Article
Biochemistry & Molecular Biology
Shenglin Liu, Aja Noersgaard Buur Tengstedt, Magnus W. Jacobsen, Jose Martin Pujolar, Bjarni Jonsson, Javier Lobon-Cervia, Louis Bernatchez, Michael M. Hansen
Summary: This study analyzed genetic and methylation variation in European eels and found that methylation plays an important role in the life cycle of eels, potentially mediating interactions between local environments, development, and phenotypic variation for adaptation. The study also highlighted the significant role of methylation at Hox genes for adaptive processes.
Article
Biochemistry & Molecular Biology
Lucila Babio, Erin L. Damsteegt, P. Mark Lokman
Summary: Novel sequences of lr and lrp genes expressed in the ovary of the short-finned eel during early gonadal development were identified. The genes lr8 +, lr8-, and lrp13 were highly expressed in the ovary and may encode putative Vtg receptors in anguillid eels.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Fisheries
Ilaria Guarniero, Daniele Franchini, Alice Ferrari, Laura Gentile, Antonio Casalini, Pietro Emmanuele, Oliviero Mordenti
Summary: Due to its complex life cycle and multiple stress factors, the European eel has been declared Critically Endangered. Implementing mass production through artificial breeding protocols could be a possible solution to relieve pressure on natural stocks.
AQUACULTURE REPORTS
(2023)
Review
Fisheries
Bernd Pelster
Summary: Eels use their swimbladder for buoyancy control, which is achieved through the activity of gas gland cells and countercurrent concentration. Gas gland cells produce lactic acid and CO2, which acidify the blood and release oxygen from hemoglobin. The diffusion of oxygen and CO2 into the swimbladder is facilitated by the diffusion gradients created by elevated PO2 and PCO2. Infection of the swimbladder with the nematode Anguillicola crassus impairs its function as a buoyancy organ.
Article
Fisheries
C. M. F. Durif, M. Arts, F. Bertolini, A. Cresci, F. Daverat, E. Karlsbakk, J. Koprivnikar, E. Moland, E. M. Olsen, C. Parzanini, M. Power, M. Rohtla, A. B. Skiftesvik, E. Thorstad, L. A. Vollestad, H. Browman
Summary: This article discusses the life cycle strategies of anguillid eels and introduces methods for studying these strategies. The article points out that the current management framework for eels focuses mainly on freshwater environments, ignoring eels growing in saline waters. The authors argue that the ability of eels to shift habitats is crucial for the recovery of endangered species.
ICES JOURNAL OF MARINE SCIENCE
(2023)
Article
Multidisciplinary Sciences
Wayne M. Koster, Kim Aarestrup, Kim Birnie-Gauvin, Ben Church, David Dawson, Jarod Lyon, Justin O'Connor, David Righton, Denis Rose, Hakan Westerberg, Ivor Stuart
Summary: Anguillid eel populations have declined dramatically over the past 50 years in many regions, with numerous species now facing extinction. Research on the oceanic spawning migrations of the Australasian short-finned eel revealed that they mainly access deep waters off the Australian coast through two main routes: directly east via Bass Strait, or southeast around Tasmania, which is the shortest route.
SCIENTIFIC REPORTS
(2021)
Article
Biodiversity Conservation
Xiu Feng, Shenglin Liu, Michael M. Hansen
Summary: European and American eel are catadromous fish species that have experienced recent population declines. The demographic histories of both species show ancient declines and stable periods, with possible environmental factors including ocean current changes and geomagnetism reversal.
CONSERVATION GENETICS
(2022)
Article
Cell Biology
Lucila Babio, P. Mark Lokman, Erin L. Damsteegt, Ludovic Dutoit
Summary: Through comparing the transcriptomes at different stages of eel ovaries, this study found that specific genes encoding cell junction proteins may play a role in regulating Vtg uptake. The expression changes of key proteins supported the mechanical barrier hypothesis, and the endocytic pathway was found to be up-regulated during Vtg uptake. The study also provided new sequence data for A. australis.
