Review
Cell Biology
Samyuktha Suresh, Solene Huard, Thierry Dubois
Summary: CARM1, originally identified as a coregulator of transcription, has been found to have novel roles in autophagy, metabolism, paraspeckles, and early development apart from its well-established involvement in transcriptional coactivation. It is also gaining attention as a promising therapeutic target and drug response biomarker in certain types of cancer.
TRENDS IN CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marie-Michelle Genois, Jean-Philippe Gagne, Takaaki Yasuhara, Jessica Jackson, Sneha Saxena, Marie-France Langelier, Ivan Ahel, Mark T. Bedford, John M. Pascal, Alessandro Vindigni, Guy G. Poirier, Lee Zou
Summary: Recent research has shown that CARM1 interacts with PARP1 at DNA replication forks, slowing fork speed and promoting the stress response mechanism of fork reversal.
Article
Endocrinology & Metabolism
Yi Zhong, Yilong Wang, Xiaoguang Li, Haojie Qin, Shu Yan, Caijun Rao, Di Fan, Duqiu Liu, Fei Deng, Yanli Miao, Ling Yang, Kai Huang
Summary: This study reveals the essential role of protein arginine methyltransferase 4 (PRMT4) in the browning process of white adipose tissue (WAT), as well as its association with obesity. Upregulation of PRMT4 expression promotes WAT browning and thermogenesis to protect against high-fat diet-induced obesity and metabolic disruptions.
Article
Cell Biology
Yunquan He, Jieyu Guo, Yueyang Yu, Jiayu Jin, Qingjun Jiang, Qinhan Li, Siyu Ma, Qi Pan, Jiayi Lin, Nan Jiang, Jinghua Ma, Yongbo Li, Yannan Hou, Xiuling Zhi, Lindi Jiang, Lefeng Qu, Elena Osto, Xinhong Wang, Xiangxiang Wei, Dan Meng
Summary: The role of BACH1 in the differentiation of VSMCs from hESCs has been investigated. The study found that BACH1 facilitates the expression of VSMC marker genes by binding to CARM1, leading to the in vitro differentiation of hESCs into VSMCs.
Article
Chemistry, Medicinal
Zhuqing Zhang, Zuhao Guo, Xiaowei Xu, Danyan Cao, Hong Yang, Yanlian Li, Qiongyu Shi, Zhiyan Du, Xiaobin Guo, Xin Wang, Danqi Chen, Ying Zhang, Lin Chen, Kaixin Zhou, Jian Li, Meiyu Geng, Xun Huang, Bing Xiong
Summary: This study identified a series of potent CARM1 inhibitors, among which compound 43 showed high potency and selectivity in vivo for solid tumors. The detailed immuno-oncology study confirmed the profound effects of compound 43 in tumor immunity, paving the way for future studies in solid tumor treatment and cancer immunotherapy.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Futaba Inoue, Kenbun Sone, Yusuke Toyohara, Saki Tanimoto, Yu Takahashi, Misako Kusakabe, Asako Kukita, Harunori Honjoh, Akira Nishijima, Ayumi Taguchi, Yuichiro Miyamoto, Michihiro Tanikawa, Takayuki Iriyama, Mayuyo-mori Uchino, Tetsushi Tsuruga, Osamu Wada-Hiraike, Katsutoshi Oda, Yutaka Osuga
Summary: This study investigated the role of CARM1 and the therapeutic effect of TP-064 in endometrial cancer. The results showed that CARM1 was significantly overexpressed in endometrial cancer tissues, and knockdown of CARM1 inhibited cell growth and induced apoptosis. TP-064 also inhibited endometrial cancer cell growth and colony formation. Therefore, CARM1 may be a potential therapeutic target for endometrial cancer.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Engineering, Biomedical
Lauren A. Boller, Stefanie M. Shiels, David C. Florian, Sun H. Peck, Jonathan G. Schoenecker, Craig Duvall, Joseph C. Wenke, Scott A. Guelcher
Summary: This study explores the effects of nHA dispersed in polymeric scaffolds on bone regeneration, finding that nHA has a dose-dependent impact on osteogenesis and promotes new bone formation in a site-specific manner. The findings provide new insights into designing synthetic bone grafts for complex fracture cases.
ACTA BIOMATERIALIA
(2021)
Article
Cell Biology
Tian Xia, Ming Liu, Quan Zhao, Jian Ouyang, Bing Chen, Peipei Xu
Summary: PRMT5 is upregulated in multiple myeloma, and its inhibition enhances cell pyroptosis, while the expression of CASP1 negatively correlated with PRMT5 can be restored by suppressing PRMT5, promoting cell pyroptosis in MM.
