Article
Multidisciplinary Sciences
Shovon Lal Sarkar, A. S. M. Rubayet Ul Alam, Prosanto Kumar Das, Md Hasan Ali Pramanik, Hassan M. Al-Emran, Iqbal Kabir Jahid, M. Anwar Hossain
Summary: A simple and cost-effective alternative diagnostic method for COVID-19 was developed based on melting curve analysis. Compared to commercial kits, this method shows high specificity and sensitivity, with lower cost.
SCIENTIFIC REPORTS
(2022)
Article
Food Science & Technology
Ane del Rio-Lavin, Elisa Jimenez, Miguel Angel Pardo
Summary: The study developed a real-time PCR assay for the identification of Mytilus spp. mussels in the European market, showing 100% specificity, sensitivity, and precision, and can be easily applied across different platforms without special software requirements.
Article
Multidisciplinary Sciences
D. R. Marinowic, G. Zanirati, F. V. F. Rodrigues, M. V. C. Grahl, A. M. Alcara, D. C. Machado, J. C. Da Costa
Summary: A low-cost, highly specific method for SARS-CoV-2 detection has been successfully developed using the SYBR Green RT-PCR method, with the ability to screen out negative cases and high safety and efficacy. Optimization of experimental parameters achieved high specificity of PCR products, providing technical support for large-scale screening.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Eiseul Kim, Dayoung Kim, Seung-Min Yang, Hae-Yeong Kim
Summary: In this study, a multiplex real-time PCR method was developed to identify and differentiate three closely related species in the Lactobacillus acidophilus group. The method showed high specificity and sensitivity, with a detection limit of 10(1) CFU/ml. It can be used for monitoring these species in probiotic and dairy products.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Chemistry, Applied
Wenjie Xu, Mingyang Fu, Manhong Huang, Xiaowen Cui, Yi Li, Min Cao, Libin Wang, Xiaohui Xiong, Xiong Xiong
Summary: A novel method for rapid identification of S. solar and O. mykiss based on duplex PCR assay was developed successfully in this study. Specific primers design and melting curve analysis enabled accurate discrimination of the two fish species, offering a feasible solution for detecting adulteration in processed fish products.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Food Science & Technology
Jinchun Li, Jiapeng Li, Ruixi Liu, Yixuan Wei, Shouwei Wang
Summary: The study developed a hexaplex real-time PCR assay using melting curve analysis to identify eleven meat species simultaneously, showing good specificity of primers and detection limits of 0.01-0.1 ng DNA and 0.1-1% meat mixture. The method can accurately identify different meat species, indicating great application potential.
Article
Medicine, General & Internal
Ayesha Nayyar, Suhaib Ahmed
Summary: The study aimed to optimize and evaluate real time PCR of Single Nucleotide Polymorphism by SYBR Green for detecting donor chimerism after haematopoietic stem cell transplantation. The results showed that this method was sensitive and effective in detecting chimerism in post-transplant patients with various haematological disorders.
PAKISTAN JOURNAL OF MEDICAL SCIENCES
(2021)
Article
Multidisciplinary Sciences
Qiuying Huang, Dongmei Chen, Chen Du, Qiaoqiao Liu, Su Lin, Lanlan Liang, Ye Xu, Yiqun Liao, Qingge Li
Summary: The article introduces a method called MeltArray, which allows for the detection of multiple targets in a single real-time PCR reaction. This method utilizes the enzyme activity of Taq DNA polymerase to generate fluorescent hybrids with different melting temperatures, enabling the detection of multiple targets. MeltArray has been validated with clinical samples and demonstrates the advantages of multiplicity, versatility, simplicity, and accessibility.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Applied
Jiapeng Li, Yixuan Wei, Jinchun Li, Ruixi Liu, Suigen Xu, Suyue Xiong, Ya Guo, Xiaoling Qiao, Shouwei Wang
Summary: An efficient and reliable duplex SYBR Green real-time quantitative PCR method was developed for detecting beef adulteration, utilizing bovine specific and vertebrate universal primers to identify both bovine and non-bovine components. The method also semi-quantified the percentage of bovine DNA present in samples. The limit of detection was 1 pg for bovine DNA and varied for non-bovine DNA depending on the species.
Article
Biochemical Research Methods
Andreas Untergasser, Jan M. Ruijter, Vladimir Benes, Maurice J. B. van den Hoff
Summary: The analysis of amplification and melting curves in qPCR experiments provides valuable information on reaction quality and leads to reliable results. The LinRegPCR web application allows visualization and analysis of individual qPCR runs, highlighting deviant reactions for further calculation of gene-expression ratios and fold-change between conditions.
