Experimental design approach in recombinant protein expression: determining medium composition and induction conditions for expression of pneumolysin from Streptococcus pneumoniae in Escherichia coli and preliminary purification process

Optimization of medium formulation and seed conditions for expression of mature PsaA (pneumococcal surface adhesin A) in Escherichia coli using a sequential experimental design strategy and response surface methodology

(2012) Ariane Leites Larentis et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Cloning and optimization of induction conditions for mature PsaA (pneumococcal surface adhesin A) expression in Escherichia coli and recombinant protein stability during long-term storage

(2011) Ariane Leites Larentis et al.   PROTEIN EXPRESSION AND PURIFICATION

Cloning and expression of protease ClpP from Streptococcus pneumoniae in Escherichia coli: Study of the influence of kanamycin and IPTG concentration on cell growth, recombinant protein production and plasmid stability

(2011) Karen Einsfeldt et al.   VACCINE

Influence of induction conditions on the expression of carbazole dioxygenase components (CarAa, CarAc, and CarAd) from Pseudomonas stutzeri in recombinant Escherichia coli using experimental design

(2010) Ariane Leites Larentis et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Novel mucosal vaccines generated by genetic conjugation of heterologous proteins to pneumolysin (PLY) from Streptococcus pneumoniae

(2010) Gill Douce et al.   VACCINE

Efficient experimental design and micro-scale medium enhancement of 6-deoxyerythronolide B production through Escherichia coli

(2009) Michael Pistorino et al.   BIOTECHNOLOGY PROGRESS

Pneumolysin: A Double-Edged Sword During the Host-Pathogen Interaction

(2008) Helen Marriott et al.   CURRENT MOLECULAR MEDICINE

Statistical optimization of medium for the production of pyruvate oxidase by the recombinant Escherichia coli

(2008) Jie Zhao et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

The role of Streptococcus pneumoniae virulence factors in host respiratory colonization and disease

(2008) Aras Kadioglu et al.   NATURE REVIEWS MICROBIOLOGY

Optimization of glutaryl-7-aminocephalosporanic acid acylase expression in E. coli

(2008) Federica Volontè et al.   PROTEIN EXPRESSION AND PURIFICATION

High-level expression of soluble viral structural protein in Escherichia coli

(2007) Yap P. Chuan et al.   JOURNAL OF BIOTECHNOLOGY