Article
Biochemistry & Molecular Biology
Caroline Swoboda, Lena Deloch, Claudia von Zimmermann, Tanja Richter-Schmidinger, Bernd Lenz, Johannes Kornhuber, Christiane Muehle
Summary: This study investigated the potential of MIF at genetic, expression, and protein levels as a risk factor and biomarker for diagnosing, monitoring, or predicting MDD. The results suggest a genetic effect of MIF in women but do not provide strong evidence for its utility as a biomarker for MDD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Krishnamoorthi Sumaiya, Charles Solomon Akino Mercy, Gangatharan Muralitharan, Abdurahman Hajinur Hirad, Abdullah A. Alarfaj, Kalimuthusamy Natarajaseenivasan
Summary: This study evaluated the diagnostic value of macrophage migration inhibitory factor (MIF) for leptospirosis. The results showed that serum MIF levels were significantly elevated in leptospirosis patients and correlated with disease severity and progression. Serum MIF exhibited high sensitivity and specificity, making it a potential rapid diagnostic marker for leptospirosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jianbo He, Lin Zheng, Xiaojuan Li, Furong Huang, Sitao Hu, Lei Chen, Manya Jiang, Xianfeng Lin, Haibo Jiang, Yifan Zeng, Tianshen Ye, Dingkun Lin, Qian Liu, Jiake Xu, Kai Chen
Summary: This study aimed to explore the therapeutic potential of Obacunone (OB) on osteoporosis and its rudimentary mechanisms. In vitro and in vivo experiments showed that OB suppressed the formation and function of osteoclasts. Mechanistically, OB interacted with macrophage migration inhibitory factor (MIF), attenuating receptor activator of nuclear factor kappa B (NF-KB) ligand (RANKL)-induced signaling pathways. This led to a decrease in the expression level of the master transcriptional factor of osteoclastogenesis, nuclear factor of activated T cells 1 (NFATc1), and its downstream osteoclast-specific proteins. OB also alleviated estrogen deficiency-induced osteoporosis by targeting MIF and inhibiting hyperactive osteoclasts.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Cardiac & Cardiovascular Systems
Yan-yan Li, Hui Wang, Yang-yang Zhang
Summary: The MIF gene rs755622 G/C polymorphism is significantly associated with CAD, especially in the Chinese population. Individuals carrying the C allele of the MIF gene rs755622 G/C polymorphism may have a higher susceptibility to CAD.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Immunology
Xiangwen Shi, Yipeng Wu, Haonan Ni, Mingjun Li, Baochuang Qi, Yongqing Xu
Summary: The expression level of macrophage migration inhibitory factor (MIF) is upregulated in osteomyelitis patients and cell models. MIF inhibits osteogenic differentiation by activating the NF-κB signaling pathway. The study demonstrates that the MIF inhibitor iSO-1 and MIF knockdown can reverse the inhibitory effect of inflammation on osteogenic differentiation, providing a potential target for osteomyelitis treatment.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Chemistry, Analytical
Chenwen Shao, Christian Hedberg, Yong Qian
Summary: A novel activity-based probe, TPP2, was reported for direct labeling and imaging of endogenous MIF activity within live cells and in a mouse model of liver cancer. TPP2 allows for the dynamic monitoring of intracellular MIF activity and the identification of liver cancer with high sensitivity and selectivity, providing a promising new imaging approach for elucidating MIF-related biological functions in liver cancer.
ANALYTICAL CHEMISTRY
(2021)
Article
Endocrinology & Metabolism
Ni Cui, Hui Li, Yaoshan Dun, Jeffrey W. Ripley-Gonzalez, Baiyang You, Dezhao Li, Yuan Liu, Ling Qiu, Cui Li, Suixin Liu
Summary: This study found that hepatocyte-expressed macrophage migration inhibitory factor (MIF) can limit the occurrence of fatty liver during obesity. Exercise can reduce lipotoxicity and inhibit JNK activation by modulating endogenous hepatic MIF in nonalcoholic fatty liver disease (NAFLD). These findings have important clinical implications for the prevention and intervention of NAFLD induced by immoderate diet.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Zhangping Xiao, Shanshan Song, Deng Chen, Ronald van Merkerk, Petra E. van Der Wouden, Robbert H. Cool, Wim J. Quax, Gerrit J. Poelarends, Barbro N. Melgert, Frank J. Dekker
Summary: The development of a MIF-targeted PROTAC, MD13, is able to induce MIF degradation at low concentrations and suppress the proliferation of inflammatory and cancer cells, demonstrating the potential therapeutic value of PROTACs in cancer treatment.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Health Care Sciences & Services
Ioannis-Fivos Megas, David Simons, Bong-Sung Kim, Christian Stoppe, Andrzej Piatkowski, Panagiotis Fikatas, Paul Christian Fuchs, Jacqueline Bastiaanse, Norbert Pallua, Jurgen Bernhagen, Gerrit Grieb
Summary: This study observed 26 subjects undergoing microsurgical reconstruction, finding that blood levels of MIF, CRP, and leukocytes increased after surgery. Subjects requiring surgical revision due to microanastomosis thrombosis had significantly higher levels of MIF.
