Article
Biotechnology & Applied Microbiology
Lingzhan Xue, Yu Gao, Meiying Wu, Tian Tian, Haiping Fan, Yongji Huang, Zhen Huang, Dapeng Li, Luohao Xu
Summary: The study on the haplotype-resolved genome assembly of zig-zag eel sheds light on the evolution of sex chromosomes and recombination suppression mechanisms, revealing a similar sex-linked region on the X and Y chromosomes and identifying a potential sex-determining gene in the SLR.
Article
Plant Sciences
Qinzheng Zhao, Ya Meng, Panqiao Wang, Xiaodong Qin, Chunyan Cheng, Junguo Zhou, Xiaqing Yu, Ji Li, Qunfeng Lou, Molly Jahn, Jinfeng Chen
Summary: A new method was developed to rapidly and accurately represent karyotypes of Cucumis species, revealing that African and Asian origin species underwent large-scale genomic reshuffling during evolution.
Review
Cell Biology
Brandt Warecki, William Sullivan
Summary: A conserved feature of higher eukaryotes is that centromeres are embedded in heterochromatin. This tight association is essential for proper cell division and nuclear envelope formation.
Review
Biochemistry & Molecular Biology
Marcelo Ricardo Vicari, Daniel Pacheco Bruschi, Diogo Cavalcanti Cabral-de-Mello, Viviane Nogaroto
Summary: This article revisits the central role of telomeres in chromosomal stability events, discussing their organization, function, and maintenance mechanisms, as well as the involvement of ITS in chromosome changes. While vertebrate telomeres typically consist of a (TTAGGG)(n) sequence, insects show greater diversity in their telomeric motifs. Studies have observed the relationship between ITS and chromosome rearrangements in various species groups, highlighting different types of genome organization and distribution.
GENETICS AND MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Aleksandra O. Travina, Nadya Ilicheva, Alexey G. Mittenberg, Sergey Shabelnikov, Anastasia Kotova, Olga Podgornaya
Summary: TRF2's long linker region udTRF2 may play a role in the interaction between TRF2 and lamin proteins, as well as interacting with other proteins. Mass spectrometry analysis revealed that apart from interacting with lamin proteins, udTRF2 also interacts with a variety of other proteins; some of the known interactors are involved in telomere biology, while the roles of others are yet to be investigated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Manon Poignet, Martina Johnson Pokorna, Marie Altmanova, Zuzana Majtanova, Dmitry Dedukh, Tomas Albrecht, Jiri Reif, Tomasz S. Osiejuk, Radka Reifova
Summary: The chromosomal structures of the common nightingale and thrush nightingale are conservatively similar with just a few subtle changes, mainly in centromeric repetitive sequences. These changes could potentially affect hybrid fertility. Overall, the slow rate of karyotype evolution in birds is reflected in the similar karyotypes of the two nightingale species.
FRONTIERS IN GENETICS
(2021)
Article
Zoology
Vaclav Gvozdik, Martin Knytl, Ange-Ghislain Zassi-Boulou, Nicola R. Fornaini, Barbora Bergelova
Summary: This study uses cytogenetics to investigate the dwarf clawed frogs in the African pipids, revealing that the captive dwarf clawed frogs should be referred to as Hymenochirus sp. instead of the commonly used names 'H. boettgeri' or 'H. curtipes'. The study also identifies the karyotype of H. boettgeri as tetraploid, evolved through chromosome fusion and subsequent allotetraploidization. Additionally, a stable presence of a single B chromosome is observed in the captive population of Hymenochirus sp.
ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY
(2023)
Article
Biotechnology & Applied Microbiology
William Poisson, Julien Prunier, Alexandra Carrier, Isabelle Gilbert, Gabriela Mastromonaco, Vicky Albert, Joelle Taillon, Vincent Bourret, Arnaud Droit, Steeve D. Cote, Claude Robert
Summary: This study used Oligopaint FISH technology to design specific probes and successfully upgraded the published caribou chromosome-level genomes. By combining physical mapping and comparative genomics, the chromosomal evolution among Cervidae and closely related bovids was revealed. These results provide validation for the current chromosome-level genome assembly and resources for chromosome banding studies in caribou.
Article
Biotechnology & Applied Microbiology
Hao Zhang, Fei Wang, Chunyan Zeng, Wei Zhu, Lili Xu, Yi Wang, Jian Zeng, Xing Fan, Lina Sha, Dandan Wu, Yiran Cheng, Haiqin Zhang, Guoyue Chen, Yonghong Zhou, Houyang Kang
Summary: The FISH karyotype established in this study lays a solid foundation for the efficient identification of P. huashanica chromosomes in wheat genetic improvement programs.