CELL DEATH & DISEASE
(2021)
Review
Biochemistry & Molecular Biology
Wenke Jin, Jin Zhang, Xiya Chen, Siwen Yin, Haiyang Yu, Feng Gao, Dahong Yao
Summary: CARM1 is a type I protein arginine methyltransferase that catalyzes arginine methylation of histone and non-histone substrates, and is closely related to cancer occurrence and progression. This review summarizes the molecular structure of CARM1, its regulatory pathways, and discusses its oncogenic functions in tumor development. Furthermore, representative CARM1 inhibitors are demonstrated, focusing on their design strategies and potential therapeutic applications. These findings shed light on the understanding of CARM1 mechanisms and provide insights for the development of targeted cancer therapy.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2023)
Article
Biochemistry & Molecular Biology
Angela Fernandez-Iglesias, Rocio Fuente, Helena Gil-Pena, Laura Alonso-Duran, Fernando Santos, Jose Manuel Lopez
Summary: The study investigated the formation of the epiphyseal bone plate using various microscopy techniques and found that the bone tissue is generated through a process different from the usual endochondral ossification. Osteoblasts deposit bone matrix on various surfaces, leading to the establishment of a core of bone sheet and subsequent thickening and reshaping. The processes occur in close relationship with cartilage, providing insight into the complex cellular events underlying bone formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Xiaoxi Yang, Chuiguo Sun, Xiangyu Meng, Guanghui Chen, Tianqi Fan, Chi Zhang, Zhongqiang Chen
Summary: This study revealed the differential gene expression profile associated with LGR and Wnt signaling pathway in thoracic ligamentum flavum cells (TLFCs) during different stages of ossification through RNA sequencing and gene ontology enrichment analysis. The findings suggest that LGR5 enhances osteogenesis by activating the Wnt signaling pathway in thoracic ossification of the ligamentum flavum (TOLF).
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Cell Biology
Marta Marchini, Mitchell R. Ashkin, Melina Bellini, Margaret Man-Ger Sun, Matthew Lloyd Workentine, Hamza Malik Okuyan, Roman Krawetz, Frank Beier, Campbell Rolian
Summary: The genetic and developmental mechanisms of limb formation are well understood, but how chondrocyte physiology modulates these mechanisms to produce differences in limb bone length is unclear. Through RNA sequencing and cell culture experiments, it was found that NKA plays a key role in chondrocyte differentiation and regulation of bone length, with implications for limb length diversity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Medicine, Research & Experimental
Jiaoyang Ning, Liu Chen, Gang Xiao, Yu Zeng, Wen Shi, Guilong Tanzhu, Rongrong Zhou
Summary: PRMTs are involved in the prognosis and metastasis of tumors, and their regulation of molecular mechanisms affects tumor metastasis. Inhibiting arginine methylation can reduce tumor metastasis, and this field holds promise for clinical application.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Cardiac & Cardiovascular Systems
Yiheng Zhang, Robin A. F. Verwilligen, Miriam de Boer, Timothy J. P. Sijsenaar, Miranda Van Eck, Menno Hoekstra
Summary: The PRMT4 inhibitor TP-064 has a dose-dependent impact on inflammatory and metabolic processes, but does not change atherosclerosis susceptibility in male apolipoprotein E knockout mice.
Article
Biochemistry & Molecular Biology
Yilong Wang, Shu Yan, Xuemei Liu, Fei Deng, Pengchao Wang, Liuye Yang, Lizhi Hu, Kai Huang, Jiangui He
Summary: PRMT4 promotes ferroptosis in DOX-induced cardiomyocyte ferroptosis by interacting with Nrf2 and inhibiting its nuclear translocation, which subsequently suppresses the transcription of GPX4. Activation of Nrf2 or administration of Fer-1 abolishes the detrimental role of PRMT4 in DOX-induced cardiomyocyte ferroptosis.
CELL DEATH AND DIFFERENTIATION
(2022)
Review
Biochemistry & Molecular Biology
Naoyuki Taniguchi, Yuki Ohkawa, Kento Maeda, Yoichiro Harada, Masamichi Nagae, Yasuhiko Kizuka, Hideyuki Ihara, Yoshitaka Ikeda
Summary: Cancer-related changes in glycans on cell surface proteins are regulated by glycosyltransferases and related genes, which play key roles in cancer metastasis and invasion. The significance of EMT and N-glycan branching glycosyltransferases needs to be reconsidered for potential therapeutic strategies. Specific glycosyltransferases involved in EMT or MET pathways may be difficult to define due to their roles in intermediate states.
MOLECULAR ASPECTS OF MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Yuki Ohkawa, Yoichiro Harada, Naoyuki Taniguchi
Summary: Glycosylation is a common posttranslational modification of proteins, regulated by various glycosyltransferases and glycosidases. The core fucose structure on N-glycans may have protective functions against emphysema and COPD. Glycomimetics using glycan analogs could be a promising therapeutic strategy for various medical conditions.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2021)
Article
Biochemistry & Molecular Biology
Naoyuki Taniguchi, Masatsune Murata
Summary: This special issue highlights the importance of glycation to the glycoscience community while celebrating the 30th anniversary of JMARS and honoring Professor Vincent Monnier for his contributions to the field.
GLYCOCONJUGATE JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Yoichiro Harada, Yuki Ohkawa, Kento Maeda, Naoyuki Taniguchi
Summary: The endoplasmic reticulum (ER) contains multiple quality control systems (QCS) that shape the glycoproteome of eukaryotic cells. One of these systems, glycan QCS, plays a crucial role in ensuring proper glycoprotein folding while also potentially increasing the risk of overall hypoglycosylation, leading to protein misfolding and cellular stress response activation.
