Article
Biotechnology & Applied Microbiology
Indira Sigdel, Awurama Ofori-Kwafo, Robert J. Heizelman III, Andrea Nestor-Kalinoski, Balabhaskar Prabhakarpandian, Amit K. Tiwari, Yuan Tang
Summary: This study presents a microfluidic tumor microenvironment (TME) model for evaluating the anti-metastatic efficacy of a novel thienopyrimidines analog with anti-cancer properties. The microfluidic device consists of a tissue compartment flanked by vascular channels, allowing for the co-culture of multiple cell types and providing a wide range of culturing conditions in one device.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Bozhil Robev, Ivan Iliev, Iana Tsoneva, Albena Momchilova, Alexandrina Nesheva, Aneliya Kostadinova, Galya Staneva, Biliana Nikolova
Summary: This study investigated the effects of Iscador Qu and Iscador M on various cellular processes in breast cancer cell lines. The results showed that Iscador Qu and M did not have any phototoxic effects. The antiproliferative activity of Iscador species was dose-dependent and varied among different cell lines based on their metastatic potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nanoscience & Nanotechnology
Afroditi Nanou, Ivan Lorenzo-Moldero, Kyriakos D. Gazouleas, Barbara Cortese, Lorenzo Moroni
Summary: Cancer biology research is shifting towards innovative in vitro 3D culture models to better mimic in vivo cancer biology. This study evaluated a 3D engineered system using porous scaffolds with two different porosities and compared it to conventional 2D cell culture. The results showed that static seeding was the optimal method and cells cultured in 3D scaffolds exhibited a more dormant phenotype.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Angela Ximena Argote Camacho, Amanda Rocio Gonzalez Ramirez, Alejandro Jose Perez Alonso, Juan David Rejon Garcia, Maria Auxiliadora Olivares Urbano, Pablo Torne Poyatos, Sandra Rios Arrabal, Maria Isabel Nunez
Summary: Early diagnosis is crucial in reducing the incidence and mortality rates of breast cancer. The study found that MMP-1 and MMP-3 are associated with the development of breast cancer and have the potential to be used as biomarkers for breast cancer diagnosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Maria-Elpida Christopoulou, Eleni Papakonstantinou, Daiana Stolz
Summary: Matrix metalloproteinases (MMPs) are proteolytic enzymes that regulate airway remodeling and may contribute to the development of emphysema in chronic obstructive pulmonary disease (COPD). This literature review examines the role of different MMPs in COPD, their regulation by tissue inhibitors, and the potential therapeutic targeting of MMPs in COPD. Recent clinical trials supporting this approach are also discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Shanshan Wang, Jiarui Xie, Xuanxuan Zou, Taotao Pan, Qichao Yu, Zhenkun Zhuang, Yu Zhong, Xin Zhao, Zifei Wang, Rui Li, Ying Lei, Jianhua Yin, Yue Yuan, Xiaoyu Wei, Longqi Liu, Shiping Liu, Huanming Yang, Liang Wu
Summary: This study investigates the molecular mechanisms underlying metastasis in hepatocellular carcinoma (HCC) using single-cell transcriptomic, proteomic, and chromatin accessibility data. The researchers found that the prevalence of a mesenchymal state and levels of cell proliferation are linked to metastatic potential in HCC cell lines. They also identified a rare hypoxic subtype with increased glycolysis capacity and higher metastatic potential. Furthermore, they identified a 14-gene panel representing the hypoxia signature, which could serve as a prognostic index.
Article
Multidisciplinary Sciences
Zoi Diamantopoulou, Francesc Castro-Giner, Fabienne Dominique Schwab, Christiane Foerster, Massimo Saini, Selina Budinjas, Karin Strittmatter, Ilona Krol, Bettina Seifert, Viola Heinzelmann-Schwarz, Christian Kurzeder, Christoph Rochlitz, Marcus Vetter, Walter Paul Weber, Nicola Aceto
Summary: The generation of circulating tumor cells (CTCs) with metastatic ability is concentrated during the rest phase, and CTCs generated during the active phase lack metastatic ability. Mechanistically, the upregulation of mitotic genes during the rest phase enables CTCs' metastasis proficiency. Circadian rhythm hormones such as melatonin, testosterone and glucocorticoids dictate the generation dynamics of CTCs, and insulin promotes tumor cell proliferation in a time-dependent manner.
