Article
Chemistry, Multidisciplinary
Dusan Kolarski, Simon Miller, Tsuyoshi Oshima, Yoshiko Nagai, Yugo Aoki, Piermichele Kobauri, Ashutosh Srivastava, Akiko Sugiyama, Kazuma Amaike, Ayato Sato, Florence Tama, Wiktor Szymanski, Ben L. Feringa, Kenichiro Itami, Tsuyoshi Hirota
Summary: CRY1 and CRY2 proteins are essential components of the circadian clock. Developing CRY1-selective compounds that can be manipulated through light-dependent mechanisms allows for a better understanding of the specific functions of CRY1 and CRY2. By utilizing benzophenone derivatives to interact with the photo lyase region of CRY1, researchers were able to activate CRY1 without affecting CRY2, demonstrating a potential for spatiotemporal regulation of CRY1 activity for health and disease research.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Plant Sciences
Hayato Yoshioka, Keiko Kimura, Yuko Ogo, Namie Ohtsuki, Ayako Nishizawa-Yokoi, Hironori Itoh, Seiichi Toki, Takeshi Izawa
Summary: Flowering in plants is an important biological process that determines the timing of reproduction based on photoperiod. Genes like Ghd7 and Hd1 in rice play different roles under long and short day lengths, and real-time monitoring of their protein products is required to understand the accurate photoperiod recognition at a molecular level.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Genetics & Heredity
Yaling Wu, Haijiao Zhao, Eric Erquan Zhang, Na Liu
Summary: PCBP1 is a novel clock modifier that negatively regulates the activation of the clock by enhancing the association of CRY1 with the CLOCK/BMAL1 complex.
FRONTIERS IN GENETICS
(2021)
Review
Cell Biology
Jonathan S. Yi, Nicolas M. Diaz, Shane D'Souza, Ethan D. Buhr
Summary: Most organisms have self-sustained circadian clocks that can be synchronized by environmental stimuli or oscillate indefinitely. In mammals, this is true at the molecular level for most cell types, with a core set of clock genes forming a transcriptional/translational feedback loop (TTFL) with a 24-hour period. The TTFL mechanism varies slightly in different cell types, but all involve similar core clock genes. The clock has unique outputs in different tissues, as cells convert the TTFL timing signals into orchestrated transcriptional oscillations of clock-controlled genes and cellular processes.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Xuemei Cao, Yanyan Yang, Christopher P. Selby, Zhenxing Liu, Aziz Sancar
Summary: The mammalian circadian clock is regulated by a transcription-translation feedback loop involving CLOCK-BMAL1 activators and CRY-PER repressors. Previous studies have shown that CRY and CRY-PER inhibit CLOCK-BMAL1 in different mechanisms. This study reveals the involvement of CRY-PER in displacing CLOCK-BMAL1 by recruiting CK1 delta, leading to the dissociation of CLOCK-BMAL1 from the E-box.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Mingxu Fang, Archana G. Chavan, Andy LiWang, Susan S. Golden
Summary: In this study, the researchers used the IVC reactions and massively parallel experiments to study entrainment, the synchronization of the circadian clock with the environment. The results showed that the IVC better explains the in vivo clock-resetting phenotypes of wild-type and mutant strains, and the output components are deeply engaged with the core oscillator, affecting the way input signals entrain the core pacemaker. These findings blur the line between input and output pathways and support the previous demonstration that key output components are fundamental parts of the clock.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Serena Riccitelli, Fabio Boi, Davide Lonardoni, Lidia Giantomasi, Olga Barca-Mayo, Davide De Pietri Tonelli, Silvia Bisti, Stefano Di Marco, Luca Berdondini
Summary: Clock genes in glial cells play an important role in regulating visual information processing in the retina, affecting retinal physiology and the activity of retinal ganglion cells in a circadian manner.
