Article
Engineering, Biomedical
Jiabing Fan, Chung-Sung Lee, Soyon Kim, Xiao Zhang, Joan Pi-Anfruns, Mian Guo, Chen Chen, Matthew Rahnama, Jiong Li, Benjamin M. Wu, Tara L. Aghaloo, Min Lee
Summary: The study found that Trb3 promotes osteoblastic commitment of MSCs while inhibiting adipocyte differentiation, regulating cell-fate choice through BMP/Smad and Wnt/beta-catenin signals. Local delivery of Trb3 using a GelCA-PLGA scaffold stimulated bone regeneration and inhibited fat-filled cyst formation, demonstrating a potential therapeutic strategy for improved skeletal regeneration and treatment of bone-loss disease.
Article
Biology
Rui Liu, Ting Zhu, Xin Chen, Zi Wang, Zhengyan Yang, Ang Ren, Liang Shi, Hanshou Yu, Mingwen Zhao
Summary: This study reveals that GSNOR regulates secondary metabolite production in microorganisms through S-nitrosylation of target proteins, specifically catalase. The findings shed light on the physiological functions of S-nitrosylated proteins and provide insights into the mechanism by which GSNOR responds to stress.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zun Wang, Xiaohua Li, Junxiao Yang, Yun Gong, Huixi Zhang, Xiang Qiu, Ying Liu, Cui Zhou, Yu Chen, Jonathan Greenbaum, Liang Cheng, Yihe Hu, Jie Xie, Xucheng Yang, Yusheng Li, Martin R. Schiller, Yiping Chen, Lijun Tan, Si-Yuan Tang, Hui Shen, Hong-Mei Xiao, Hong-Wen Deng
Summary: This study utilized single-cell RNA sequencing to identify distinct subpopulations of BM-MSCs in humans, shedding light on their cellular heterogeneity and roles in maintaining bone homeostasis, as well as potential novel markers for BM-MSC purification.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Cheng-Fong Chen, Yi-Chun Chen, Yu-Show Fu, Shang-Wen Tsai, Po-Kuei Wu, Chao-Ming Chen, Ming-Chau Chang, Wei-Ming Chen
Summary: This study explored the potential of revitalizing human decellularized allogeneic bone with human mesenchymal stem cells for osteochondral regeneration. Results showed that bone marrow and adipose tissue-derived MSCs exhibited higher osteogenic potential, while Wharton's jelly-derived MSCs had higher chondrogenic potential. Chondrogenic MSCs-seeded allogeneic bone may serve as potential osteochondral constructs for regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Priyanka Babuta, Kumar Paritosh, Renu Deswal
Summary: S-nitrosylation is a known post-translational modification that regulates nitric oxide-dependent cell signaling. NADH-dependent S-nitrosoglutathione reductase (GSNOR) and NADPH-dependent thioredoxin reductase (NTR) enzymes play important roles in maintaining nitric oxide/S-nitrosothiol (NO/SNO) homeostasis. The identification and analysis of GSNOR and NTR genes in Brassica juncea revealed their structural characteristics, evolutionary relationships, and subcellular localizations. Additionally, their activities were found to be regulated by light and plant hormones, and showed differential patterns in various tissues and developmental stages.
PLANT BIOTECHNOLOGY REPORTS
(2023)
Article
Multidisciplinary Sciences
Jianfang Wang, Qiaoling Zhu, Dandan Cao, Qiqi Peng, Xiaoying Zhang, Chong Li, Chen Zhang, Bo O. Zhou, Rui Yue
Summary: This study identifies bone marrow stromal cells (BMSCs) and megakaryocytes/platelets (MKs/PLTs) as two important sources of IGF-1 that coordinate to maintain and regenerate the adult skeleton, highlighting reciprocal regulation between the hematopoietic and skeletal systems.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell & Tissue Engineering
Yuan Zhu, Xiao Zhang, Kunkun Yang, Yuzi Shao, Ranli Gu, Xuenan Liu, Hao Liu, Yunsong Liu, Yongsheng Zhou
Summary: Research shows that macrophage-derived apoptotic vesicles (apoVs) can regulate the osteogenesis and adipogenesis of MSCs by delivering miR155. This study provides new insights and mechanisms for MSC-mediated tissue engineering.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Fatih Karadeniz, Jung Hwan Oh, Hyun Jin Jo, Jiho Yang, Hyunjung Lee, Youngwan Seo, Chang-Suk Kong
Summary: This study reported the anti-adipogenic effect of dracunculin (DCC) in human bone marrow-derived mesenchymal stromal cells (hBM-MSCs). DCC inhibits lipid accumulation and the expression of adipogenic transcription factors. The mechanism of action involves activating the beta-catenin pathway via AMPK. DCC has potential use as a nutraceutical against bone marrow adiposity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Judong Kim, S. M. Touhidul Islam, Fei Qiao, Avtar K. Singh, Mushfiquddin Khan, Jeseong Won, Inderjit Singh
Summary: The research shows that regulating the cytokines released by B cells can protect against neuroinflammatory diseases like experimental autoimmune encephalomyelitis. Using GSNO and GSNOR inhibitor N6022 can modulate IL-10 and IL-6, offering potential in treating autoimmune disorders.