Review
Oncology
Maximillian Viera, George Wai Cheong Yip, Han-Ming Shen, Gyeong Hun Baeg, Boon Huat Bay
Summary: Breast cancer-related deaths are often caused by metastasis. CD82, a known metastasis suppressor, could be a potential target for preventing metastases in breast cancer. Precision oncology offers a tailored treatment approach for specific groups of patients, and the restoration of CD82 levels could be achieved through existing chemotherapeutic drugs, CD82 peptide mimics, or non-coding RNA-based therapeutics.
Review
Pathology
Sabine Dieleman, Romy Aarnoutse, Janine Ziemons, Loes Kooreman, Annemarie Boleij, Marjolein Smidt
Summary: Breast cancer tissue has its own unique microbiota which can influence cancer initiation and progression, serving as a potential biomarker for treatment and prognosis. Variations in breast microbiota composition among cancer subtypes and disease severities may contribute to immunosuppression and evasion of immune destruction by tumor cells. The interactions between breast microbiota, gut microbiota, and the immune system offer potential targets for improving therapeutic efficacy.
AMERICAN JOURNAL OF PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Luis O. Gonzalez, Noemi Eiro, Maria Fraile, Rosario Sanchez, Alejandro Andicoechea, Silvia Fernandez-Francos, Jose Schneider, Francisco J. Vizoso
Summary: High-grade tumor budding is associated with a more aggressive tumor phenotype, and when combined with MMP/TIMP expression by stromal cells at the invasive front of the tumor, it helps identify patients with poor prognosis. The optimal cut-off for predicting both relapse-free and overall survival was found to be a tumor budding count >= 4. The study also showed that high-grade tumor budding was linked to MMP/TIMP expression by cancer-associated fibroblasts.
Article
Multidisciplinary Sciences
Peng Ji, Yue Gong, Ming-Liang Jin, Huai-Liang Wu, Lin-Wei Guo, Yu-Chen Pei, Wen-Jun Chai, Yi-Zhou Jiang, Yin Liu, Xiao-Yan Ma, Gen-Hong Di, Xin Hu, Zhi-Ming Shao
Summary: The response rates of immune checkpoint inhibitors in TNBC patients are limited, indicating the presence of additional immune escape mechanisms. By using customized in vivo CRISPR screens and transcriptomic analysis, Lgals2 was identified as a potential regulator involved in immune escape in TNBC. Mechanistic studies showed that Lgals2 induced the polarization and proliferation of tumor-associated macrophages through the CSF1/CSF1R axis, resulting in the immunosuppressive microenvironment of TNBC. Blockade of LGALS2 using an inhibitory antibody effectively halted tumor growth and reversed immune suppression.
Article
Oncology
D. Hodgson, Z. Lai, S. Dearden, J. C. Barrett, E. A. Harrington, K. Timms, J. Lanchbury, W. Wu, A. Allen, E. Senkus, S. M. Domchek, M. Robson
Summary: In this study, testing for BRCA1 and/or BRCA2 mutations in tumor tissue was found to be an additional route to inform treatment selection for PARP inhibition in patients with HER2-negative metastatic breast cancer. The high concordance between germline and tumor tissue testing, as well as the common occurrence of gsLOH, suggests biallelic inactivation in metastatic breast cancer. Furthermore, the efficacy of Olaparib was observed irrespective of gsLOH status or HRD score, indicating that additional tissue testing may not be necessary for treatment selection in these patients.
ANNALS OF ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Chitra Thakur, Yiran Qiu, Qian Zhang, Nicholas J. Carruthers, Miaomiao Yu, Zhuoyue Bi, Yao Fu, Priya Wadgaonkar, Bandar Almutairy, Akimasa Seno, Paul M. Stemmer, Fei Chen
Summary: This report provides evidence of diminished expression of the mineral dust-induced gene (mdig) in human triple negative breast cancer (TNBC). The study demonstrates that mdig promotes the growth of primary tumors but inhibits metastasis of TNBC cells. The findings also suggest a potential role of mdig in regulating genes related to cell motility, invasion, and metastasis.
