Article
Medicine, Research & Experimental
Kazuto Yoshimi, Yuko Yamauchi, Takao Tanaka, Toshio Shimada, Moritoshi Sato, Tomoji Mashimo
Summary: PA-Cre knock-in mice were established to spatially regulate Cre recombinase activity with blue light exposure, offering a useful resource for elucidating gene function in vivo spatiotemporally.
LABORATORY INVESTIGATION
(2021)
Article
Cell Biology
Lars Schlotawa, Ana Lopez, Gentzane Sanchez-Elexpuru, Sylwia D. Tyrkalska, David C. Rubinsztein, Angeleen Fleming
Summary: Much of our understanding of the regulation of macroautophagy/autophagy comes from in vitro studies, and there is still a lack of knowledge about its regulation in different tissues in vivo. Upregulation of autophagy is considered a promising therapeutic strategy, so it is important to understand its function in different tissues through in vivo analysis. To achieve this, an inducible expression system has been developed in zebrafish to up- or downregulate autophagy, allowing the investigation of how cell- or tissue-specific changes in autophagic flux affect processes such as aging, inflammation, and neurodegeneration.
Article
Ophthalmology
Zongbo Wei, Caili Hao, Jian-Kang Chen, Lin Gan, Xingjun Fan
Summary: In this study, a lens-specific inducible Cre mouse model was established for gene targeting research in a specific spatiotemporal manner. The results showed that Cre recombinase leaked mainly in the lens cortex and nucleus, while no leakage was observed in the lens epithelium. Furthermore, administration of tamoxifen effectively suppressed target gene expression in both lens epithelium and cortex. The findings of this study are of great significance for the research of lens aging, posterior capsule opacification, and other areas requiring precision gene targeting.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Takahito Maki, Masahito Sawahata, Ichiro Akutsu, Shohei Amaike, Genki Hiramatsu, Daisuke Uta, Naotaka Izuo, Takahiko Shimizu, Kazuhiro Irie, Toshiaki Kume
Summary: This study evaluated phenotypic changes in AD mice models, revealing the crucial role of toxic Aβ conformers in AD pathology, leading to dysregulation of HIF proteins, affecting cognitive function and neuronal changes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Hematology
Latha Muniappan, Michihiro Okuyama, Aida Javidan, Devi Thiagarajan, Weihua Jiang, Jessica J. Moorleghen, Lihua Yang, Anju Balakrishnan, Deborah A. Howatt, Haruhito A. Uchida, Takaomi C. Saido, Venkateswaran Subramanian
Summary: The study revealed that calpain-2 deficiency can prevent Ang II-induced cytoskeletal structural protein fragmentation and AAA development, as well as stabilize and suppress rupture of established AAAs in mice. This suggests that targeting calpain-2 may hold therapeutic potential for treating AAA.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Hematology
Allison M. Dubner, Sizhao Lu, Austin J. Jolly, Tysen Noble, Tyler Hinthorn, Raphael A. Nemenoff, Karen S. Moulton, Mark W. Majesky, Mary C. M. Weiser-Evans
Summary: Tamoxifen treatment leads to decreased serum cholesterol, reduced plaque formation, and increased hepatic lipid accumulation. These alterations could significantly impact the results of atherosclerosis research.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Immunology
Tiffany Carr, Stephanie McGregor, Sheila Dias, Mihalis Verykokakis, Michelle M. Le Beau, Hai-Hui Xue, Mikael Sigvardsson, Elizabeth T. Bartom, Barbara L. Kee
Summary: T lymphocyte acute lymphoblastic leukemia (T-ALL) is a heterogeneous disease affecting T cells at multiple stages of their development. The timing of mutation in the transcription factor LEF1 impacts its function as a tumor suppressor or oncogene in T-ALL. Leukemic cells become addicted to the presence of LEF1, and deletion prior to transformation accelerates leukemogenesis and alters gene expression controlling receptor-signaling pathways. Mouse models are useful for understanding the cooperation and consequence of mutational order in leukemogenesis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Alizee Taquet, Helene Jourdan-Pineau, Christophe Simiand, Martial Grondin, Benoit Barres, Helene Delatte
Summary: The success of species invasion in agrosystems is affected by factors such as insecticide use, species evolutionary capabilities, and interactions with resident species. The invasions of MEAM1 and MED whitefly species have led to increased insecticide use and challenges for resident species on La Reunion Island. The study genotyped 41 populations and found that MEAM1 predominantly existed in agrosystems with a resistant kdr allele, while 10 mainly existed in natural environments without any resistant allele. Hybridization between the two species was detected in low frequency, but resistant alleles did not introgress into the resident species. MED showed limited distribution in agrosystems, but all individuals displayed a resistant allele.