Article
Biochemistry & Molecular Biology
Yuki Ohkawa, Yasuhiko Kizuka, Misaki Takata, Miyako Nakano, Emi Ito, Sushil K. Mishra, Haruna Akatsuka, Yoichiro Harada, Naoyuki Taniguchi
Summary: The study uncovered that the action of GnT-III on proteins is closely related to their tertiary structure, which can accommodate a bulky flipped-back conformation of modified glycans generated by bisecting GlcNAc.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Biochemistry & Molecular Biology
Naoyuki Taniguchi
MOLECULAR ASPECTS OF MEDICINE
(2021)
Editorial Material
Biochemistry & Molecular Biology
Naoyuki Taniguchi, Kento Maeda, Masato Kitano
Summary: This commentary discusses a highly cited paper by Yasuhisa Kono which focused on the mechanism of hydroxylamine autooxidation in the assay of superoxide dismutase activity. Kono's work contributed to the development and progress in enzyme assay systems.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Review
Biochemistry & Molecular Biology
Motoko Takahashi, Yoshihiro Hasegawa, Kento Maeda, Masato Kitano, Naoyuki Taniguchi
Summary: Glycosylation of cell surface receptors plays a crucial role in regulating signal transduction. This review focuses on the role of glycosyltransferases involved in N-glycan modification, specifically the effects of branching and elongation on signaling by cell surface receptors. The review also discusses the involvement of these enzymes in EMT/MET programs related to differentiation, cancer development, progression, and therapy resistance.
GLYCOCONJUGATE JOURNAL
(2022)
Biographical-Item
Biochemistry & Molecular Biology
Naoyuki Taniguchi, Ernesto Carafoli
Summary: This article expresses gratitude and admiration for Dr. William Lennarz for his contributions and accomplishments in the field of life science during his tenure as Editor-in-Chief of Biochemical and Biophysical Research Communications.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Kiyotaka Kondo, Yoichiro Harada, Miyako Nakano, Takehiro Suzuki, Tomoko Fukushige, Ken Hanzawa, Hirokazu Yagi, Koichi Takagi, Keiko Mizuno, Yasuhide Miyamoto, Naoyuki Taniguchi, Koichi Kato, Takuro Kanekura, Naoshi Dohmae, Kentaro Machida, Ikuro Maruyama, Hiromasa Inoue
Summary: Protein N-glycosylation in lung cancer sEVs may potentially reflect the histology of lung cancers.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yuriko Tachida, Saori Miura, Yui Muto, Hiroyuki Takuwa, Naruhiko Sahara, Akihiro Shindo, Yukio Matsuba, Takashi Saito, Naoyuki Taniguchi, Yasushi Kawaguchi, Hidekazu Tomimoto, Takaomi Saido, Shinobu Kitazume
Summary: The deposition of amyloid P in blood vessels of the brain is commonly observed in Alzheimer's disease patients. However, it is difficult to examine the contribution of cerebral amyloid angiopathy (CAA) to the pathogenesis of Alzheimer's disease in mouse models. Based on biochemical analyses, the researchers hypothesized that endothelial APP expression would be lower in rodents and subsequently generated mice that express human WT APP in endothelial cells. The mice showed increased levels of serum A beta and soluble APP, indicating the crucial role of endothelial APP in blood A beta levels. Crossing these mice with a specific APP knock-in mouse model led to an expanded CAA pathology.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Dermatology
Kota Tachibana, Yuki Ohkawa, Noriko Kanto, Kento Maeda, Shuichi Ohe, Taiki Isei, Yoichiro Harada, Naoyuki Taniguchi
Summary: Glycan expression plays an important role in melanoma cells, and the ectopic expression of keratan sulfate (KS) can enhance the invasiveness and adhesion capabilities of melanoma cells, as well as stimulate the phosphorylation of paxillin, contributing to the development of malignant phenotypes.
JOURNAL OF DERMATOLOGY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Naoyuki Taniguchi
JOURNAL OF BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Masato Kitano, Yasuhiko Kizuka, Tomoaki Sobajima, Miyako Nakano, Kazuki Nakajima, Ryo Misaki, Saki Itoyama, Yoichiro Harada, Akihiro Harada, Eiji Miyoshi, Naoyuki Taniguchi
Summary: Glycosylation is a common posttranslational modification of proteins that is tightly regulated by the subcellular location and transportation of glycosyltransferases. Knockdown of Rab11 results in enhanced sialylation of N-glycans, specifically through the altered localization of the alpha 2,3-sialyltransferase ST3GAL4. This indicates a novel mechanism of glycosyltransferase regulation by Rab11 in post-Golgi compartments.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Yoichiro Harada, Kazuki Nakajima, Shengtao Li, Tadashi Suzuki, Naoyuki Taniguchi
Summary: This article introduces a non-radiolabeling protocol for isolating, determining the structure, and quantifying N-glycan precursors, providing insights into the biosynthesis and degradation processes.
