Article
Multidisciplinary Sciences
Rikho Jerikho, Surya Gentha Akmal, Veryl Hasan, Yonvitner, Jindrich Novak, Andre Lincoln Barroso Magalhaes, Alberto F. Maceda-Veiga, Michael L. Tlusty, Andrew Rhyne, Ondrej Slavik, Jiri Patoka
Summary: The pet trade serves as an important pathway for the introduction of aquatic non-native species, with Indonesia playing a significant role in this trade. A survey of the Indonesian market and aquaculture reveals the volume of trade and the customer countries involved in the import of South American river stingrays. Analysis of the climate similarities between the native range of these species and Indonesia identifies several areas suitable for their establishment, and the presence of established populations in the Brantas River confirms this. The unregulated culture of these stingrays poses a risk to wildlife, highlighted by the first recorded case of envenomation caused by Potamotrygon spp. outside of South America. Continuous monitoring and risk mitigation measures are strongly recommended.
SCIENTIFIC REPORTS
(2023)
Article
Plant Sciences
Anton Stepanenko, Guimin Chen, Phuong T. N. Hoang, Joerg Fuchs, Ingo Schubert, Nikolai Borisjuk
Summary: This study comprehensively characterized the chromosomal locations and molecular organization of 35S and 5S rDNA genes in water lettuce using karyological and molecular methods. Single locations for the 35S and 5S rDNA loci were identified using fluorescence in situ hybridization. The molecular cloning and nucleotide sequencing of 35S rDNA revealed typical structural characteristics and allowed for phylogenetic analysis. The 5S rDNA locus showed variation in nucleotide composition and subrepeat number, confirming the mosaic composition of the gene locus.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Alexander Dyomin, Svetlana Galkina, Arina Ilina, Elena Gaginskaya
Summary: In the majority of Animalia genomes, the 5S rRNA gene repeats are located outside of the nucleolar organiser (NOR). However, in the family Nototheniidae, a 5S rDNA sequence is inserted into the intergenic spacer between the 45S rDNA repeats. This sequence, termed NOR-5S rRNA gene, is colocalized with the 45S rDNA and is considered a maternal-type 5S rRNA template.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Horticulture
Guangxin Liu, Yue Lan, Lianwei Qu, Yilian Zhao, Haoyang Xin, Mengli Xi
Summary: Through our research on wild species and cultivars, we have clarified the genetic relationships between different sections of tulips, as well as the differences and variations in the 5S rDNA sequences. Our findings provide clearer and more stable evidence for the classification of tulips.
SCIENTIA HORTICULTURAE
(2022)
Article
Genetics & Heredity
Jun He, Yong Zhao, Shuangshuang Zhang, Yanze He, Jiafu Jiang, Sumei Chen, Weimin Fang, Zhiyong Guan, Yuan Liao, Zhenxing Wang, Fadi Chen, Haibin Wang
Summary: This study used oligonucleotide fluorescence in situ hybridization (Oligo-FISH) to draw ideograms of rDNA sites in Chrysanthemum accessions and found polymorphisms and co-localization of rDNA sites. There was also a correlation between the number of 5S rDNA sites and chromosome number, and the geographical distribution of different ploidy Chrysanthemum accessions may have promoted karyotype evolution. The study identified the formation mechanism of rDNA variations and provided a reliable method for examining rDNA variations in Chrysanthemum and related species, contributing to understanding the evolutionary and phylogenetic relationships of the Chrysanthemum genus.
