Article
Multidisciplinary Sciences
Gloria S. Lee, Jiajing Zhang, Yuying Wu, Yi Zhou
Summary: Research shows that 14-3-3 proteins regulate the synaptic localization of NMDARs in neurons, promoting the surface delivery of NMDAR subunits, which is associated with the progression of symptoms of schizophrenia.
Article
Biochemistry & Molecular Biology
Jane Melissa Lim, Rumi Lee, Yeonsil Kim, In Young Lee, Eunju Kim, Eui-Ju Choi
Summary: This study demonstrates that excessive activation of NMDA receptor leads to excitotoxic neuronal death by increasing calcium influx. The cleavage and activation of MST1 by calpain 1, mediated by NMDA, promotes excitotoxicity in mouse cortical neurons.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Gareth M. Rurak, Stephanie Simard, Moises Freitas-Andrade, Baptiste Lacoste, Francois Charih, Amanda Van Geel, John Stead, Barbara Woodside, James R. Green, Gianfilippo Coppola, Natalina Salmaso
Summary: This study examines the sex differences and developmental changes in cortical astroglial cells. The results reveal two distinct phenotypes of astroglia during early and late development, while sex differences are mainly reflected in gene expression patterns during development.
Article
Chemistry, Multidisciplinary
Fengzhen Cui, Qingfei Pan, Siyi Wang, Faming Zhao, Runxin Wang, Tingting Zhang, Yaying Song, Jun He, Haolin Zhang, Qiang Weng, Yang Jin, Wei Xia, Yuanyuan Li, Guo-Yuan Yang, Winnok H. De Vos, Jean-Pierre Timmermans, Shunqing Xu, Yaohui Tang, Xia Sheng
Summary: The study reveals the neurodevelopmental toxicity of benzophenones during pregnancy, which impairs hippocampal development and causes cognitive dysfunction in offspring mice by activating specific signaling pathways that lead to inflammation and cell apoptosis.
Article
Biochemical Research Methods
Fuqing Jiang, Xin Zhou, Yingying Qian, Miao Zhu, Li Wang, Zhuxia Li, Qingmei Shen, Minhan Wang, Fangfang Qu, Guizhong Cui, Kai Chen, Guangdun Peng
Summary: The study describes a microfluidic indexing-based spatial assay (MISAR-seq) that combines spatial-ATAC-seq and RNA-seq to simultaneously profile chromatin accessibility and gene expression in the developing mouse brain. This method provides insights into tissue organization and spatiotemporal regulatory logics during brain development.
Article
Multidisciplinary Sciences
Erdyni N. Tsitsikov, Khanh P. Phan, Yufeng Liu, Alla V. Tsytsykova, Mike Kinter, Lauren Selland, Lori Garman, Courtney Griffin, Ian F. Dunn
Summary: TRAF7 is a crucial molecule involved in the shear stress-induced MEKK3-MEK5-ERK5 signaling pathway in endothelial cells. It associates with SCRIB and may be an upstream component of the signaling pathway.
Article
Multidisciplinary Sciences
Hakan Ozadam, Tori Tonn, Crystal M. Han, Alia Segura, Ian Hoskins, Shilpa Rao, Vighnesh Ghatpande, Duc Tran, David Catoe, Marc Salit, Can Cenik
Summary: Translation regulation is crucial for early mammalian embryonic development. However, the previous bulk measurements have limited the precise determination of translation regulation, including allele-specific analyses. In this study, a new microfluidic isotachophoresis (ITP) approach, called Ribo-ITP, was developed to profile translation in single oocytes and embryos during early mouse development. The results revealed differential translation efficiency as an important mechanism for regulating genes involved in centrosome organization and N-6-methyladenosine modification of RNAs. It also led to the discovery of stage-specific differential engagement of zygotic RNAs with ribosomes and reduced translation efficiency of transcripts exhibiting allele-biased expression. Moreover, the Ribo-ITP approach has the potential to enable various applications by providing high-coverage and high-resolution ribosome occupancy measurements from ultra-low input samples, including single cells.
Article
Cell Biology
Ya-ru Huang, Xi-xiu Xie, Jing Yang, Xiao-ying Sun, Xiao-yun Niu, Cheng-gang Yang, Ling-jie Li, Lun Zhang, Dan Wang, Chun-yu Liu, Sheng-jie Hou, Chen-yang Jiang, Yu-ming Xu, Rui-tian Liu
Summary: In this study, it was found that the levels of ArhGAP11A significantly increase in patients with AD and APP/PS1 mice. By reducing ArhGAP11A level in neurons, both AD generation and ADo neurotoxicity can be inhibited through the RhoA/ROCK/Erk signaling pathway. Furthermore, specific reduction of ArhGAP11A level significantly reduces AD production, plaque deposition, and improves neuronal damage, neuroinflammation, and cognitive deficits in APP/PS1 mice.
