Article
Microbiology
Mayo Yasugi, Shingo Hatoya, Daisuke Motooka, Daisuke Kondo, Hideo Akiyoshi, Masayuki Horie, Shota Nakamura, Terumasa Shimada
Summary: The presence of mcr plasmid-mediated colistin-resistant ESBL-producing Enterobacterales in companion dogs and cats in Japan was identified. The isolates from a dog and a cat carried different mcr genes and bla genes on plasmids. This suggests that companion dogs and cats may serve as important reservoirs for cross-species transmission of the mcr gene.
VETERINARY MICROBIOLOGY
(2023)
Article
Infectious Diseases
Antonio Mandujano, Diana Veronica Cortes-Espinosa, Jose Vasquez-Villanueva, Paulina Guel, Gildardo Rivera, Karina Juarez-Rendon, Wendy Lizeth Cruz-Pulido, Guadalupe Aguilera-Arreola, Abraham Guerrero, Virgilio Bocanegra-Garcia, Ana Veronica Martinez-Vazquez
Summary: This study investigated the antimicrobial resistance profile of ESBL-producing E. coli among food-producing animals in Tamaulipas, Mexico. A total of 200 fecal samples were collected and 5.0% of the strains were confirmed as ESBL producers. High percentage of antimicrobial resistance was observed against gentamicin, tetracycline, and streptomycin. The study suggests that food-producing animals may serve as reservoirs and contribute to the spread of ESBL-producing bacteria.
Article
Infectious Diseases
Jiayu Shao, Hangdong Dai, Liwei Xu, Shuilong Zhu, Jufang Zhu, Hangyu Fu, Minxia Ge, Xianhong He
Summary: The purpose of this study was to investigate the genomic characteristics and antimicrobial resistance mechanisms of ESBL-producing E. coli isolates recovered from a district hospital in China. The results showed that these isolates exhibited resistance to multiple antibiotics, with the majority belonging to the ST131 serotype and having different virulence genes and sequence types. Therefore, continuous surveillance and control of the transmission of these multi-drug resistant bacteria in clinical and community settings are crucial.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Immunology
Mabel Kamweli Aworh, Eme Ekeng, Pernille Nilsson, Beverly Egyir, Christian Owusu-Nyantakyi, Rene S. Hendriksen
Summary: This study investigated the zoonotic transmission of extended-spectrum beta-lactamase-producing E. coli (ESBL-EC) among humans, beef cattle, and abattoir environments in Nigeria. The study found a high prevalence of ESBL-EC in beef cattle and abattoir workers, highlighting the importance of genomic surveillance and raising awareness among healthcare workers.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Michel Baez, Ivette Espinosa, Alexandra Collaud, Iliana Miranda, Damarys de las Nieves Montano, Angel L. Feria, Rosa Elena Hernandez-Fillor, Dasiel Obregon, Pastor Alfonso, Vincent Perreten
Summary: This study found an increasing number of third-generation cephalosporin-resistant Escherichia coli (3GC-R-Ec) in poultry from seven farms in the Cuban province of Mayabeque. The isolates were found to contain multiple resistance genes, belong to various sequence types and phylogroups, revealing a genetically diverse population of multiresistant ESBL-producing E. coli in Cuban poultry farms.
Article
Multidisciplinary Sciences
Maria Muddassir, Almas Raza, Sadaf Munir, Ahmad Basirat, Muddassir Ahmed, Mazia Shahid Butt, Omair Arshad Dar, Syed Shoaib Ahmed, Saba Shamim, Syed Zeeshan Haider Naqvi
Summary: This study investigated the antibiogram and drug resistance genes of carbapenem-resistant Pseudomonas aeruginosa, as well as the potency of Silver Nanoparticles against multidrug-resistant strains. The results showed that the conjugated effect of silver nanoparticles with antibiotics effectively inhibited multidrug-resistant Pseudomonas aeruginosa.
SCIENTIFIC REPORTS
(2022)
Article
Infectious Diseases
Toyotaka Sato, Shin-ichi Yokota, Tooru Tachibana, Satoshi Tamai, Shigeki Maetani, Yutaka Tamura, Motohiro Horiuchi
Summary: The study found that companion animals can carry fluoroquinolone-resistant Escherichia coli, which may be associated with human clinical infections and can potentially be transmitted to their owners.
Article
Biochemistry & Molecular Biology
Harpreet Kaur, Vinay Modgil, Naveen Chaudhary, Balvinder Mohan, Neelam Taneja
Summary: Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) and their extended-spectrum beta-lactamase (ESBL) production pose a significant threat to current antimicrobial treatment options. To overcome this challenge, the identification of new therapeutic targets is crucial. In this study, we used computational tools to identify potential drug targets against the ESBL-producing UPEC strain NA114, focusing on chokepoint, virulence, and resistance genes. Through a hierarchical in silico technique, we discovered seven distinct targets for this pathogen that showed no similarity to the human proteome. These targets, involved in bacterial peptidoglycan biosynthesis and inositol phosphate metabolism, hold promise for the design of effective drugs against MDR UPEC.