Article
Endocrinology & Metabolism
Ziyi Li, Yafei Wang, Shilun Li, Yukun Li
Summary: Bone regeneration is a complex process involving the coordination of osteogenesis and osteoclastogenesis, with the balance between osteogenesis and adipogenesis of BMSCs playing a critical role. Recent studies have shown that exosomes from M2 macrophages facilitate osteogenesis and reduce adipogenesis through the miR-690/IRS-1/TAZ axis, potentially serving as a therapeutic tool for bone loss diseases.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Jung Hwan Oh, Fatih Karadeniz, Mi-Soon Jang, Hojun Kim, Youngwan Seo, Chang-Suk Kong
Summary: Loliolide shows potential as a functional food ingredient with anti-obesity properties. It can decrease lipid accumulation, suppress the expression of adipogenic transcription factors, and activate specific signaling pathways to inhibit adipogenesis.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Giovannamaria Petrocelli, Provvidenza Maria Abruzzo, Luca Pampanella, Riccardo Tassinari, Serena Marini, Elena Zamagni, Carlo Ventura, Federica Facchin, Silvia Canaider
Summary: This study investigated the effects of oxytocin on the proliferation, migratory ability, senescence, and autophagy of human adipose-derived stem cells (hASCs). The results showed that oxytocin did not affect hASC proliferation and migratory ability, but promoted autophagy and osteogenic differentiation. This suggests that oxytocin may promote osteogenesis in hASCs by modulating the autophagic process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mengran Qin, Lipeng Xing, Jiahan Wu, Shulei Wen, Junyi Luo, Ting Chen, Yaotian Fan, Jiahao Zhu, Lekai Yang, Jie Liu, Jiali Xiong, Xingping Chen, Canjun Zhu, Songbo Wang, Lina Wang, Gang Shu, Qingyan Jiang, Yongliang Zhang, Jiajie Sun, Qianyun Xi
Summary: Skeletal muscle-derived exosomes enriched with miR-146a-5p play a role in regulating adipogenesis and obesity by inhibiting the differentiation of preadipocytes and adipogenesis. The miR-146a-5p functions as a negative regulator of PPAR gamma signaling by targeting GDF5 gene, mediating adipogenesis and fatty acid absorption. These findings provide new insights into the skeletal muscle-fat signaling axis and suggest miR-146a-5p as a potential target for therapeutic interventions against metabolic diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Xinyun Xu, Ling Zhao, Paul D. Terry, Jiangang Chen
Summary: Mesenchymal stem cells from bone marrow can differentiate into either adipocytes or osteoblasts, and external stimuli such as environmental contaminants, heavy metals, dietary, and physical factors can influence this fate decision. Maintaining a balance between osteogenesis and adipogenesis is crucial for bone homeostasis, and disruptions in lineage commitment of these stem cells are associated with various bone disorders. This review focuses on understanding how external stimuli affect the fate of mesenchymal stem cells and calls for future studies to investigate the impact on bone health and underlying mechanisms of differentiation.
Article
Chemistry, Inorganic & Nuclear
Anna Wadolek, Maria Oszajca, Wiktoria Pecak, Malgorzata Brindell, Grazyna Stochel
Summary: S-Nitrosothiols act as a reservoir of releasable nitric oxide in vivo, and exposure to PM can affect their stability. Copper ions play a dominant role in inducing NO release from S-nitrosoalbumin, with the potential participation of other water-soluble PM components. Ascorbic acid enhances the effectiveness of NO release by PM extracts by promoting the reduction of Cu2+ to Cu+.
