Article
Medicine, Research & Experimental
Xingxing Dong, Yanjie Chao, Yang Zhou, Rui Zhou, Wei Zhang, Vincent A. Fischetti, Xiaohong Wang, Ye Feng, Jinquan Li
Summary: Researchers have identified a new human-associated clade of Streptococcus suis, named HAC, originating from European swine breeds exported in the 1960s and 1970s. The formation of HAC reveals healthy-pig carriers as a potential source for human infection, and new HAC-specific genes serve as promising markers for pathogen detection and surveillance.
EMBO MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Maria Jose Lopez-Martinez, Andelo Beletic, Josipa Kules, Dina Resetar-Maslov, Ivana Rubic, Vladimir Mrljak, Edgar Garcia Manzanilla, Elena Goyena, Silvia Martinez-Subiela, Jose Joaquin Ceron, Alberto Munoz-Prieto
Summary: Through saliva and serum proteomic analysis, we elucidated specific proteome changes in saliva and serum caused by Streptococcus suis infection-induced meningitis, which may reflect different pathophysiological mechanisms and provide new potential biomarkers for this infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Esther Prados de la Torre, Antonio Rodriguez-Franco, Manuel J. Rodriguez-Ortega
Summary: This study compared the exoproteome fraction of different human clinical isolates of Streptococcus suis, revealing key proteins with antigenic potential and providing important insights for further research into the pathogenic mechanisms of this bacterium.
Article
Microbiology
Manon Dechene-Tempier, Eric Jouy, Marie-Helene Bayon-Auboyer, Stephanie Bougeard, Claire Chauvin, Virginie Libante, Sophie Payot, Corinne Marois-Crehan
Summary: A study in France analyzed the antimicrobial resistance of 200 strains of Streptococcus suis, a zoonotic pathogen causing infections in pigs. Most strains showed resistance to at least one antibiotic, with higher resistance to macrolides-lincosamides and tetracycline. Resistance patterns varied based on geographical origin, serotypes, and isolate sources. The study highlights the importance of monitoring antimicrobial resistance in this zoonotic pathogen.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Immunology
Ting Gao, Fei Ye, Yiqing Tan, Mingzheng Peng, Fangyan Yuan, Zewen Liu, Danna Zhou, Keli Yang, Wei Liu, Rui Guo, Tengfei Zhang, Lin Zheng, Rui Zhou, Yongxiang Tian
Summary: Epigallocatechin gallate (EGCG), a green tea polyphenol, has antibacterial effects against Streptococcus suis (S. suis) by inhibiting its growth, reducing its pathogenicity, and decreasing hemolytic activity and biofilm formation. EGCG may serve as a potential compound for the treatment of S. suis infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Biology
Hajarooba Gnanagobal, Javier Santander
Summary: This review provides a comprehensive analysis of the interactions between marine Gram-positive pathogens and fish hosts, which cause economic losses in global marine fish aquaculture. Understanding these host-pathogen interactions is crucial for developing effective prophylactic measures and treatments.
Article
Multidisciplinary Sciences
Warangkhana Songsungthong, Sunisa Prasopporn, Louise Bohan, Potjanee Srimanote, Ubolsree Leartsakulpanich, Suganya Yongkiettrakul
Summary: This study screened the Pathogen Box compound library and identified 30 compounds that effectively inhibit the growth of Streptococcus suis, with MMV675968 being the most potent. MMV675968 was found to target S. suis dihydrofolate reductase and showed better efficacy compared to other DHFR inhibitors, serving as a potential starting point for developing antibiotics against drug resistant S. suis.
Article
Genetics & Heredity
Simen Fredriksen, Suzanne D. E. Ruijten, Gemma G. R. Murray, Maria Juanpere-Borras, Peter van Baarlen, Jos Boekhorst, Jerry M. Wells
Summary: Streptococcus suis can colonize the upper respiratory tract of healthy pigs but can also cause disease. This study compared the gene expression of pathogenic and commensal strains of S. suis and found that although there is genomic divergence, the transcriptomes remain conserved when grown in active porcine serum. However, key pathways showed variation in regulation and expression.
