Article
Dermatology
Zackie Aktary, Jeremy H. Raymond, Marie Pouteaux, Veronique Delmas, Valerie Petit, Lionel Larue
Summary: The establishment of consistent genetically modified mouse melanoma models and cell lines is crucial for prevention and treatment. This study reviews different mouse melanoma cell lines and explores the changes they undergo through molecular characterization and modification of the genome and microenvironment. These cell lines with defined genetic mutations enable the testing of innovative therapies and have relevance to human disease.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Article
Multidisciplinary Sciences
Xiong Shu, Weifeng Liu, Huiqi Liu, Hui Qi, Chengai Wu, Yu-Liang Ran
Summary: This study identified differential expression of miRNAs between CD133(+) and CD133(-) cells, with miR-4284 being a particularly interesting miRNA that may have significant roles in the function of OSCs, especially associated with the Wnt signaling pathway.
Article
Oncology
Mateusz Sikora, Katarzyna Krajewska, Klaudia Marcinkowska, Anna Raciborska, Rafal Jakub Wiglusz, Agnieszka Smieszek
Summary: Osteosarcoma is a malignant bone tumor that mainly affects children and elderly people. The primary treatment methods for osteosarcoma are still underdeveloped, leading to poor survival rates. This study aimed to establish the expression profile of molecular markers related to osteosarcoma survival, progression, and metastasis. The findings may facilitate the selection of reliable cellular models for pre-clinical investigations on osteosarcoma therapy development.
Article
Chemistry, Medicinal
Angela De Luca, Daniele Bellavia, Lavinia Raimondi, Valeria Carina, Viviana Costa, Milena Fini, Gianluca Giavaresi
Summary: Resveratrol (RSV) exhibits multiple tumor-suppressing activities on osteosarcoma (OS) cell lines, including inhibition of cell growth, promotion of cell differentiation, inhibition of cell migration, and enhancement of cytotoxic effect in combination with chemotherapy.
Article
Genetics & Heredity
Xiaoming Dong, Qiwei Yang, Zhenwu Du, Guizhen Zhang, Chuankai Shi, Xuyuan Qin, Yang Song
Summary: This study aimed to identify the optimal reference genes for gene expression analysis in osteosarcoma cell lines. Through standardization research, it was found that the combination of PUM1 and PPIA + ALAS1 was recommended as the best reference genes in these four different sources of human osteosarcoma cell lines.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Long Xie, Wenhua Yin, Fuxing Tang, Maolin He
Summary: This study investigated the functions of TERT in pan-cancer and osteosarcoma (OS) using multi-omics data analysis. The results showed that TERT expression was associated with various types of human cancer and DNA methylation, as well as immune cell infiltration in the tumor microenvironment. In vitro experiments demonstrated that inhibition of TERT reduced OS cell proliferation, migration, and metastasis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Shun-Cheng Yang, Hsuan-Ying Chen, Wan-Ling Chuang, Hui-Chun Wang, Cheng-Pu Hsieh, Yi-Fu Huang
Summary: Hinokitiol exhibits antitumor activities against osteosarcoma cells through inducing senescence in cells with wild-type p53 and apoptosis in cells with mutated p53. Additionally, it induces autophagy in both cell lines, and inhibition of autophagy enhances the senescence effect in U-2 OS cells and promotes apoptosis in MG-63 cells. The analysis of p53 status and co-administration of autophagy inhibitors may provide more precise and efficacious therapies in hinokitiol-related trials for treating osteosarcoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ya-Ting Yang, Vilma Yuzbasiyan-Gurkan
Summary: Osteosarcoma is a common bone tumor in humans and dogs, but treatment advances have been limited. A combination of doxorubicin and sorafenib has been found to have synergistic effects in both human and canine osteosarcoma. Therefore, clinical trials in dogs are warranted and can provide data support for human clinical trials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Radhika Saraf, Aniruddha Datta, Chao Sima, Jianping Hua, Rosana Lopes, Michael L. Bittner, Tasha Miller, Heather M. Wilson-Robles
Summary: Osteosarcoma is a challenging bone tumor to treat due to its complex nature and lack of understanding about its causes. This study combines existing literature on biological pathways in osteosarcoma with research on natural compounds to identify new targets and design more effective drug therapies. Through a Boolean network model and combinatorial circuit, the efficacy of various drugs in combination with Cryptotanshinone is predicted. The herbal drug Cryptotanshinone is shown to induce apoptosis in osteosarcoma cell lines by increasing sensitivity to TNF-related apoptosis-inducing ligand (TRAIL) through its actions on STAT3, DRP1, and DR5. The Boolean framework is used to identify additional drug intervention points in the pathway that could enhance the effects of Cryptotanshinone.
