Article
Water Resources
Mohaddeseh Zahmatkesh Anbarani, Afsaneh Esmaeili Nasrabadi, Ziaeddin Bonyadi
Summary: The aim of this study was to evaluate the efficiency of S. cerevisiae in removing PS from aqueous solutions. BBD was used to determine the optimal removal conditions. The maximum PS removal efficiency was 98.81% under optimized conditions. Based on the results, it can be concluded that S. cerevisiae can be used as a natural and environmentally friendly biocoagulant to remove PS.
APPLIED WATER SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Mrinal Sharma, Prranshu Yadav, Ankita Doshi, Hemang D. Brahmbhatt, C. Ratna Prabha
Summary: The study examined the effects of six double mutants derived from the ubiquitin mutant UbEP42 on cell functions, finding that the double mutation L50P-I61T had the most detrimental impact. Overall, these double mutations made cells more sensitive, and the negative effects under certain conditions could be compensated for by each other.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Food Science & Technology
Rafaela Xavier Giacomini, Eliza Rodrigues Acosta, Maristela Barnes Rodrigues Cerqueira, Ednei Gilberto Primel, Jaqueline Garda-Buffon
Summary: This study investigated the reduction of pesticides 2,4-dichlorophenoxyacetic acid (2,4-D) and procymidone, as well as the mycotoxin ochratoxin A (OTA), through alcoholic fermentation and evaluated their effects on quality parameters of the process. The contaminated treatments showed decreased ethanol production and cell viability. After 168 hours of fermentation, the pesticides decreased by 22% and 65%, while OTA decreased by 52% and 58%. The decrease in procymidone and OTA was attributed to peroxidase activity and glutathione production in yeast cells.
FOOD AND BIOPROCESS TECHNOLOGY
(2023)
Article
Engineering, Environmental
Agnieszka Wegrzyn, Avtandil Tsurtsumia, Stefan Witkowski, Olga Freitas, Sonia Figueiredo, Joanna Cybinska, Wojciech Stawinski
Summary: This study found that the addition of vermiculite in yeast can reduce the toxicity of Cd2+ and enhance the adsorption capacity of yeast for Cd2+.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Biochemistry & Molecular Biology
Laura Vanderwaeren, Ruveyda Dok, Karin Voordeckers, Sandra Nuyts, Kevin J. Verstrepen
Summary: Yeast is an important enzyme source for bread and beer production and is an ideal model organism for studying eukaryotic cell biology and genetics. This review discusses the characteristics that make yeast such a widely used model organism, with a focus on the DNA damage response pathway as an example of its contribution to elucidating highly conserved biological processes. Differences in the DNA damage response of yeast and humans are also highlighted, along with the challenges of using yeast as a model system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Minami Ogawa, Jaime Moreno-Garcia, Lucy C. M. Joseph, Juan C. Mauricio, Juan Moreno, Teresa Garcia-Martinez
Summary: The study demonstrated that Saccharomyces cerevisiae flor yeast consumed about 30% of gluconic acid in artificial media, affecting several key metabolic pathways. This provides insights for future experiments to enhance the control of gluconic acid levels in fermented beverages production.
Article
Food Science & Technology
Jitka Laurent, Nore Struyf, An Bautil, Albina Bakeeva, Mikolaj Chmielarz, Marika Lyly, Beatriz Herrera-Malaver, Volkmar Passoth, Kevin J. Verstrepen, Christophe M. Courtin
Summary: A diet low in FODMAPs can help reduce symptoms in patients with irritable bowel syndrome, but avoiding cereal grain products may lead to nutritional deficiencies. Developing low-FODMAP, high-fiber cereal grain products using K. marxianus in bread production can effectively reduce fructan levels and maintain fructan and fructose levels below the threshold for low-FODMAP products.
FOOD AND BIOPROCESS TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lifang Yu, Mario Andrea Marchisio
Summary: This study presents an efficient tool to induce mRNA degradation in yeast using CRISPR-(d)Cas system, specifically (d)Cas12a. The study highlights the potential of V-A Cas proteins in yeast and demonstrates their applications in constructing Boolean logic gates.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Genetics & Heredity
Raquel Sorribes-Dauden, Maria Teresa Martinez-Pastor, Sergi Puig
Summary: Iron is essential for eukaryotic organisms, and its deficiency can lead to anemia. Yeast cells use the Ccc1 transporter to detoxify and store excess iron. Deletion of the Nt domain of Ccc1 can increase iron accumulation in cells.