BMC BIOINFORMATICS
(2021)
Article
Biochemical Research Methods
Yong Wang, Ziteng Fu, Xu Guo, Da Zhang, Caixia Bai, Wei Li, Guangqing Liu, Yongdong Li, Shudong Jiang
Summary: A SYBR Green I-based real-time RT-PCR method was developed for clinical diagnosis of feline astroviruses, showing good specificity, sensitivity, and reproducibility. The method has a higher positive detection rate compared to conventional RT-PCR and is cheap and effective for large-scale clinical testing and epidemiological investigation.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Chemistry, Applied
Jinchun Li, Jiapeng Li, Yixuan Wei, Suigen Xu, Sueyue Xiong, Dan Li, Shouwei Wang, Anqi Liang
Summary: Meat adulteration is a significant issue in the meat supply chain, and it can seriously harm consumers' economic and health interests. To address the problem of mutton adulteration and reduce testing costs, a triplex real-time PCR method with melting curve analysis was developed to identify three meat categories simultaneously.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Biochemical Research Methods
Liting Sun, Zhiqing Xu, Junhuang Wu, Yongqiu Cui, Xu Guo, Fazhi Xu, Yongdong Li, Yong Wang
Summary: A novel duplex SYBR Green I-based qPCR assay was developed for the rapid and simultaneous detection of FPV and FCoV, demonstrating sensitivity and specificity in detecting these viruses.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Biochemical Research Methods
Yin-he Xia, Zi-cong Shi, Xin-wei Wang, Yong-tao Li, Zeng Wang, Hong-tao Chang, Hong-ying Liu, Lu Chen, Chuan-qing Wang, Xia Yang
Summary: GETV, a mosquito-borne virus, is increasingly posing a threat to animal safety and public health. The newly developed RT-qPCR assay for GETV has high sensitivity and reproducibility, making it suitable for rapid diagnosis and quantitative analysis of GETV infection.
MOLECULAR AND CELLULAR PROBES
(2021)
Article
Genetics & Heredity
Jitendra K. Biswal, Biswa Ranjan Jena, Syed Zeeshan Ali, Rajeev Ranjan, Jajati K. Mohapatra, Rabindra Prasad Singh
Summary: A rapid, sensitive and reliable laboratory detection method for foot-and-mouth disease virus infection was developed in India. The new method showed higher sensitivity, reproducibility and specificity compared to traditional methods. It had a higher success rate in detecting positive samples compared to the traditional method.
Article
Biotechnology & Applied Microbiology
Shao-Hua Wang, Chih-Yu Huang, Chun-Yen Chen, Chia-Che Chang, Chun-Yung Huang, Cheng-Di Dong, Jo-Shu Chang
Summary: Fucoidans, sulfated polysaccharides of marine origin, were extracted from brown macroalgae using microwave-assisted and ultrasound-assisted extraction methods. The purified fucoidan product showed nearly 84.0% purity and exhibited anti-lipogenesis activity with a 28.9% decrease in lipid synthesis compared to the control.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Correction
Biochemistry & Molecular Biology
Fu Ou-Yang, I-Hsuan Tsai, Jen-Yang Tang, Ching-Yu Yen, Yuan-Bin Cheng, Ammad Ahmad Farooqi, Shu-Rong Chen, Szu-Yin Yu, Jun-Kai Kao, Hsueh-Wei Chang
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Yu-Chi Lin, Yi-Jen Chen, Shu-Rong Chen, Wan-Ju Lien, Hsueh-Wei Chang, Yu-Liang Yang, Chia-Ching Liaw, Jui-Hsin Su, Ching-Yeu Chen, Yuan-Bin Cheng
Summary: The application of LC-MS/MS-based molecular networking revealed that the ethanol extract of octocoral Asterospicularia laurae may serve as a potential source for the discovery of new xenicane derivatives. As a result of the natural product investigation, four new xenicane diterpenoids were identified, along with six known compounds. Among these compounds, asterolaurins O and R showed selective cytotoxic effects against the MCF-7 cell line.
Article
Biotechnology & Applied Microbiology
Chun-Yen Chen, Shao-Hua Wang, Chih-Yu Huang, Cheng-Di Dong, Chun-Yung Huang, Chia-Che Chang, Jo-Shu Chang
Summary: This study found that the decrease in molecular mass of fucoidan extracted from brown seaweed can enhance its antioxidant activity, anti-lipogenesis, and anti-inflammatory activities. Additionally, the degree of sulfation of fucoidan also affects its antioxidant and anti-inflammatory capabilities.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Environmental Sciences
Yun-Wei Chiang, Sheng-Wen Wu, Ci-Wen Luo, Shih-Pin Chen, Chun-Jung Chen, Wen-Ying Chen, Chia-Che Chang, Chuan-Mu Chen, Yu-Hsiang Kuan
Summary: The study found that exposure to PM2.5 increased the risk of glaucoma in patients with diabetes mellitus, demonstrating that PM2.5 is an independent risk factor for glaucoma in these patients.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Article
Environmental Sciences
Yun-Wei Chiang, Chun-Hung Su, Han-Yin Sun, Shih-Pin Chen, Chun-Jung Chen, Wen-Ying Chen, Chia-Che Chang, Chuan-Mu Chen, Yu-Hsiang Kuan
