Review
Chemistry, Analytical
Tsukuru Minamiki, Yuki Ichikawa, Ryoji Kurita
Review
Chemistry, Analytical
Hiroka Sugai, Shunsuke Tomita, Ryoji Kurita
ANALYTICAL SCIENCES
(2020)
Article
Chemistry, Analytical
Sima Chaudhari, Tomoyuki Kamata, Naoshi Kojima, Michinori Sumimoto, Kapaettu Satyamoorthy, Ryoji Kurita, Dai Kato
SENSORS AND ACTUATORS B-CHEMICAL
(2020)
Article
Chemistry, Multidisciplinary
Shunsuke Tomita, Hiroka Sugai, Sayaka Ishihara, Takuya Hosokai, Ryoji Kurita
Article
Chemistry, Analytical
Mari Okada, Hiroka Sugai, Shunsuke Tomita, Ryoji Kurita
Article
Instruments & Instrumentation
Shunsuke Tomita, Ryoji Kurita
Summary: The study introduces a pattern-recognition-based protein sensing approach using a simple one-component array composed of charged poly-L-lysine modified with environment-responsive dansyl fluorophores for the identification of proteases. By dissolving the array of PLL-Dnc in buffer solutions with different pH values, fluorescent patterns unique to each protease are produced, enabling the identification of eight proteases through multivariate analysis. The method allows discrimination of mixtures of trypsin and alpha(1)-antitrypsin (ATT) with different compositions, and is expected to have broad applications in screening protein-protein complexes formation and disease-associated protease detection.
SENSORS AND MATERIALS
(2021)
Article
Chemistry, Analytical
Hiroki Okada, Masahiro Mimura, Shunsuke Tomita, Ryoji Kurita
Summary: The study introduces a novel strategy of using chemical additives to modulate probe/analyte interactions for constructing pattern-recognition-based sensing systems. The enhanced fluorescence of dansyl-modified cationic poly-L-lysine upon binding to proteins generates response patterns that accurately identify structurally similar albumin homologues and mammalian cells through multivariate analysis. This inexpensive and accessible strategy offers new opportunities for bioanalytes detection.
ANALYTICAL SCIENCES
(2021)
Article
Chemistry, Analytical
Shota Takahashi, Hiroyuki Yanagisawa, Tomoyuki Kamata, Dai Kato, Ryoji Kurita, Shunsuke Shiba, Osamu Niwa
Summary: A research team developed carbon film electrodes using UBM and ECR sputtering equipment for electroanalysis of heavy metal ions. Different methods were used for detection of different heavy metal ions, achieving good detection limits suitable for environmental analysis.
Article
Plant Sciences
Tatsunosuke Tomita, Renu Wadhwa, Sunil C. Kaul, Ryoji Kurita, Naoshi Kojima, Yoshiaki Onishi
Summary: Withanolide derivatives, particularly 3 beta mWi-A, have been shown to lengthen the circadian clock, upregulate the expression of key genes involved in circadian rhythms, and act as an inverse agonist for RORa. These findings suggest that 3 beta mWi-A may serve as a novel modulator of circadian rhythms.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Chemistry, Multidisciplinary
Masahiro Mimura, Shunsuke Tomita, Yoichi Shinkai, Takuya Hosokai, Hiroyuki Kumeta, Tomohide Saio, Kentaro Shiraki, Ryoji Kurita
Summary: Recent studies have shown that DNA quadruplex folding plays a key role in the formation of liquid droplets through interactions with linker histone H1, inducing liquid-liquid phase separation. Quadruplex DNA structures promote droplet formation and decrease molecular motility within the droplets.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Cell Biology
Masahiro Mimura, Shunsuke Tomita, Hiroka Sugai, Yoichi Shinkai, Sayaka Ishihara, Ryoji Kurita
Summary: This study demonstrates the roles of DNA components in liquid-liquid phase separation of proteins and DNAs, including electrostatic interactions between phosphate groups and deoxyribose, as well as interactions involving nucleobases and deoxyriboses. These interactions promote the generation of spherical liquid droplets and modulate the density of DNA within the droplets under low-salt conditions. Additionally, non-spherical assemblies with both mobile and immobile fractions were observed at higher concentrations of a polycationic peptide, linker histone H1, for all DNA oligomers.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Analytical
Hiroyuki Yanagisawa, Kenji Sasaki, Yoshimi Sasaki, Asumi Omata, Rina Ichino, Shigehiko Fujimaki
Summary: This study introduces a simple method to screen for the presence of TBBPA in polymers using a colorimetric measurement, avoiding the need for complex processes. The method showed a detection limit of approximately 2 mg/kg and a reproducibility of around 3%, making it a quick and efficient way to detect TBBPA in various polymers.
ANALYTICAL SCIENCES
(2021)
Article
Multidisciplinary Sciences
Katsuyuki Takeuchi, Hiroyuki Yanagisawa, Yukiko Kurosawa, Yoritsugu Iida, Kosuke Kawai, Shigehiko Fujimaki
Summary: This study investigated the degradation of SARS-CoV-2 specific RNA using numerical modeling based on nucleic acid amplification test (NAAT) results. The precision of the measurement was verified using relative standard deviation (RSD) of repeated measurements. The results showed that RNA degradation curves followed a first-order reaction model and the Arrhenius model could be used to predict RNA degradation behavior. These findings provide practical guidance for RNA sample management in NAAT of non-human samples.
Article
Chemistry, Multidisciplinary
Shunsuke Tomita, Hiroyuki Kusada, Naoshi Kojima, Sayaka Ishihara, Koyomi Miyazaki, Hideyuki Tamaki, Ryoji Kurita
Summary: This study developed a sensing system based on the chemical-nose strategy to transduce the characteristics of microbiota into fluorescence patterns. By using pattern-recognition algorithms to analyze the fluorescence patterns, different intestinal bacterial strains can be identified, and obesity-associated and sleep disorder-associated gut microbiota can be detected.
Article
Chemistry, Analytical
Ryo Nishihara, Ryoji Kurita
Summary: The study developed a luciferase-independent copper detection platform using a novel luminogenic caged coelenterazine analogue (TPA-H1) to detect free copper ions in serum with high specificity and sensitivity. This new method can be a simple mix-and-read approach for quantitatively analyzing copper ions.