Article
Oncology
Ruth M. Escalona, George Kannourakis, Jock K. Findlay, Nuzhat Ahmed
Summary: This study analyzed the expression of TIMPs and associated MMPs in different stages and grades of serous ovarian tumors, as well as their response to chemotherapy treatment. The results showed that TIMPs and MMPs are differentially expressed in ovarian tumors and are associated with cancer stem cells.
FRONTIERS IN ONCOLOGY
(2022)
Review
Medicine, General & Internal
Chiara Tommasi, Benedetta Pellegrino, Anna Diana, Marta Palafox Sancez, Michele Orditura, Mario Scartozzi, Antonino Musolino, Cinzia Solinas
Summary: The immune system plays an important role in neoplastic disease, but the role of innate immunity in cancer development and progression is still not well understood. The tumor microenvironment influences the terminal differentiation of innate immune cells, which can have either a pro-tumor or anti-tumor effect. Macrophages, natural killer cells, monocytes, dendritic cells, and neutrophils, along with elements of the complement system, are the key players of innate immunity in cancer development and evolution.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Obstetrics & Gynecology
Manoel Orlando Goncalves, Joao Siufi Neto, Marina Paula Andres, Daniela Siufi, Leandro Accardo de Mattos, Mauricio S. Abrao
Summary: DL was able to detect retrocervical, ovarian, and bladder endometriosis with similar sensitivity and specificity as TVUS-BP, whereas for vaginal and rectosigmoid endometriosis, DL had lower sensitivity and specificity than TVUS-BP. The study showed that TVUS-BP is an accurate tool for diagnosing ovarian and deep endometriosis, with higher sensitivity in detecting rectosigmoid endometriosis compared to DL.
HUMAN REPRODUCTION
(2021)
Review
Dentistry, Oral Surgery & Medicine
Daniel Isaac Sendyk, Nathalia Vilela Souza, Joao Batista Cesar Neto, Dimitris N. Tatakis, Claudio Mendes Pannuti
Summary: This study revealed a high prevalence of selective outcome reporting (SOR) in root coverage randomized clinical trials (RCTs), including downgrading of primary outcomes, upgrading of secondary outcomes, introduction of new primary outcomes in publications, omission of protocol primary outcomes in publications, and discrepancies in primary outcome timing. SOR was significantly associated with study significance and unclear outcome definitions in the publications.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2021)
Article
Obstetrics & Gynecology
Juliana Ribeiro da Silva, Marina Paula Andres, Ana Paula Klautau Leite, Maria Teresa Natel de Almeida Gomes, Joao Siufi Neto, Edmund Chada Baracat, Francisco Carmona, Mauricio Simoes Abrao
Summary: The study compared the sensitivity and specificity of TVUS narrative report with structured report for the diagnosis of adenomyosis. The structured report showed higher sensitivity and lower specificity compared to the narrative report.
JOURNAL OF MINIMALLY INVASIVE GYNECOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Carlos Guillermo Benitez Silva, Vitor Marques Sapata, Alexandre Hugo Llanos, Marcelo Munhoes Romano, Ronald Ernst Jung, Christoph Hans Franz Hammerle, Joao Batista Cesar-Neto, Claudio Mendes Pannuti, Giuseppe Alexandre Romito
Summary: In the aesthetic zone, tissue changes following alveolar ridge preservation (ARP) treatment using different biomaterials showed no significant differences in mid-facial mucosal level and marginal bone loss 1 year after implant restoration. However, soft tissue thickness at 3 and 5mm below the mucosal margin significantly differed between groups at pre-crown placement stage. After crown insertion, tissue stability was predictable regardless of biomaterial used.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2022)
Letter
Obstetrics & Gynecology
Mauricio S. Abrao, Marina Paula Andres, Joao Siufi Neto, Charles E. Miller, Julian A. Gingold, Mariona Rius, Francisco Carmona
JOURNAL OF MINIMALLY INVASIVE GYNECOLOGY
(2022)
Review
Cell & Tissue Engineering
Isaura Beatriz Borges Silva, Camila Harumi Kimura, Vitor Prado Colantoni, Mari Cleide Sogayar
Summary: Type 1 diabetes is characterized by the destruction of insulin-producing beta cells by the immune system. Current treatment strategies struggle to maintain glycemic control, leading to research into expanding beta-cell cultures for pancreatic islet replacement therapy using stem cell differentiation protocols. Challenges remain in efficiently producing functional beta cells for long-term euglycemia maintenance in clinical translation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Oncology
Augusto Pereira, Javier F. Magrina, Paul M. Magtibay, Joao Siufi Neto, Daniela F. S. Siufi, Yu-Hui H. Chang, Tirso Perez-Medina
Summary: Minimally invasive surgery can be used for interval debulking after neoadjuvant chemotherapy in selected patients with initially unresectable ovarian cancer. It has the main benefits of shorter hospital stay and fewer intestinal resections compared to primary debulking. Neoadjuvant chemotherapy allows a minimally invasive approach for interval debulking in patients with ovarian cancer considered unresectable at diagnosis.