Article
Immunology
Francesca Soutter, Dirk Werling, Matthew Nolan, Tatiana Kuster, Elizabeth Attree, Virginia Marugan-Hernandez, Sungwon Kim, Fiona M. Tomley, Damer P. Blake
Summary: A cheap and easy-to-produce oral vaccine has been developed using Saccharomyces cerevisiae yeast expressing Eimeria tenella antigens. The vaccine effectively reduces the replication of Eimeria tenella and improves the growth of chickens, making it an important tool for controlling coccidiosis in chickens, particularly in low- and middle-income countries.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Applied
Maria Agustina Caruso, Judith Araceli Piermaria, Analia Graciela Abraham, Micaela Medrano
Summary: Nowadays, there is a growing interest in finding new healthy compounds from by-products of the food industry. One such by-product is brewers spent yeast (BSY), which, if not properly treated, can pose an environmental problem. Certain components of BSY, particularly beta-glucans, have great potential as biological response modifiers for humans. This review summarizes the potential application of BSY by-product, specifically the extraction of cell wall components (beta-glucans), and focuses on their biological effects in the intestinal context.
FOOD HYDROCOLLOIDS
(2022)
Article
Biotechnology & Applied Microbiology
Carmen Berbegal, Sergi Ferrer, Lucia Polo, Isabel Pardo, Maria Jose Garcia-Esparza, Lorena Andres, Inmaculada Alvarez, Victoria Lizama
Summary: The aim of this study was to isolate and characterize indigenous Saccharomyces cerevisiae yeasts from Cabernet Sauvignon grape must and investigate their impact on the final characteristics of the wines produced. The study was conducted at a Pago winery in Requena, Spain. Twenty-two yeasts were isolated from the grape must, and after molecular identification and typing, 11 different strains were identified. Laboratory-scale fermentations were performed to determine growth-related parameters and metabolic characteristics, while larger-scale fermentations were used to analyze the chemical and sensory attributes of the wines produced. Differences between strains in terms of growth and metabolism were observed. The goal was to identify the strains that would produce the highest concentrations of polyphenols and aromatics in the red Cabernet wines of the winery for future use.
FERMENTATION-BASEL
(2023)
Article
Engineering, Environmental
Chao Yang, Zhuo Zhang, Bangzhu Peng
Summary: This study investigated the proteomic differences of Saccharomyces cerevisiae under PAT stress and identified proteins involved in redox reactions and defense mechanisms that are up-regulated to resist PAT stress. Molecular docking was then used to screen for a protein with the highest binding affinity to PAT, which was confirmed to be able to completely degrade PAT into another substance. This study provides new insights for the rapid screening and development of PAT degrading enzymes and offers a theoretical basis for the detoxification of mycotoxins.
JOURNAL OF HAZARDOUS MATERIALS
(2024)
Article
Chemistry, Applied
Hideaki Tokuyama, Ryo Aoyagi, Kazuto Fujita, Yuki Maekawa, Shohei Riya
Summary: Ethanol fermentation using a hybrid system containing free and immobilized yeast cells in macroporous hydrogel scaffold was studied, with immobilization achieved through NIPA and MTGA hydrogels. The hybrid system showed higher ethanol productivity than the control system, with macroporous hydrogel allowing stable growth of immobilized yeast cells. In steady-state fermentation, the hybrid system had significantly higher cell densities and ethanol productivities compared to the control system.
REACTIVE & FUNCTIONAL POLYMERS
(2021)
Article
Biotechnology & Applied Microbiology
Javier Garcia-Pardo, Aleksandra E. Badaczewska-Dawid, Carlos Pintado-Grima, Valentin Iglesias, Aleksander Kuriata, Sebastian Kmiecik, Salvador Ventura
Summary: This study presents the A3D yeast database (A3DyDB), which provides an extensive catalog of aggregation propensity predictions for the S. cerevisiae proteome, allowing for the evaluation of the influence of mutations on protein stability and solubility.