Article
Dermatology
Junhua Qi, Yu Zhang, Lvya Zhang, Gang Nie
Summary: This study conducted a meta-analysis to investigate the relationship between MIF-173G/C polymorphism and psoriasis risk. The results showed that carriers of the C allele and the GC genotype may have a higher risk of developing psoriasis.
INDIAN JOURNAL OF DERMATOLOGY VENEREOLOGY & LEPROLOGY
(2023)
Article
Agriculture, Multidisciplinary
Shaoshuai Liu, Maria Jose Ladera-Carmona, Minna M. Poranen, Aart J. E. van Bel, Karl-Heinz Kogel, Jafargholi Imani
Summary: The study suggests that MIF genes could be a potential target for insect control, but the application of naked dsRNA to plants for aphid control is inefficient. Focus should be put into developing effective dsRNA formulations for better pest management.
JOURNAL OF PLANT DISEASES AND PROTECTION
(2021)
Article
Clinical Neurology
Felix Neumaier, Christian Stoppe, Anzhela Stoykova, Miriam Weiss, Michael Veldeman, Anke Hoellig, Hussam Aldin Hamou, Yasin Temel, Catharina Conzen, Tobias Philip Schmidt, Rabia Dogan, Martin Wiesmann, Hans Clusmann, Gerrit Alexander Schubert, Roel Hubert Louis Haeren, Walid Albanna
Summary: This study determined the levels of macrophage migration inhibitory factor (MIF) in serum, cerebrospinal fluid (CSF), and cerebral microdialysate (MD) at different time points after aneurysmal subarachnoid hemorrhage (aSAH). The results showed that MIF levels in serum increased during the critical phase, while CSF levels remained relatively stable. MD levels peaked during the early phase, decreased during the critical phase, and increased again during the late phase. Subgroup analyses revealed higher serum and cerebral MIF levels in patients with anterior circulation aneurysms and those with delayed cerebral ischemia (DCI).
FRONTIERS IN NEUROLOGY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Markus Brandhofer, Juergen Bernhagen
Summary: In this study, Skeens et al. provide insights into the structure and dynamics of an oxidized form of the atypical cytokine macrophage migration-inhibitory factor (MIF). The research reveals the surprising conformational susceptibility of MIF to ambient redox alterations and identifies redox-sensitive residues and latent allostery sites with functional relevance.
Article
Oncology
Benjamin L. Woolbright, Ganeshkumar Rajendran, Erika Abbott, Austin Martin, Sarah Amalraj, Katie Dennis, Xiaogang Li, Joshua Warrick, John A. Taylor
Summary: MIF2 functions similarly to MIF1 in promoting bladder cancer development and progression, partly through CD74. Dual inhibition of MIF homologs is more effective in reducing tumor burden in bladder cancer compared to single inhibition of MIF1.
JOURNAL OF PATHOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Liu Yang, Deyin Guo, Chengpeng Fan
Summary: This study identified 24 dietary flavonoids that could inhibit the tautomerase activity of human macrophage migration inhibitory factor (MIF), with five of them showing lower IC50 values than the positive control ISO-1. X-ray crystallography, molecular docking, and cellular experiments were used to illustrate the molecular binding details and structure-activity relationships. Flavonoids were found to suppress cell chemotaxis and nitric oxide production in RAW264.7 cells, shedding light on potential lead compounds against MIF-related diseases.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Michael Rose, Vera Kloten, Erik Noetzel, Lukas Gola, Josef Ehling, Timon Heide, Steffen K. Meurer, Aljona Gaiko-Shcherbak, Antonio S. Sechi, Sebastian Huth, Ralf Weiskirchen, Oliver Klaas, Wiebke Antonopoulos, Qiong Lin, Wolfgang Wagner, Juergen Veeck, Felix Gremse, Julia Steitz, Ruth Knuechel, Edgar Dahl
Article
Biochemistry & Molecular Biology
Martin Schlensog, Lara Magnus, Timon Heide, Julian Eschenbruch, Florian Steib, Maximilian Tator, Vera Kloten, Michael Rose, Erik Noetzel, Nadine T. Gaisa, Ruth Knuechel, Edgar Dahl
Article
Biophysics
Gloria Fabris, Alessandro Lucantonio, Nico Hampe, Erik Noetzel, Bernd Hoffmann, Antonio DeSimone, Rudolf Merkel
BIOPHYSICAL JOURNAL
(2018)
Article
Oncology
Nuran Bektas Serce, Andreas Boesl, Irina Klaman, Sonja von Serenyi, Erik Noetzel, Michael F. Press, Arno Dimmler, Arndt Hartmann, Jalid Sehouli, Ruth Knuechel, Matthias W. Beckmann, Peter A. Fasching, Edgar Dahl
Article
Biochemistry & Molecular Biology
E. Noetzel, M. Rose, E. Sevinc, R-D Hilgers, A. Hartmann, A. Naami, R. Knuechel, E. Dahl
Article
