Article
Biology
Almut S. Eisele, Jason Cosgrove, Aurelie Magniez, Emilie Tubeuf, Sabrina Tenreira Bento, Cecile Conrad, Fanny Cayrac, Tamar Tak, Anne-Marie Lyne, Jos Urbanus, Leila Perie
Summary: This study used cellular barcoding to trace the differentiation of mouse hematopoietic stem and progenitor cells (HSPCs) after exposure to EPO. The researchers found that EPO can directly act on multipotent progenitor cells (MPP) and modulate fate by remodeling the clonal composition of the MPP pool.
Article
Engineering, Biomedical
Aidan E. Gilchrist, Julio F. Serrano, Mai T. Ngo, Zona Hrnjak, Sanha Kim, Brendan A. C. Harley
Summary: Biomaterial platforms are crucial in stem cell biomanufacturing, with GelMAL hydrogel providing a versatile route for encapsulating stem cell populations and mimicking the bone marrow niche. The maleimide-functionalized gelatin allows for stable crosslinking without reactive oxide species generation, demonstrating potential for artificial stem cell niche construction.
ACTA BIOMATERIALIA
(2021)
Article
Cell & Tissue Engineering
Karthikeyan Kandasamy, Nuryanti Binti Johana, Lay Geok Tan, Yvonne Tan, Julie Su Li Yeo, Nur Nazneen Binte Yusof, Zhihui Li, Jiayu Koh, Florent Ginhoux, Jerry K. Y. Chan, Mahesh Choolani, Citra N. Z. Mattar
Summary: Maternal immune cells trafficked into the recipient's body through the placenta can directly influence immune responses to donor cells, limiting donor cell chimerism. Depleting maternal dendritic cells reduces recipient alloresponsiveness and enhances donor cell chimerism, suggesting a novel approach to improving donor cell tolerance.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Oncology
Shaima Jabbar, Parker Mathews, Xiaobei Wang, Pasupathi Sundaramoorthy, Emily Chu, Sadhna O. Piryani, Shengli Ding, Xiling Shen, Phuong L. Doan, Yubin Kang
Summary: This study reveals that the thioredoxin-1 (TXN1)-TP53 axis plays a regulatory role in the biological functions of hematopoietic stem/progenitor cells (HSPCs). Deletion of TXN1 activates the TP53 signaling pathway and impairs the capacity of HSPCs to reconstitute hematopoiesis. Moreover, knockout of TXN1 renders HSPCs more sensitive to radiation, while treatment with recombinant TXN1 promotes the proliferation and expansion of HSPCs.
EXPERIMENTAL HEMATOLOGY & ONCOLOGY
(2022)
Article
Immunology
Shufeng Luo, Huiling Lin, Lan Zhu, Hai-Tian Chen, Siqian Yang, Jinheng Li, Mingyu Liu, Limin Zheng, Chong Wu
Summary: Under stress conditions, HSPCs can interpret danger signals into cytokine signals to modify hematopoietic output. A flow cytometry-based method was developed to assess intracellular cytokines in HSPCs, revealing distinct regulatory modes of cytokine production depending on subset, cytokine, disease, organ, and species. This technical advance helps to better understand HSPC heterogeneity based on differential cytokine production.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Hematology
Alice Tang, Ana Nicolle Strat, Mahmudur Rahman, Helen Zhang, Weili Bao, Yunfeng Liu, David Shi, Xiuli An, Deepa Manwani, Patricia Shi, Karina Yazdanbakhsh, Avital Mendelson
Summary: Studies have found that murine SCD MSCs exhibit altered gene signatures, reduced stem cell properties, and increased oxidative stress, leading to decreased HSC maintenance ability. The activation of Toll-like receptor-4 through p65 in MSCs further exacerbates MSC dysfunction in SCD.