Article
Microbiology
Joao Pires, Laura Huber, Rachel A. Hickman, Simon Dellicour, Kamonwan Lunha, Thongpan Leangapichart, Jatesada Jiwakanon, Ulf Magnusson, Marianne Sunde, Josef D. Jarhult, Thomas P. Van Boeckel
Summary: Thailand is experiencing differences in antimicrobial use and the composition of antimicrobial resistance genes (ARGs) between small and medium farms. Pig isolates from small farms are more associated with genes conferring resistance to colistin and fluoroquinolones, while pig isolates from medium farms are associated with genes conferring resistance to drugs commonly used on medium farms. ARGs conferring resistance to critically important antimicrobials are more frequently co-carried on plasmids from small farms compared to plasmids from medium farms. Actions to curb the spread of these ARGs are urgently needed.
Article
Multidisciplinary Sciences
Mayo Yasugi, Shingo Hatoya, Daisuke Motooka, Yuki Matsumoto, Shunsuke Shimamura, Hiroyuki Tani, Masaru Furuya, Keiichiro Mie, Masami Miyake, Shota Nakamura, Terumasa Shimada
Summary: This study suggests that companion dogs can spread ESBL/AmpC-producing ExPEC through their feces, with some strains and plasmids potentially being transmitted between humans and companion dogs. Therefore, companion dogs may serve as an important reservoir for ESBL/AmpC-producing E. coli in the community.
Article
Engineering, Environmental
Aradhana Basu, Ananyo Jyoti Misra, Meerambika Behera, Susanta Kumar Behera, Ashish Kumar Nayak, Nabin Kumar Dhal, Amrita Mishra, Bijoy Kumar Satpathy, Cecilia Stalsby Lundborg, Suraj K. Tripathy
Summary: The study demonstrates successful photocatalytic disinfection of MDR Escherichia coli using Alumina/ZnO heterostructures. The proposed technique shows efficacy in disinfecting various bacteria and exhibits biocompatibility towards animal models.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Food Science & Technology
Seyda Sahin, Mahmut Niyazi Mogulkoc, Cemil Kurekci
Summary: This study found that extended spectrum beta-lactamase producing Escherichia coli isolates from chicken meat showed resistance to disinfectants and heavy metals, and carried resistance genes. This poses challenges for disinfection in the poultry industry in Turkey.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Microbiology
Khuliso Ramaite, Mutshiene Deogratias Ekwanzala, Maggy Ndombo Benteke Momba
Summary: ESBL-producing STEC from a cattle farm can contaminate the water environment, posing a potential threat to public health. The study highlighted the importance of continuous surveillance and control measures to prevent STEC-related illnesses originating from livestock environments.
Article
Infectious Diseases
Shazad Mushtaq, Anna Vickers, Nicholas Ellaby, Neil Woodford, David M. Livermore
Summary: The study found that the risk of mutational resistance to aztreonam/avibactam appears smaller than for ceftazidime/avibactam. Asn346 substitutions in AmpC enzymes may remain a risk for resistance.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Jolinda de Korne-Elenbaas, Boas C. L. van der Putten, Niels D. M. Boek, Amy Matser, Constance Schultsz, Sylvia M. Bruisten, Alje P. van Dam
Summary: This study investigates the prevalence and transmission of ESBL-Ec strains among MSM in Amsterdam. It identifies unique ESBL-Ec strains involved in putative transmission between participants, suggesting a high probability of ESBL-Ec transmission in this population.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Immunology
Karina-Doris Vihta, Koen B. Pouwels, Tim E. A. Peto, Emma Pritchard, David W. Eyre, Thomas House, Owen Gethings, Ruth Studley, Emma Rourke, Duncan Cook, Ian Diamond, Derrick Crook, Philippa C. Matthews, Nicole Stoesser, Ann Sarah Walker
Summary: This study compared the ability of different symptoms to identify SARS-CoV-2 infection at specific time points and by vaccination status. The findings suggest that expanding symptom combinations can improve the sensitivity of PCR testing, but it also increases the number of tests per case. Therefore, when implementing large-scale testing strategies, various factors need to be taken into consideration.