Biochemistry & Molecular Biology
E. Noetzel, M. Rose, J. Bornemann, M. Gajewski, R. Knuechel, E. Dahl
Article
Multidisciplinary Sciences
Aljona Gaiko-Shcherbak, Gloria Fabris, Georg Dreissen, Rudolf Merkel, Bernd Hoffmann, Erik Noetzel
Article
Chemistry, Medicinal
Tabea Wiedenhoeft, Tobias Braun, Ronald Springer, Michael Teske, Erik Noetzel, Rudolf Merkel, Agnes Csiszar
Article
Biochemistry & Molecular Biology
Aljona Gaiko-Shcherbak, Julian Eschenbruch, Nils M. Kronenberg, Michael Teske, Benjamin Wolters, Ronald Springer, Malte C. Gather, Rudolf Merkel, Bernd Hoffmann, Erik Noetzel
Summary: The study found that disruption of the basement membrane can lead to the transition of benign acini to invasive, while EGFR activation further compromises the barrier function of the basement membrane, promoting cancer cell invasion. Breast acini continuously sense the mechanical properties of the extracellular matrix by forming new cellular protrusions, pushing and pulling on the basement membrane and extracellular matrix.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Michael Rose, Erik Noetzel, Jennifer Kistermann, Julian Eschenbruch, Sandra Rushrush, Lin Gan, Ruth Knuechel, Nadine T. Gaisa, Edgar Dahl
Summary: This study reveals that loss of ITIH5 in basal-type bladder cancer may inhibit cell growth and migration, and impact drug response via regulating the HA-CD44 axis.
Article
Cell Biology
Julian Eschenbruch, Georg Dreissen, Ronald Springer, Jens Konrad, Rudolf Merkel, Bernd Hoffmann, Erik Noetzel
Summary: The study reveals that actin microspikes are used as functional precursors for filopodia to initiate basement membrane penetration and widen collagen IV pores to anchor within the membrane scaffold via force-transmitting focal adhesions. Pre-invasive basal cells exhibit predominantly cortical actin networks and actin microspikes, which convert into highly contractile stress fibers in response to pro-invasive conditions. Ultimately, these EMT-related cytoskeletal adaptations are critical mechanisms inducing the invasive transition of benign breast acini.
Article
Engineering, Electrical & Electronic
Danyil Azarkh, Melanie Geiger, Se-Hyeong Jung, Erik Noetzel, Rudolf Merkel, Andrij Pich, Uwe Schnakenberg
Summary: In this study, a magnet measurement setup based on electromagnets was developed to characterize the physical material properties of soft materials. The deformations of ferrofluid droplets were measured to calculate viscosity and Young's modulus values. The setup can be used to investigate local and time-dependent rheological properties of soft materials.
SENSORS AND ACTUATORS A-PHYSICAL
(2022)
Article
Cell Biology
F. Friedland, S. Babu, R. Springer, J. Konrad, Y. Herfs, S. Gerlach, J. Gehlen, H. -j. Krause, L. De Laporte, R. Merkel, E. Noetzel
Summary: In this study, a magnetic shear strain device was developed to analyze the response of human breast gland cells to shear strain. The results showed that breast spheroids have resistance to shear strain, which increases with the maturation of the basement membrane and basoapical polarization. Interestingly, poorly developed spheroids were more prone to the extrusion of apoptotic cells under cyclic strain. This new device provides a versatile tool to study the response of 3D cell culture models to cyclic shear stress.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Lisann Esser, Ronald Springer, Georg Dreissen, Lukas Loevenich, Jens Konrad, Nico Hampe, Rudolf Merkel, Bernd Hoffmann, Erik Noetzel
Summary: Cell contractility regulates epithelial tissue geometry development and homeostasis. We developed an elastomeric pillar cage (EPC) array to quantify cell contractility as a mechanoresponse of epithelial microtissues to substrate stiffness and topography. This work introduced a versatile biophysical tool to explore mechanobiological regulation circuits driving low and high-tensional states during microtissue development and homeostasis.
Article
Materials Science, Biomaterials
Jiali Wang, Eric Platz-Baudin, Erik Noetzel, Andreas Offenhaeusser, Vanessa Maybeck
Summary: This study constructs three subcellularly-targeted optogenetic actuators based on mucin tandem repeats, and evaluates their expression and transfection efficiencies in different cell types. The results show that these actuators can be localized in both epithelial cells and neurons, opening up new possibilities for optogenetic manipulation in epithelial processes.