MICROBIAL CELL FACTORIES
(2023)
Article
Cell Biology
Norfadilah Hamdan, Paraskevi Kritsiligkou, Chris M. Grant
JOURNAL OF CELL BIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Paraskevi Kritsiligkou, Jonathan D. Rand, Alan J. Weids, Ximeng Wang, Chris J. Kershaw, Chris M. Grant
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Multidisciplinary Sciences
Nur Hidayah Jamar, Paraskevi Kritsiligkou, Chris M. Grant
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
David Talavera, Christopher J. Kershaw, Joseph L. Costello, Lydia M. Castelli, William Rowe, Paul F. G. Sims, Mark P. Ashe, Chris M. Grant, Graham D. Pavitt, Simon J. Hubbard
SCIENTIFIC REPORTS
(2018)
Article
Genetics & Heredity
Jing Li, Mark Rinnerthaler, Johannes Hartl, Manuela Weber, Thomas Karl, Hannelore Breitenbach-Koller, Michael Mulleder, Jakob Vowinckel, Hans Marx, Michael Sauer, Diethard Mattanovich, Ozge Ata, Sonakshi De, Gregor P. Greslehner, Florian Geltinger, Bill Burhans, Chris Grant, Victoria Doronina, Meryem Ralser, Maria Karolin Streubel, Christian Grabner, Stefanie Jarolim, Claudia Mosshammer, Campbell W. Gourlay, Jiri Hasek, Paul J. Cullen, Gianni Liti, Markus Ralser, Michael Breitenbach
G3-GENES GENOMES GENETICS
(2020)
Article
Biochemistry & Molecular Biology
Paraskevi Kritsiligkou, Karol Nowicki-Osuch, Zorana Carter, Chris J. Kershaw, Declan R. Creamer, Alan J. Weids, Chris M. Grant
Summary: The research discovers a direct protein interaction between Tsa1 and the PKA pathway in response to protein misfolding stress, leading to a targeted reprogramming of gene expression through oxidation-mediated regulation of PKA activity.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Correction
Multidisciplinary Sciences
Nur Hidayah Jamar, Paraskevi Kritsiligkou, Chris M. Grant
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Christopher J. Kershaw, Michael G. Nelson, Jennifer Lui, Christian P. Bates, Martin D. Jennings, Simon J. Hubbard, Mark P. Ashe, Chris M. Grant
Summary: This study systematically determined the protein and mRNA composition of P-bodies and stress granules before and after nutrient stress, revealing the existence of high molecular weight complexes as potential seeds for mature condensates. Shared proteins and RNA components between these biological condensates were identified, highlighting a complex interaction profile during their maturation process. The interaction networks represent a tunable response to stress, showcasing previously unrecognized condensate heterogeneity.
Review
Biochemistry & Molecular Biology
Declan R. Creamer, Simon J. Hubbard, Mark P. Ashe, Chris M. Grant
Summary: Eukaryotic cells have developed a complex circuitry of signalling molecules to monitor changes in their environments. The cAMP/PKA pathway is an important glucose sensing circuit in yeast, and PKA activity regulates various processes in yeast growth. However, the molecular basis of PKA signalling specificity is poorly understood.
Article
Biochemistry & Molecular Biology
Kwan Ting Kan, Michael G. Nelson, Chris M. Grant, Simon J. Hubbard, Hui Lu
Summary: Yme1 is a multifunctional protein that plays important roles in maintaining mitochondrial protein homeostasis and regulating biogenesis and function of mitochondrial proteins. This study shows that YME1 deletion affects yeast growth, chronological life span, mitochondrial protein homeostasis and function. It also reveals that Yme1 plays a key role in longevity and is important for maintaining the level and function of mitochondrial oxidative phosphorylation complexes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Martin D. Jennings, Priya Srivastava, Christopher J. Kershaw, David Talavera, Christopher M. Grant, Graham D. Pavitt
Summary: Cells respond to oxidative stress by reprogramming gene expression to enhance levels of antioxidant enzymes. In Saccharomyces cerevisiae, proteins Slf1 and Sro9 play a role in protein synthesis adaptation during stress. Slf1 is identified as a ribosome-associated translational modulator that promotes translation of highly-translated mRNAs, facilitating cell survival and adaptation to stress.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Jana Schepers, Zorana Carter, Paraskevi Kritsiligkou, Chris M. Grant
Summary: This study reveals that methionine oxidation is a key factor in the formation of the yeast [PSI+] prion. By lacking methionine sulfoxide reductases, it is found that [PSI+] formation is increased, while lacking both isoenzymes of methionine sulfoxide reductases reduces [PSI+] formation.
Article
Biochemistry & Molecular Biology
Joanne Cunningham, Aristeidis P. Sfakianos, Paraskevi Kritsiligkou, Christopher J. Kershaw, Alan J. Whitmarsh, Simon J. Hubbard, Mark P. Ashe, Chris M. Grant
Summary: Translation initiation factor 4G (eIF4G) is crucial for translation initiation. In yeast, eIF4G1 is specifically required for the translational response to oxidative stress.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Andrew F. Jarnuczak, Manuel Garcia Albornoz, Claire E. Eyers, Christopher M. Grant, Simon J. Hubbard
Article
Biotechnology & Applied Microbiology
Joseph L. Costello, Christopher J. Kershaw, Lydia M. Castelli, David Talavera, William Rowe, Paul F. G. Sims, Mark P. Ashe, Christopher M. Grant, Simon J. Hubbard, Graham D. Pavitt