MICROBIAL GENOMICS
(2023)
Article
Microbiology
Yun-Dan Zheng, Tairan Zhong, Haiming Wu, Nan Li, Zuye Fang, Linlin Cao, Xing-Feng Yin, Qing-Yu He, Ruiguang Ge, Xuesong Sun
Summary: Infections caused by drug-resistant bacteria are a serious problem worldwide. The repurposed drug crizotinib exhibits excellent antibacterial activity against drug-resistant bacteria by suppressing ATP production and pyrimidine metabolism. The unique mechanism of action of crizotinib, targeting the CTP synthase PyrG and reducing DNA synthesis, may explain the decreased development of resistance by Staphylococcus aureus. This study provides a potential option for the treatment of drug-resistant bacterial infections in the future.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Greg Tram, Freda E. -C. Jen, Zachary N. Phillips, Jamie Timms, Asma-Ul Husna, Michael P. Jennings, Patrick J. Blackall, John M. Atack
Summary: Streptococcus suis is a significant pathogen causing bacterial meningitis in humans and pigs. A Type III DNA methyltransferase called ModS in S. suis controls phasevarions through biphasic ON-OFF switching, influencing growth patterns and antibiotic resistance. Understanding ModS phase variation is crucial for developing treatments and vaccines against this important pathogen.
Article
Immunology
Alexa N. Lauer, Rene Scholtysik, Andreas Beineke, Christoph Georg Baums, Kristin Klose, Peter Valentin-Weigand, Hiroshi Ishikawa, Horst Schroten, Ludger Klein-Hitpass, Christian Schwerk
Summary: This study utilized RNA-seq to compare global transcriptome profiles of S. suis-infected in vitro cells and in vivo infected pigs, identifying genes involved in inflammatory responses and hypoxia. The results suggest that similar cellular processes occur in infected human and porcine CP epithelial cells, particularly in terms of inflammatory response.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Qiang Sun, Na Li, Li Jia, Wenfei Guo, Hexiang Jiang, Baijun Liu, Chuntong Bao, Mengmeng Liu, Jing Huang, Liancheng Lei
Summary: RPSA(+) PMNs possess a dual role in combating Streptococcus suis serotype 2 infection, enhancing bacterial clearance while reducing inflammatory responses. However, their disruption of the BBB integrity presents a potential drawback to their therapeutic potential.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Veterinary Sciences
Yanyan Zhang, Rui Li, Qian Li, Yongwei Zhu, Xiaopei Yang, Di Zhao, Bingbing Zong
Summary: This study investigates the regulative functions of CovR in the survivability and pathogenicity of Streptococcus suis serotype 2 (S. suis 2) isolated from a diseased pig. The deletion of CovR significantly weakens the growth rates, capsular polysaccharides, adhesion, invasion, and anti-killing abilities of S. suis 2. It also reduces colonization ability in mice and leads to changes in metabolism-related pathways.
BMC VETERINARY RESEARCH
(2023)
Article
Infectious Diseases
Luis Fernandez-Espigares, Itahisa Hernandez-Chico, Manuela Exposito-Ruiz, Antonio Rosales-Castillo, Jose Maria Navarro-Mari, Jose Gutierrez-Fernandez
Summary: This study analyzed the epidemiology and antibiotic susceptibility of significant bacteriuria and found that the adoption of EUCAST criteria did not lead to a general change in resistance rates. The results showed stable resistance profiles for most antibiotics, with exceptions for levofloxacin and nitrofurantoin. The prevalence of methicillin-resistant Staphylococcus aureus was similar in hospital and community samples. The study suggests the need to revise certain empirical criteria for Enterococcus and community-origin S. aureus.
Article
Genetics & Heredity
Hamed Khakzad, Lotta Happonen, Guy Tran Van Nhieu, Johan Malmstrom, Lars Malmstrom
Summary: In this study, protein-protein interactions within the human complement system membrane attack complex (MAC) were investigated using in vivo cross-linking mass spectrometry and computational modeling. Over 100 cross-linked lysine-lysine pairs were identified between different MAC components, leading to the proposal of a quaternary model of the assembled MAC in its native environment. The approach demonstrated the validity of the MAC model through comparison with existing X-ray crystallographic and electron cryo-microscopic models, showcasing the potential of this method in studying native protein interactions.