Correction
Cell & Tissue Engineering
Isaura Beatriz Borges Silva, Camila Harumi Kimura, Vitor Prado Colantoni, Mari Cleide Sogayar
STEM CELL RESEARCH & THERAPY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Vanessa Camillo de Almeida, Claudio Mendes Pannuti, Marcelo Sirolli Ferreira, Rafael de Oliveira Lazarin, Giuseppe Alexandre Romito, Ronald Ernst Jung, Dimitris Nikolaos Tatakis, Cleverson de Oliveira e Silva, Joao Batista Cesar Neto
Summary: The purpose of this study was to compare the outcomes of a modified gingival graft technique with the conventional free gingival graft procedure for gingival augmentation. The results showed that both techniques were effective in increasing keratinized tissue width and thickness. However, the modified technique resulted in less postoperative pain and analgesic intake.
BRAZILIAN ORAL RESEARCH
(2023)
Editorial Material
Public, Environmental & Occupational Health
Mauricio S. Abrao, Joao Siufi Neto
Article
Biology
M. S. Moraes-Almeida, M. C. Sogayar, M. A. A. Demasi
Summary: This study aimed to investigate the synthetic lethality interaction between Chromodomain Helicase DNA binding proteins family member (CHD4) and histone methyltransferases family member (SETDB1) in triple negative breast cancer (TNBC) cells. Using CRISPR-Cas9 gene editing tool, indels were introduced in the genomic loci of SETDB1 and CHD4, and it was found that there exists a synthetic lethality interaction between these genes. These findings provide valuable insights for the development of new therapeutic approaches for not only TNBC but also other cancer types lacking molecular targets.
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
(2023)
Article
Biology
L. Andreone, A. F. dos Santos, R. A. M. Wailemann, L. F. Terra, V. M. Gomes, J. Macedo da Silva, L. Rosa-Fernandes, M. C. Sogayar, G. Palmisano, L. Labriola, M. J. Perone
Summary: This study investigates the use of skin spheroid-derived cells (SphCs) as a potential adjuvant for islet transplantation in type 1 diabetes. The researchers found that SphCs can improve the functionality of transplanted islets without the need for pharmacological immunosuppression.
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
(2023)
Review
Obstetrics & Gynecology
Fernanda Arcoverde, Marina P. Andres, Carolina C. Souza, Joao S. Neto, Mauricio S. Abrao
Summary: Deep endometriosis (DE) is an infiltrative disease that causes pain and infertility, with hormonal treatment being effective for 2/3 of patients and surgery improving quality of life in most cases. Medical treatment is preferred for uncomplicated DE, while surgery is recommended for those who do not respond to medical treatment or have visceral impairment.
MINERVA OBSTETRICS AND GYNECOLOGY
(2021)
Article
Obstetrics & Gynecology
Mauricio Simoes Abrao, Marina Paula Andres, Mariana Cunha Vieira, Giuliano Moyses Borrelli, Joao Siufi Neto
Summary: The study found that bowel endometriosis does not increase over time during reproductive age and reduces after menopause. Symptoms are also not related to the progression of the bowel lesion.
REPRODUCTIVE SCIENCES
(2021)
Article
Dentistry, Oral Surgery & Medicine
Yo-Wei Chen, Matthew Finkelman, Panos Papaspirisdakos, Joao Batista Cesar-Neto, Hans Peter Weber, Andre Barbisan de Souza
Summary: The study evaluated bone alterations following maxillary molar extraction using CBCT analysis and identified significant dimensional changes in both vertical and horizontal directions. The remodeling of the coronal aspect of the alveolar ridge played a major role in vertical changes.