Article
Chemistry, Multidisciplinary
Jingwei Meng, Teng Fei, Jinxiong Cai, Qi Lai, Jinya Zhang, Siping Pang, Chunlin He
Summary: In this work, a novel energetic molecule DATNBP-2 was designed and synthesized, which exhibited high density, high thermal decomposition temperature, and excellent detonation performance. It outperformed traditional explosives in terms of stability.
MATERIALS CHEMISTRY FRONTIERS
(2023)
Article
Biochemistry & Molecular Biology
Yixue Wei, Chao Ren, Wenqiang Cai, Xianbing Xu, Zhenyu Wang, Ming Du, Chao Wu
Summary: This study presents a facile approach to enhance the heat stability of soy proteins by preheating at alkaline pH values. The modified soy proteins showed improved anti-aggregation properties, even at high concentrations. The unfolding of the protein structures and reduced exposure of active sites contributed to the enhanced thermal stability.
Article
Chemistry, Multidisciplinary
Nur Atiqah Lyana Nor Ashikin, Siti Fatimah Zaharah Mohd Fuzi, Shoriya Aruni Abdul Manaf, Nor Hasmaliana Abdul Manas, Shalyda Md Shaarani Md Nawi, Rosli Md Illias
Summary: There are various parameters that can influence the properties of immobilized cells. In this study, graphene oxide was used as a carrier and adsorption was employed to enhance the production of thermostable xylanase. Immobilization of cells improved protein expression, reduced cell lysis, and enhanced plasmid stability.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Bo Zhu, Cheng Qian, Haoxuan Tang, Tetsuya Kitaguchi, Hiroshi Ueda
Summary: In this study, a thermostable GUS enzyme switch based on the mutant DLW was developed by disrupting a conserved salt bridge, which showed up to 1.8-fold signal-to-background ratio at high temperatures. Molecular dynamics simulations were used to predict the structural changes, highlighting DLW as a versatile tool for developing thermostable immunosensors.
Article
Biochemical Research Methods
Roxana Disela, Olivier Le Bussy, Geoffroy Geldhof, Martin Pabst, Marcel Ottens
Summary: Mass-spectrometry-based proteomics is used to monitor purification processes and detect host cell proteins in the final drug substance. It provides unbiased identification of individual host cell proteins. Proteomics can provide qualitative and quantitative information on the host cell proteome before purification, enabling a more rational design of purification strategies. This study presents an extensive proteomic characterization of two commonly used E. coli host cell strains and integrates subunit information and post-translational modifications.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Multidisciplinary Sciences
Ahmed R. Elbestawy, Hany F. Ellakany, Hatem S. Abd El-Hamid, Madiha S. Ibrahim, Ahmed R. Gado, Nagwa S. Mustafa, Ihab M. Moussa, Khalid S. Al-Maary, Dalia S. Al-Sarar, Hanan O. Alshammari, Turki M. Dawoud, Hassan A. Hemeg, Hussein M. Galal
Summary: This study evaluated the cross-protection ability of live attenuated E. coli vaccine against heterologous APEC serotypes, showing significant protection but subtle differences in clinical signs, post-mortem lesions, mortality rates, E. coli re-isolation, histopathological examination, final body weight, and feed conversion ratio.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Nabeel Ali, Ayesha Aiman, Anas Shamsi, Imtaiyaz Hassan, Mohammad Shahid, Naseem A. Gaur, Asimul Islam
Summary: The study identified a xylose reductase obtained from the genome of a thermophilic fungus and successfully purified and characterized its structure and function. The enzyme showed efficient conversion of xylose to xylitol at higher temperatures, indicating its potential application in fermentation industries. This research highlights the importance of using lignocellulosic feedstock for the production of value-added products.
Article
Virology
Maik Wolfram-Schauerte, Nadiia Pozhydaieva, Madita Viering, Timo Glatter, Katharina Hoefer
Summary: This study used transcriptomics and proteomics to investigate the temporal patterns of transcription and protein synthesis during T4 phage infection of E. coli. The research revealed the degradation of E. coli transcripts and the stability of the host proteome during infection. Additionally, the correlation between phage transcriptome and proteome provided insights into post-transcriptional and translational regulation mechanisms.