SCIENTIFIC REPORTS
(2022)
Article
Orthopedics
Katherine T. Best, Valentina Studentsova, Jessica E. Ackerman, Anne E. C. Nichols, Marlin Myers, Justin Cobb, Emma Knapp, Hani A. Awad, Alayna E. Loiselle
Summary: This study investigated the effects of tamoxifen on tendon homeostasis and healing in mice. The results showed that tamoxifen had no effect on tendon healing in male mice, while it significantly decreased Max load in female repairs. Additionally, tamoxifen treatment led to disruptions in tendon homeostasis in female mice compared to corn oil treatment.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Oncology
Hee J. Kim, Woo C. Noh, Seok J. Nam, Byeong-Woo Park, Eun S. Lee, Seock A. Im, Yong S. Jung, Jung H. Yoon, Sung S. Kang, Kyong H. Park, Soo-Jung Lee, Joon Jeong, Min H. Lee, Se H. Cho, Sung Y. Kim, Hyun-Ah Kim, Se-Hwan Han, Wonshik Han, Min H. Hur, Seonok Kim, Sei H. Ahn
Summary: In breast cancer patients, younger age, use of Adriamycin instead of taxanes, and shorter duration of chemotherapy are associated with a higher likelihood of menstruation restoration after 5 years of tamoxifen treatment.
EUROPEAN JOURNAL OF CANCER
(2021)
Article
Biochemistry & Molecular Biology
Jun Liu, Shuya Kasai, Yota Tatara, Hiromi Yamazaki, Junsei Mimura, Seiya Mizuno, Fumihiro Sugiyama, Satoru Takahashi, Tsubasa Sato, Taku Ozaki, Kunikazu Tanji, Koichi Wakabayashi, Hayato Maeda, Hiroki Mizukami, Yasuhiro Shinkai, Yoshito Kumagai, Hirofumi Tomita, Ken Itoh
Summary: This study found that GCN1 is involved in hepatic lipid metabolism in adult mice. Tamoxifen treatment caused weight loss, decreased liver weight, hepatic glycogen and lipid contents, blood glucose and non-esterified fatty acids, and visceral white adipose tissue weight in mice, while food intake and viability remained unchanged.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Energy & Fuels
Pierpaolo Napolitano, Irina Jimenez, Benjamin Pla, Carlo Beatrice
Summary: This paper proposes a knock detection strategy for heavy-duty natural gas engines based on a combination of knock sensors and combustion modeling. The results show that this method has better knock recognition capabilities compared to traditional methods based on in-cylinder pressure analysis, with the advantage of using only knock sensors.
Article
Biochemistry & Molecular Biology
Mohammed Amir Husain, Annick Vachon, Raphael Chouinard-Watkins, Milene Vandal, Frederic Calon, Melanie Plourde
Summary: The metabolism of docosahexaenoic acid (DHA) is different in APOE4 carriers, and it affects cognitive performance. There is a plasma-liver-brain axis of DHA in APOE4 mice, which is influenced by genotype and diet. APOE4 mice rely more on plasma DHA, especially in cognitive performance.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Article
Oncology
Lindsay J. Collin, Maret L. Maliniak, Deirdre P. Cronin-Fenton, Thomas P. Ahern, Kristina B. Christensen, Sinna P. Ulrichsen, Per Damkier, Stephen Hamilton-Dutoit, Rami Yacoub, Peer M. Christiansen, Henrik Toft Sorensen, Timothy L. Lash
Summary: The study found that HIF-1α expression is not directly associated with overall breast cancer recurrence, but may be related to early recurrence in patients with ER-negative breast cancer.
BREAST CANCER RESEARCH
(2021)
Article
Thermodynamics
Rafal Rogoz, Niranjan Miganakallu, Zhuyong Yang, Jeffrey D. Naber, Lukasz Jan Kapusta
Summary: Combustion knock is a key factor limiting the performance of spark-ignition engines. Most previous studies focused on the influence of knock suppression methods on engine parameters, while neglecting the changes in knock probability distribution. This study analyzed the effects of co-injected additives on the probability distribution of knock and found slight but systematic changes in distribution skewness and kurtosis.
ENERGY CONVERSION AND MANAGEMENT
(2022)
Article
Biochemical Research Methods
Baoying Yuan, Yuhan Chen, Zhifeng Wu, Li Zhang, Yuan Zhuang, Xiaomei Zhao, Hao Niu, Jason Chia-Hsien Cheng, Zhaochong Zeng
JOURNAL OF PROTEOME RESEARCH
(2019)
Article
Biology
Bao-ying Yuan, Yu-han Chen, Zhi-feng Wu, Yuan Zhuang, Gen-wen Chen, Li Zhang, Hai-ge Zhang, Jason Chia-Hsien Cheng, Qin Lin, Zhao-chong Zeng
RADIATION RESEARCH
(2019)
Article
Oncology
Yuan Zhuang, Bao-ying Yuan, Gen-wen Chen, Xiao-mei Zhao, Yong Hu, Wen-chao Zhu, Zhao-chong Zeng, Yi-xing Chen
FRONTIERS IN ONCOLOGY
(2019)
Article
Immunology
Elizabeth Robins, Ming Zheng, Qingshan Ni, Siqi Liu, Chen Liang, Baojun Zhang, Jian Guo, Yuan Zhuang, You-Wen He, Ping Zhu, Ying Wan, Qi-Jing Li
Summary: Recent research has revealed that CD4(+) and CD8(+) T cells in adaptive immunity can exhibit significant lineage plasticity, even undergoing fundamental lineage reprogramming. This shift in functional potential may suggest a new direction for HIV vaccine design.