Article
Biochemistry & Molecular Biology
Minako Yokoyama, Toshitsugu Fujita, Yuka Kadonosawa, Yota Tatara, Daisuke Motooka, Masahito Ikawa, Hodaka Fujii, Yoshihito Yokoayama
Summary: CBR1 is a nicotinamide adenine dinucleotide phosphate (NADPH)-dependent reductase with broad substrate specificity. This study generated transgenic mice overexpressing CBR1 and characterized the expression of CBR1 in various organs, as well as identified changes in protein expression patterns. The transgenic mice may be useful for further understanding the molecular mechanisms regulated by CBR1 and its effects on carcinogenesis and cardiotoxicity of certain cancer drugs.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biology
Miguel Ramirez, Yuliya Badayeva, Joanna Yeung, Joshua Wu, Ayasha Abdalla-Wyse, Erin Yang, Brett Trost, Stephen W. Scherer, Daniel Goldowitz
Summary: This study identified active enhancers in the mouse cerebellum during embryonic and postnatal stages, revealing dynamic gene expression regulation during cerebellar development. The enhancers were enriched for neural transcription factor binding sites and showed cell-type specific expression regulation.
Article
Cell Biology
Yun Lu, Xiangyu Li, Kai Zhao, Yuanxin Shi, Zhengdong Deng, Wei Yao, Jianming Wang
Summary: This study identified novel protein kinases associated with cholangiocarcinoma (CCA) and demonstrated the effectiveness of the pan-inhibitor of the PKD family, 1-naphthyl PP1 (1-NA-PP1), in inhibiting the proliferation, migration, and invasion ability of CCA cells. These findings provide new therapeutic targets for CCA.
Article
Multidisciplinary Sciences
Yu Guo, Minjie Shen, Qiping Dong, Natasha M. Mendez-Albelo, Sabrina X. Huang, Carissa L. Sirois, Jonathan Le, Meng Li, Ezra D. Jarzembowski, Keegan A. Schoeller, Michael E. Stockton, Vanessa L. Horner, Andre M. M. Sousa, Yu Gao, Jon E. Levine, Daifeng Wang, Qiang Chang, Xinyu Zhao, Ian A. Glass, Dan Doherty
Summary: The study shows that the absence of FMRP leads to an increase in MAP1B, which inhibits neuronal development and social behavior. It also reduces autophagosome formation. Lowering MAP1B levels and activating autophagy can rescue the neuronal deficits in ASD and FXS.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Xue-Yan Zhang, Jun Li, Cai-Juan Li, Ying-Qi Lin, Chun-Hui Huang, Xiao Zheng, Xi-Chen Song, Zhu-Chi Tu, Xiao-Jiang Li, Sen Yan
Summary: The developmental and maturation pace of primary cerebral cortical neurons from cynomolgus monkeys is slower compared to mice, and cultured monkey cortical neurons exhibit typical features of Huntington's disease when infected with a mutant HTT. This suggests that cultured cynomolgus monkey neurons may serve as a cell model for studying human neurodegenerative diseases and neuronal regeneration.
NEURAL REGENERATION RESEARCH
(2021)
Article
Multidisciplinary Sciences
Wenxian Wang, Hyeyoung Cho, Jae W. Lee, Soo-Kyung Lee
Summary: This study investigates the role of the histone H3-lysine 27 demethylase Kdm6b in motor neuron subtype generation during spinal cord development in mice. The study reveals that Kdm6b promotes the generation of specific motor neuron subtypes while inhibiting others. Single-cell RNA-sequencing analysis shows the heterogeneity of these motor neuron subtypes, and the study suggests that Kdm6b acquires cellular fate specificity through interaction with the transcription factor complex Isl1-Lhx3.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Jinxi Li, James D. Glover, Haiguo Zhang, Meifang Peng, Jingze Tan, Chandana Basu Mallick, Dan Hou, Yajun Yang, Sijie Wu, Yu Liu, Qianqian Peng, Shijie C. Zheng, Edie Crosse, Alexander Medvinsky, Richard A. Anderson, Helen Brown, Ziyu Yuan, Shen Zhou, Yanqing Xu, John P. Kemp, Yvonne Y. W. Ho, Danuta Z. Loesch, Lizhong Wang, Yingxiang Li, Senwei Tang, Xiaoli Wu, Robin G. Walters, Kuang Lin, Ruogu Meng, Jun Lv, Jonathan M. Chernus, Katherine Neiswanger, Eleanor Feingold, David M. Evans, Sarah E. Medland, Nicholas G. Martin, Seth M. Weinberg, Mary L. Marazita, Gang Chen, Zhengming Chen, Yong Zhou, Michael Cheeseman, Lan Wang, Li Jin, Denis J. Headon, Sijia Wang
Summary: This study conducted genome-wide scans in Han Chinese cohorts and identified 18 loci associated with fingerprint type, as well as a genetic basis for the recognized pattern-block correlations. Additionally, a variant near EVI1 was found to alter regulatory activity and play a role in dermatoglyph patterning. Trans-ethnic meta-analysis identified 43 fingerprint-associated loci and revealed a genetic correlation between fingerprint patterns and hand proportions. These findings highlight the importance of limb development genes in shaping fingerprint patterning.
