Article
Microbiology
Julia Ittensohn, Jacqueline Hemberger, Hannah Griffiths, Maren Keller, Simone Albrecht, Thomas Miethke
Summary: The study demonstrates that the uropathogenic Escherichia coli strain CFT073 causes kidney abscesses in mice through the TcpC gene, which is present in around 40% of E. coli isolates of pyelonephritis patients. TcpC impairs host immune response by affecting the TLR signaling chain and NLRP3 inflammasome.
Article
Biochemistry & Molecular Biology
Juan Xu, Hao Guo, Lirong Li, Fang He
Summary: This study identified 27 carbapenem-resistant Escherichia coli (CREC) strains, of which 17 carried the blaNDM-5 gene. All 23 NDM-producing E. coli strains were resistant to all antibiotics except tigecycline, colistin, and cefiderocol. Furthermore, two copies of the blaNDM-5 gene were found on a single plasmid, highlighting the need for attention.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Microbiology
Jin-Tao Yang, Li-Juan Zhang, Yang Lu, Rong-Min Zhang, Hong-Xia Jiang
Summary: CTX-M-55 was first discovered in Thailand in 2004 and has become the most common subtype of CTX-M enzyme in animal-origin E. coli in China. The widespread dissemination of bla(CTX-M-55)-positive E. coli poses a growing public health problem. This study constructed a global genomic data set of bla(CTX-M-55)-positive E. coli and explored its epidemiology and potential impact using high-resolution bioinformatics methods.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Guoli Li, Xinyang Li, Yuye Wu, Juan Xu, Fang He
Summary: This study reveals the emergence and dissemination of colistin-resistant E. coli isolates carrying mcr-1 in a Chinese hospital, posing a potential risk to anti-infective therapy. The study also shows the resistance and genetic characteristics of these strains.
Article
Biochemistry & Molecular Biology
Layane A. L. Silva, Andre A. L. Silva, Maria A. S. Rios, Manoel P. Brito, Alyne R. Araujo, Durcilene A. Silva, Ramon R. Pena-Garcia, Edson C. Silva-Filho, Janildo L. Magalhaes, Jose M. E. Matos, Josy A. Osajima, Eduardo R. Triboni
Summary: This study investigated the effects of copper doping of hydrated cobalt molybdate on its antibacterial activity and structural changes, demonstrating that increasing copper content led to larger microparticle size and better bactericidal performance against gram-positive bacteria than gram-negative bacteria. However, the use of copper as a doping agent caused an increase in the Minimum Inhibitory Concentration value in the presence of both Escherichia coli and Staphylococcus aureus strains, indicating the need for caution in utilizing copper in biocidal matrices.
Review
Biochemistry & Molecular Biology
Sonia Mondino, Fabiana San Martin, Alejandro Buschiazzo
Summary: Bacterial flagella are nanomachines that enable high-speed cell movement. Recent breakthroughs in 3D cryoEM methods have revealed novel structures and mechanistic insights into these flagella, challenging previous theories. These discoveries have advanced our understanding of the three major segments of the flagellum.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Infectious Diseases
Zhouhui Wu, Yu Wang, Lei Li, Sihui Zhen, Heng Du, Zhiwen Wang, Shuang Xiao, Jinliang Wu, Lifei Zhu, Jiachen Shen, Zhen Wang
Summary: The bioreductive agent tirapazamine (TPZ) has significant antibacterial activity against E. coli and can reverse drug resistance, improve survival rate, and alleviate pathological damages in infected mice. TPZ also alters the structure of the gut microbiota. Therefore, TPZ shows promise as an effective antimicrobial agent for the treatment of E. coli infections.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Food Science & Technology
Aricia Possas, Fernando Perez-Rodriguez
Summary: Cross-contamination in vegetables and fruits has been studied in this review, emphasizing the significant impact of water-mediated microbial transfer on pathogen prevalence levels in produce. The review highlights the prime role of cross-contamination in illness burden and large outbreaks, and suggests effective strategies to reduce cross-contamination.