Article
Multidisciplinary Sciences
Kasun A. Bandara, Elisa Benini, Sebastian N. Politis, Luis E. C. Conceicao, Andre Santos, Sune Riis Sorensen, Jonna Tomkiewicz, Olav Vadstein
Summary: The critical period between 20 to 24 days post hatch in European eels is associated with larvae-bacterial interactions and the larval immune status. During this period, the larval stress/repair mechanism is activated, and there is a shift towards a potentially detrimental larval bacterial community. However, larvae fed with a specific diet show higher expression of immune genes and better survival compared to other groups.
Article
Environmental Sciences
Helene Ronquist Knutsen, Sune Riis Sorensen, Peter Munk, Tora Bardal, Elin Kjorsvik
Summary: The study characterized the digestive system of European eel leptocephalus larvae, finding features such as an expandable pharynx/esophagus and a linear relationship between intestine length and larval size. The findings support the hypothesis that the larvae may feed on digestible gelatinous plankton and marine snow aggregates.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Ying-Ying Li, Jin-Xian Yang, Xi Chen, Qiang Chen, Tie-Ying Song, Jun-Qing Ge
Summary: This study investigated the molecular mechanisms and immune response in the skin mucus of Anguilla anguilla infected with Anguillid herpesvirus 1 (AngHV) at the protein levels. TMT-labelled proteomics with LC-MS/MS was used for quantitative identification of proteins. The results showed significant changes in protein expression, and many differentially expressed proteins were found to be enriched in various pathways, providing important insights into the pathogenesis of AngHV.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Jeremy Denis, Khalef Rabhi, Francois Le Loc'h, Frida Ben Rais Lasram, Kevin Boutin, Maria Kazour, Mamadou Diop, Marie-Christine Gruselle, Rachid Amara
Summary: This study characterizes and compares the feeding ecology of European eels in different salinity gradients in northern France estuaries. The research finds that eels mainly feed on crustaceans and fish, and their trophic level varies among different estuaries. The results are important for understanding the ecology and environmental adaptability of eels.
Article
Environmental Sciences
Concepcion Martinez-Gomez, Beatriz Fernandez, Elena Barcala, Victor Garcia-Aparicio, Esther Jumilla, Angel Gea-Pacheco, Victor Manuel Leon
Summary: This study provides an initial overview of the impact of organic chemical contaminants on the European eel and the potential sublethal effects of chemical pollution on pre-migrating eels in the Mar Menor coastal lagoon. The findings reveal that eels in this habitat are exposed to high levels of legacy organochlorine contaminants, banned pesticides, and emerging chemicals, and they show biomarker responses indicating genotoxic effects.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Fisheries
Sebastian N. Politis, Elisa Benini, Joanna J. Miest, Sofia Engrola, Sune R. Sorensen, Elisavet Syropoulou, Ian A. E. Butts, Jonna Tomkiewicz
Summary: In this study, prebiotics, probiotics, and synbiotics were administered to European eel larvae to stimulate gut-priming. The results showed that synbiotics increased mortality, while probiotics and synbiotics impaired growth. Larvae receiving prebiotics showed similar survival and growth to the control group. Water management strategies and rearing options need to be further explored to establish optimized culture conditions for future eel larval culture.
AQUACULTURE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Hiroyuki Inaba, Yuzo Iwata, Takashi Suzuki, Moemi Horiuchi, Ryohei Surugaya, Shigeho Ijiri, Ai Uchiyama, Ryoko Takano, Seiji Hara, Takashi Yazawa, Takeshi Kitano
Summary: In this study, the effects of the phytoestrogens genistein and daidzein on sex differentiation and sex-specific genes in Japanese eels were examined. Genistein was found to induce feminization more efficiently than daidzein, by altering the molecular pathways responsible for eel sex differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Rob C. Van Wijk, Astrid M. van der Sar, Elke H. J. Krekels, Theo Verboom, Herman P. Spaink, Ulrika S. H. Simonsson, Piet H. van der Graaf
CTS-CLINICAL AND TRANSLATIONAL SCIENCE
(2020)
Article
Multidisciplinary Sciences
Axel Meyer, Siegfried Schloissnig, Paolo Franchini, Kang Du, Joost M. Woltering, Iker Irisarri, Wai Yee Wong, Sergej Nowoshilow, Susanne Kneitz, Akane Kawaguchi, Andrej Fabrizius, Peiwen Xiong, Corentin Dechaud, Herman P. Spaink, Jean-Nicolas Volff, Oleg Simakov, Thorsten Burmester, Elly M. Tanaka, Manfred Schartl
Summary: Lungfishes, belonging to lobe-fined fish, possess a chromosome-quality genome with a vast size attributed to huge intergenic regions, introns with high repeat content, and active transposable elements. Phylogenomic analyses confirm lungfishes' key evolutionary position as the closest living relatives to tetrapods. Their preadaptations to living on land, increased rates of evolution, and duplication of genes associated with obligate air-breathing contribute to their tetrapod-like biology. These findings advance our understanding of the major transition during vertebrate evolution.