FRONTIERS IN GENETICS
(2021)
Article
Immunology
Esther Prados de la Torre, Antonio Rodriguez-Franco, Manuel J. Rodriguez-Ortega
Article
Immunology
Angela Galan-Relano, Lidia Gomez-Gascon, Antonio Rodriguez-Franco, Inmaculada Luque, Belen Huerta, Carmen Tarradas, Manuel J. Rodriguez-Ortega
Article
Biochemistry & Molecular Biology
Jose A. Martina, David Guerrero-Gomez, Eva Gomez-Orte, Jose Antonio Barcena, Juan Cabello, Antonio Miranda-Vizuete, Rosa Puertollano
Summary: Mammalian TFEB and TFE3, as well as their ortholog in Caenorhabditis elegans HLH-30, are regulated by a novel mechanism involving cysteine-mediated redox switch under stress conditions, leading to oligomer formation and increased stability to stress. Cysteine oxidation acts as a molecular switch linking redox balance changes with expression of target genes.
Article
Veterinary Sciences
Jose Maria Sanchez-Carvajal, Angela Galan-Relano, Ines Ruedas-Torres, Francisco Jurado-Martos, Fernanda Larenas-Munoz, Eduardo Vera, Lidia Gomez-Gascon, Fernando Cardoso-Toset, Irene Magdalena Rodriguez-Gomez, Alfonso Maldonado, Librado Carrasco, Carmen Tarradas, Jaime Gomez-Laguna, Inmaculada Luque
Summary: The study demonstrated that qPCR targeting IS6110 is a rapid and accurate complementary diagnostic method for bovine tuberculosis, reducing the number of samples needing microbiological culture, and decreasing associated costs and turnaround time for diagnosis.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Microbiology
Esther Prados de la Torre, Antonio Rodriguez-Franco, Manuel J. Rodriguez-Ortega
Summary: This study compared the exoproteome fraction of different human clinical isolates of Streptococcus suis, revealing key proteins with antigenic potential and providing important insights for further research into the pathogenic mechanisms of this bacterium.
Article
Microbiology
Alfonso Olaya-Abril, Jose A. Gonzalez-Reyes, Manuel J. Rodriguez-Ortega
Summary: Infections caused by Streptococcus pneumoniae pose a major global health threat, with significant differences in surface structures and proteins among different strains under varying conditions. Understanding the interactions between this pathogen and the host can provide insights for strategies to combat these infections.
Article
Biotechnology & Applied Microbiology
F. C. De Aguiar, A. L. Solarte, L. Gomez-Gascon, A. Galan-Relano, I Luque, C. Tarradas, M. J. Rodriguez-Ortega, B. Huerta
Summary: The study found that cinnamon, common thyme, and red thyme essential oils have potential bactericidal activity against Streptococcus suis, making them a promising antimicrobial option for controlling this emerging zoonotic pathogen in veterinary medicine.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Immunology
Yanli Li, Enric Mateu, Ivan Diaz
Summary: This study examined the impact of freezing porcine PBMC on cell viability and functionality. After one month in liquid nitrogen, cell viability was above 89%, and PBMC response to mitogens showed similarities to fresh cells. However, frozen PBMC exhibited impaired antigen-specific responses, particularly in proliferation assays, suggesting a potential impact on effector/memory cells but not on naïve T cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Alfonso Olaya-Abril, Rafael Prados-Rosales, Jose A. Gonzalez-Reyes, Arturo Casadevall, Liise-anne Pirofski, Manuel J. Rodriguez-Ortega
Summary: This study demonstrates that extracellular vesicles produced by different serotypes of Streptococcus pneumoniae can be internalized by macrophages through fusion with host cell membranes, and have the ability to induce an immune response and promote the production of proinflammatory cytokines, with higher levels observed in certain serotypes.