DALTON TRANSACTIONS
(2021)
Review
Endocrinology & Metabolism
Laszlo Hegedus, Antonio C. Bianco, Jacqueline Jonklaas, Simon H. Pearce, Anthony P. Weetman, Petros Perros
Summary: The treatment of hypothyroidism has not seen major innovations since the 1970s when thyroid extract was replaced by levothyroxine. The definition of subclinical hypothyroidism is debated and indiscriminate screening has led to overdiagnosis and treatment initiation at lower TSH levels. While levothyroxine substitution can improve quality of life, it may not always normalize it, especially for individuals with mild hypothyroidism.
NATURE REVIEWS ENDOCRINOLOGY
(2022)
Article
Endocrinology & Metabolism
Alexandra M. Dumitrescu, Erin C. Hanlon, Marilyn Arosemena, Olga Duchon, Matthew Ettleson, Mihai Giurcanu, Antonio C. Bianco
Summary: The study aimed to evaluate the pharmacokinetics and pharmacodynamics of PZL-derived T3 in healthy volunteers, showing that PZL exhibited more stable pharmacokinetic properties compared to LT3. This indicates the potential for PZL to provide hypothyroid patients with stable levels of serum T3.
Article
Endocrinology & Metabolism
Tatiana L. Fonseca, Samuel C. Russo, Cristina Luongo, Domenico Salvatore, Antonio C. Bianco
Summary: Inactivation of Dio3 in brown adipose tissue during embryonic development leads to premature exposure to thyroid hormone (T3) signaling, resulting in early adipocyte development and gene expression. BAT-D3KO mice show changes in weight, adipocyte area, and gene expression related to mitochondria, fat, and glucose homeostasis. However, their energy expenditure and ability to maintain core temperature remain normal.
Article
Endocrinology & Metabolism
Matthew D. Ettleson, Antonio C. Bianco, Wen Wan, Neda Laiteerapong
Summary: This study found that suboptimal treatment of hypothyroidism is associated with worse hospital outcomes, including longer length of stay and higher rates of readmission.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Cell Biology
Jinliang Li, Shannon C. Kelly, Jan R. Ivey, Pamela K. Thorne, Kelly P. Yamada, Tadao Aikawa, Renata Mazurek, James R. Turk, Kleiton Augusto Santos Silva, Amira R. Amin, Darla L. Tharp, Christina M. Mueller, Hrishikesh Thakur, Emily Leary, Timothy L. Domeier, R. Scott Rector, Kenneth Fish, Federico Cividini, Kiyotake Ishikawa, Craig A. Emter, Michael S. Kapiloff
Summary: This study assessed the biodistribution of self-complementary serotype 9 adeno-associated virus (AAV9sc) biologics in swine, demonstrating successful targeted delivery of cardiomyocyte-selective viral vectors without toxic effects. The results indicate the potential efficacy of these vectors in preclinical experimental settings.
PHYSIOLOGICAL GENOMICS
(2022)
Article
Multidisciplinary Sciences
Christopher Yanucil, Dominik Kentrup, Xueyi Li, Alexander Grabner, Karla Schramm, Eliana C. Martinez, Jinliang Li, Isaac Campos, Brian Czaya, Kylie Heitman, David Westbrook, Adam R. Wende, Alexis Sloan, Johanna M. Roche, Alessia Fornoni, Michael S. Kapiloff, Christian Faul
Summary: FGF21 targets cardiac myocytes to induce concentric hypertrophy, which may be a novel mechanism for heart failure in type 2 diabetes patients. Short-term elevation of FGF21 can be cardio-protective, but prolonged elevation may have adverse effects.
SCIENTIFIC REPORTS
(2022)
Article
Endocrinology & Metabolism
Richard Sinko, Petra Mohacsik, Dora Kovari, Veronika Penksza, Gabor Wittmann, Lilla Macsai, Tatiana L. Fonseca, Antonio C. Bianco, Csaba Fekete, Balazs Gereben
Summary: The study in THAI mice mimicking NTIS induced by infection or fasting showed different patterns of TH signaling in different tissues, suggesting that TH levels alone may not be a reliable marker of tissue TH signaling.
Article
Nursing
Fernanda de Oliveira Procopio, Erika Bevilaqua Rangel, Bartira de Aguiar Roza, Joao Roberto de Sa, Janine Schirmer
Summary: This study investigated medication adherence in type 2 diabetes mellitus among kidney transplant recipients and non-transplant recipients. The results showed that even though patients reported adherence to medication for diabetes control, their glycated hemoglobin levels were still above the recommended range. Additionally, no significant correlation was found between medication adherence, glycated hemoglobin, anxiety, and depression.
