Article
Virology
Genevieve F. Oliver, Liam M. Ashander, Abby C. Dawson, Yuefang Ma, Jillian M. Carr, Keryn A. Williams, Justine R. Smith
Summary: Retinopathy is a recognized complication of dengue, affecting hospitalized patients up to 10%. This study aimed to investigate the susceptibility of Muller cells to dengue virus (DENV) infection and their antiviral, inflammatory, and immunomodulatory responses in vitro. It was found that Muller cells can be infected by DENV and mount an immune response, but the response varies across different cell isolates and DENV strains. This research provides insights into the role of retinal Muller glial cells in dengue retinopathy.
Article
Neurosciences
Wei Wei, Piaopiao Hu, Mengqi Qin, Guiping Chen, Feifei Wang, Shengrui Yao, Ming Jin, Zhi Xie, Xu Zhang
Summary: SIRT4 is highly expressed in retinal Muller glial cells and is relevant to the expression of GS. SIRT4 does not appear to be essential in retinal development, but resveratrol, as an activator of SIRT4, can upregulate GS protein expression and protect the retina.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Genetics & Heredity
L. Francisco Sanhueza Salas, Alfredo Garcia-Venzor, Natalia Beltramone, Claudia Capurro, Debra Toiber, Dafne Magali Silberman
Summary: Retinal Muller glial cells show metabolic changes early in retinal disease and have the potential to dedifferentiate into neural stem cells under certain conditions. The involvement of glucose, oxidative stress, and epigenetic factors in this reprogramming process remains to be fully understood. Studying the gene expression profile in diabetic conditions may provide further insights into the response of Muller cells to metabolic impairment.
FRONTIERS IN GENETICS
(2021)
Article
Ophthalmology
Annette Zwanzig, Jie Meng, Heidi Mueller, Susanne Buerger, Manuela Schmidt, Maik Pankonin, Peter Wiedemann, Jan Darius Unterlauft, Wolfram Eichler
Summary: Secreted neuroprotective factors produced by Muller cells can promote RGC survival, and R28 cells are more resistant to apoptosis when co-cultured with Muller cells. Under hypoxia, IL-6 and VEGF are upregulated in Muller cells, and these factors can also alter the expression of Bcl-2 family members, which regulate apoptosis.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Cell Biology
Remi Karadayi, Julie Mazzocco, Laurent Leclere, Benedicte Buteau, Stephane Gregoire, Christine Belloir, Mounzer Koudsi, Pauline Bessard, Jean-Baptiste Bizeau, Elisabeth Dubus, Claire Fenech, Loic Briand, Lionel Bretillon, Alain M. Bron, Xavier Fioramonti, Niyazi Acar
Summary: Plasmalogen deficiency leads to reduced expression of Cx43 in the retina, which is crucial for important functions of Muller cells such as GJIC and cell migration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Sarah Palko, Nicholas J. Saba, Elias Mullane, Benjamin D. Nicholas, Yosuke Nagasaka, Jayakrishna Ambati, Bradley D. Gelfand, Akihito Ishigami, Paola Bargagna-Mohan, Royce Mohan
Summary: Muller glia (MG) have been found to play a crucial role in reactive gliosis and are associated with retinal degenerative diseases. This study shows that MG citrullination is broadly conserved in various models and human tissues. The enzyme PAD4 is responsible for the citrullination, and endfeet are identified as a location where citrullination initiates and sustains in retinal degeneration.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Ashley C. Kramer, Katherine Gurdziel, Ryan Thummel
Summary: The study revealed that adult zebrafish have the ability to completely regenerate their retinas through the re-entry of Muller glia into the cell cycle and differentiation into new photoreceptors after intense light exposure. Additionally, the research identified two peaks of MG gliosis, a distinct transcriptional shift between 5- and 10-days post lesion, and different patterns of transcriptional recovery of photoreceptor opsins at 28 days post lesion.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Raquel Lourenco, Ana S. Brandao, Jorge Borbinha, Rita Gorgulho, Antonio Jacinto
Summary: This study demonstrates that Yap plays a crucial role in regulating Muller glia response to injury, promoting cell cycle reentry and progenitor cell formation, leading to the differentiation of new photoreceptors in zebrafish retina regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Ophthalmology
Weijie Jiang, Siqi He, Logen Liu, Xia Meng, Jing Lu, Juan Li, Tuo Chen, Ying Xu, Qiguo Xiao, Ling Qi, Jia Zhang
Summary: This review summarizes the roles of miRNAs in retinal Muller glial cell function, emphasizing the potential application of miRNAs in retinal repair and regeneration.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2023)
Review
Cell Biology
Elisabeth C. Kugler, John Greenwood, Ryan B. MacDonald
Summary: The neurovascular unit is a complex multi-cellular structure consisting of various cell types such as endothelial cells, neurons, glia, smooth muscle cells, and pericytes. While neurovascular coupling is a key function of the NVU, the unit's functionality extends beyond this coupling to include signaling, metabolism, and homeostasis across different components. Glial cells have gained increased attention within the NVU and play central roles in its functionality and disease pathogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Isabella Palazzo, Levi J. Todd, Thanh V. Hoang, Thomas A. Reh, Seth Blackshaw, Andy J. Fischer
Summary: Muller glia (MG) in mammalian retinas are incapable of regenerating neurons after damage, whereas the MG in lower vertebrates regenerate functional neurons. Identification of cell signaling pathways and gene regulatory networks that regulate MG-mediated regeneration is key to harnessing the regenerative potential of MG. In this study, NFkB signaling was found to play a crucial role in regulating immune cell accumulation and suppressing the neurogenic potential of MG after damage. Inhibition of NFkB enhanced the reprogramming of MG into neuron-like cells. Additionally, TGF beta 2 signaling and suppression of NFI and Id transcription factors were coordinated with NFkB signaling to regulate MG-mediated regeneration.