SCIENTIFIC REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Sha Sun, Han Wang
Summary: Epilepsy is a neurological disorder characterized by recurrent neuronal activities and seizures, with daily variations in seizure occurrence. Circadian misalignment and clock gene variants contribute to epileptic pathogenesis. This review discusses epilepsy-related genes, the circadian rhythmicity of epilepsies, and the mutual effects between epilepsy and sleep. It also highlights the advantages and challenges of animal models in epileptic studies. The review concludes with a recommendation for chronomodulated strategy-based chronotherapy for rhythmic epilepsies to develop time-specific antiepileptic drug dosing schedules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Amnon Brzezinski, Seema Rai, Adyasha Purohit, Seithikurippu R. Pandi-Perumal
Summary: Circadian rhythms play a crucial role in controlling reproductive function, with melatonin regulating the reproductive responses of animals to environmental light conditions. Clock genes and circadian rhythms are associated with optimal reproductive performance through the synchronization of endogenous molecular clocks in various tissues throughout the body.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Hsin-tzu Wang, Shiori Miyairi, Miho Kitamura, Kosuke Iizuka, Yoshimasa Asano, Takashi Yoshimura, Naohiro Kon
Summary: Real-time monitoring of cellular temperature responses is crucial in thermal biology and drug development. It has been discovered that Na+/Ca2+ exchanger (NCX)-dependent Ca2+ influx transduces cold signals to the circadian clock in mammalian cultured cells. By analyzing clock gene expression, cellular responses to cold signals can be evaluated. The study developed a luciferase-based real-time reporting system using Per1 promoter, Per2 promoter, Ca2+/cAMP-response elements (CRE), or NFAT-binding elements to monitor cold Ca2+-dependent transcription in living cells.
SCIENTIFIC REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Li-Ping Liu, Meng-Huan Li, Yun-Wen Zheng
Summary: Clock genes in hair follicles are regulated by both the central circadian system and external factors such as light and thyroid hormones. They play a role in regulating physiological processes of hair follicles, including hair growth and pigmentation. Hair follicles can serve as a noninvasive method for monitoring circadian-rhythm-related conditions in humans, and combining hair follicle sampling with other assays can improve their applications. Hair follicles provide insights into potential mechanisms of circadian-rhythm-related conditions and can support personalized treatment scheduling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Sara Moreno-SanJuan, Jose D. Puentes-Pardo, Jorge Casado, Julia Escudero-Feliu, Huda Khaldy, Javier Arnedo, Angel Carazo, Josefa Leon
Summary: The potential use of agomelatine as an alternative treatment for colorectal cancer is evaluated in this study. In vitro and in vivo models were used to study the effects of agomelatine on cell lines with different p53 statuses. Both agomelatine and melatonin showed inhibitory effects on the cells, with agomelatine being more effective. Agomelatine also regulated more clock genes and demonstrated a greater oncostatic effect in colorectal cancer.
Review
Biochemistry & Molecular Biology
Heather Ballance, Bokai Zhu
Summary: Biological oscillations often cycle at different harmonics of the 24-hour circadian rhythms, but the 12-hour oscillation is also evolutionarily conserved and gaining attention. The 12-hour rhythms are mainly regulated by XBP1s, generating cycles of gene expression.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Jia Hwei Cheong, Xiao Qiu, Yang Liu, Ahmad Al-Omari, James Griffith, Heinz-Bernd Schuttler, Leidong Mao, Jonathan Arnold
Summary: We investigated the phase synchronization of cellular clocks and found that the macroscopic limit is 150,000 cells or less. The maximum radius of a hypothesized quorum sensing signal molecule was estimated to be 13.05 nm using a diffusion approximation for signal travel. By using a second microwell device, we refined the macroscopic limit to a cell density of 2166 cells per fixed area for phase synchronization.
