Article
Plant Sciences
Jianan Han, Shaoyun Dong, Yanxia Shi, Zhuonan Dai, Han Miao, Baoju Li, Xingfang Gu, Shengping Zhang
Summary: In this study, the GSB resistance of cucumber core germplasms from four geographic groups was investigated at the seedling and adult stages. Multiple SNPs related to GSB resistance were identified, some of which co-localized with previously mapped QTLs. Haplotype analysis and expression levels after inoculation led to the identification of four candidate genes. These findings contribute to the breeding of GSB resistant cucumber cultivars and provide insights into GSB resistance mechanism.
HORTICULTURAL PLANT JOURNAL
(2023)
Article
Plant Sciences
Qing Meng, Zhiyuan Liu, Chunda Feng, Helong Zhang, Zhaosheng Xu, Xiaowu Wang, Jian Wu, Hongbing She, Wei Qian
Summary: In this study, a major quantitative trait locus (QTL) related to early bolting in spinach was identified, and two candidate genes associated with flowering regulatory pathways were proposed. This research provides a basis for understanding the molecular mechanisms of bolting in spinach.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Jia Wang, Lin Mao, Zhaoqiong Zeng, Xiaobo Yu, Jianqiu Lian, Jun Feng, Wenying Yang, Jiangang An, Haiying Wu, Mingrong Zhang, Liezhao Liu
Summary: This study utilized QTL mapping, QTL-seq, and RNA sequencing to identify genes controlling protein content in soybean using the Nanxiadou 25 variety. Major QTLs on chromosome 20 were repeatedly detected across multiple environments, indicating potential candidate genes. Through gene expression analysis and clone sequencing, different SNPs and indels were identified as potentially responsible for changes in protein content.
Article
Multidisciplinary Sciences
Lili Du, Tianpeng Chang, Bingxing An, Mang Liang, Xinghai Duan, Wentao Cai, Bo Zhu, Xue Gao, Yan Chen, Lingyang Xu, Lupei Zhang, Junya Li, Huijiang Gao
Summary: This study investigated the molecular mechanism underlying water holding capacity (WHC) in Chinese Simmental beef cattle by conducting RNA sequencing on longissimus dorsi (LD) muscles. Significant correlations were found between WHC and water loss, IMF content, and specific genes were identified as potential novel candidate genes affecting WHC. The findings offer important insight for understanding the regulation of WHC and improving beef quality.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Shuiyuan Hao, Hongyao Lou, Haiwei Wang, Jinghong Shi, Dan Liu, Baogerile, Jianguang Tao, Sanming Miao, Qunce Pei, Liangliang Yu, Min Wu, Ming Gao, Naihu Zhao, Jinchao Dong, Mingshan You, Mingming Xin
Summary: This study investigated the genetic loci associated with five quality traits in Chinese wheat grown in Inner Mongolia through genome wide association mapping. Multiple QTLs were found to be associated with various traits, and the distribution of favorable alleles of stable QTLs was validated. The study provides novel insights into the genetic basis of quality traits in wheat.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Tengkui Chen, Lixin Luo, Zhe Zhao, Hui Wang, Chun Chen, Yongzhu Liu, Xinchang Li, Tao Guo, Wuming Xiao
Summary: Grain size affects rice yield and quality. This study constructed a near-isogenic line LB3 with long grain size and identified a major QTL, qGL10, located on chromosome 10, for grain length. Candidate gene analysis suggested LOC_Os10g39130 (OsMADS56). Sequence comparison revealed SNPs in the promoter and coding regions of OsMADS56 in 02428 and LB3. Haplotype analysis indicated that the SNP9 (G/A) in the TGTCACA motif may contribute to the differential expression of OsMADS56. These findings provide a foundation for the molecular breeding of new rice varieties using qGL10.
Article
Plant Sciences
Ian S. E. Bally, Aureliano Bombarely, Alan H. Chambers, Yuval Cohen, Natalie L. Dillon, David J. Innes, Maria A. Islas-Osuna, David N. Kuhn, Lukas A. Mueller, Ron Ophir, Aditi Rambani, Amir Sherman, Haidong Yan
Summary: The study sequenced, assembled, and analyzed the genome of the monoembryonic mango cultivar 'Tommy Atkins', identifying candidate genes associated with fruit size characteristics and a large number of SNPs. The availability of the 'Tommy Atkins' mango genome will significantly impact global initiatives in mango genetics research.