Review
Cell Biology
Tian-En Si, Zhixiao Li, Jingjing Zhang, Songxue Su, Yupeng Liu, Shiyue Chen, Guang-Hua Peng, Jing Cao, Weidong Zang
Summary: Retinal degenerative diseases, which cause neuronal death and vision loss, can be treated by reprogramming Muller glia into stem or progenitor cells to regenerate the retina. Epigenetic mechanisms, such as DNA methylation and histone modification, play a crucial role in the reprogramming process. Understanding these mechanisms is important for developing Muller glial reprogramming therapy for retinal degenerative diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Neurosciences
Xiaohuan Zhao, Mengqiao Xu, Zhenzhen Zhao, Yimin Wang, Yang Liu, Ting Zhang, Xiaoling Wan, Mei Jiang, Xueting Luo, Yao Shen, Lei Chen, Minwen Zhou, Feng Wang, Xiaodong Sun
Summary: This study found that the probiotic Bifidobacterium can promote the survival of retinal ganglion cells and optic nerve regeneration. This effect is achieved by inhibiting microglia activation and promoting Muller cell activation, leading to a reduction in the risk of optic nerve injury.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Neurosciences
Whitney A. Thiel, Zachary Blume, Diana M. Mitchell
Summary: This study found that microglia are primarily responsible for the clearance of dying cells in the developing zebrafish retina, while Muller glia also have a limited role in this process. In the absence of microglia, Muller glia significantly increase their uptake of apoptotic cells, with several of these cells localized with the early phagosome/endosome marker Rab5. Additionally, lysosomal staining also increases in Muller glia in the absence of microglia. These findings highlight the phagocytic capacity and compensatory functions of Muller glia, and emphasize the need to consider the effects of microglial deficiency or depletion on other glial cell types.
Article
Cell Biology
Kaida Song, Zihao Lin, Lining Cao, Bowen Lu, Yuxi Chen, Shuqiang Zhang, Jianfeng Lu, Hui Xu
Summary: The zebrafish Sox11 homolog, Sox11b, regulates the migration and fate determination of Muller glia-derived progenitors (MGPCs) during retina regeneration. Sox11b knockdown alters nuclear morphology and radial migration of MGPCs, leading to changes in their distribution within the retina. Additionally, Sox11b knockdown affects photoreceptor regeneration and the numbers of newborn amacrines and retinal ganglion cells. Notch signaling is found to function downstream of Sox11b in this process. These findings highlight the key roles of Sox11b in MGPC migration and fate determination during retina regeneration in zebrafish and have implications for future retinal repair research in mammals.
