Article
Cell Biology
Ana A. Jones, Elvira Diamantopoulou, Sarah Baxendale, Tanya T. Whitfield
Summary: Epithelial morphogenesis of the zebrafish inner ear depends on the production and localization of glycosaminoglycans, particularly hyaluronan and proteoglycans, which play critical roles in epithelial projection outgrowth and otolith development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Aurora K. Vikan, Michal Kostas, Ellen Margrethe Haugsten, Pal K. Selbo, Jorgen Wesche
Summary: The study evaluated the efficacy and selectivity of PCI of FGF2-saporin in cells overexpressing FGFR1, demonstrating that PCI enhances cytotoxicity of the drug. The cytotoxic effect due to internalization by HSPGs can be reduced through competitive inhibition.
Article
Ophthalmology
Tayler F. L. Wishart, Frank J. Lovicu
Summary: The study showed that all HSPGs are expressed in the lens, and they are differentially localized in distinct functional regions. Inhibition of HS-sulfation with Surfen blocked FGF-2-induced cell proliferation and fiber differentiation in vitro, suggesting the dependency of these cellular processes on HSPGs.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Biology
James M. Clegg, Thomas Pratt
Summary: The LACE assay is a powerful tool for investigating molecular interactions between heparan sulphate and diffusible ligands, detecting changes in binding following genetic or pharmacological interventions, and quantifying alterations in fluorescent signals in response to changes in HS sulphation.
Article
Cell Biology
Tayler F. L. Wishart, Frank J. Lovicu
Summary: Heparan sulphate proteoglycans (HSPGs) are composed of a core protein and sulphated HS-glycosaminoglycan (GAG) chains. These HS-GAG chains bind to and regulate the activity of HS-binding proteins, including growth factors, playing a crucial role in cellular processes during lens development. The different localisation of HSPG core proteins suggests their specialized roles in lens induction and morphogenesis.
Article
Virology
Sinead Carse, Dirk Lang, Arieh A. Katz, Georgia Schaefer
Summary: The cell-surface-expressed vimentin has been identified as a novel binding molecule modulating the infectious potential of HPV16-PsVs. It inhibits HPV16-PsVs internalisation transiently by affecting multiple steps during the virus' interaction with the host cell. Further refinement is needed before vimentin can be developed as an alternative prophylactic means against HPV infection.
Article
Biochemistry & Molecular Biology
Caixia Xian, Mingwei Zhu, Tianying Nong, Yiqiang Li, Xingmei Xie, Xia Li, Jiangui Li, Jingchun Li, Jianping Wu, Weizhe Shi, Ping Wei, Hongwen Xu, Ya-Ping Tang
Summary: Hereditary multiple exostoses (HME) is a rare skeletal disorder caused by mutations in ext1 and ext2 genes. A novel splice site mutation in ext2 was identified as a potential pathogenic mutation in HME.
GENETICS AND MOLECULAR BIOLOGY
(2021)
Article
Cell Biology
Alexander M. Lovely, Timothy J. Duerr, Qingchao Qiu, Santiago Galvan, S. Randal Voss, James R. Monaghan
Summary: The study shows that the Wnt signaling pathway plays a similar role in both limb development and limb regeneration in axolotl salamanders. This provides new insights into the interaction of Wnt signaling with other signaling pathways during salamander limb development and regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Physiology
Cassandra J. Field, Alexis M. Perez, Taryn Samet, Victoria Ricles, M. Kathryn Iovine, Linda J. Lowe-Krentz
Summary: Tmem184a plays a critical role in the proper formation of blood vessels in zebrafish embryos. It acts synergistically with Vegfr2b to modulate angiogenesis and affects endothelial cell proliferation and VE-cadherin expression.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
M. Fethullah Simsek, Ertugrul M. Ozbudak
Summary: The study presents a flattened 3-D tissue culture method for live imaging of zebrafish tail explants, allowing for a proportional slowdown of axis elongation and untangling the chemical input of signaling gradients from the mechanistic input of axial elongation. This method can provide new insights for future studies on vertebrate segmentation and potential pharmaceutical perturbations.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Environmental Sciences
Manar G. Helal, Ahmed G. Abd Elhameed
Summary: The study demonstrates that graviola leaves exhibit protective effects against acetic acid-induced ulcerative colitis in rats, reducing colonic damage and oxidative stress while modulating apoptosis and Wnt/Hh signaling pathways.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Developmental Biology
Benoit Haerlingen, Robert Opitz, Isabelle Vandernoot, Angelo Molinaro, Meghna Parakkal Shankar, Pierre Gillotay, Achim Trubiroha, Sabine Costagliola
Summary: Thyroid tissue is derived from ventral pharyngeal endoderm and defects in morphogenesis are a main cause of congenital thyroid diseases. Recent studies have revealed the importance of FGF and BMP signaling in thyroid specification, but the relationship between signaling cues and thyroid transcription factors is still unclear. By analyzing zebrafish embryos, researchers identified a population of Pax2a-expressing thyroid progenitor cells that show enhanced FGF signaling but lack Nkx2.4b expression and BMP signaling. Manipulation of FGF/BMP activities suggests that FGF signaling mainly regulates Pax2a expression, while BMP signaling regulates both Pax2a and Nkx2.4b expression. Similar expression dynamics were observed in mouse embryos, indicating that this refined model of thyroid cell specification is evolutionarily conserved in mammals.