CURRENT OPINION IN FOOD SCIENCE
(2023)
Article
Microbiology
Alexis Dereeper, Nina Allouch, Vincent Guerlais, Maelle Garnier, Laurence Ma, Johan F. F. De Jonckheere, Sandeep J. J. Joseph, Ibne Karim M. Ali, Antoine Talarmin, Isabel Marcelino
Summary: In this study, a high-quality Naegleria pangenome was constructed, revealing that Naegleria may use genome plasticity features to modulate their behavior in different environments. Comparing 14 near-to-complete genome sequences, it was estimated that the Naegleria pangenome consists of 13,943 genes, including 3,563 core and 10,380 accessory genes. Functional annotations showed that a large fraction of Naegleria genes have significant sequence similarity with those in other kingdoms. Comparative analyses showed remarkable genomic heterogeneity in Naegleria, even among closely related strains, indicating extensive genome variability.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Infectious Diseases
Juan Li, Yuqin Song, Jianping Deng, Zhaoran Wang, Nai-Kei Wong, Chao Wang, Gang Zhang, Yang Wang, Shan Lu, Jie Che, Xiaofei Zhao, Zhengdong Zhang, Hong Wang, Ling Zhang, Yunfei Zhang, Xuemei Bai, Min Yuan, Xia Chen, Wen Zhang, Yanwen Xiong, Biao Kan, Jie Feng
Summary: This study explores the dynamics of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) transmission between food-producing animals and humans. The findings suggest that transmission primarily occurs through the high exposure group, which plays a crucial role as a medium, and the carriage rates of shared ARGs are higher in this group compared to the low exposure group.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Multidisciplinary Sciences
Ruggero La Rosa, Elio Rossi, Adam M. Feist, Helle Krogh Johansen, Soren Molin
Summary: Long-term infection with Pseudomonas aeruginosa in cystic fibrosis patients often leads to a reduction in bacterial growth rate. However, adaptive laboratory evolution can revert slow growth phenotype back to a high growth rate, increasing antibiotic susceptibility. This is achieved through evolutionary trajectories and mechanisms involving transcriptional and mutational changes, suggesting potential involvement in pathogen adaptation and chronic infections in antibiotic-treated patients with cystic fibrosis.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Linshu Lan, Hui Shi
Summary: Escherichia coli O157:H7 can be injured by lactic acid, with related genes changes in carbohydrate metabolism and osmotic stress response during sublethal injury. Recovery of injured cells involves upregulation of genes related to membrane properties, cellular processes, and virulence factors, providing insights into how the bacteria respond to stress and regain virulence.
Article
Biochemistry & Molecular Biology
Min Jiao, Wenbo He, Zhenlin Ouyang, Qian Qin, Yucheng Guo, Jiaxin Zhang, Yixin Bai, Xiaolong Guo, Qinyue Yu, Junjun She, Peter M. Hwang, Fang Zheng, Yurong Wen
Summary: PaaY is a bifunctional enzyme in Acinetobacter baumannii that acts as a thioesterase and a γ-carbonic anhydrase. The crystal structure of AbPaaY reveals a homotrimer with a unique domain-swapped C-termini, which affects its stability and substrate specificity. Knockout of AbPaaY impairs the growth, biofilm formation, and hydrogen peroxide resistance of Acinetobacter.
Article
Immunology
Bryan Augusto da Rosa Tavares, Jessica Andrade Paes, Arnaldo Zaha, Henrique Bunselmeyer Ferreira
Summary: This study reannotated the function of unknown proteins in M. hyopneumoniae using available databases and bioinformatic tools, predicting new potential virulence factors and improving the functional annotation of the genome.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Penghang Zhang, Xiaoai Zhang, Yuzhu Liu, Qingpo Cui, Xiaoxia Qin, Yanlin Niu, Chao Wang, TongYu Wang, Qian Chen, Shuangyang Ding, Xiaochen Ma, Zhangqi Shen
Summary: The proportion of campylobacteriosis caused by C. coli is rapidly increasing in China, with poultry products being the main source of infection in humans. The study reveals that multidrug-resistant C. coli strains have become prevalent since the 2010s, coinciding with the shift in abundance from C. jejuni to C. coli in Chinese poultry. These drug-resistant C. coli strains are well-adapted and proliferate in the poultry production environment, where multiple antimicrobial agents are frequently used. This study provides empirical evidence for the molecular mechanisms behind the dominance of C. coli in poultry and highlights the importance of poultry products as sources of C. coli infections in humans.
Article
Biochemical Research Methods
Wai Lim Ku, Kosuke Nakamura, Weiwu Gao, Kairong Cui, Gangqing Hu, Qingsong Tang, Bing Ni, Keji Zhao
Article
Immunology
Chao Zhong, Mingzhu Zheng, Kairong Cui, Andrew J. Martins, Gangqing Hu, Dan Li, Lino Tessarollo, Serguei Kozlov, Jonathan R. Keller, John S. Tsang, Keji Zhao, Jinfang Zhu
Article
Cell & Tissue Engineering
Zhilin Ma, Jian Xu, Limei Wu, Junjie Wang, Qiqi Lin, Fabliha Ahmed Chowdhury, Habibul Hasan Mazumder, Gangqing Hu, Xue Li, Wei Du
Article
Oncology
Jae Young So, Nicolas Skrypek, Howard H. Yang, Anand S. Merchant, George W. Nelson, Wei-Dong Chen, Hiroki Ishii, Jennifer M. Chen, Gangqing Hu, Bhagelu R. Achyut, Esther C. Yoon, Liying Han, Chuanshu Huang, Margaret C. Cam, Keji Zhao, Maxwell P. Lee, Li Yang
Article
Cell Biology
Tingliang Wang, Ryan C. Hill, Monika Dzieciatkowska, Lian Zhu, Aniello M. Infante, Gangqing Hu, Kirk C. Hansen, Ming Pei
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Oncology
Tayvia Brownmiller, Jamie A. Juric, Abby D. Ivey, Brandon M. Harvey, Emily S. Westemeier, Michael T. Winters, Alyson M. Stevens, Alana N. Stanley, Karen E. Hayes, Samuel A. Sprowls, Amanda S. Gatesman Ammer, Mackenzee Walker, Erik A. Bey, Xiaoliang Wu, Zuan-Fu Lim, Lin Zhu, Sijin Wen, Gangqing Hu, Patrick C. Ma, Ivan Martinez
Review
Pharmacology & Pharmacy
Wei-Chih Chen, Gangqing Hu, Lori A. Hazlehurst
CURRENT OPINION IN PHARMACOLOGY
(2020)
Article
Engineering, Biomedical
Yiming Wang, Gangqing Hu, Ryan C. Hill, Monika Dzieciatkowska, Kirk C. Hansen, Xiao-Bing Zhang, Zuoqin Yan, Ming Pei
Summary: This study demonstrates that expansion on decellularized extracellular matrix (dECM) can rejuvenate high-passage infrapatellar fat pad stem cells (IPFSCs) by reversing cellular senescence. SV40LT transduced IPFSCs showed increased proliferation and adipogenic potential but decreased chondrogenic potential. Matrix components like basement membrane proteins were identified as key factors in matrix-mediated rejuvenation of IPFSCs.