Article
Gastroenterology & Hepatology
Shuya Zhang, Huan Liu, Meimei Yin, Xiuying Pei, Angelika Hausser, Eri Ishikawa, Sho Yamasaki, Zheng Gen Jin
Article
Oncology
Wolfgang S. Lieb, Cristiana Lungu, Raluca Tamas, Hannah Berreth, Philipp Rathert, Peter Storz, Monilola A. Olayioye, Angelika Hausser
INTERNATIONAL JOURNAL OF CANCER
(2020)
Article
Biochemistry & Molecular Biology
Ines Mueller, Elwira Strozyk, Sebastian Schindler, Stefan Beissert, Htoo Zarni Oo, Thomas Sauter, Philippe Lucarelli, Sebastian Raeth, Angelika Hausser, Nader Al Nakouzi, Ladan Fazli, Martin E. Gleave, He Liu, Hans-Uwe Simon, Henning Walczak, Douglas R. Green, Jiri Bartek, Mads Daugaard, Dagmar Kulms
Article
Cell Biology
Natalia Pacheco-Fernandez, Mehrshad Pakdel, Birgit Blank, Ismael Sanchez-Gonzalez, Kathrin Weber, Mai Ly Tran, Tobias Karl-Heinz Hecht, Renate Gautsch, Gisela Beck, Franck Perez, Angelika Hausser, Stefan Linder, Julia von Blume
JOURNAL OF CELL BIOLOGY
(2020)
Correction
Biochemistry & Molecular Biology
Birte Zurek, Kornelia Ellwanger, Lisenka E. L. M. Vissers, Rebecca Schule, Matthis Synofzik, Ana Topf, Richarda M. de Voer, Steven Laurie, Leslie Matalonga, Christian Gilissen, Stephan Ossowski, Peter A. C. 't Hoen, Antonio Vitobello, Julia M. Schulze-Hentrich, Olaf Riess, Han G. Brunner, Anthony J. Brookes, Ana Rath, Gisele Bonne, Gulcin Gumus, Alain Verloes, Nicoline Hoogerbrugge, Teresinha Evangelista, Tina Harmuth, Morris Swertz, Dylan Spalding, Alexander Hoischen, Sergi Beltran, Holm Graessner
EUROPEAN JOURNAL OF HUMAN GENETICS
(2021)
Editorial Material
Biochemistry & Molecular Biology
Birte Zurek, Kornelia Ellwanger, Lisenka E. L. M. Vissers, Rebecca Schuele, Matthis Synofzik, Ana Topf, Richarda M. de Voer, Steven Laurie, Leslie Matalonga, Christian Gilissen, Stephan Ossowski, Peter A. C. 't Hoen, Antonio Vitobello, Julia M. Schulze-Hentrich, Olaf Riess, Han G. Brunner, Anthony J. Brookes, Ana Rath, Gisele Bonne, Gulcin Gumus, Alain Verloes, Nicoline Hoogerbrugge, Teresinha Evangelista, Tina Harmuth, Morris Swertz, Dylan Spalding, Alexander Hoischen, Sergi Beltran, Holm Graessner
Summary: Solve-RD is a groundbreaking EU project that brings together rare disease experts to actively share and analyze patient data, aiming to solve unsolved rare diseases through innovative clinical research methods. The project has already seen success in diagnosing cases through massive data re-analysis and novel -omics approaches.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2021)
Article
Cell Biology
Heidrun Steinle, Kornelia Ellwanger, Nora Mirza, Selina Briese, Ioannis Kienes, Jens Pfannstiel, Thomas A. Kufer
Summary: The study identified the molecular composition of the complexes formed by RIPK2 with several cellular proteins upon NOD1/2 activation. These interactions were validated using protein binding assays and immunofluorescence staining. Besides bacterial activation of NOD1/2, RIPK2 cytosolic complexes were also formed following XIAP inhibition and treatment with RIPK2 inhibitors.