Article
Endocrinology & Metabolism
Junling He, Yi Ding, Natalia Nowik, Charel Jager, Muhamed N. H. Eeza, A. Alia, Hans J. Baelde, Herman P. Spaink
Summary: The study demonstrates that lepb regulates glucose homeostasis and adiposity in zebrafish, with lepb(-/-) mutant zebrafish showing features of T2DM. Additionally, lepb(-/-) adult zebrafish exhibit early signs of diabetic nephropathy, making them a promising model for researching the development and treatment of T2DM and its complications.
JOURNAL OF ENDOCRINOLOGY
(2021)
Article
Pathology
Rohola Hosseini, Gerda E. M. Lamers, Erik Bos, Pancras C. W. Hogendoorn, Abraham J. Koster, Annemarie H. Meijer, Herman P. Spaink, Marcel J. M. Schaaf
Summary: The study used zebrafish larvae infected with Mycobacterium marinum as an animal model for tuberculosis. The results showed that Myd88 deficiency led to more compact granuloma structures and a reduced number of leukocytes. Additionally, the majority of bacteria in the Myd88 mutant were located extracellularly, while most bacteria in the wild type were intracellular.
Article
Cell Biology
Wanbin Hu, Leonie van Steijn, Chen Li, Fons J. Verbeek, Lu Cao, Roeland M. H. Merks, Herman P. Spaink
Summary: In this study using zebrafish larval tail-wounding model, it was found that TLR2 and MyD88 play a role in regulating neutrophil and macrophage cell migration behavior, influencing the direction of neutrophil migration away from the wound and the speed of macrophage migration towards the wound edge. This indicates the involvement of TLR2 and MyD88 in responses to tail wounding by modulating leukocyte migration behavior and speed in vivo.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biology
Saloni Saxena, Herman P. Spaink, Gabriel Forn-Cuni
Summary: The review focuses on the rise in infections caused by nontuberculous mycobacteria (NTM) and their resistance to conventional antibiotics, proposing strategies to develop better therapies against them. It suggests accelerating the drug development pipeline by utilizing a combination of computational, laboratory and animal testing methods. The review also highlights the importance of understanding NTM virulence and developing new effective antimicrobial therapies.
Article
Toxicology
Bjorn E. Koch, Herman P. Spaink, Annemarie H. Meijer
Summary: Many facial bony features develop from preexisting collagen-rich cartilage structures through endochondral ossification. The proper development of these cartilage structures is crucial for facial morphological formation, which is sensitive to compounds that disturb histone acetylation patterns. A quantitative morphological assay using zebrafish larvae exposed to histone deacetylase inhibitors has been developed to measure the ceratohyal bone angle, providing a precise and sensitive proxy for overall facial cartilage development. This assay has been demonstrated to be applicable for investigating structure-activity relationships using valproic acid and related compounds.
TOXICOLOGY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Yi Ding, Marielle C. Haks, Gabriel Forn-Cuni, Junling He, Natalia Nowik, Amy C. Harms, Thomas Hankemeier, Muhamed N. H. Eeza, Jorg Matysik, A. Alia, Herman P. Spaink
Summary: This study investigates the effects of leptin deficiency in mice and zebrafish larvae through integration of metabolomics and transcriptomics, revealing similar metabolic alterations and dysregulated gene regulation related to proteolysis and arachidonic acid metabolism in both models.