Article
Horticulture
Yan He, Lulu Yang, Yanjun Zhang, Qiong Liang
Summary: This study provides a comprehensive cytological analysis of the Bletilla, a genus of orchids with high medical value. The study obtained detailed estimates of ploidy level, karyotype, and genome size and found moderate cytological variation in Bletilla. The study also observed differences in karyotype symmetry and genome size among different species.
Article
Genetics & Heredity
Fang Fang, Ru Jia, Congyan Liu, Hong Zhao, Wanling Sun
Summary: This study investigated the genetic and clinical features of a rare t(1;12)(q21;p13) in a patient with myelodysplastic syndrome (MDS), and the results suggested that this chromosomal translocation might have a negative impact on the survival rate of MDS.
MOLECULAR GENETICS & GENOMIC MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Dandan Wu, Namei Yang, Qian Xiang, Mingkun Zhu, Zhongyan Fang, Wen Zheng, Jiale Lu, Lina Sha, Xing Fan, Yiran Cheng, Yi Wang, Houyang Kang, Haiqin Zhang, Yonghong Zhou
Summary: This study investigated the chromosomes of the Pseudoroegneria libanotica and identified its characteristics and intraspecific polymorphism. The findings provide important information for the study of the evolution of the P. libanotica genome. The study also suggested the heterozygosity and adaptation as factors influencing the polymorphism in P. libanotica.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Kira S. Zadesenets, Nikolay B. Rubtsov
Summary: This study focused on the B chromosomes in three species of the Macrostomum genus that underwent recent whole-genome duplication. Analysis of Bs in animals under laboratory conditions revealed differences in Bs among species and the repetitive DNA composition of Bs. Additionally, the possible mechanisms of the appearance and evolution of Bs and large metacentric chromosomes (LMs) in species of the Macrostomum genus were discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agronomy
Padmaja Patel, Subrat Kumar Dehery, Satya Narayan Jena, Chinmay Pradhan, Anath Bandhu Das
Summary: This research reports the cytogenetic and genome size variation of Indian Squill. Different ploidy levels, including diploid, tetraploid, and aneuploid, were observed in different ecotypes. The findings have important implications for breeding improvement, extraction of therapeutic phytoconstituents, and taxonomic identification of the species.
GENETIC RESOURCES AND CROP EVOLUTION
(2023)
Article
Biochemistry & Molecular Biology
Parinaz Kazemi, Teruko Taketo
Summary: It has been found in mouse oocytes that telomeres play a crucial role in chromosome pairing and synapsis, with D-telomeres often initiating synapsis preferentially. The absence of protein SPO11 leads to a higher frequency of synapsis initiation at C-telomeres.
Article
Biology
Dmitrij Dedukh, Alla Krasikova
Summary: Genome stability is crucial for eukaryotic organisms, but some organisms can selectively eliminate part of their genomes during specific developmental stages. Programmed DNA elimination, occurring independently in different organisms, serves as a mechanism for dosage compensation and inactivation of unnecessary or dangerous genetic entities. Genome elimination in hybrids results from competition between parental genomes.
BIOLOGICAL REVIEWS
(2022)
Article
Reproductive Biology
Tomas Tichopad, Roman Franek, Marie Dolezalkova-Kastankova, Dmitrij Dedukh, Anatolie Marta, Karel Halacka, Christoph Steinbach, Karel Janko, Martin Psenicka
Summary: Interspecific hybridization may lead to a transition from sexual reproduction to asexual reproduction, but the reasons for this change are not well understood. In this study, we investigated the triggers of asexual reproduction and found that it is directly influenced by the hybrid genetic constitution of the cell. Additionally, the execution of asexual development is regulated by signals from the surrounding gonadal environment and somatic factors.
BIOLOGY OF REPRODUCTION
(2022)
Article
Biochemistry & Molecular Biology
Manuelita Sotelo-Munoz, Manon Poignet, Tomas Albrecht, Ondrej Kauzal, Dmitrij Dedukh, Stephen A. Schlebusch, Karel Janko, Radka Reifova
Summary: Passerine birds have a unique chromosome called the germline-restricted chromosome (GRC) in their germ cells, which varies greatly in size and composition among species, and may contribute to reproductive isolation and speciation rate in passerine birds.
Article
Developmental Biology
Dmitrij Dedukh, Marie Altmanova, Jiri Klima, Lukas Kratochvil
Summary: Obligate parthenogenesis has evolved multiple times in reptiles through interspecific hybridization, resulting in the formation of diploid and triploid lineages. This study shows that oocytes in several widespread parthenogenetic complexes of geckos undergo meiosis and produce unreduced eggs through premeiotic endoreplication, allowing for the emergence of fertile polyploid lineages within parthenogenetic complexes.