Article
Multidisciplinary Sciences
Megan E. Grund, Soo J. Choi, Dudley H. McNitt, Mariette Barbier, Gangqing Hu, P. Rocco LaSala, Christopher K. Cote, Rita Berisio, Slawomir Lukomski
Article
Biochemistry & Molecular Biology
Weiwu Gao, Wai Lim Ku, Lixia Pan, Jonathan Perrie, Tingting Zhao, Gangqing Hu, Yuzhang Wu, Jun Zhu, Bing Ni, Keji Zhao
Summary: The novel indexing strategy of iscDNase-seq enables profiling of chromatin accessibility in individual cells, revealing epigenomic variability and regulatory elements among them. This technique accurately identifies cell types and predicts gene expression variability, showing higher correlation compared to scATAC-seq and scRNA-seq. iscDNase-seq provides a promising alternative method for single-cell epigenomics studies.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Mingzhu Zheng, Kairui Mao, Difeng Fang, Dan Li, Jun Lyu, Dingkang Peng, Xi Chen, Nikki Cannon, Gangqing Hu, Jiajia Han, Keji Zhao, Wanjun Chen, Jinfang Zhu
Summary: IgA production in the gut relies on IgA-producing plasma cells derived from conventional B cells, with T cells and helper-like ILCs playing crucial roles in the formation of lymphoid structures in the gut. However, the functions of non-LTi helper-like ILCs in promoting IgA production are still not well understood.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Osama M. Elzamzamy, Brandon E. Johnson, Wei-Chih Chen, Gangqing Hu, Reinhold Penner, Lori A. Hazlehurst
Summary: The study investigated the treatment mechanism of MTI-101 for multiple myeloma (MM) and found that MTI-101 can induce Ca2+ and Na+ flux through TRPC1 heteromers. The formation of TRPC1-calcium-regulating protein STIM1 complex plays a key role in MTI-101-induced cell death.
Article
Oncology
Sebastian A. Dziadowicz, Lei Wang, Halima Akhter, Drake Aesoph, Tulika Sharma, Donald A. Adjeroh, Lori A. Hazlehurst, Gangqing Hu
Summary: The bone marrow microenvironment can protect multiple myeloma (MM) cells from therapeutic agents, leading to drug resistance. This study aimed to understand the mechanisms of de novo multi-drug resistance induced by interactions between MM cells and bone marrow stromal cells (BMSCs). Through genome-wide analysis, we identified changes in gene expression and chromatin accessibility in MM cells interacting with BMSCs. We found that BMSC-derived soluble factors and physical adhesion can induce distinct changes in the transcriptome and regulome of MM cells. Furthermore, we identified candidate transcription factors that regulate the BMSC-induced transcriptome and modulate the regulome. These findings provide valuable insights into the epigenetic mechanisms of BMSC-induced multi-drug resistance in MM.
Article
Oncology
Clark Jones, Sebastian Dziadowicz, Samuel Suite, Ashley Eby, Wei-Chih Chen, Gangqing Hu, Lori A. Hazlehurst
Summary: MTI-101, a novel cyclic peptide, has shown anti-tumor activity in multiple myeloma and castrate-resistant prostate cancer models. It induces a change in cell state in lung cancer cell lines, leading to increased sensitivity to EGFR inhibitors and synergistic effects with standard of care agents. Additionally, it reduces bone metastasis occurrence. These findings highlight the potential therapeutic value of chronic MTI-101 treatment.
Article
Oncology
Lei Wang, Weijun Yi, Li Ma, Emily Lecea, Lori A. Hazlehurst, Donald A. Adjeroh, Gangqing Hu
Summary: This study found that bone marrow mesenchymal stem cells (MSCs) in multiple myeloma patients have tumor-supportive and inflammatory characteristics, contributing to drug resistance. In vitro expansion of MSCs leads to the loss of inflammatory signature, but cytokine stimulation and coculture with immune cells or cancer cells can reactivate these characteristics. These findings provide an important foundation for further research on the role of inflammation in the tumor-supportive functions of bone marrow MSCs in disease progression and therapy resistance.