Article
Biochemistry & Molecular Biology
Jun He, Sisi Lin, Zhongyu Yu, Aiping Song, Zhiyong Guan, Weimin Fang, Sumei Chen, Fei Zhang, Jiafu Jiang, Fadi Chen, Haibin Wang
Summary: Using rDNA as a FISH probe, researchers found that 5S rDNA sites are mainly located in subterminal chromosome regions, while the number and intensity of 45S rDNA sites differ from previously studied Chrysanthemum resources. These findings offer insight into the chromosomal structural variations in Chrysanthemum and its related species.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Inkyu Park, Bokyung Choi, Hanna Weiss-Schneeweiss, Soonku So, Hyeon-Ho Myeong, Tae-Soo Jang
Summary: This study conducted comparative analyses on the karyotypes of three Iris species in Korea, with an emphasis on the number and localization of 5S and 35S rDNA loci using FISH. The chloroplast genomes of the same individuals were also sequenced and compared. The results provide important insights into the origin of the progenitor species and the genomes of Iris series Chinenses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Angeles Cuadrado, Marta Sixto, Rosa I. Figueroa, Isabel Bravo, Alfredo de Bustos
Summary: Through FISH analysis, we found differences in chromosomal organization and rDNA repeat unit numbers among different Gambierdiscus species. The location and organization of 5S and 45S rDNA in the nucleus also vary among species. These species can be distinguished by their species-specific FISH rDNA phenotypes.
EUROPEAN JOURNAL OF PHYCOLOGY
(2022)
Article
Ecology
Camille Bernery, Clara Marino, Celine Bellard
Summary: Understanding the factors that contribute to the success of exotic species is crucial in invasion biology. In this study, the ecological, morphological, and behavioral traits of 222 exotic freshwater fish were assessed to determine their role in establishment and spread. It was found that trait variability plays a significant role in the ability of species to establish and spread.
FUNCTIONAL ECOLOGY
(2023)
Article
Plant Sciences
Marlon Garcia Paitan, Maricielo Postillos-Flores, Luis Rojas Vasquez, Maria Siles Vallejos, Alberto Lopez Sotomayor
Summary: In this study, FISH mapping and flow cytometry were used to assess genetic differences between wild and cultivated populations of Physalis peruviana L. (goldenberry). The results showed genetic differences at the molecular cytogenetic level and in genome size between the two populations. These findings have important implications for future cytogenetic and genomic studies as well as breeding programs in goldenberry populations.
Article
Parasitology
Karin Tsuchida, Veronica Flores, Gustavo Viozzi, Carlos Rauque, Misako Urabe
Summary: A new freshwater fish parasitic trematode species, Derogenes lacustris, was described in Argentinean Patagonia, while another species, Thometrema patagonica, was found in the Neuquen River basin. Molecular analysis showed differences in their phylogenetic positions.
PARASITOLOGY RESEARCH
(2021)
Article
Parasitology
Suellen A. Zatti, Bruno L. Araujo, Edson A. Adriano, Antonio A. M. Maia
Summary: The study describes a new species of freshwater parasite called Ceratomyxa ranunculiformis n. sp. that infects the gall bladder of the Amazonian sciaenid Plagioscion squamosissimus. The new species was identified based on its host, myxospore morphology, ribosomal rDNA gene sequencing, parasite distribution, and phylogenetic analysis. Molecular and morphological analysis confirmed the classification of the new species within the coelozoic genus Ceratomyxa, clustering with other Amazonian freshwater ceratomyxids in a well-supported clade.
PARASITOLOGY INTERNATIONAL
(2023)
Article
Fisheries
Hui Zhang, Yu Sun, Shurun Zhu, Wangchao He, Luojing Zhou, Rurong Zhao, Kaikun Luo, Chenchen Tang, Chun Zhang, Shaojun Liu
Summary: The study revealed that strong signals of 5S rDNA from the RCC genome appeared in the pachytene and metaphase stages of meiosis I cells in 4nAT, indicating pairing of homologous chromosomes in 4nAT occurred between identical chromosomes. Additionally, all bivalent pairs possessed one monochiasmate and were neatly arranged in the equatorial plate during meiosis I in 4nAT, ensuring normal separation of homologous chromosomes.
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
(2022)
Article
Limnology
LIU Yu, LIU Pengfei, BI Yanhui, ZHOU Zhigang
Summary: The linkage of 5S rRNA gene to 18S-5.8S-25S rRNA gene was reported in some species in Ochrophyta. Dual-color fluorescence in situ hybridization technique revealed the localization and physical unlinkage of rRNA genes in Saccharina japonica, providing insights into the cytogenetics of this kelp species.
JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2021)
Article
Cell Biology
Linjiao Wang, Maoyin Sheng, Xueliang Ren
Summary: Physical mapping of 18S and 5S rDNA sites in eight Nicotiana species using double-probe FISH revealed differences in chromosomal distribution among different species. These findings contribute to understanding the genome evolution of the genus Nicotiana and have implications for genetic breeding and germplasm innovation in tobacco.
Article
Biochemistry & Molecular Biology
Thaissa Boldieri de Souza, Marcos Letaif Gaeta, Cesar Martins, Andre Luis Laforga Vanzela
MOLECULAR BIOLOGY REPORTS
(2020)
Article
Agriculture, Dairy & Animal Science
M. E. Herkenhoff, A. O. Ribeiro, J. M. Costa, A. C. Oliveira, M. A. D. Dias, R. V. Reis Neto, A. W. S. Hilsdorf, D. Pinhal
Article
Fisheries
Danillo Pinhal, Rodrigo R. Domingues, Christine C. Bruels, Bruno L. S. Ferrette, Otto B. F. Gadig, Mahmood S. Shivji, Cesar Martins
REVIEWS IN FISH BIOLOGY AND FISHERIES
(2020)
Article
Cell Biology
Luiz Augusto Bovolenta, Danillo Pinhal, Marcio Luis Acencio, Arthur Casulli de Oliveira, Simon Moxon, Cesar Martins, Ney Lemke
Review
Biochemistry & Molecular Biology
Cesar Martins, Maryam Jehangir
Summary: B chromosomes (Bs) in cichlid fishes have been extensively studied using classical cytogenetics and high-scale DNA, RNA, and epigenetics analysis. They are found only in females in some species and contain genes, relics of genes, transposable elements, and sequences transcribing for significant biological functions. The genic content of B chromosomes in cichlids is related to cell cycle and chromosome structure, influencing sex rates and potentially affecting transcription and epigenetic modifications of the entire genome.
Review
Biodiversity Conservation
Rodrigo Rodrigues Domingues, Ingrid Vasconcellos Bunholi, Danillo Pinhal, Agostinho Antunes, Fernando Fernandes Mendonca
Summary: The challenge of precisely identifying shark and ray species from the fin trade due to high demand for shark fin soup in Asia has led to overexploitation of elasmobranch populations worldwide. Many studies have reported the presence of threatened and protected elasmobranchs in the Asian trade market, showing the failure of current management and conservation policies. New approaches include developing standardized DNA-based methods, high throughput sequencing protocols, and increasing knowledge about population genetics data to combat illegal shark and ray fin trade.
CONSERVATION GENETICS RESOURCES
(2021)
Article
Biotechnology & Applied Microbiology
Jordana Inacio Nascimento-Oliveira, Bruno Evaristo Almeida Fantinatti, Ivan Rodrigo Wolf, Adauto Lima Cardoso, Erica Ramos, Nathalie Rieder, Rogerio de Oliveira, Cesar Martins
Summary: This study is the first to report the connection of B chromosomes and miRNAs in a vertebrate species. The B chromosome impacts the miRNAs expression in several tissues and these miRNAs target several mRNAs involved with important biological processes. The differentially expressed miRNA targets in samples carrying the B chromosome are related to nuclear matrix organization and response to stimulus.
Article
Genetics & Heredity
William B. Domingues, Tony L. R. Silveira, Leandro S. Nunes, Eduardo B. Blodorn, Augusto Schneider, Carine D. Corcine, Antonio S. Varela Junior, Izani B. Acosta, Mateus T. Kuetter, Gonzalo Greif, Carlos Robello, Danillo Pinhal, Luis F. Marins, Vinicius F. Campos
Summary: The overexpression of growth hormone in gh-transgenic zebrafish affects various aspects of their physiology, behavior, and reproductive performance. The dysregulation of miRNAs in sperm due to gh overexpression leads to a reduction in sperm quality and reproductive potential in these transgenic zebrafish.
FRONTIERS IN GENETICS
(2021)
Review
Fisheries
Syed Farhan Ahmad, Maryam Jehangir, Kornsorn Srikulnath, Cesar Martins
Summary: The rapid transformation of fish genomics and the availability of high-quality datasets provide important roadmaps for research, highlighting fish genomes as vital resources for genomic studies.