Article
Zoology
Anton O. Svinin, Kseniya A. Matushkina, Dmitry Dedukh, Ivan Bashinskiy, Oleg A. Ermakov, Spartak N. Litvinchuk
Summary: The study found that the anomaly P syndrome can be caused by the trematode Strigea robusta, and polydactyly and heavy forms of the syndrome can also be observed in toads of the genera Bufo and Bufotes. Furthermore, it was discovered that the parasite does not have any effect on the gonadal development of the toads.
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Dmitrij Dedukh, Sergey Riumin, Krzysztof Kolenda, Magdalena Chmielewska, Beata Rozenblut-Koscisty, Mikolaj V. Kazmierczak, Maria V. Ogielska, Alla V. Krasikova
Summary: This study focused on the reproduction and maintenance of diploid and triploid hybrid frogs in natural populations. Cytogenetic analysis revealed variability in gametogenesis pathways and observed a significant role of postzygotic barriers in the survival rate of hybrid progeny.
Article
Zoology
Dmitriyv V. Skorinov, Emin Bozkurt, Kurtulus Olgun, Spartak N. Litvinchuk
Summary: The location, shape and area of dark spots on the bodies of three closely related species of smooth newts (Lissotriton kosswigi, L. schmidtleri and L. vulgaris) were analyzed. Differences in spot characteristics allow for high confidence identification of males and females, with one species accurately distinguishable from the others. Similar patterns of dark spots confirm the conspecificity of Anatolian and Thracian populations of L. schmidtleri. The method developed in this study can be useful for identifying other animal species with spotted camouflage or aposematic coloration.
ACTA ZOOLOGICA ACADEMIAE SCIENTIARUM HUNGARICAE
(2022)
Article
Biochemistry & Molecular Biology
Tatiana Kulikova, Antonina Maslova, Polina Starshova, Juan Sebastian Rodriguez Ramos, Alla Krasikova
Summary: This study compares somatic topologically associating domains (TADs) in chicken embryonic fibroblasts with chromomere-loop complexes in lampbrush meiotic chromosomes. The results suggest that there may be similarities between TADs and chromomere-loop complexes, with multiple TADs potentially being included in one chromomere-loop complex. Additionally, transcription of genes related to oocyte maturation and early embryo development was observed on the lampbrush chromosomes.
Article
Biochemistry & Molecular Biology
Artem Nurislamov, Timofey Lagunov, Maria Gridina, Alla Krasikova, Veniamin Fishman
Summary: DNA methylation is an important epigenetic mechanism involved in various genomic processes. This study investigated DNA methylation patterns in chicken diplotene oocytes and found that they closely resemble the genomic distribution observed in somatic tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Eleonora Pustovalova, Lukas Choleva, Dmytro Shabanov, Dmitrij Dedukh
Summary: This study investigates the mechanism of sperm production in hybrid frogs, finding that they produce sperm with different genome compositions and ploidy levels.
Article
Multidisciplinary Sciences
M. Gridina, A. Taskina, T. Lagunov, A. Nurislamov, T. Kulikova, A. Krasikova, V. Fishman
Summary: Advances in single-cell sequencing technologies have enabled the study of genome architecture in individual cells. However, there are significant differences in efficiency, bias, scale, and cost among different single-cell Hi-C protocols, and their relative advantages for different applications are unclear. By comparing the two most commonly used single-cell Hi-C protocols, researchers can gain guidance.
Review
Biochemistry & Molecular Biology
Alla Krasikova, Veniamin Fishman, Tatiana Kulikova
Summary: Extraordinary lampbrush chromosomes with thousands of transcription loops have significant importance in chromosome biology research. These chromosomes become lampbrushy due to a high rate of transcription during oogenesis. Recent studies have revealed new information on transcribed sequences and the genomic context of individual chromomeres, pushing the boundaries of the discussion on their biological role. Combining genome-wide approaches with microscopy imaging techniques using lampbrush chromosomes as a model object offers fruitful avenues for future research.
Article
Biotechnology & Applied Microbiology
A. Maslova, V. Plotnikov, M. Nuriddinov, M. Gridina, V. Fishman, A. Krasikova
Summary: This study analyzed the 3D genome organization and chromosome rearrangements in the LSCC-HD3 cell line using high-throughput chromosome conformation capture (Hi-C) technology. It revealed a severely rearranged karyotype in the HD3 cell line and provided insights into the genome structure-function relationships in avian models with a large number of microchromosomes.
Article
Biology
Anna Fedorova, Dmytro Shabanov
Summary: This study compares the release calls of different sexes of hybrid Pelophylax esculentus and its parental species in Ukrainian populations. It found that triploid male hybrids are intermediate between diploid hybrids and parental species, while triploid females are only intermediate in terms of temporal parameters. Frequency parameters mainly depend on body size. There is a significant correlation between peak frequency and body size in females of both taxonomical groups and P. ridibundus males.
