez-Mascaraque, Olivia McAuliffe
Summary: In this study, the viability and morphology of different bacterial strains were examined under high fructose concentrations. It was found that fructophilic lactic acid bacteria had reduced viability, while a non-fructophilic strain was significantly affected. Electron microscopy revealed that fructophilic strains remained intact but expanded, while the non-fructophilic strain ruptured and formed cell debris. Genomic analysis showed that fructophilic strains had fewer genes related to fructose metabolism and transport.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Microbiology
Komwit Surachat, Duangporn Kantachote, Monwadee Wonglapsuwan, Arnon Chukamnerd, Panchalika Deachamag, Pimonsri Mittraparp-arthorn, Kongpop Jeenkeawpiam
Summary: This study investigates the genomic diversity and gene functions of Weissella species in the LAB genus, revealing a high level of genomic diversity and the possible existence of three new species. The genome of Weissella cibaria strain NH9449 encodes several beneficial genes with potential applications in biotechnology.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Shahram Loghman, Ali Moayedi, Mandana Mahmoudi, Morteza Khomeiri, Laura G. Gomez-Mascaraque, Farhad Garavand
Summary: In this study, different strains of lactic acid bacteria were used to manufacture functional fermented milks. The fermented milks showed increased proteolytic, ACE inhibitory, and antioxidant activities, which were dependent on peptide concentration. Co-cultures of the bacteria resulted in the highest ACE inhibitory and antioxidant activities.
FERMENTATION-BASEL
(2022)
Article
Microbiology
Marcello Brugnoli, Ilaria Mazzini, Salvatore La China, Luciana De Vero, Maria Gullo
Summary: In this study, a co-culture system combining bacterial cellulose (BC) producers and hyaluronic acid (HA) producers was developed to investigate the chemical and morphological structure of BC-HA composites. The presence of HA increased BC yield and altered the fiber dimension and crystallinity of the composites. Different results were observed based on the combination of BC producer and HA producer. The water holding capacity improved with the presence of HA, while water uptake worsened. A thymol-enriched BC-HA composite showed high antibacterial activity. Results could have applications in the cosmetics or pharmaceutical fields.
Article
Biotechnology & Applied Microbiology
Suresh Panthee, Atmika Paudel, Hiroshi Hamamoto, Akihiko Ano Ogasawara, Toshihiro Iwasa, Jochen Blom, Kazuhisa Sekimizu
Summary: Enterococcus faecalis is a common human gut commensal bacterium with potential pathogenic or probiotic properties. Genome sequencing and analysis of the probiotic strain EF-2001 revealed unique characteristics, aiding in the differentiation of different E. faecalis strains.
Article
Microbiology
Zongjie Li, Yuhao Li, Changguang Xiao, Zujie Yan, Ruyi Pan, Yun Gao, Beibei Li, Jianchao Wei, Yafeng Qiu, Ke Liu, Donghua Shao, Zhiyong Ma
Summary: This research sequenced and analyzed the genome of L. sakei JD10 strain isolated from porcine lung. The genome analysis revealed genes responsible for the production of inhibitory substances, carbohydrate degradation enzymes, and short chain fatty acids, as well as their regulatory effects on bacterial phagocytosis. Bacteriocin-producing genes and CRISPR-Cas system related genes were also predicted in the genome. Overall, L. sakei JD10 showed potential probiotic traits in fighting pathogenic infection and regulating host immune function.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Franco Segli, Constanza Melian, Virginia Munoz, Graciela Vignolo, Patricia Castellano
Summary: The bioprotective extracts from Lactobacillus acidophilus were effective in completely inhibiting spoilage strains in refrigerated meat, while extracts from Latilactobacillus curvatus had a bacteriostatic effect when combined with L. acidophilus. The use of more accurate tools to prevent the growth of deteriorating species can contribute to extending the shelf-life of fresh meat without compromising quality.
Article
Food Science & Technology
Yaozu Guo, Wenlong Ma, Manxi Song, Wenqiong Wang, Boxing Yin, Ruixia Gu
Summary: In this study, a combination of LAB fermentation and beta-galactosidase catalysis methods was used to increase lactulose concentration and reduce galactose and lactose content. The optimal conditions for chemical isomerization were found to be 70 degrees C for 50 min, resulting in a lactulose concentration of 31.3 +/- 1.2%. Two selected LAB strains, Lactobacillus salivarius TM-2-8 and Lactobacillus rhamnosus grx.21, effectively fermented the chemical isomerization system, leading to decreased lactose and galactose content. The enzymatic concentration of 3 U/mL and a catalysis time of 3 h at 50 degrees C using beta-galactosidase achieved the highest lactulose concentration of 74.89 +/- 1.68 g/L.
Article
Thermodynamics
Ciro Vasmara, Rosa Marchetti, Domenico Carminati
Summary: This study evaluated the biogas production potential of fermentation wastewaters from two strains of lactic acid bacteria, showing that they are suitable feedstocks for biogas production and can be directly mixed with pig slurry to enhance methane yield and shorten the digestion process. The digestate could also be utilized as crop fertilizer from a renewable source.