REVIEWS IN FISH BIOLOGY AND FISHERIES
(2022)
Article
Genetics & Heredity
Leandro Silva Nunes, William Borges Domingues, Frederico Schmitt Kremer, Danillo Pinhal, Vinicius Farias Campos
Summary: In this study, we identified 135 transcription factors driving the cellular state transition process during zebrafish cardiac regeneration using a systematic review and in silico analysis. We found that most of these regulators are activated in the first few days post-injury, indicating a prompt transition from a non-regenerative to a regenerative state.
Article
Genetics & Heredity
Matheus Azambuja, Viviane Nogaroto, Orlando Moreira-Filho, Cesar Martins, Michelle Orane Schemberger, Marcelo Ricardo Vicari
Summary: By analyzing the U snRNA genes in Apareiodon sp., this study identified nine snRNA multigene families and recognized functional/defective copies. In addition, in situ localization of snDNAs revealed their involvement in chromosomal location changes and dispersion. The findings suggest that defective copies trigger chromosome differentiation and diversification events in Parodontidae.
Article
Biochemistry & Molecular Biology
Bruno Rafael Ribeiro de Almeida, Renata Coelho Rodrigues Noronha, Adauto Lima Cardoso, Cesar Martins, Jonas Gama Martins, Rudi Emerson de Lima Procopio, Cleusa Yoshiko Nagamachi, Julio Cesar Pieczarka
Summary: This study investigates meiotic associations and failures in multiple species of Tityus scorpions, as well as the expression of genes in the recombination process. The results show the importance of gamma H2AX and holocentric chromosomes in maintaining the quadrivalent and normal meiotic cycle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Adauto Lima Cardoso, Natalia Bortholazzi Venturelli, Irene da Cruz, Fabio Malta de Sa Patroni, Diogo de Moraes, Rogerio Antonio de Oliveira, Ricardo Benavente, Cesar Martins
Summary: In this study, the meiotic behavior and transmission rates of the B chromosome in A. latifasciata were investigated. The B chromosome was found to have self-pairing behavior in the meiotic structure, indicating that isochromosome formation may be a step in the evolution of the B chromosome. Additionally, copies of cell cycle genes were identified in the B chromosome and were found to be transcribed in the encephalon, muscle, and gonads of the host, potentially providing beneficial effects and contributing to the maintenance of the B chromosome.
MOLECULAR GENETICS AND GENOMICS
(2022)
Article
Genetics & Heredity
Renata C. R. Noronha, Bruno R. R. Almeida, Monique C. S. Chagas, Flavia S. Tavares, Adauto L. Cardoso, Carlos E. M. C. Bastos, Natalia K. N. Silva, Alex G. C. M. Klautau, Fabia O. Luna, Fernanda L. N. Attademo, Danielle S. Lima, Luiz A. Sabioni, Maria I. C. Sampaio, Jairo Moura Oliveira, Luis Adriano Santos do Nascimento, Cesar Martins, Marcelo R. Vicari, Cleusa Y. Nagamachi, Julio C. Pieczarka
Summary: Significant efforts have been made to preserve manatees, with a recent study identifying a hybrid zone between Trichechus inunguis and Trichechus manatus manatus. Karyotype analysis revealed differences among manatee hybrids, suggesting they may represent different generations formed through crossing or F1 generations with karyotype variations resulting from different mechanisms.
Article
Biology
Jordana Inacio Nascimento Oliveira, Adauto Lima Cardoso, Ivan Rodrigo Wolf, Rogerio Antonio de Oliveira, Cesar Martins
Summary: This study characterized PIWI-interacting RNA (piRNA) clusters and pathways responsible for silencing the mobilization of transposable elements (TEs) in the gonads of the cichlid fish Astatotilapia latifasciata carrying the B chromosome. The study predicted and annotated piRNA clusters in the A. latifasciata genome for the first time and observed biased expression related to sex and the presence of the B chromosome. The study also identified three piRNA clusters in the B chromosome, with two of them exclusively expressed in gonads of samples with the B chromosome. The study suggests that the B chromosome has its own genomic guardians to prevent uncontrolled TE mobilization.