JOURNAL OF CELL SCIENCE
(2021)
Article
Cell Biology
Carlos O. Oueslati Morales, Attila Ignacz, Norbert Bencsik, Zsofia Sziber, Aniko Erika Ratkai, Wolfgang S. Lieb, Stephan A. Eisler, Attila Szucs, Katalin Schlett, Angelika Hausser
Summary: The PKD family promotes AMPAR endocytosis in primary hippocampal neurons, affecting synaptic plasticity. Inhibition of PKD increases AMPAR levels at synapses, slows down their endocytic trafficking, and enhances neuronal network activity. Conversely, ectopic expression of constitutive active PKD reduces synaptic AMPAR levels but increases their colocalization with early endosomes.
Article
Cell Biology
Cathrin Hagenlocher, Robin Siebert, Bruno Taschke, Senait Wieske, Angelika Hausser, Markus Rehm
Summary: Prolonged ER stress can trigger cell death, but the mechanism differs between cancer cells and pancreatic beta cells. This study found that ER stress-induced apoptosis in pancreatic beta cells does not involve TRAIL-R2, unlike in cancer cells. These findings have implications for targeted treatment strategies that need to consider the ER stress susceptibility of different cell types.
CELL DEATH DISCOVERY
(2022)
Article
Multidisciplinary Sciences
Ronja Reinhardt, Kai Hirzel, Gisela Link, Stephan A. Eisler, Tanja Hagele, Matthew A. H. Parson, John E. Burke, Angelika Hausser, Thomas A. Leonard
Summary: Phosphorylation is a common mechanism in cell signaling, and protein kinases are often regulated by phosphorylation. However, PKD regulates itself through dimerization-mediated trans-autoinhibition, followed by autophosphorylation, to control its activity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Attila Ignacz, Domonkos Nagy-Herczeg, Angelika Hausser, Katalin Schlett
Summary: The actin cytoskeleton is the main regulator of synapse formation and maintenance. Different actin probes were compared in cultured hippocampal neurons to study their effectiveness in visualizing actin dynamics. The results showed that LifeAct-GFP and AC-GFP had similar affinity to actin, but they couldn't detect subtle changes in actin remodeling as effectively as EGFP-actin. Additionally, LifeAct-GFP had lower enrichment in dendritic spines compared to the other probes.
MOLECULAR BIOLOGY OF THE CELL
(2023)
Article
Oncology
Sophia Steinlein, Frank Essmann, Amanda Franceschini Ghilardi, Heike Horn, Julia Schueler, Angelika Hausser, Lijun Sun, German Ott, Claudia Kalla
Summary: In this study, two indolyl-chalcones (CIT-026 and CIT-223) were found to be highly effective in inhibiting the growth and viability of malignant pleural mesothelioma (MPM) cells, while having minimal effects on normal fibroblasts. These compounds induce tumor cell apoptosis by disrupting microtubule assembly and show promising potential as small-molecule therapeutics for MPM, especially in cells resistant to standard therapeutics.
FRONTIERS IN ONCOLOGY
(2023)
Review
Cell Biology
Elena Gutierrez-Galindo, Zeynep Hazal Yilmaz, Angelika Hausser
Summary: Protein kinase D (PKD) is a serine/threonine kinase family that plays a crucial role in the secretory pathway at the trans-Golgi network. Abnormal expression of PKD isoforms is mainly found in breast cancer, where it promotes growth, invasion, survival, and stem cell maintenance. This review discusses the isoform-specific functions of PKD in breast cancer progression, specifically focusing on the connection between PKD-regulated cellular processes and deregulated membrane trafficking and secretion. The challenges of targeting PKD as a therapeutic approach to prevent breast cancer progression are also highlighted.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
David Lagorce, Emeline Lebreton, Leslie Matalonga, Oscar Hongnat, Maroua Chahdil, Davide Piscia, Ida Paramonov, Kornelia Ellwanger, Sebastian Koehler, Peter Robinson, Holm Graessner, Sergi Beltran, Caterina Lucano, Marc Hanauer, Ana Rath
Summary: Rare diseases are difficult to diagnose, especially when the causative gene is unidentified. The Solve-RD project aims to identify the molecular causes of undiagnosed rare diseases through phenotypic similarity analysis and genomic data reanalysis. Promising results were obtained in the tested cases.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Biology
Kornelia Ellwanger, Selina Briese, Christine Arnold, Ioannis Kienes, Valentin Heim, Ueli Nachbur, Thomas A. Kufer
LIFE SCIENCE ALLIANCE
(2019)