Review
Agriculture, Dairy & Animal Science
Shuo Yang, Xiaoqing Xu, Qing Peng, Lan Ma, Yu Qiao, Bo Shi
Summary: The over-use or misuse of antibiotics in livestock and poultry production has led to a rising threat of antibiotic resistance in animals and negative ecological effects. Exopolysaccharides from lactic acid bacteria (LAB-EPS) have been found to have anti-inflammatory and antimicrobial activities, and can regulate intestinal health and the immune system in livestock. LAB-EPS are considered as ideal alternatives to antibiotics due to their biodegradability, non-toxicity, and bio-compatibility.
Article
Biotechnology & Applied Microbiology
F. L. Paganelli, M. de Been, J. C. Braat, T. Hoogenboezem, C. Vink, J. Bayjanov, M. R. C. Rogers, J. Huebner, M. J. M. Bonten, R. J. L. Willems, H. L. Leavis
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2015)
Article
Microbiology
Maria Elena Martino, Jumamurat R. Bayjanov, Brian E. Caffrey, Michiel Wels, Pauline Joncour, Sandrine Hughes, Benjamin Gillet, Michiel Kleerebezem, Sacha A. F. T. van Hijum, Francois Leulier
ENVIRONMENTAL MICROBIOLOGY
(2016)
Article
Microbiology
Elena Buelow, Teresita D. J. Bello Gonzalez, Susana Fuentes, Wouter A. A. de Steenhuijsen Piters, Leo Lahti, Jumamurat R. Bayjanov, Eline A. M. Majoor, Johanna C. Braat, Maaike S. M. van Mourik, Evelien A. N. Oostdijk, Rob J. L. Willems, Marc J. M. Bonten, Mark W. J. van Passel, Hauke Smidt, Willem van Schaik
Article
Engineering, Biomedical
Miquel B. Ekkelenkamp, Mats T. Vervoorn, Jumamurat R. Bayjanov, Ad C. Fluit, Barry J. Benaissa-Trouw, Faiz Z. Ramjankhan
Article
Public, Environmental & Occupational Health
M. J. D. Dautzenberg, J. R. Bayjanov, M. A. Leverstein-van Hall, A. E. Muller, L. B. S. Gelinck, C. L. Jansen, E. M. S. Leyten, T. Ruys, J. Scharringa, R. E. van der Starre, A. C. Fluit, M. J. M. Bonten
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2018)
Meeting Abstract
Toxicology
J. R. Bayjanov, J. C. Kleinjans, D. G. J. Jennen
TOXICOLOGY LETTERS
(2018)
Article
Genetics & Heredity
Jumamurat R. Bayjanov, Jery Baan, Malbert R. C. Rogers, Annet Troelstra, Rob J. L. Willems, Willem van Schaik
MICROBIAL GENOMICS
(2019)
Article
Microbiology
Miquel B. Ekkelenkamp, Rafael Canton, Maria Diez-Aguilar, Michael M. Tunney, Deirdre F. Gilpin, Francesca Bernardini, Glenn E. Dale, J. Stuart Elborn, Jumamurat R. Bayjanov, Ad Fluit
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2020)
Article
Microbiology
Jumamurat R. Bayjanov, Miquel B. Ekkelenkamp, Malbert R. C. Rogers, Rafael Canton, Barry J. Benaissa-Trouw, Maria Diez-Aguilar, Michael Tunney, Ad C. Fluit
FUTURE MICROBIOLOGY
(2019)
Article
Infectious Diseases
Naouale Maataoui, Celine Langendorf, Fatou Berthe, Jumamurat R. Bayjanov, Willem van Schaik, Sheila Isanaka, Rebecca F. Grais, Olivier Clermont, Antoine Andremont, Laurence Armand-Lefevre, Paul-Louis Woerther
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2020)
Article
Microbiology
Ad C. Fluit, Jumamurat R. Bayjanov, Michael Tunney, J. Stuart Elborn, Malbert R. C. Rogers, Anita C. Schurch, Miguel B. Ekkelenkamp
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2019)
Article
Microbiology
Elena Buelow, Jumamurat R. Bayjanov, Eline Majoor, Rob J. L. Willems, Marc J. M. Bonten, Heike Schmitt, Willem van Schaik
FEMS MICROBIOLOGY ECOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Xinglin Zhang, Vincent de Maat, Ana M. Guzman Prieto, Tomasz K. Prajsnar, Jumamurat R. Bayjanov, Mark de Been, Malbert R. C. Rogers, Marc J. M. Bonten, Stephane Mesnage, Rob J. L. Willems, Willem van Schaik
Article
Microbiology
Ana M. Guzman Prieto, Jessica Wijngaarden, Johanna C. Braat, Malbert R. C. Rogers, Eline Majoor, Ellen C. Brouwer, Xinglin Zhang, Jumamurat R. Bayjanov, Marc J. M. Bonten, Rob J. L. Willems, Willem van Schaik
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2017)
Article
Microbiology
Ad C. Fluit, Miquel B. Ekkelenkamp, Michael M. Tunney, J. Stuart Elborn, Malbert R. C. Rogers, Jumamurat R. Bayjanov
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2020)