Article
Chemistry, Physical
Silvia Anselmi, Alexandra T. P. Carvalho, Angela Serrano-Sanchez, Jose L. Ortega-Roldan, Jill Caswell, Iman Omar, Gustavo Perez-Ortiz, Sarah M. Barry, Thomas S. Moody, Daniele Castagnolo
Summary: This study identifies selective and robust MsrA biocatalysts capable of efficiently catalyzing enantioselective reduction of various aromatic and aliphatic chiral sulfoxides at concentrations of 8-64 mM with high yields and excellent enantiomeric excess (up to 99%). Through rational mutagenesis design using in silico docking, molecular dynamics, and structural nuclear magnetic resonance (NMR) studies, a mutant enzyme MsrA33 was developed, which can catalyze the kinetic resolution of bulky sulfoxide substrates with non-methyl substituents on the sulfur atom with enantiomeric excess up to 99%, overcoming a significant limitation of current MsrA biocatalysts.
Article
Biochemistry & Molecular Biology
Catherine Duport, Jean-Paul Madeira, Mahsa Farjad, Beatrice Alpha-Bazin, Jean Armengaud
Summary: The study reveals that under fermentative anoxic conditions, MsrA plays a significant role in antioxidant activities, affecting cell growth, motility, and energy metabolism.
Review
Chemistry, Multidisciplinary
Tao Peng, Xiaoling Cheng, Yongzheng Chen, Jiawei Yang
Summary: Chiral sulfoxides, valuable organosulfur compounds widely used in medicinal and organic synthesis, can be prepared through biocatalytic approaches, with sulfoxide reductases emerging as a new method with extraordinary catalytic properties. This review article discusses the chemical and biological functions of sulfoxide reductases and highlights their applications in chiral sulfoxide preparation.
FRONTIERS IN CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Tao Peng, Jin Tian, Yuyan Zhao, Xu Jiang, Xiaoling Cheng, Guozhong Deng, Quan Zhang, Zhongqiang Wang, Jiawei Yang, Yongzheng Chen
Summary: This study achieved the cyclic deracemization of sulfoxides by combining the high-enantioselective enzyme MsrA with the low-enantioselective enzyme SMO, resulting in enantiopure sulfoxides. The cofactors of the enzymes were regenerated by the cascade catalysis of auxiliary enzymes, enabling high yield and enantioselectivity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Biochemistry & Molecular Biology
Lionel Tarrago, Alaattin Kaya, Hwa-Young Kim, Bruno Manta, Byung-Cheon Lee, Vadim N. Gladyshev
Summary: Methionine sulfoxide reductases (MSR) are enzymes that reduce methionine sulfoxide (MetO) back to methionine. MSRB1, a selenoprotein found in mammals, uses selenocysteine as a catalytic residue. It plays a crucial role in oxidative stress protection, neuronal cell maintenance, cognition, cancer cell proliferation, and immune response.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Chemistry, Physical
Yan Zhao, Shuo Liu, Zhishang Shi, Hangqi Zhu, Mingchun Li, Qilin Yu
Summary: In this study, a pathogen infection-responsive and macrophage endoplasmic reticulum-targeting nanoplatform was developed to alleviate systemic infections. The nanoplatform efficiently loaded and released an antimicrobial peptide, inhibited pathogen growth and macrophage stress, and protected against kidney dysfunction and inflammation in a systemic infection model.
Article
Biochemistry & Molecular Biology
Hayat Hage, Marie-Noelle Rosso, Lionel Tarrago
Summary: Research shows that most fungi possess genes for three types of methionine sulfoxide reductase: MsrA, MsrB, and fRMsr in their genomes. However, some fungi living in anaerobic environments or as obligate intracellular parasites lack these genes. Additionally, several horizontal gene transfer events from bacteria to fungi involving msr genes have been identified.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Lihue N. Gonzalez, Matias S. Cabeza, Carlos Robello, Sergio A. Guerrero, Alberto A. Iglesias, Diego G. Arias
Summary: Trypanosoma cruzi is a unicellular parasite that causes Chagas Disease and infects various mammalian hosts. It exhibits auxotrophy for L-Met and requires the amino acid from the extracellular environment of the host. The study identified a coding sequence for a free-R-MSR enzyme in T. cruzi, which is involved in the reduction of L-MetSO using specific cysteine residues. The results provide new insights into the redox metabolism of T. cruzi and contribute to understanding L-Met metabolism in this parasite.