Article
Microbiology
Jimena Barrero-Canosa, Luyao Wang, Angelah Oyugi, Simon Klaes, Pascal Fischer, Lorenz Adrian, Ulrich Szewzyk, Myriel Cooper
Summary: In this study, a phage isolated from urban wastewater treatment plant effluent was characterized. The phage infects multiple strains of E. coli and has specific protein composition, affecting bacterial metabolism and stress response.
Article
Microbiology
Karen Hon, Sha Liu, Sophie Camens, George Spyro Bouras, Alkis James Psaltis, Peter-John Wormald, Sarah Vreugde
Summary: This study aimed to isolate and characterize phage as an alternative treatment option for multi-drug resistant E. coli infections. The newly isolated APTC-EC-2A phage showed promising properties, including stability at a broad pH range, ability to kill E. coli at different temperatures, and effectiveness against both planktonic and biofilm forms of the bacteria. Electron microscopy and genomic sequencing revealed that the phage belongs to the Myoviridae family and has lytic nature.
Article
Chemistry, Applied
Rongwei Han, Runjia Shi, Zhongna Yu, Harvey Ho, Qijing Du, Xueheng Sun, Jun Wang, Hongning Jiang, Rongbo Fan, Yongxin Yang
Summary: The proteome of casein micelles (CMs) in different types of milk changes following heat treatment, with some proteins showing similar changes across species while others differ. Understanding the distribution and variations of the proteomes of CMs following heat treatment is important in identifying proteins that are dissociated and attached to CMs from different dairy species.
Article
Biotechnology & Applied Microbiology
Thomas Bessonnet, Aline Mariage, Jean-Louis Petit, Virginie Pellouin, Adrien Debard, Anne Zaparucha, Carine Vergne-Vaxelaire, Veronique de Berardinis
Summary: The study identified a thermostable nitrilase Nit(phym) from Paraburkholderia phymatum, which exhibited high operational stability, thermostability, and a broad pH range. This nitrilase also displayed high resistance and tolerance, enabling efficient conversion of various nitriles.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Yasmine Gomaa, Chandana Kolluru, Mikolaj Milewski, Dinah Lee, Jingtao Zhang, Robert Saklatvala, Mark R. Prausnitz
Summary: The study proposes a thermostable, easy-to-administer microneedle patch for rapid release of oxytocin, suitable for healthcare workers with limited training. This new method extends the stability time of oxytocin and improves access to this life-saving medicine.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Biotechnology & Applied Microbiology
Xinglong Wang, Beichen Zhao, Jianhui Du, Yameng Xu, Xuewen Zhu, Jingwen Zhou, Shengqi Rao, Guocheng Du, Jian Chen, Song Liu
Summary: This study aimed to improve the thermostability and secretory expression of Streptomyces mobaraenesis transglutaminase (smTG) in E. coli. A combined strategy was employed, including N-terminal modification, co-expression with chaperones, mRNA folding energy optimization of signal peptide, and lipoprotein deletion. The results showed significant improvements in the activity and secretion of smTG, which are of great importance for enzyme application and evolution.
MICROBIAL CELL FACTORIES
(2022)
Article
Chemistry, Organic
Jatinder Singh, Richard J. J. Staples, Jeanne M. Shreeve
Summary: A strategy for synthesizing nitrogen-rich energetic materials with good properties is reported, taking advantage of the synergetic effects of inter- or intramolecular hydrogen bonds. A new thermostable and insensitive material, 1,2-dihydrazono-1,2-di(1H-tetrazol-5-5-yl)ethane, was obtained from the reaction of dioxime with hydrazinehydrate. The material has reduced acidity, low solubility in water, high nitrogen content (76%), high thermal stability (275°C), and insensitivity to external stimuli.