Article
Fisheries
Yu Zhou, Huang-Cui Fu, Ying-Ying Wang, He-Zhong Huang
Summary: In this study, a genome-wide association study (GWAS) was performed using mandarin fish to identify SNPs and candidate genes associated with growth traits. Many of the candidate genes were found to be related to bone differentiation, growth and development, cell division, and neurogenesis.
Article
Cell & Tissue Engineering
Katya L. Mack, Heather E. Talbott, Michelle F. Griffin, Jennifer B. L. Parker, Nicholas J. Guardino, Amanda F. Spielman, Michael F. Davitt, Shamik Mascharak, Mauricio Downer, Annah Morgan, Caleb Valencia, Deena Akras, Mark J. Berger, Derrick C. Wan, Hunter B. Fraser, Michael T. Longaker
Summary: In adult mammals, skin wounds typically heal through scarring. However, the MRL mice have the ability to regenerate ear punch wounds. The researchers identified the molecular basis for this regeneration by analyzing gene expression in hybrid crosses between MRL and non-regenerating mice. They found that regulatory differences in fibroblasts were associated with wound-healing pathways and treatment with one of these proteins accelerated wound repair and induced regeneration in fibrotic wounds. The study provides insights into the molecular drivers of regeneration with potential clinical implications.
Article
Agronomy
Yuheng Wu, Chenyu He, Changheng Sun, Xiangran Wang, Zhaoming Qi, Qingshan Chen, Mingzhe Zhao, Xindong Yao, Dayong Zhang
Summary: Understanding the genetic basis of leaf sucrose content can lead to improved soybean yields. This study used 190 chromosome fragment substitution lines (CSSLs) to identify related QTLs. Only one QTL with a high LOD score was detected among 3780 bin markers distributed in 20 chromosomes. Nine candidate genes were screened, and Glyma.14G029100 was identified as the key gene. Differences in the promoter and CDS sequences were found, leading to differences in relative transcriptional levels. These findings lay the groundwork for further research into the genetic mechanism of leaf sucrose content in soybeans.
Article
Plant Sciences
Jacob R. MacWilliams, Paul D. Nabity, Kerry E. Mauck, Isgouhi Kaloshian
Summary: This study investigates the interaction between cowpea plants and aphids and identifies the genes underlying cowpea resistance to aphids. The results show that the feeding behavior of aphids remodels the transcriptome of cowpea and that this remodeling depends on both the duration of feeding and host-plant resistance. The study also identifies candidate genes involved in aphid resistance, including both conventional resistance genes and novel genes.
Article
Biotechnology & Applied Microbiology
Fei Zhang, Deborah Velez-Irizarry, Catherine W. Ernst, Wen Huang
Summary: In this study, genome-wide analysis of alternative splicing in skeletal muscle of a Duroc x Pietrain F2 pig population was performed. It was found that the heritability of alternative splicing scores was lower than that of overall gene expression. Additionally, there was little correlation between alternative splicing and overall gene expression. Integration of splice QTL mapping with phenotype QTL mapping identified potential mediators of phenotype QTL effects through alternative splicing.
Article
Genetics & Heredity
Nagenahalli Dharmegowda Rathan, Deepmala Sehgal, Karthikeyan Thiyagarajan, Ravi Singh, Anju-Mahendru Singh, Velu Govindan
Summary: The study on nutritionally enhanced wheat identified new genomic regions controlling grain iron and zinc concentrations, leading to the discovery of pleiotropic QTL that could be used in wheat biofortification breeding to address micronutrient deficiency among wheat consumers. The in silico analysis revealed candidate genes involved in transporting small peptides and minerals as well as catalyzing phosphorylation processes, providing insights into the mechanisms of mineral uptake in wheat.
FRONTIERS IN GENETICS
(2021)
Article
Agronomy
Gurkamal Kaur, Parneet K. Toora, Pham Anh Tuan, Curt A. Mccartney, Marta S. Izydorczyk, Ana Badea, Belay T. Ayele
Summary: Genome-wide association study identified loci, single nucleotide polymorphisms, and candidate genes that control seed dormancy and enhance resistance to preharvest sprouting in diverse barley genotypes. Significant variation in germination index and dormancy levels among the genotypes was observed. SNPs on chromosome 3H and 5H and their corresponding candidate genes, including HvRCD1, HvPSRP1, and HvF3H, were found to play important roles in controlling seed dormancy and preharvest sprouting resistance.