CELL AND BIOSCIENCE
(2021)
Article
Cell Biology
Natalia Nowik, Tomasz K. Prajsnar, Anna Przyborowska, Krzysztof Rakus, Waldemar Sienkiewicz, Herman P. Spaink, Piotr Podlasz
Summary: Galanin, a conserved peptide found across different species, was traditionally thought to be related to neurotransmission but recent studies have shown its involvement in immune responses. Knockout of galanin in zebrafish increased susceptibility to Mycobacterium marinum and Staphylococcus aureus infections, while administration of a galanin analogue improved the fish's ability to control the infections. Transcriptional data revealed fewer gene regulations in response to mycobacterial infections in gal-/- mutants compared to gal+/+ counterparts, indicating a significant impact of galanin deficiency on immune-related pathways. The results suggest that galanin not only serves as a neurotransmitter, but also has implications in immune responses to bacterial infections, highlighting the complexity of the neuroendocrine system and its connection to immunity.
Article
Chemistry, Multidisciplinary
Doris A. van Bergeijk, Somayah S. Elsayed, Chao Du, Isabel Nunez Santiago, Anna M. Roseboom, Le Zhang, Victor J. Carrion, Herman P. Spaink, Gilles P. van Wezel
Summary: The biosynthetic gene clusters of Actinobacteria are poorly expressed in the laboratory. However, the stress hormone epinephrine has been found to promote their expression and influence the metabolism of Streptomyces. This response is mediated by the catechol moiety.
COMMUNICATIONS CHEMISTRY
(2022)
Article
Biology
Wanbin Hu, Herman P. Spaink
Summary: This review summarizes the functions of Toll-like receptor 2 (TLR2) in innate immunity and metabolism, and suggests future research directions.
Review
Medicine, Research & Experimental
Fleur M. Keij, Bjorn E. Koch, Fernando Lozano Vigario, Sinno H. P. Simons, Johan G. C. van Hasselt, H. Rob Taal, C. A. J. Knibbe, Herman P. Spaink, Irwin K. M. Reiss, Elke H. J. Krekels
Summary: Zebrafish larvae show promise as a preclinical model for neonatal sepsis due to their immune system similarities to humans. Research has demonstrated that zebrafish can be used to study infections causing neonatal sepsis, aiding in the exploration of new treatment approaches and biomarkers.
JOURNAL OF CLINICAL AND TRANSLATIONAL SCIENCE
(2021)
Article
Biochemical Research Methods
Niels M. Leijten, Petra Bakker, Herman P. Spaink, Jeroen den Hertog, Simone Lemeer
Summary: Thermal proteome profiling (TPP) enables unbiased detection of drug-target protein engagements in vivo. By using whole zebrafish embryo lysate, this study identified potential protein targets of napabucasin, including oxidoreductase PORA, which may affect Stat3 signaling.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Biology
M. Liem, T. Regensburg-Tuink, C. Henkel, H. Jansen, H. Spaink
Summary: Using only three ONT flow cells, thousands of organisms, including bacteriophages, were identified, with a large part at species level. Genomes could be assembled from environmental samples with Flye, resulting in >1 Mbp contigs in several cases and a near complete genome for a Thioglobus singularis species. k-mer analysis revealed that a significant portion of the data represented species not yet deposited in the database. This approach is suitable for scalable genomic investigations and provides a new platform for education in biodiversity.
BMC RESEARCH NOTES
(2021)
Article
Pharmacology & Pharmacy
Diyah Tri Utami, Sylvia Utami Tunjung Pratiwi, Herman P. Spaink, Tetiana Haniastuti, Triana Hertiani
Summary: This study investigated the efficacy of C-10 Massoia lactone in oral polymicrobial degradation, demonstrating its ability to degrade biofilms and destroy extracellular polymeric substances. Further research is needed to confirm these findings.
JOURNAL OF ADVANCED PHARMACEUTICAL TECHNOLOGY & RESEARCH
(2021)