IEEE-ACM TRANSACTIONS ON COMPUTATIONAL BIOLOGY AND BIOINFORMATICS
(2022)
Article
Cell & Tissue Engineering
Hideki Izumi, Yuanyuan Li, Michio Yasunami, Seiji Sato, Takao Mae, Yasuhiko Kaneko, Akira Nakagawara
Summary: CD133 is a transmembrane protein mainly located on the plasma membrane of stem cells and cancer stem cells. The study demonstrates that CD133 plays a significant role in cell homeostasis by being degraded through selective autophagy and regulating autophagic activity. The asymmetric distribution of CD133 and nuclear beta-catenin during cell division induces a symmetry breaking of autophagic activity.
Article
Biochemistry & Molecular Biology
Dong-Wook Min, Hwang-Phill Kim, Jinhyun Kim, Xianyu Wen, Sungsik Kim, Young-Won Cho, Yoojoo Lim, Sang-Hyun Song, Sae-Won Han, Sunghoon Kwon, Gyeong Hoon Kang, Tae-You Kim
Summary: Tumor heterogeneity in colon cancer therapy poses challenges, with single cell analysis revealing heterogeneous subclones within CD133 positive cancer stem cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Oncology
Emilija Milosevic, Nemanja Stanisavljevic, Srdjan Boskovic, Nemanja Stamenkovic, Mirjana Novkovic, Alberto Bavelloni, Vittoria Cenni, Snezana Kojic, Jovana Jasnic
Summary: In this study, the antitumor activity of bacterial pigment violacein in osteosarcoma and rhabdomyosarcoma cell lines was evaluated. Violacein showed selective toxicity to tumor cells, inducing apoptosis and affecting cell migration. These findings further support the potential of violacein as an anticancer agent.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yumi Takashima, Seiji Yamamoto, Noriko Okuno, Takeru Hamashima, Son Tung Dang, Ngoc Dung Tran, Naruho Okita, Fujikawa Miwa, Thanh Chung Dang, Mina Matsuo, Keizo Takao, Toshihiko Fujimori, Hisashi Mori, Kazuyuki Tobe, Makoto Noguchi, Masakiyo Sasahara
Summary: The study examined the role of platelet-derived growth factor receptor alpha (PDGFRα) in the beiging of neonatal mice inguinal white adipose tissue (ingWAT). The deletion of PDGFRα suppressed ingWAT growth and increased the number and expression of beige adipocytes during the neonatal period. This plasticity of PDGFRα cells may contribute to the therapeutic remodeling of white adipose tissue for metabolic diseases.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biology
Kuan-Wei Chiu, Hsuan-Ying Chen, Chiu-Liang Chen, Cheng-Pu Hsieh, Yi-Fu Huang
Summary: This study demonstrates that carvacrol inhibits the proliferation of osteosarcoma cells and induces apoptosis. It also induces ER stress and increases the levels of reactive oxygen species, contributing to apoptosis. The findings suggest that targeting ER stress might enhance the antitumor activity of carvacrol in osteosarcoma cells.
Article
Veterinary Sciences
Cecilia Gola, Diana Giannuzzi, Andrea Rinaldi, Selina Iussich, Paola Modesto, Emanuela Morello, Paolo Buracco, Luca Aresu, Raffaella De Maria
Summary: The study aims to determine variants, putative driver genes, and gene expression aberrations in canine OSA cell lines, revealing a mean tumor mutational burden of 9.6 mutations/Mb. Known oncogenes and tumor suppressor genes were identified as likely players in canine OSA, with mutations in genes like TP53 retrieved in the cell lines. The findings provide molecular characterization for potential therapeutic targets and biomarkers in canine OSA.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Plant Sciences
Karolina Kolarova, Martina Nespor Dadejova, Tomas Loja, Gabriela Lochmanova, Eva Sykorova, Martina Dvorackova
Summary: The disruption of the H2A-H2B histone chaperone NUCLEOSOME ASSEMBLY PROTEIN 1 (NAP1) can suppress the loss-of-function phenotype of FAS1, leading to wild-type growth, decreased sensitivity to genotoxic stress, and suppression of telomere and 45S rDNA loss in Arabidopsis thaliana. This study demonstrates the essential role of NAP1 proteins in DNA repair in the absence of functional CAF-1, which is coupled to nucleosome assembly through modulation of H3 levels in the nucleus.