NEURAL REGENERATION RESEARCH
(2023)
Article
Developmental Biology
Cassidy S. Bernstein, Mitchell T. Anderson, Chintan Gohel, Kayleigh Slater, Jeffrey M. Gross, Seema Agarwala
DEVELOPMENTAL BIOLOGY
(2018)
Article
Genetics & Heredity
Archana D. Siddam, Carole Gautier-Courteille, Linette Perez-Campos, Deepti Anand, Atul Kakrana, Christine A. Dang, Vincent Legagneux, Agnes Mereau, Justine Viet, Jeffrey M. Gross, Luc Paillard, Salil A. Lachke
Article
Anatomy & Morphology
Natalie Gath, Jeffrey M. Gross
DEVELOPMENTAL DYNAMICS
(2019)
Article
Developmental Biology
Katie L. Sinagoga, Alessandra M. Larimer-Picciani, Stephanie M. George, Samantha A. Spencer, James A. Lister, Jeffrey M. Gross
Review
Developmental Biology
Kristen M. Koenig, Jeffrey M. Gross
Review
Genetics & Heredity
Reza Raeisossadati, Merari F. R. Ferrari, Alexandre Hiroaki Kihara, Issam AlDiri, Jeffrey M. Gross
Summary: This review highlights the importance of epigenetic regulation in the developing vertebrate retina, emphasizing its role in cell differentiation and disease development. It also suggests future studies should focus on investigating these mechanisms to develop novel treatment strategies for various retinal disorders.
EPIGENETICS & CHROMATIN
(2021)
Article
Multidisciplinary Sciences
Lyndsay L. Leach, Nicholas J. Hanovice, Stephanie M. George, Ana E. Gabriel, Jeffrey M. Gross
Summary: Research has revealed that zebrafish RPE has strong intrinsic regenerative potential, with elements of immune response identified as critical mediators in the regeneration process. Macrophage/microglia cells are responsive to RPE damage and essential for the timely progression of regenerative response.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Ophthalmology
Stephanie M. George, Fangfang Lu, Mishal Rao, Lyndsay L. Leach, Jeffrey M. Gross
Summary: Diseases causing retinal pigment epithelium degeneration, such as age-related macular degeneration, are a leading cause of blindness worldwide. Current therapies for RPE regeneration are ineffective, highlighting the need for further research into the regenerative mechanisms of RPE.
PROGRESS IN RETINAL AND EYE RESEARCH
(2021)
Article
Developmental Biology
Si Chen, Kira L. Lathrop, Takaaki Kuwajima, Jeffrey M. Gross
Summary: The study identifies the essential role of JAK/STAT pathway activity in the survival of retinal ganglion cells (RGCs) after optic nerve injury, showing that immune responses directly contribute to RGC death. Blocking neuroinflammation or depleting macrophages/microglia rescues RGC survival post-injury.
Article
Genetics & Heredity
Rody Kingston, Dwarkesh Amin, Sneha Misra, Jeffrey M. Gross, Takaaki Kuwajima
Summary: The study demonstrates the neurodegenerative mechanisms based on the early susceptibility of peripheral ventrotemporal (VT) retinal ganglion cells (RGCs) after optic nerve injury, with serotonin transporter (SERT) identified as a key factor mediating VT RGC vulnerability. Loss of SERT affects VT RGC protection and axon regeneration, with GPNMB playing a role in the differential vulnerability of RGCs in different retinal regions. These findings provide insights into selective RGC vulnerability and potential neuroprotective strategies.
Article
Genetics & Heredity
Fangfang R. Lu, Lyndsay P. Leach, Jeffrey R. Gross, Gregory P. Copenhaver, David R. Hyde
Summary: This study reveals the important role of the mTOR signaling pathway in retinal pigment epithelium (RPE) cell regeneration and identifies a link between mTOR activity and immune responses. These findings are significant for developing therapies aimed at stimulating RPE cell regeneration in the eye.
Article
Anatomy & Morphology
Sarah E. E. Seese, Sanaa Muheisen, Natalie Gath, Jeffrey M. M. Gross, Elena V. V. Semina
Summary: This study identified heat shock proteins as interacting partners of MAB21L2/mab21l2 and suggests a role for this interaction in vertebrate eye development.
DEVELOPMENTAL DYNAMICS
(2023)
Article
Neurosciences
Jiwoon Lee, Bum-Kyu Lee, Jeffrey M. Gross
Summary: The zebrafish retina has strong regenerative potential, and Muller glia play a crucial role in this process. The transcriptional regulation by members of the Bromodomain (Brd) family is important for Muller glia-dependent retinal regeneration.
Article
Medicine, Research & Experimental
Jennifer J. Johnston, Chanjae Lee, Ingrid M. Wentzensen, Melissa A. Parisi, Molly M. Crenshaw, Julie C. Sapp, Jeffrey M. Gross, John B. Wallingford, Leslie G. Biesecker
COLD SPRING HARBOR MOLECULAR CASE STUDIES
(2017)
Correction
Anatomy & Morphology
Nicholas J. Hanovice, Emily McMains, Jeffrey M. Gross
DEVELOPMENTAL DYNAMICS
(2017)