CLINICAL INFECTIOUS DISEASES
(2022)
Review
Environmental Sciences
K. K. Chau, L. Barker, E. P. Budgell, K. D. Vihta, N. Sims, B. Kasprzyk-Hordern, E. Harriss, D. W. Crook, D. S. Read, A. S. Walker, N. Stoesser
Summary: This systematic review examined studies using wastewater for antimicrobial resistance (AMR) surveillance and found a high overall concordance between wastewater and human AMR prevalence estimates despite diverse approaches. Future research could benefit from time/location-matched sampling and longitudinal studies to identify optimal practices.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Siyuan Feng, Zhuoxing Wu, Wanfei Liang, Xin Zhang, Xiujuan Cai, Jiachen Li, Lujie Liang, Daixi Lin, Nicole Stoesser, Yohei Doi, Lan-Lan Zhong, Yan Liu, Yong Xia, Min Dai, Liyan Zhang, Xiaoshu Chen, Jian-Rong Yang, Guo-Bao Tian
Summary: The study predicts the evolutionary trajectories of antibiotic resistance genes using a combination of laboratory approaches and deep learning models, providing valuable insights for designing sustainable treatment strategies.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Immunology
Karina Doris Vihta, Koen B. Pouwels, Tim Ea Peto, Emma Pritchard, Thomas House, Ruth Studley, Emma Rourke, Duncan Cook, Ian Diamond, Derrick Crook, David A. Clifton, Philippa C. Matthews, Nicole Stoesser, David W. Eyre, Ann Sarah Walkerand
Summary: A UK community study found that loss of taste/smell was less common in Omicron BA.1/BA.2 infections compared to Delta severe acute respiratory syndrome coronavirus 2 infections. There were smaller declines in reported shortness of breath, myalgia, and fatigue/weakness, but increases in sore throat, challenging symptom-based testing algorithms.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Jia Wei, Philippa C. Matthews, Nicole Stoesser, Ian Diamond, Ruth Studley, Emma Rourke, Duncan Cook, John Bell, John N. Newton, Jeremy Farrar, Alison Howarth, Brian D. Marsden, Sarah Hoosdally, E. Yvonne Jones, David Stuart, Derrick W. Crook, Tim E. A. Peto, A. Sarah Walker, David W. Eyre, Koen B. Pouwels
Summary: Individuals who have previously been infected with SARS-CoV-2 and receive a single dose of vaccine generate similar antibody responses to those who have not been infected and receive two doses of vaccine. Prior infection significantly enhances antibody responses, resulting in higher peak levels and/or longer half-lives after one dose of any of the three vaccines. In those with prior infection, the median time above the positivity threshold was over 1 year after the first vaccination. Single-dose vaccination targeted to those previously infected may provide at least equivalent protection to two-dose vaccination among those without previous infection.
NATURE COMMUNICATIONS
(2022)
Article
Biology
Lele Zhao, Matthew Hall, Mariateresa de Cesare, George MacIntyre-Cockett, Katrina Lythgoe, Christophe Fraser, David Bonsall, Tanya Golubchik, Luca Ferretti
Summary: The raw material for viral evolution comes from intra-host mutations during replication, transcription, or post-transcription. This study explores the mutational spectrum of SARS-CoV-2 and reveals differences in mutation patterns between the negative and positive strands, potentially influenced by host factors.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Hematology
Chanice Knight, Julien Andreani, Neil Garrett, Mark Winter, Tanya Golubchik, Judy Breuer, Claire Reynolds, Susan R. Brailsford, Heli Harvala, Peter Simmonds
Summary: A large outbreak of monkeypox, mainly among men who have sex with men, occurred worldwide from May 2022. This study evaluated the presence of the causative agent, monkeypox virus (MPXV), in blood donations collected in Southern England during the outbreak in August 2022. The results showed no detection of MPXV in the blood donations, indicating a low risk of virus transmission among blood donors during the outbreak.
Review
Medicine, General & Internal
Kasim Allel, Jennifer Stone, Eduardo A. Undurraga, Lucy Day, Catrin E. Moore, Leesa Lin, Luis Furuya-Kanamori, Laith Yakob
Summary: This study quantifies the excess mortality, length of hospital stay, ICU admission, and economic costs associated with antibiotic-resistant bacteria bloodstream infections (ARB BSIs) compared to antibiotic-sensitive bacteria (ASB) among adult inpatients in low- and middle-income countries (LMICs). The findings show that ARB BSIs are associated with higher mortality, longer hospital stays, ICU admissions, and increased economic costs.