Article
Genetics & Heredity
Jan Papez, Jiri Starha, Pavel Zerhau, Denisa Pavlovska, Marta Jezova, Tomas Jurencak, Katerina Slaba, Martin Sterba, Arpad Kerekes, Tomas Merta, Terezia Haluskova, Hana Palova, Ondrej Slaby, Jaroslav Sterba, Petr Jabandziev
Summary: Spindle cell hemangioma and reninoma are extremely rare tumors in children and adolescents. Clinical diagnosis of the tumors requires consideration of factors such as pain level, family history, and secondary hypertension.
Article
Hematology
Vaclav Seda, Eva Vojackova, Laura Ondrisova, Lenka Kostalova, Sonali Sharma, Tomas Loja, Gabriela Mladonicka Pavlasova, Daniel Zicha, Marie Kudlickova Peskova, Jan Krivanek, Kvetoslava Liskova, Leos Kren, Vladimir Benes, Katerina Musilova Litzmanova, Marek Borsky, Jan Oppelt, Jan Verner, Sarka Pospisilova, Yvona Brychtova, Anna Panovska, Zhi Tan, Shuxing Zhang, Michael Doubek, Katerina Amruz Cerna, Jiri Mayer, Marek Mraz
Summary: The migration and survival of CLL cells are influenced by GAB1, which can be targeted for therapeutic purposes, especially in combination with BTK inhibitors.
Article
Oncology
Peter Mudry, Michal Kyr, Ondrej Rohleder, Michal Mahdal, Iva Staniczkova Zambo, Marta Jezova, Tomas Tomas, Jaroslav Sterba
Summary: The study reported potential benefits of mifamurtide in conventional therapy and compared it to a historical control group. The results showed that adding mifamurtide had a significant progression-free survival benefit for localized osteosarcoma patients.
JOURNAL OF BONE ONCOLOGY
(2021)
Article
Public, Environmental & Occupational Health
Denisa Krejci, Matej Karolyi, Lucie Pehalova, Jakub Scavnicky, Michaela Zapletalova, Ivana Katinova, Jaroslav Sterba, Jan Stary, Lenka Snajdrova, Martin Komenda, Ladislav Dusek
Summary: The study developed a new web portal called the Czech Childhood Cancer Information System to provide information on childhood cancer epidemiology in the country. By validating data from multiple sources, the portal offers basic information on childhood cancer including incidence, mortality, and overall survival rates through interactive reports.
JMIR PUBLIC HEALTH AND SURVEILLANCE
(2021)
Article
Engineering, Biomedical
Hana Wiedermannova, Peter Mudry, Jan Pavlicek, Hana Tomaskova, Andrea Hladikova, Hana Palova, Petra Vesela, Ondrej Slaby, Jaroslav Sterba
Summary: This study aimed to determine the incidence and risk factors of neoplastic diseases in the early stages of life. The results identified medications used by the mother before or during pregnancy and genetic diseases as the highest risk factors for early childhood tumors.
BIOMEDICAL PAPERS-OLOMOUC
(2023)
Review
Oncology
Andrew D. J. Pearson, Claudia Rossig, Crystal Mackall, Nirali N. Shah, Andre Baruchel, Gregory Reaman, Rosanna Ricafort, Delphine Heenen, Abraham Bassan, Michael Berntgen, Nick Bird, Eric Bleickardt, Najat Bouchkouj, Peter Bross, Carrie Brownstein, Sarah Beaussant Cohen, Teresa de Rojas, Lori Ehrlich, Elizabeth Fox, Stephen Gottschalk, Linda Hanssens, Douglas S. Hawkins, Ivan D. Horak, Danielle H. Taylor, Courtney Johnson, Dominik Karres, Franca Ligas, Donna Ludwinski, Maksim Mamonkin, Lynley Marshall, Behzad K. Masouleh, Yousif Matloub, Shannon Maude, Joe McDonough, Veronique Minard-Colin, Koen Norga, Karsten Nysom, Alberto Pappo, Laura Pearce, Rob Pieters, Martin Pule, Alfonso Quintas-Cardama, Nick Richardson, Martina Schuessler-Lenz, Nicole Scobie, Martina A. Sersch, Malcolm A. Smith, Jaroslav Sterba, Sarah K. Tasian, Brenda Weigel, Susan L. Weiner, Christian Michel Zwaan, Giovanni Lesa, Gilles Vassal
Summary: The seventh multi-stakeholder Paediatric Strategy Forum discussed the use of chimeric antigen receptor (CAR) T-cells in pediatric cancer treatment. The development of CAR T-cells for patients with haematological malignancies, especially B-cell precursor acute lymphoblastic leukaemia (BCP-ALL), has been successful. However, there are challenges in using CAR T-cells for other pediatric malignancies such as acute myeloid leukaemia (AML), lymphomas, and solid tumors. The forum aimed to summarize the current landscape, identify challenges and future directions, and determine the best strategies to accelerate the development and availability of CAR T-cell therapy for children. The forum emphasized the importance of improving efficacy, exploring alternative targets, and engaging academia and industry to advance cellular immunotherapies in pediatric oncology.