THEORETICAL AND APPLIED GENETICS
(2023)
Article
Plant Sciences
Carmen Santos, Davide Coelho Martins, Maria Jose Gonzalez-Bernal, Diego Rubiales, Maria Carlota Vaz Patto
Summary: In this study, a combination of phenotyping, gene expression, and linkage mapping was used to prioritize four candidate genes relevant for disease-resistance precision breeding in Lathyrus cicera. These genes are involved in adaptation to biotic stress, cellular and organelle homeostasis, and proteins directly involved in plant defense.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Genetics & Heredity
Ibrahim Abousoliman, Henry Reyer, Michael Oster, Eduard Murani, Ismail Mohamed, Klaus Wimmers
Summary: Sheep play a critical role in Egypt's agricultural and livestock sectors. The Barki sheep breed, known for its adaptation to harsh environmental conditions, contributes significantly to meat production in semi-arid regions. A study using a genome-wide SNP panel identified genetic regions diversified between Egyptian Barki sheep with high and low growth performance traits, highlighting potential for improving growth traits in this breed.
Article
Agriculture, Dairy & Animal Science
Ibrahim Abousoliman, Henry Reyer, Michael Oster, Eduard Murani, Ismail Mohamed, Klaus Wimmers
Summary: This study utilized rare milk performance data and genomic information of Barki sheep to identify genomic regions associated with milk yield and quality traits, offering valuable insights for genetic improvement in this breed. Genes such as SLC5A8, NUB1, TBC1D1, KLF3, ABHD5, PPARA, and FBLN1 were found to be related to milk performance traits in Barki sheep.
Article
Agriculture, Dairy & Animal Science
Cai Chen, Yao Zheng, Mengli Wang, Eduard Murani, Enrico D'Alessandro, Ali Shoaib Moawad, Xiaoyan Wang, Klaus Wimmers, Chengyi Song
Summary: Retrotransposon insertion polymorphisms (RIPs) play a significant role in the genetic variation of GH/IGF axis genes, with one SINE RIP in the intron 1 of GHR potentially acting as a repressor to decrease GHR expression.
Article
Microbiology
Henry Reyer, Per J. R. Sjoberg, Michael Oster, Aisanjiang Wubuli, Eduard Murani, Siriluck Ponsuksili, Petra Wolf, Klaus Wimmers
Summary: The manipulation of intestinal microbiota through feeding measures offers possibilities for optimizing the utilization of intestinal phytate and phosphorus, which is essential for proper growth processes and bone stability in pigs in livestock farming.
Article
Agronomy
Dario Fornara, Elizabeth M. E. Ball, Christina Mulvenna, Henry Reyer, Michael Oster, Klaus Wimmers, Hanne Damgaard Poulsen, Arno Rosemarin
Summary: The over-supplementation of animal feeds with phosphorus leads to high rates of P excretion and negative environmental impacts. Adding phytase to pig and poultry diets can reduce P excretion, but the effects on plant-soil systems are poorly understood. This study found that phytase supplementation significantly influenced P content of animal waste and plant P dynamics, with intermediate phytase levels contributing to higher P use efficiency and lower soil P availability. The effects of dietary P content on P losses from soils varied depending on the type of organic amendment.
Article
Agriculture, Dairy & Animal Science
Christina C. Mulvenna, Ursula M. McCormack, Elizabeth Magowan, John McKillen, Mike R. Bedford, Carrie L. Walk, Michael Oster, Henry Reyer, Klaus Wimmers, Dario A. Fornara, M. Elizabeth E. Ball
Summary: Phytase has been a common addition to poultry diets for over 20 years, showing benefits in growth performance, nutrient digestibility, and bone parameters. This study found that superdosing phytase in rapeseed meal and maize dried distiller grains with solubles diets can improve performance, increase digestibility, and reduce P excretion by 15% compared to birds without phytase.
Article
Biochemistry & Molecular Biology
Maruf Hasan, Michael Oster, Henry Reyer, Siriluck Ponsuksili, Eduard Murani, Petra Wolf, Dagmar-Christiane Fischer, Klaus Wimmers
Summary: This study investigated the tissue-wide expression patterns of key genes related to vitamin D metabolism and FGF23 signaling in pigs fed different phosphorus diets. The results showed that non-renal tissues and cells have specific requirements for active vitamin D formation. Low phosphorus diet suppressed vitamin D signaling locally, while high phosphorus diet stimulated local vitamin D synthesis and regulation of FGF23 signaling in bone.