CELLULAR & MOLECULAR IMMUNOLOGY
(2021)
Article
Cell Biology
Yuhan Chen, Baoying Yuan, Genwen Chen, Li Zhang, Yuan Zhuang, Hao Niu, Zhaochong Zeng
JOURNAL OF CELLULAR PHYSIOLOGY
(2020)
Article
Immunology
Annette Ko, Masashi Watanabe, Thomas Nguyen, Alvin Shi, Achouak Achour, Baojun Zhang, Xiaoping Sun, Qun Wang, Yuan Zhuang, Nan-ping Weng, Richard J. Hodes
JOURNAL OF IMMUNOLOGY
(2020)
Article
Genetics & Heredity
Qingshan Ni, Jianyang Zhang, Zihan Zheng, Gang Chen, Laura Christian, Juha Gronholm, Haili Yu, Daxue Zhou, Yuan Zhuang, Qi-Jing Li, Ying Wan
FRONTIERS IN GENETICS
(2020)
Article
Oncology
Paulina M. Wojnarowicz, Marta Garcia Escolano, Yun-Han Huang, Bina Desai, Yvette Chin, Riddhi Shah, Sijia Xu, Saurabh Yadav, Sergey Yaklichkin, Ouathek Ouerfelli, Rajesh Kumar Soni, John Philip, David C. Montrose, John H. Healey, Vinagolu K. Rajasekhar, William A. Garland, Jeremy Ratiu, Yuan Zhuang, Larry Norton, Neal Rosen, Ronald C. Hendrickson, Xi Kathy Zhou, Antonio Iavarone, Joan Massague, Andrew J. Dannenberg, Anna Lasorella, Robert Benezra
Summary: AGX51 targets ID proteins in cancer cells, inhibiting cell growth and enhancing ROS production, showing potential therapeutic effects for multiple cancers.
Article
Immunology
Danielle J. Dauphars, Ariana Mihai, Liuyang Wang, Yuan Zhuang, Michael S. Krangel
Summary: The study demonstrates that prior Tcrd rearrangement is essential for a diverse Tcra repertoire in mouse thymocytes. The utilization of Trav15-dv6 family V gene segments in Tcrd rearrangements imparts diversity in the Tcra repertoire through instigating the use of central and distal V-alpha segments. The study reveals the indispensable role of Tcrd recombination, particularly the Trav15-dv6 family, in generating a combinatorially diverse Tcra repertoire.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Jeremy J. Ratiu, William E. Barclay, Elliot Lin, Qun Wang, Sebastian Wellford, Naren Mehta, Melissa J. Harnois, Devon DiPalma, Sumedha Roy, Alejandra V. Contreras, Mari L. Shinohara, David Wiest, Yuan Zhuang
Summary: The study uncovers the role of transcription factor Zfp335 in regulating survival of post-beta-selection thymocytes via the cGAS/STING pathway, providing insights into an important regulatory mechanism in T cell development.
NATURE COMMUNICATIONS
(2022)
Article
Oncology
Xiaomei Zhao, Biao Wang, Yuan Zhuang, Shisuo Du, Zhaochong Zeng
Summary: This study investigates the immunomodulatory effects of different dose-fractionation schedules and finds that a single high-dose radiation schedule has a stronger antitumor effect. The study also reveals the involvement of the cGAS/STING signaling pathway in the immunomodulatory effects of single high-dose radiation.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2023)
Article
Immunology
Ariana Mihai, Sumedha Roy, Michael S. Krangel, Yuan Zhuang
Summary: The V(D)J recombination of antigen receptor loci is a highly regulated process in lymphocyte development. The recombination of Tcra gene occurs in CD4(+)CD8(+) double positive thymocytes and requires the Tcra enhancer (E & alpha;). E proteins, known regulators of DP thymocyte development, have three binding sites in E & alpha;. Mutants lacking one or two of the binding sites showed partial blockage at the positive selection stage of & alpha;& beta; T cell development, loss of germline transcription, and dysregulated gene rearrangement. The binding of E proteins to E & alpha; promotes Tcra repertoire diversity.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Qun Wang, Yen-Yu Lin, Baojun Zhang, Jianxuan Wu, Sumedha Roy, Jeremy J. Ratiu, Yanping Xu, Meifang Dai, Laura P. Hale, Yue Xiong, Qi-Jing Li, Yuan Zhuang
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Oncology
Yi-Xing Chen, Yuan Zhuang, Ping Yang, Jia Fan, Jian Zhou, Yong Hu, Wen-Chao Zhu, Jing Sun, Zhao-Chong Zeng
TECHNOLOGY IN CANCER RESEARCH & TREATMENT
(2020)
Article
Oncology
Li Zhang, Li Yan, Hao Niu, Jie Ma, Bao-Ying Yuan, Yu-Han Chen, Yuan Zhuang, Yong Hu, Zhao-Chong Zeng, Zuo-Lin Xiang