Summary: BPA, an estrogen-like compound, can enter the human body, affecting ARPE-19 cells and leading to cytotoxicity.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Engineering, Chemical
En-Ting Lin, Yi-Chieh Lee, Hui-Min David Wang, Chen-Yaw Chiu, Yu-Kaung Chang, Chun-Yung Huang, Chia-Che Chang, Pei-Chien Tsai, Jo-Shu Chang
Summary: Fucoidan is a polysaccharide with excellent antioxidant, anti-inflammatory, anti-angiogenic, antitumor, and antibacterial capabilities. Subcritical water extraction is an effective method for extracting fucoidan and preserving its bioactivity.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2022)
Article
Biotechnology & Applied Microbiology
Heyu Yang, Hui Wang, Chenghao Wen, Shun Bai, Pengfei Wei, Bo Xu, Yunjun Xu, Chaozhao Liang, Yunjiao Zhang, Guilong Zhang, Huiqin Wen, Li Zhang
Summary: This study aimed to evaluate the effects of iron oxide nanoparticles (IONPs) on the male reproductive system of mice. The results showed that IONPs did not affect the major organs, but caused damage to Sertoli cells in the blood-testis barrier (BTB) at a relatively high concentration. This led to a temporary reduction in sperm quantity and quality, which was attributed to increased oxidative stress and apoptotic activity in the epididymis. However, these effects were reversible and returned to normal within 14 days after injection of IONPs.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Min-Yung Kuo, Wei-Ting Yang, Yann-Jen Ho, Ge-Man Chang, Hsiung-Hao Chang, Chao-Yu Hsu, Chia-Che Chang, Yi-Hsin Chen
Summary: Bladder cancer is a common malignancy worldwide. STAT3 is a transcription factor commonly hyperactivated in cancers, including bladder cancer. HME, a compound derived from a medicinal mushroom, was found to be cytotoxic to bladder cancer cells by blocking STAT3 activation and suppressing the downstream survivin signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Hui Wang, Yu Guan, Chun Li, Jia Chen, Shaoyu Yue, Jieying Qian, Bangshun Dai, Changqin Jiang, Chenghao Wen, Longping Wen, Chaozhao Liang, Yunjiao Zhang, Li Zhang
Summary: This study designed magnetic PEGylated manganese-zinc ferrite nanocrystals (PMZFNs) to actively attract and retain the drug in prostate cancer by implanting micromagnets into tumor tissues. The PMZFNs accumulate in prostate cancer and elicit potent ferroptosis and the activation of cGAS-STING pathway, achieving targeted therapy.
Article
Biochemistry & Molecular Biology
Chao-Yu Hsu, Wei-Ting Yang, Ju-Hwa Lin, Chien-Hsing Lu, Kai-Cheng Hu, Tsuo-Hung Lan, Chia-Che Chang
Summary: Sertindole has been found to have antiproliferative effects on bladder cancer cells by inhibiting the STAT3/BCL-xL signaling pathway, suggesting its potential as a therapeutic strategy for bladder cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Chih-Pin Liao, Ya-Chu Hsieh, Chien-Hsing Lu, Wen-Chi Dai, Wei-Ting Yang, Kur-Ta Cheng, Modukuri V. Ramani, Gottumukkala V. Subbaraju, Chia-Che Chang
Summary: This study presents the first report on the anti-TNBC effect and action mechanism of Methoxyhispolon Methyl Ether (MHME). It was found that MHME induces apoptosis in TNBC cells by inhibiting the activation of STAT3 and downregulating BCL-2 expression. Additionally, MHME suppresses SRC activation. These findings suggest the potential of MHME as a TNBC chemotherapy agent.
Article
Biochemistry & Molecular Biology
Ya-Chu Hsieh, Yuan-Chang Dai, Kur-Ta Cheng, Wei-Ting Yang, Modukuri V. Ramani, Gottumukkala V. Subbaraju, Yi-Ju Chen, Chia-Che Chang
Summary: Colorectal cancer (CRC) is the third most prevalent human cancer globally, and current chemotherapy has limited effectiveness. This study found that Dehydroxyhispolon methyl ether (DHME) has a strong cytotoxic effect on CRC cells while being less toxic to normal colon epithelial cells. DHME induces CRC cell apoptosis by blocking the SRC/STAT3/BCL-2 axis, suggesting its potential as a CRC chemotherapy agent.
Article
Food Science & Technology
Chang-Ming Chern, Chung-Kuang Lu, Kuo-Tong Liou, Yea-Hwey Wang, Keng-Chang Tsai, Chia-Lin Chang, Chia-Che Chang, Yuh-Chiang Shen
Summary: This study demonstrates the neuroprotective effect of medicarpin on stroke-induced injury, reducing brain infarction, protecting the blood-brain barrier, and promoting brain repair through stimulation of neurogenesis and expression of neuroprotective proteins.
JOURNAL OF FOOD AND DRUG ANALYSIS
(2021)
Review
Biotechnology & Applied Microbiology
Yu-Chen Liao, Chia-Che Chang, Dillirani Nagarajan, Chun-Yen Chen, Jo-Shu Chang
Summary: Hydrocolloids are a type of food additives widely used in the food industry to develop structure in food ingredients. Algae-derived hydrocolloids such as carrageenan, agar, and alginate are commonly used as thickening, gelling, and emulsifying agents. Despite the debate surrounding carrageenan, algal hydrocolloids are considered a safe alternative to synthetic additives.