Article
Agriculture, Dairy & Animal Science
Adewunmi O. Omotoso, Henry Reyer, Michael Oster, Steffen Maak, Siriluck Ponsuksili, Klaus Wimmers
Summary: Phosphorus inclusion in broiler diets needs to be tailored to their physiological demands at different developmental stages. A timed nutritional conditioning strategy, such as P depletion, may enhance mineral homeostasis mechanisms and reduce dietary P supply. This study investigated the effects of dietary P depletion on broiler chickens at different developmental stages, revealing marked responses early on, but indications of compensatory mechanisms later. Endocrine control mediated by calcitriol, intestinal P uptake, and mineral mobilization from bones were identified as potential mechanisms for maintaining mineral homeostasis. Precise timing, duration, and extent of P depletion should be considered for optimized nutrient utilization in broiler husbandry.
Article
Chemistry, Analytical
Mohd. Rahil Hasan, Pradakshina Sharma, Shifa Shaikh, Saumitra Singh, Roberto Pilloton, Jagriti Narang
Summary: We have developed a paper-based experimental platform for analyzing dengue virus, which incorporates screen-printing technology and black carbon conductive ink. The platform utilizes both two-electrode and three-electrode systems, with an LOD of 0.1 μg/mL for detecting the virus. The three-electrode system exhibits a significantly higher current range and amplification compared to the two-electrode system, making it a viable option for sensitive detection of dengue virus and other diseases.
Article
Nutrition & Dietetics
Maruf Hasan, Michael Oster, Henry Reyer, Klaus Wimmers, Dagmar-Christiane Fischer
Summary: Vitamin D-3 (Vit D-3) and 25(OH)D-3 are used as dietary sources of active vitamin D in pig husbandry, and while their primary effects are on the intestine, kidney, and bone, they also have wide-ranging effects on peripheral tissues. The use of Vit D-3 and 25(OH)D-3 in pig nutrition has been shown to have different outcomes in terms of reproductive capacities, growth performance, immunity, and bone development.
BRITISH JOURNAL OF NUTRITION
(2023)
Correction
Nutrition & Dietetics
Maruf Hasan, Michael Oster, Henry Reyer, Klaus Wimmers, Dagmar-Christiane Fischer
BRITISH JOURNAL OF NUTRITION
(2023)
Article
Agriculture, Dairy & Animal Science
Hanne Honerlagen, Henry Reyer, Ibrahim Abou-Soliman, Dierck Segelke, Siriluck Ponsuksili, Nares Trakooljul, Norbert Reinsch, Bjoern Kuhla, Klaus Wimmers
Summary: Improving the nitrogen-utilization efficiency (NUE) of dairy cows through breeding selection has benefits in terms of nutrition, environment, and economics. The individual milk urea concentration (MU) of cows has been proposed as an indicator trait for NUE. This study aimed to identify different microbial genera in the rumen of Holstein cows with divergent genomic breeding values for MU (GBVMU). The identified microbial genera were further investigated for their correlations with MU and other NUE-associated traits, providing insight into genetically determined N-utilization in Holstein cows.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Cell Biology
Julia Brenmoehl, Elli Brosig, Nares Trakooljul, Christina Walz, Daniela Ohde, Antonia Noce, Michael Walz, Martina Langhammer, Stefan Petkov, Monika Roentgen, Steffen Maak, Christina E. E. Galuska, Beate Fuchs, Bjoern Kuhla, Siriluck Ponsuksili, Klaus Wimmers, Andreas Hoeflich
Summary: This study investigated the contribution of non-mitochondrial and mitochondrial metabolic pathways in contracting muscles during repeated training. The results showed that marathon mice displayed increased glucose and fatty acid oxidation, reduced lactate dehydrogenase activity, and inhibited lactate formation compared to trained control mice. This suggests that marathon mice have enhanced metabolic flexibility towards lipid catabolism regulated by lactate dehydrogenase.
Review
Agriculture, Dairy & Animal Science
Benjamin Diehl, Michael Oster, Andreas Vernunft, Klaus Wimmers, Hartwig Bostedt
Summary: The losses of piglets in commercial pig farming remain a concern and sustainable breeding and management strategies are needed. This review systematically presents the three phases of neonatal adaptation in piglets and highlights the key developments and influencing factors in each phase.
ARCHIVES ANIMAL BREEDING
(2022)
Review
Biochemistry & Molecular Biology
Fabio Sarais, Alvaro Perdomo-Sabogal, Klaus Wimmers, Siriluck Ponsuksili
Summary: This review focuses on the latest discoveries of tsRNAs, with special attention to molecules initially thought to be associated with tiRNAs. The paper outlines their biological functions, provides a collection of useful databases, and discusses future research perspectives and applications in livestock basic and applied research.