Article
Biochemistry & Molecular Biology
Shannon Faris, Weihua Jin, James Gibson, Anqesha Murray, Nathan Smith, Peng He, Fuming Zhang, Robert Linhardt, Chunyu Wang
Summary: Tauopathies are neurodegenerative diseases characterized by abnormal tau deposits in the brain. In this study, the researchers developed an AlphaScreen assay to target the tau-glycan interface and screened hundreds of compounds. They discovered a small-molecule compound, A9, which effectively disrupted the tau-heparin interaction and showed promising potential as a lead compound for tauopathies.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jialiang Liu, Guangli Ren, Kangshuai Li, Zengli Liu, Yue Wang, Tianli Chen, Wentao Mu, Xiaoqing Yang, Xingyong Li, Anda Shi, Wei Zhao, Bowen Xu, Jianhua Chang, Sen Guo, Chang Pan, Tao Zhou, Zongli Zhang, Yunfei Xu
Summary: This study revealed that coexpression of Smad4 and MYO18A is a favorable prognostic indicator for iCCA and pCCA, suppressing CCA proliferation and metastasis by regulating beta-catenin phosphorylation and intracellular localization.
CELL DEATH AND DIFFERENTIATION
(2022)
Review
Biochemistry & Molecular Biology
Christina A. Daly, Eric T. Hall, Stacey K. Ogden
Summary: Cells communicate with their neighbors during development and tissue homeostasis to ensure coordinated responses to signals. One way this communication occurs is through specialized extensions called cytonemes. However, the molecular mechanisms of cytoneme initiation, growth, and signal delivery are still not fully understood.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Gaurav K. Varshney, Wuhong Pei, Matthew C. LaFave, Jennifer Idol, Lisha Xu, Viviana Gallardo, Blake Carrington, Kevin Bishop, MaryPat Jones, Mingyu Li, Ursula Harper, Sunny C. Huang, Anupam Prakash, Wenbiao Chen, Raman Sood, Johan Ledin, Shawn M. Burgess
Article
Biochemistry & Molecular Biology
Gaurav K. Varshney, Suiyuan Zhang, Wuhong Pei, Ashrifia Adomako-Ankomah, Jacob Fohtung, Katherine Schaffer, Blake Carrington, Anoo Maskeri, Claire Slevin, Tyra Wolfsberg, Johan Ledin, Raman Sood, Shawn M. Burgess
NUCLEIC ACIDS RESEARCH
(2016)
Editorial Material
Developmental Biology
Gareth Wyn Griffiths, Ferenc Mueller, Johan Ledin, E. Elizabeth Patton, Tor Gjoen, Viola Helene Lobert, Hanne Cecilie Winther-Larsen, Mary Mullins, Jean-Stephane Joly, Finn-Arne Weltzien, Charles McLean Press, Peter Alestrom
Article
Anatomy & Morphology
Beata Filipek-Gorniok, Katarina Holmborn, Tatjana Haitina, Judith Habicher, Marta Bastos Oliveira, Charlotte Hellgren, Inger Eriksson, Lena Kjellen, Johan Kreuger, Johan Ledin
DEVELOPMENTAL DYNAMICS
(2013)
Article
Biochemistry & Molecular Biology
Katarina Holmborn, Judith Habicher, Zsolt Kasza, Anna S. Eriksson, Beata Filipek-Gorniok, Sandeep Gopal, John R. Couchman, Per E. Ahlberg, Malgorzata Wiweger, Dorothe Spillmann, Johan Kreuger, Johan Ledin
JOURNAL OF BIOLOGICAL CHEMISTRY
(2012)
Article
Multidisciplinary Sciences
Anthony J. Hayes, Scott Reynolds, Mari A. Nowell, Lee B. Meakin, Judith Habicher, Johan Ledin, Andrew Bashford, Bruce Caterson, Chrissy L. Hammond
Article
Multidisciplinary Sciences
Beata Filipek-Gorniok, Pernilla Carlsson, Tatjana Haitina, Judith Habicher, Johan Ledin, Lena Kjellen
Article
Multidisciplinary Sciences
Judith Habicher, Tatjana Haitina, Inger Eriksson, Katarina Holmborn, Tabea Dierker, Per E. Ahlberg, Johan Ledin
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Cell Biology
Alexandra Abramsson, Sindhulakshmi Kurup, Marta Busse, Shuhei Yamada, Per Lindblom, Edith Schallmeiner, Denise Stenzel, Dominique Sauvaget, Johan Ledin, Maria Ringvall, Ulf Landegren, Lena Kjellen, Goran Bondjers, Jin-ping Li, Ulf Lindahl, Dorothe Spillmann, Christer Betsholtz, Holger Gerhardt
GENES & DEVELOPMENT
(2007)
Article
Biochemistry & Molecular Biology
Johan Ledin, Maria Ringvall, Maria Thuveson, Inger Eriksson, Maria Wilen, Marion Kusche-Gullberg, Erik Forsberg, Lena Kjellen
JOURNAL OF BIOLOGICAL CHEMISTRY
(2006)
Article
Developmental Biology
WHJ Norton, J Ledin, H Grandel, CJ Neumann
Article
Biochemistry & Molecular Biology
K Holmborn, J Ledin, E Smeds, I Eriksson, M Kusche-Gullberg, L Kjellén
JOURNAL OF BIOLOGICAL CHEMISTRY
(2004)
Article
Biochemistry & Molecular Biology
J Ledin, W Staatz, JP Li, M Götte, S Selleck, L Kjellén, D Spillmann
JOURNAL OF BIOLOGICAL CHEMISTRY
(2004)
Article
Cell Biology
GJ Jenniskens, M Ringvall, WJH Koopman, J Ledin, L Kjellén, PHGM Willems, E Forsberg, JH Veerkamp, TH van Kuppevelt
JOURNAL OF CELL SCIENCE
(2003)