Article
Immunology
Piero Olliaro, Juvenal Nkeramahame, Olawale Salami, Catrin E. Moore, Philip Horgan, Rita Baiden, Vida Kukula, Alexander Adjei, James Kapisi, Heidi Hopkins, David Kaawa-Mafigiri, Deborah Ekusai-Sebatta, Elizeus Rutebemberwa, Freddy Eric Kitutu, Halidou Tinto, Francois Kiemde, Adelaide Compaore, Daniel Valia, Sabine Dittrich, DIP study grp
Summary: We introduce the Antimicrobial Resistance Diagnostic Use Accelerator program and the articles in this Supplement that discuss the program in three sub-Saharan African countries.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Immunology
Francois Kiemde, Daniel Valia, Berenger Kabore, Toussaint Rouamba, Alima Nadine Kone, Seydou Sawadogo, Adelaide Compaore, Olawale Salami, Philip Horgan, Catrin E. Moore, Sabine Dittrich, Juvenal Nkeramahame, Piero Olliaro, Halidou Tinto
Summary: This paper reports the primary findings of a randomized controlled trial evaluating the impact of point-of-care tests combined with a training-and-communication package on reducing antibiotic prescriptions for fever management in Burkina Faso. The results show that the implementation of this package can effectively reduce inappropriate antibiotic prescriptions without compromising clinical outcomes.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Immunology
Alexander Adjei, Vida Kukula, Clement Tetteh Narh, Selase Odopey, Emmanuel Arthur, Gabriel Odonkor, Michael Matey Mensah, Piero Olliaro, Philip Horgan, Sabine Dittrich, Catrin E. Moore, Olawale Salami, Elizabeth Awini, Juvenal Nkeramahame, John Williams, Rita Baiden
Summary: This study aimed to evaluate the impact of a package of interventions on antibiotic prescribing and clinical outcomes for patients with acute febrile illness in Ghana. The results showed that the use of rapid diagnostic tests and diagnostic algorithms can significantly reduce antibiotic prescriptions and improve clinical outcomes for children and patients with nonmalarial fevers and respiratory symptoms.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Immunology
Piero Olliaro, Juvenal Nkeramahame, Philip Horgan, Halidou Tinto, Francois Kiemde, Rita Baiden, Alexander Adjei, James Kapisi, Heidi Hopkins, Olawale Salami, Catrin E. Moore, Sabine Dittrich, Stephan Weber, Stefano Ongarello, ADIP Study Grp
Summary: This meta-analysis included 3 randomized trials conducted in sub-Saharan Africa comparing the effects of point-of-care tests and diagnostic algorithms versus routine care on antibiotic prescriptions and clinical outcomes in ambulatory patients presenting at outpatient facilities with acute uncomplicated febrile illness.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Immunology
James Kapisi, Asadu Sserwanga, Freddy Eric Kitutu, Elizeus Rutebemberwa, Phyllis Awor, Stephan Weber, Thomas Keller, David Kaawa-Mafigiri, Deborah Ekusai-Sebatta, Philip Horgan, Sabine Dittrich, Catrin E. Moore, Olawale Salami, Piero Olliaro, Juvenal Nkeramahame, Heidi Hopkins
Summary: Introducing a package of diagnostic tools, clinical algorithm, and training-and-communication messages did not significantly reduce antibiotic prescribing for febrile outpatients in Uganda.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Medicine, General & Internal
Zikria Saleem, Abdul Haseeb, Safa S. Almarzoky Abuhussain, Catrin E. E. Moore, Sairah Hafeez Kamran, Muhammad Usman Qamar, Aisha Azmat, Giuseppe Pichierri, Fahad Raees, Shahzad Asghar, Amna Saeed, Afreenish Amir, Furqan Khurshid Hashmi, Johanna C. C. Meyer, Israel Abebrese Sefah, Inaam Ur Rehman, Muhammad Umer Nadeem, Brian Godman
Summary: This study aimed to monitor the susceptibility pattern of microbes in Pakistan and found that the current antimicrobial options are severely restricted due to the emergence of multidrug-resistant pathogens. Urgent actions, including initiating antimicrobial stewardship programs, are needed to enhance prudent prescribing of antibiotics.
MEDICINA-LITHUANIA
(2023)
Article
Environmental Sciences
Kasim Allel, Lucy Day, Alisa Hamilton, Leesa Lin, Luis Furuya-Kanamori, Catrin E. Moore, Thomas Van Boeckel, Ramanan Laxminarayan, Laith Yakob
Summary: This study assessed the associations between socioeconomic, anthropogenic, and environmental indicators and country-level rates of antimicrobial resistance (AMR) in humans and food-producing animals. The results showed that there were significant associations between animal antimicrobial consumption and AMR in food-producing animals, as well as between human antimicrobial consumption and AMR in WHO priority pathogens. Bidirectional associations were also found between animal antibiotic consumption and resistance in critical priority human pathogens, and between human antibiotic consumption and animal AMR. This study suggests that reducing antibiotic consumption alone is not enough to combat the rising prevalence of AMR, and control methods should focus on poverty reduction and preventing AMR transmission across different domains.
LANCET PLANETARY HEALTH
(2023)