Article
Plant Sciences
Li Wang, Jieyu Yang, Wenjun Tan, Yile Guo, Jiaqi Li, Chuntao Duan, Gehong Wei, Minxia Chou
Summary: In the symbiosis of M. truncatula-Sinorhizobium meliloti, MtMIF3 is mainly expressed in the nitrogen-fixing zone of the nodules. Silencing MtMIF3 increased nodule numbers but resulted in higher bacteroid degradation in the infected cells of the nitrogen-fixing zone, indicating premature aging. On the other hand, overexpression of MtMIF3 inhibited nodule senescence.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Jana Schepers, Zorana Carter, Paraskevi Kritsiligkou, Chris M. Grant
Summary: This study reveals that methionine oxidation is a key factor in the formation of the yeast [PSI+] prion. By lacking methionine sulfoxide reductases, it is found that [PSI+] formation is increased, while lacking both isoenzymes of methionine sulfoxide reductases reduces [PSI+] formation.
Article
Biochemistry & Molecular Biology
Akira Hosomi, Chinatsu Okachi, Yudai Fujiwara
Summary: Soluble proteins with a signal peptide are translocated into the endoplasmic reticulum (ER), while some proteins without a signal peptide, like SOD1, can still enter the ER. SOD1 is a causative gene of ALS, and its secretion has been implicated in the pathogenesis of ALS. This study reveals that SOD1 is translocated into the ER lumen through the translocon Sec61 and subsequently secreted extracellularly, suggesting the potential of targeting SOD1 secretion as a therapeutic approach for ALS.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Haiyan Yang, Zhi Li, Zhangwei Wang, Xu Zhang, Xinyuan Dai, Guoren Zhou, Qiang Ding
Summary: The study found that HM13 is highly expressed in breast cancer and is associated with poor prognosis. Downregulation of HM13 can suppress the proliferation and metastasis abilities of breast cancer cells, activate autophagy, and inhibit the PI3K-AKT-mTOR pathway. It was also discovered that miR-760 directly targets HM13 and has tumor-suppressive effects in breast cancer.
CELL DEATH & DISEASE
(2022)
Article
Chemistry, Multidisciplinary
Hae Min Lee, Dong Wook Choi, Seahyun Kim, Aro Lee, Minseo Kim, Yeon Jin Roh, Young Ho Jo, Hwa Yeon Cho, Ho-Jae Lee, Seung-Rock Lee, Lionel Tarrago, Vadim N. Gladyshev, Ji Hyung Kim, Byung Cheon Lee
Summary: This study developed a biological sensor, tpMetROG, for quantifying oxidized methionine in target proteins, demonstrating its versatile application in various proteins.
Article
Biochemistry & Molecular Biology
Andrea Madabeni, Laura Orian
Summary: This study investigates the reductive mechanisms of dimethyl selenoxide and dimethyl sulfoxide to gain insights into the role of the chalcogen on the reaction substrate. The key role of sulfurane and selenurane intermediates is emphasized. Additionally, the ability of these systems to oxidize selenols rather than thiols and form selenyl sulfide bridges is explored computationally.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Long Cui, Fangyan Zheng, Dedi Zhang, Changxing Li, Miao Li, Jie Ye, Yuyang Zhang, Taotao Wang, Bo Ouyang, Zonglie Hong, Zhibiao Ye, Junhong Zhang
Summary: In this study, it was found that SlMsrB2 interacts with CAT2 and RBCS3B to regulate ROS accumulation, enhance drought tolerance, and promote chlorophyll accumulation in tomato plants.