EUROPEAN JOURNAL OF CANCER
(2022)
Article
Biochemistry & Molecular Biology
Peter Macsek, Jan Skoda, Maria Krchniakova, Jakub Neradil, Renata Veselska
Summary: Novel thiosemicarbazones show promise in targeting MYCN-amplified and non-amplified neuroblastoma by reducing N-MYC and c-MYC expression, and decreasing total EGFR levels and phosphorylation through the involvement of NDRG1, a positive prognostic marker in neuroblastoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Johannes E. Wolff, Stefaan W. Van Gool, Tezer Kutluk, Blanca Diez, Rejin Kebudi, Beate Timmermann, Miklos Garami, Jaroslav Sterba, Gregory N. Fuller, Brigitte Bison, Uwe R. Kordes
Summary: Standards for chemotherapy against choroid plexus tumors have not yet been established. In this study, Carboplatin/Etoposide/Vincristine (CarbEV) was found to be more effective than Cyclophosphamide/Etoposide/Vincristine (CycEV). Histological grading was the most influential prognostic factor.
JOURNAL OF NEURO-ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Silvia Paukovcekova, Maria Krchniakova, Petr Chlapek, Jakub Neradil, Jan Skoda, Renata Veselska
Summary: This study identified a new mechanism for overcoming multidrug resistance in anticancer therapy. By combining anthracyclines with thiosemicarbazones, synergistic effects were observed in cell types derived from pediatric solid tumors. The synergism was independent of Pgp expression and resulted from impaired DNA repair control leading to cell death. The concomitant application of these drugs achieved the strongest synergistic effect with lower concentrations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Maria Krchniakova, Silvia Paukovcekova, Petr Chlapek, Jakub Neradil, Jan Skoda, Renata Veselska
Summary: This study analyzed the effects of combining three TKIs and two iron-chelating agents in the treatment of pediatric solid tumors. The results showed that the combination of these drugs had synergistic and/or additive effects, inhibiting cell signaling and inducing apoptosis. The combination of lapatinib with the iron-chelating agents had the most significant effects. These findings provide a rationale for the novel strategy of combining iron-chelating agents with TKIs in the therapy of pediatric solid tumors.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Oncology
Dominik Karres, Giovanni Lesa, Franca Ligas, Sylvie Benchetrit, Sara Galluzzo, Karen Van Malderen, Jaroslav Sterba, Maaike van Dartel, Marleen Renard, Peter Sisovsky, Siri Wang, Koen Norga
Summary: Regulatory decisions on paediatric investigation plans (PIPs) are crucial for providing effective and safe medicines for children with unmet medical needs, particularly in the field of paediatric oncology. Coordinating with stakeholders is essential for prioritizing and accelerating drug development in this challenging area.
EUROPEAN JOURNAL OF CANCER
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
L. Curylova, J. Neradil, M. Kyr, R. Veselska, J. Skoda
Article
Oncology
Michal Rihacek, Iveta Selingerova, Ivo Kocak, Ilona Kocakova, Eva Rihackova, Dalibor Valik, Jaroslav Sterba
Summary: This study retrospectively analyzed the data of adult and pediatric patients treated with sunitinib, and found that sunitinib treatment increased the mean corpuscular volume of erythrocytes. The study also found that the rate of increase was faster in patients who experienced treatment-related adverse events.
Article
Oncology
Petra Pokorna, Giannoula Lakka Klement, Alzbeta Vasikova, Veronika Kanderova, Marta Jezova, Kristyna Noskova, Peter Mudry, Michal Kyr, Tomas Merta, Viera Bajciova, Zdenka Krenova, Hana Palova, Dalibor Valik, Lenka Zdrazilova Dubska, Ondrej Slaby, Jaroslav Sterba
JCO PRECISION ONCOLOGY
(2022)