Article
Infectious Diseases
Jee Hong Kim, Yun Young Cho, Ji Young Choi, Yu Mi Wi, Kwan Soo Ko
Summary: This study investigated the characteristics of KPC-2-producing Klebsiella pneumoniae isolates from a hospital in South Korea. It identified two main clones, ST11 and ST307, with different resistance profiles and virulence levels. Continuous monitoring of these clones, which show different characteristics including resistance and virulence, is necessary.
Article
Infectious Diseases
Carolina Venditti, Ornella Butera, Marcello Meledandri, Maria Pia Balice, Giulio Cesare Cocciolillo, Carla Fontana, Silvia D'Arezzo, Chiara De Giuli, Mario Antonini, Alessandro Capone, Francesco Messina, Carla Nisii, Antonino Di Caro
Summary: The study analyzed ceftazidime-avibactam-resistant KPC-producing Klebsiella pneumoniae strains collected from 31 patients in six hospitals in Rome, revealing mutations in KPC variants within the bla(KPC-3) gene and the presence of high-risk clones (ST512, 101, and 307). The findings highlight concerns over the potential selection of a multidrug-resistant phenotype in strains showing resistance to both ceftazidime-avibactam and carbapenems.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Multidisciplinary Sciences
Joshua L. C. Wong, Sophia David, Julia Sanchez-Garrido, Jia Z. Woo, Wen Wen Low, Fabio Morecchiato, Tommaso Giani, Gian Maria Rossolini, Konstantinos Beis, Stephen J. Brett, Abigail Clements, David M. Aanensen, Silvi Rouskin, Gad Frankel
Summary: This study investigates the impact of mutations in OmpK36 on carbapenem resistance in Klebsiella pneumoniae. The researchers found that a recurrent 25c > t transition in ompK36 increases resistance by reducing the expression of OmpK36 in the outer membrane. This mutation attenuates the bacteria in a mouse pneumonia model but leads to treatment failure in the context of carbapenem therapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Infectious Diseases
Rania Abd El-Hamid El-Kady, Mohamed Ali Elbaiomy, Rasha Mokhtar Elnagar
Summary: This study investigated the susceptibility profile of CRKP isolates from cancer patients to CZA and identified the underlying resistance mechanisms. The study found risk factors and mechanisms associated with CZA resistance.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Infectious Diseases
Travis J. Kochan, Sophia H. Nozick, Rachel L. Medernach, Bettina H. Cheung, Samuel W. M. Gatesy, Marine Lebrun-Corbin, Sumitra D. Mitra, Natalia Khalatyan, Fiorella Krapp, Chao Qi, Egon A. Ozer, Alan R. Hauser
Summary: This study found that both multidrug-resistant high-risk clones and hypervirulent K. pneumoniae isolates are present in bloodstream isolates in the United States. It is particularly concerning that hypervirulent isolates are undergoing community spread in the United States, and these isolates are unrelated to travel history to Asia.
BMC INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Mitra Ahmadi, Reza Ranjbar, Payam Behzadi, Taher Mohammadian
Summary: This study identified and characterized clinical strains of Klebsiella pneumoniae, revealing a high prevalence of antibiotic resistance and virulence genes. The study highlights the significant pathogenic potential of isolated pathotypes of K. pneumoniae.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2022)
Article
Microbiology
Deyan Donchev, Ivan N. N. Ivanov, Ivan Stoikov, Stefana Sabtcheva, Yordan Kalchev, Marianna Murdjeva, Elina Dobreva, Rumyana Hristova
Summary: The study improved and extended the multi-locus VNTR analysis (MLVA) for strain-level differentiation of Klebsiella pneumoniae species complex. The modified scheme (MLVA8+) showed high concordance with classic MLST and provided greater differentiation capability. MLVA8+ also demonstrated its applicability in investigating the intra-hospital spread of carbapenem-resistant producers. It offers improved typing accuracy and extended typing scope for Klebsiella pneumoniae.
Article
Microbiology
Guoping Cai, Yan Wang
Summary: This study investigated the molecular patterns of clinically important fluoroquinolone resistance (CI-FR) in multidrug-resistant Klebsiella pneumoniae (MDR-KP) isolates in a tertiary hospital in Shanghai, PR China. The results showed that mutations in quinolone resistance-determining region (QRDR) and the acquisition of plasmid-mediated quinolone resistance (PMQR) genes played a role in the development of CI-FR. High-risk CRKP clones responsible for nosocomial outbreaks were also identified.
JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
Article
Microbiology
Meng Wang, Yujiao Tian, Li Xu, Fusheng Zhang, Huigai Lu, Moran Li, Bei Li
Summary: This study reveals that high osmotic stress in the environment can influence the resistance of Klebsiella pneumoniae to antibiotics. The newly found regulator KbvR can be upregulated under high osmotic stress and directly regulate the expression of OmpK36 to change the resistance of K. pneumoniae to beta-lactam antibiotics.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Qian Xu, Beiwen Zheng, Ping Shen, Yonghong Xiao
Summary: This study aimed to identify risk factors for mortality and severity in patients with bloodstream infection of Klebsiella pneumoniae (BSI-KP). The use of statins and quinolones was found to be protective against 28-day mortality, while the use of vasoactive drugs was a risk factor. Additionally, pulmonary disease, bleeding and coagulation disorders, respiratory failure, and kidney dysfunction were independent risk factors for septic shock in BSI-KP patients. A risk factor-based prediction model showed good performance in predicting mortality and septic shock in BSI-KP patients. Paying more attention to drug treatment strategies can improve patient outcomes in clinical practice.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Salud Rodriguez-Pallares, M. Alejandra Mateo-Vargas, Manuel A. Rodriguez-Iglesias, Fatima Galan-Sanchez
Summary: We studied the molecular characteristics of twenty consecutive OXA-48 producing K. pneumoniae isolates from six patients and found that they had different virulence profiles, with the possible acquisition of new virulence factor genes over time.
MICROBES AND INFECTION
(2023)
Article
Immunology
Luana Soares de Moraes, Gerusa Luciana Gomes Magalhaes, Joao Gabriel Material Soncini, Marsileni Pelisson, Marcia Regina Eches Perugini, Eliana Carolina Vespero
Summary: This study evaluated clinical data from 107 patients with bloodstream infection caused by Klebsiella pneumoniae and conducted phenotypic and molecular analyses on 50.5% (54/107) of samples that exhibited resistance to carbapenems. The bla(NDM) gene was present in 90.4% (49/54) of these samples, bla(KPC) gene was found in one sample, and 7.4% (4/54) of samples showed no carbapenemase gene. The study identified four main clones and found that 11 samples were not genetically related. The median age of the patients was 58 years old, with a majority of males (60.7%). Comparing patients with and without carbapenem resistance, several factors including ICU stay, renal failure, previous antimicrobial use, Charlson's comorbidity index, invasive procedures, and death showed statistically significant differences. Additionally, renal failure, liver failure, and BSI with extensive-drug resistance (XDR) or pan-drug resistance (PDR) were predictors of increased mortality. The study concluded that patients with carbapenem-resistant pneumonia, regardless of additional resistance to polymyxins, experienced higher mortality rates.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Alessandra Carattoli, Gabriele Arcari, Giulia Bibbolino, Federica Sacco, Dario Tomolillo, Federica Maria Di Lella, Maria Trancassini, Luigi Faino, Mario Venditti, Guido Antonelli, Giammarco Raponi
Summary: A study conducted in a university hospital in Rome, Italy identified multiple CZA-resistant KPC-3 protein variants, including novel variants that had not been reported before. These variants showed different sensitivities and activities to antibiotics, possibly due to mutations caused by amino acid substitutions, insertions, and deletions.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Pilar Lumbreras-Iglesias, Maria Rosario Rodicio, Pablo Valledor, Tomas Suarez-Zarracina, Javier Fernandez
Summary: This study aimed to analyze the outer membrane porin-encoding genes in OXA-48 producing Klebsiella pneumoniae and assess their impact on ceftazidime/avibactam susceptibility, as well as to describe a screening methodology. Various OmpK36 alterations were detected in OXA-48-producing K. pneumoniae isolates, with high-level meropenem resistance. All isolates were susceptible to ceftazidime/avibactam, suggesting its potential as a choice therapy for these challenging isolates.
Article
Infectious Diseases
Jose Joao Sumbana, Antonella Santona, Nader Abdelmalek, Maura Fiamma, Massimo Deligios, Alice Manjate, Jahit Sacarlal, Salvatore Rubino, Bianca Paglietti
Summary: Globally, antibiotic-resistant Klebsiella spp. cause healthcare-associated infections with high mortality rates. The study investigated the phenotypic and genetic features of Klebsiella isolates from invasive infections in Mozambique. The majority of isolates displayed multidrug resistance, with ESBL-producing strains carrying various beta-lactamase genes. The study also identified susceptible hypervirulent Klebsiella pneumoniae isolates associated with severe infections. The findings highlight the importance of surveillance strategies to prevent the emergence of multidrug-resistant hypervirulent Klebsiella pneumoniae.