SCIENTIFIC REPORTS
(2022)
Article
Genetics & Heredity
Juan M. Jauregui-Lozano, Hana Hall, Sarah M. Stanhope, Kimaya Bakhle, Makayla M. Marlin, Vikki Weake
Summary: In this study, the authors used genomic and bioinformatic approaches to identify transcription factor binding sites with differential activity in aging Drosophila retina outer photoreceptors. They found Clock and Cycle to be key transcription factors involved in these age-regulated changes. Disruption of the Clock:Cycle complex resulted in light-dependent retinal degeneration and increased oxidative stress in Drosophila eyes. Their findings suggest that studying the Drosophila retina can provide insight into how disruption of circadian rhythms contributes to neurodegeneration in the retina.
Article
Cardiac & Cardiovascular Systems
Keiko Sumimoto, Hidekazu Tanaka, Jun Mukai, Kentaro Yamashita, Yusuke Tanaka, Ayu Shono, Makiko Suzuki, Shun Yokota, Makiko Suto, Hiroki Takada, Kensuke Matsumoto, Yu Taniguchi, Noriaki Emoto, Ken-ichi Hirata
INTERNATIONAL JOURNAL OF CARDIOVASCULAR IMAGING
(2020)
Article
Multidisciplinary Sciences
Pranindya Rinastiti, Koji Ikeda, Elda Putri Rahardini, Kazuya Miyagawa, Naoki Tamada, Yuko Kuribayashi, Ken-ichi Hirata, Noriaki Emoto
Article
Multidisciplinary Sciences
Agian Jeffilano Barinda, Koji Ikeda, Dhite Bayu Nugroho, Donytra Arby Wardhana, Naoto Sasaki, Sakiko Honda, Ryota Urata, Satoaki Matoba, Ken-ichi Hirata, Noriaki Emoto
NATURE COMMUNICATIONS
(2020)
Article
Pharmacology & Pharmacy
Gusty Rizky Teguh Ryanto, Kennosuke Yorifuji, Koji Ikeda, Noriaki Emoto
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
(2020)
Article
Multidisciplinary Sciences
Gusty R. T. Ryanto, Koji Ikeda, Kazuya Miyagawa, Ly Tu, Christophe Guignabert, Marc Humbert, Tomoyuki Fujiyama, Masashi Yanagisawa, Ken-ichi Hirata, Noriaki Emoto
Summary: The dysregulation of the activin A-bone morphogenetic protein receptor type 2 link in endothelium plays a critical role in the progression of pulmonary arterial hypertension. Inhibin-beta -A/activin-A in endothelial cells could be a potential pharmacotherapeutic target for the treatment of pulmonary arterial hypertension.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Adam Prabata, Koji Ikeda, Elda Putri Rahardini, Ken-Ichi Hirata, Noriaki Emoto
Summary: The study demonstrates that GPNMB negatively regulates macrophage inflammatory capacities and ameliorates WAT inflammation in obesity, making it a promising therapeutic target for metabolic disorders associated with obesity.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Biology
Andreas Haryono, Risa Ramadhiani, Gusty Rizky Teguh Ryanto, Noriaki Emoto
Summary: In this review, the basic functions of endothelin and related molecules, including their receptors and enzymes, are described. The important role of endothelin in cardiovascular diseases is discussed, along with the clinical evidence for targeting the endothelin pathway and the future scope of endothelin-targeting treatments. The current uses of endothelin receptor antagonists and advancements in treatment options are highlighted, providing an overview of endothelin research over the years and its potential future developments.
Article
Pharmacology & Pharmacy
Ratih Paramita Suprapto, Yoko Suzuki, Tatsuya Nagano, Ken-Ichi Hirata, Noriaki Emoto
Summary: Lung adenocarcinoma, the leading cause of cancer-related deaths worldwide, was found to have increased expression of endothelin-2 (ET-2) which was associated with poor overall survival. Silencing ET-2 inhibited proliferation, migration, and invasion, and enhanced apoptosis in lung adenocarcinoma cells.