ACTA PAULISTA DE ENFERMAGEM
(2023)
Article
Behavioral Sciences
Julia Zaccarelli-Magalhaes, Gabriel R. Abreu, Andre R. Fukushima, Lorena P. Pantaleon, Beatriz B. Ribeiro, Camila Munhoz, Marianna Manes, Mayara A. de Lima, Julia Miglioli, Jorge C. Florio, Ivo Lebrun, Paula A. F. Waziry, Tatiana L. Fonseca, Barbara M. L. C. Bocco, Antonio C. Bianco, Esther L. Ricci, Helenice S. Spinosa
Summary: Postpartum depression is a common mental disorder that has negative effects on both women and children. By studying a rat model of postpartum depression caused by maternal separation, it was found that offspring displayed anxiety and poor maternal care due to the stress of separation. Female offspring also exhibited permanent impairment in sociability during adulthood.
BEHAVIOURAL BRAIN RESEARCH
(2023)
Article
Microbiology
Leticia Mello Bezinelli, Luciana Correa, Stephany Beyerstedt, Erika Bevilaqua Rangel, Carlos Benitez, Nelson Hamerschlak, Joao Renato Rebello Pinho, Debora Heller, Fernanda de Paula Eduardo
Summary: This study investigated the effectiveness of an oral hygiene protocol in reducing the SARS-CoV-2 viral load in the oral cavity. The results showed that using chlorhexidine (CHX) and hydrogen peroxide (HP) in the oral rinse immediately after the procedure significantly reduced the viral load.
JOURNAL OF ORAL MICROBIOLOGY
(2023)
Article
Ophthalmology
Ying Zhu, Julian Garcia-Sanchez, Roopa Dalal, Yang Sun, Michael S. Kapiloff, Jeffrey L. Goldberg, Wendy W. Liu
Summary: PIEZO1 and PIEZO2 are mechanosensitive ion channels that may play important roles in ocular function and intraocular pressure regulation. This study used single-molecule fluorescence in situ hybridization (smFISH) and transgenic reporter mice to compare the expression and localization of PIEZO1 and PIEZO2 in mouse ocular tissues relevant to glaucoma. The results suggest that PIEZO1 and PIEZO2 are differentially expressed and may have distinct roles as mechanosensors in glaucoma-relevant ocular tissues.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Medicine, General & Internal
Alexandre Veronese-Araujo, Debora D. de Lucena, Isabella Aguiar-Brito, Luis Gustavo Modelli de Andrade, Marina P. P. Cristelli, Helio Tedesco-Silva, Jose O. Medina-Pestana, Erika B. Rangel
Summary: Obesity is a component of the cardiometabolic syndrome that worsens the progression and mortality of COVID-19. In this study, the impact of overweight/obesity and kidney transplant on oxygen requirements in COVID-19 patients was investigated. The results showed that obesity was an independent risk factor for oxygen requirements in overweight/obese kidney transplant recipients, particularly in older individuals and smokers.
Meeting Abstract
Immunology
Lucia Alfaro Villanueva, Jose Medina-Pestana, Laila Viana, Marina Cristelli, Erika B. Rangel, Helio Tedesco-Silva, Roberto F. Meirelles
Article
Surgery
Erika B. Rangel, Debora D. de Lucena, Isabella Aguiar-Brito, Luis Gustavo Modelli de Andrade, Alexandre Veronese-Araujo, Marina P. Cristelli, Helio Tedesco-Silva, Jose O. Medina-Pestana
Summary: Kidney transplant recipients with pre-existing comorbidities, such as diabetes mellitus, are at higher risk of COVID-19 progression and mortality. This study found that fasting blood glucose, markers of inflammation (C-reactive protein) and tissue damage (lactate dehydrogenase) were associated with case fatality rate, invasive mechanical ventilation requirement, and acute kidney injury in kidney transplant patients affected by COVID-19. These factors were indicative of COVID-19 burden in this population, independently of diabetes mellitus status.
TRANSPLANT INTERNATIONAL
(2022)
Meeting Abstract
Surgery
L. A. Alfaro, L. A. Viana, M. P. Cristelli, E. B. Rangel, R. Meirelles, H. Tedesco, J. Medina
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)