Article
Veterinary Sciences
Fernanda Larenas-Munoz, Jose M. Sanchez-Carvajal, Angela Galan-Relano, Ines Ruedas-Torres, Eduardo Vera-Salmoral, Lidia Gomez-Gascon, Alfonso Maldonado, Librado Carrasco, Carmen Tarradas, Inmaculada Luque, Irene M. Rodriguez-Gomez, Jaime Gomez-Laguna
Summary: This study emphasizes the role of histopathology in the systematic diagnosis of bovine tuberculosis (bTB), showing that it can rapidly confirm the results of real-time PCR and bacteriology. Histopathology plays a significant role in the control and monitoring of bTB.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Infectious Diseases
Angela Galan-Relano, Alfonso Maldonado, Lidia Gomez-Gascon, Carmen Tarradas, Rafael Jesus Astorga, Inmaculada Luque, Belen Huerta
Summary: Canine leishmaniasis is a parasitic disease that affects dogs and wild canids. This study aimed to evaluate the diagnostic value of clinical signs associated with this disease and explore the best symptom combinations for predicting the diagnosis. The results showed that alopecia and epistaxis were the most useful signs for supporting the diagnosis, while lameness also had diagnostic value. Combinations of alopecia with hyperkeratosis of the snout and alopecia with onychogryphosis showed the highest predictive validity.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Virology
Esther Prados de la Torre, Ignacio Obando, Marta Vidal, Beatriz de Felipe, Ruth Aguilar, Luis Izquierdo, Carlo Carolis, Peter Olbrich, Ana Capilla-Miranda, Pau Serra, Pere Santamaria, Pilar Blanco-Lobo, Gemma Moncunill, Manuel J. Rodriguez-Ortega, Carlota Dobano
Summary: SARS-CoV-2 infection has become a global health problem, especially with the emergence of new variants. Healthcare workers have been severely affected, and children have also been affected, some of whom have developed Pediatric Inflammatory Multisystem Syndrome Temporally Associated with SARS-CoV-2. The study highlights the differences in antibody levels between different demographic and clinical groups, as well as the immune response to different variants of concern.
Article
Veterinary Sciences
Francisco Jurado-Martos, Fernando Cardoso-Toset, Carmen Tarradas, Angela Galan-Relano, Jose Maria Sanchez-Carvajal, Ines Ruedas-Torre, Eduardo Vera, Fernanda Larenas-Munoz, Lidia Gomez-Gascon, Irene Magdalena Rodriguez-Gomez, Librado Carrasco, Jaime Gomez-Laguna, Belen Huerta Lorenzo, Inmaculada Luque
Summary: A cross-sectional study evaluated the diagnostic value of multiplex real-time PCR on faecal and intestinal samples for bovine-paratuberculosis. The study found that faecal PCR had moderate diagnostic value for confirming MAP infection, while PCR on ileocaecal valve samples showed good diagnostic accuracy for post-mortem diagnosis. The combination of both faecal and tissue PCR techniques can help monitor the disease, with the interpretation of results depending on the epidemiological situation.
RESEARCH IN VETERINARY SCIENCE
(2023)
Article
Microbiology
Pablo Rodriguez-Hernandez, Maria Jose Cardador, Rocio Rios-Reina, Jose Maria Sanchez-Carvajal, Angela Galan-Relano, Francisco Jurado-Martos, Inmaculada Luque, Lourdes Arce, Jaime Gomez-Laguna, Vicente Rodriguez-Estevez
Summary: This study evaluates the potential of gas chromatography coupled to ion mobility spectrometry (GC-IMS) in discriminating cattle infected by Mycobacterium tuberculosis complex (MTC) from non-infected animals. Volatile organic compounds (VOCs) produced from feces were analyzed and chemometrics were used to process the data. The results demonstrate that this approach has a robust performance in identifying the infection and non-infection status with high accuracy and sensitivity.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Pablo Rodriguez-Hernandez, Maria Jose Cardador, Rocio Rios-Reina, Jose Maria Sanchez-Carvajal, Angela Galan-Relano, Francisco Jurado-Martos, Inmaculada Luque, Lourdes Arce, Jaime Gomez-Laguna, Vicente Rodriguez-Estevez
Summary: Bovine tuberculosis is a re-emerging disease that poses risks to the livestock sector and public health. Current diagnostic procedures have drawbacks, and alternative tools are needed. This study evaluates the potential of gas chromatography coupled to ion mobility spectrometry (GC-IMS) in distinguishing naturally infected cattle from non-infected animals.
MICROBIOLOGY SPECTRUM
(2023)