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
(2022)
Article
Biology
Keisuke Miwa, Yu Taniguchi, Hiroyuki Fujii, Yoichiro Matsuoka, Hiroyuki Onishi, Kenichi Yanaka, Yu Izawa, Yasunori Tsuboi, Atsushi Kono, Noriaki Emoto, Kenichi Hirata
Summary: Poor subpleural perfusion (PSP) on dual-energy computed tomography (DE-CT) is a specific imaging finding in chronic thromboembolic pulmonary hypertension (CTEPH) and suggests a different mechanism of microvasculopathy from that in pulmonary arterial hypertension (PAH).
Article
Neurosciences
Shoji Kawauchi, Taiji Mizoguchi, Sayo Horibe, Toru Tanaka, Naoto Sasaki, Koji Ikeda, Noriaki Emoto, Ken-ichi Hirata, Yoshiyuki Rikitake
Summary: This study investigated the effects of aging-related changes on the neurovascular unit (NVU). It was found that senescence in the vascular endothelial cells led to morphological alterations in astrocytes and affected the localization of the water channel AQP4 at the gliovascular interface.
Article
Cardiac & Cardiovascular Systems
Hiroyuki Fujii, Yu Taniguchi, Sachiyo Yoneda, Keisuke Miwa, Yoichiro Matsuoka, Kenichi Yanaka, Yasunori Tsuboi, Noriaki Emoto, Kenichi Hirata
Summary: In this study, data from 149 patients with chronic thromboembolic pulmonary hypertension (CTEPH) were analyzed. The results showed that the efficacy and safety of balloon pulmonary angioplasty (BPA) for CTEPH with concurrent chronic obstructive pulmonary disease (COPD) were similar to those without COPD. Oxygenation and forced expiratory volume in 1 second/forced vital capacity showed improvement in patients with COPD. Therefore, BPA should be considered as a treatment option for CTEPH patients with concurrent COPD.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)
Review
Biochemistry & Molecular Biology
Gusty Rizky Teguh Ryanto, Ahmad Musthafa, Tetsuya Hara, Noriaki Emoto
Summary: Advances in technology and biomedical knowledge have improved the diagnosis and treatment of rare diseases, including pulmonary arterial hypertension (PAH). This article explores the role of activins and inhibins in PAH development and their connection to signaling pathways implicated in the pathogenesis of PAH. It also discusses the potential therapeutic benefits of activin/inhibin-targeting drugs, such as sotatercep, in improving patient outcomes. Overall, this article highlights the importance of activin/inhibin signaling as a critical mediator in PAH development and its potential as a target for future treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Sayo Horibe, Takuo Emoto, Taiji Mizoguchi, Toru Tanaka, Shoji Kawauchi, Naoto Sasaki, Tomoya Yamashita, Koji Ikeda, Noriaki Emoto, Ken-ichi Hirata, Yoshiyuki Rikitake
Summary: Alzheimer's disease is a common neurodegenerative disease associated with aging. Endothelial cell senescence has been found in the brains of Alzheimer's disease patients, but its role in the pathogenesis of the disease is unclear. The study revealed that endothelial cell senescence alters microglial morphology and function, providing a potential clue for the prevention and treatment of Alzheimer's disease.
Article
Cardiac & Cardiovascular Systems
Muhammad R. Hadwiono, Anggoro B. Hartopo, Widya Wasityastuti, Dyah W. Anggrahini, Gusty R. T. Ryanto, Noriaki Emoto, Lucia K. Dinarti
Summary: This study found that Activin A may have a role in the development and progression of pulmonary artery hypertension (PAH) caused by uncorrected congenital heart disease (CHD-PAH). Serum levels of Activin A were significantly increased in subjects with uncorrected CHD, and were even more significantly elevated in CHD-PAH patients compared to controls. Therefore, the measurement of serum Activin A shows promise in identifying uncorrected CHD patients with PAH development and progression.
PULMONARY CIRCULATION
(2023)
Article
Rehabilitation
Annis Rakhmawati, Indera Noor Achmad, Anggoro Budi Hartopo, Dyah Wulan Anggrahini, Irsad Andi Arso, Noriaki Emoto, Lucia Kris Dinarti
ANNALS OF REHABILITATION MEDICINE-ARM
(2020)