Article
Microbiology
Chuan Chiang-Ni, Po-Xing Zheng, Shu-Ying Wang, Pei-Jane Tsai, Woei-Jer Chuang, Yee-Shin Lin, Ching-Chuan Liu, Jiunn-Jong Wu
JOURNAL OF CLINICAL MICROBIOLOGY
(2016)
Article
Immunology
Po-Xing Zheng, Hsin-Yi Fang, Hsiao-Bai Yang, Nai-Yueh Tien, Ming-Cheng Wang, Jiunn-Jong Wu
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2016)
Article
Immunology
Po-Xing Zheng, Yuen-Chi Chan, Chien-Shun Chiou, Cheng-Lu Hsieh, Chuan Chiang-Ni, Jiunn-Jong Wu
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2017)
Article
Microbiology
Yi-Lin Cheng, Yan-Wei Wu, Chih-Feng Kuo, Shiou-Ling Lu, Fu-Tong Liu, Robert Anderson, Chiou-Feng Lin, Yi-Ling Liu, Wan-Yu Wang, Ying-Da Chen, Po-Xing Zheng, Jiunn-Jong Wu, Yee-Shin Lin
Letter
Infectious Diseases
Hung-Mo Chen, Po-Xing Zheng, Li-Rong Wang, Jiunn-Jong Wu, Jing-Jou Yan
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2017)
Article
Microbiology
Hsin-Yu Li, Cheng-Yen Kao, Wei-Hung Lin, Po-Xing Zheng, Jing-Jou Yan, Ming-Cheng Wang, Ching-Hao Teng, Chin-Chung Tseng, Jiunn-Jong Wu
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Microbiology
Cheng-Lu Hsieh, Hsuan-Min Huang, Shu-Ying Hsieh, Po-Xing Zheng, Yee-Shin Lin, Chuan Chiang-Ni, Pei-Jane Tsai, Shu-Ying Wang, Ching-Chuan Liu, Jiunn-Jong Wu
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Katlyn Borgers, Jheng-Yang Ou, Po-Xing Zheng, Petra Tiels, Annelies Van Hecke, Evelyn Plets, Gitte Michielsen, Nele Festjens, Nico Callewaert, Yao-Cheng Lin
Article
Microbiology
Cheng-Lu Hsieh, Shu-Ying Hsieh, Hsuan-Min Huang, Shiou-Ling Lu, Hiroko Omori, Po-Xing Zheng, Yen-Ning Ho, Yi-Lin Cheng, Yee-Shin Lin, Chuan Chiang-Ni, Pei-Jane Tsai, Shu-Ying Wang, Ching-Chuan Liu, Takeshi Noda, Jiunn-Jong Wu
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Microbiology
Chien-Jui Huang, Ting-Li Wu, Po-Xing Zheng, Jheng-Yang Ou, Hui-Fang Ni, Yao-Cheng Lin
Summary: Genomes of X. citri subsp. citri strains from citrus orchards in Taiwan with different management practices were sequenced, revealing genome diversity and distribution of metal resistance genes. The copper-resistant strain has a unique copper resistance plasmid, while copper sensitive strains lack metal resistance genes in the plasmid. Plasmids played an important role in maintaining pathogenicity and bacterial fitness, with potential trigger for the evolution of citrus canker pathogens.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Choun-Sea Lin, Chen-Tran Hsu, Yu-Hsuan Yuan, Po-Xing Zheng, Fu-Hui Wu, Qiao-Wei Cheng, Yu-Lin Wu, Ting-Li Wu, Steven Lin, Jin-Jun Yue, Ying-Huey Cheng, Shu- Lin, Ming-Che Shih, Jen Sheen, Yao-Cheng Lin
Summary: A DNA-free CRISPR-Cas9 genome editing tool based on an optimized protoplast regeneration protocol of wild tomato creates stable and inheritable diploid and tetraploid regenerants, which has significant implications for plant breeding and genetic research.
Article
Microbiology
Chien-Jui Huang, Po-Xing Zheng, Jheng-Yang Ou, Yao-Cheng Lin, Chao-Ying Chen
GENOME ANNOUNCEMENTS
(2017)
Article
Microbiology
Cheng-Yen Kao, Jing-Jou Yan, Yu-Chun Lin, Po-Xing Zheng, Jiunn-Jong Wu
GENOME ANNOUNCEMENTS
(2017)
Article
Microbiology
Wei-Hung Lin, Po-Xing Zheng, Tsunglin Liu, Chin-Chung Tseng, Wei-Chu Chen, Ming-Cheng Wang, Jiunn-Jong Wu
GENOME ANNOUNCEMENTS
(2016)