Article
Multidisciplinary Sciences
Lijian Shao, Na Yoon Paik, Mark A. Sanborn, Thilinie Bandara, Anjali Vijaykumar, Kilian Sottoriva, Jalees Rehman, Cesar Nombela-Arrieta, Kostandin V. Pajcini
Summary: Notch signaling is crucial for the emergence and development of definitive hematopoietic stem cells (HSCs) in the embryo and fetal liver. This study reveals endothelial Jagged1 (Jag1) as a critical factor in early fetal liver vascular development but not essential for fetal HSC expansion. Jag1 is expressed in various hematopoietic cells in the fetal liver, including HSCs, but lost in adult bone marrow HSCs. Deletion of hematopoietic Jag1 does not impact fetal liver development, but results in a significant transplantation defect in fetal liver HSCs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Murat Tekguc, James Badger Wing, Motonao Osaki, Jia Long, Shimon Sakaguchi
Summary: Regulatory T cells reduce the expression of CD80/CD86 on antigen-presenting cells through CTLA-4-dependent trogocytosis, inhibiting T cell immune responses. This mechanism can have dual suppressive effects, limiting CD80/CD86 costimulation to naive T cells and increasing free PD-L1 available for the inhibition of PD-1-expressing effector T cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Frauke Gotzhein, Tim Aranyossy, Lars Thielecke, Tanja Sonntag, Vanessa Thaden, Boris Fehse, Ingo Mueller, Ingmar Glauche, Kerstin Cornils
Summary: In this study, a murine model of HSCT was used to investigate the contribution of different hematological subpopulations to the reconstitution process and the impact of age on this process. The results showed that hematopoietic stem cells and multipotent progenitors played a major role in reconstitution, independent of age. The reconstitution patterns were stable in all age groups. These findings support the expansion of HSCT in older individuals as a promising strategy for treating hematological diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Hematology
Michihiro Hashimoto, Terumasa Umemoto, Ayako Nakamura-Ishizu, Takayoshi Matsumura, Tomomasa Yokomizo, Maiko Sezaki, Hitoshi Takizawa, Toshio Suda
Summary: The role of autophagy in neonatal HSCs is unclear, but it has an impact on the regulation of adult HSCs.
Article
Anatomy & Morphology
Sheida Farrokhi, Fattah Sotoodehnejadnematalahi, Anwar Fathollahi, Mostafa Haji Molla Hoseini, Seyed Mahmoud Hashemi, Farshid Yeganeh
Summary: The use of chitosan film as a three-dimensional culture strategy significantly impacts the production of immunosuppressive factors by MSCs in vitro through increased secretion of TGF-β and IL-10, as well as arginase activity.
Review
Immunology
Carsten Riether
Summary: Adult bone marrow hematopoietic stem cells (HSCs) maintain their function through interactions with regulatory T cells (Tregs). In leukemia, these regulatory mechanisms are disrupted, leading to compromised treatment outcomes.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Wendi Guo, Colleen Wu
Summary: Currently, there is a lack of universally accepted markers for isolating skeletal stem cells (SSCs). Therefore, researchers continue to use bone marrow-derived mesenchymal stem cells (BMSCs) as a model to study SSCs and multipotent mesenchymal progenitors (MMPs). However, the common isolation procedures for BMSCs often result in a high percentage of hematopoietic cells, which can complicate data interpretation. In this study, a method using low oxygen tension or hypoxia is described to selectively eliminate CD45+ cells in BMSC cultures, reducing hematopoietic contaminants and enhancing MMPs and putative SSCs.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Microbiology
Shunchuan Zhang, Lauren E. Springer, Han-Zhi Rao, Renee G. Espinosa Trethewy, Lindsey M. Bishop, Meaghan H. Hancock, Finn Grey, Christopher M. Snyder
Summary: This study reveals that cytomegalovirus relies on hematopoietic cells for dissemination after infection, while cell-mediated immune responses restrict the virus to infected hematopoietic cells during dissemination.
Article
Immunology
Haoyue Liang, Weichao Fu, Wenying Yu, Zhijie Cao, Ertao Liu, Fanfan Sun, Xiaodong Kong, Yingdai Gao, Yuan Zhou
Summary: This study compares the mitochondrial functions of different subsets of lymphocytes at different stages of differentiation and activation. B cells were found to have relatively high mitochondrial metabolic activity, while mature B cells showed lower levels. Naive CD4(+) T cells also exhibited relatively high mitochondrial metabolism. Memory CD8(+) T cells had high mitochondrial mass and membrane potential but low mitophagy levels. The study also analyzed the involvement of autophagy-related genes in different lymphocyte subsets, highlighting the heterogeneity of mitophagy pathways.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
S. Kovats, S. Turner, A. Simmons, T. Powe, E. Chakravarty, J. Alberola-Ila
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2016)
Article
Hematology
Esther Carreras, Sean Turner, Mark Barton Frank, Nicholas Knowlton, Jeanette Osban, Michael Centola, Chae Gyu Park, Amie Simmons, Jose Alberola-Ila, Susan Kovats
Review
Immunology
Taishan Hu, Idoia Gimferrer, Jose Alberola-Ila
Article
Immunology
Jens-Peter Holst Lauritsen, Sridevi Kurella, Sang-Yun Lee, Juliette M. Lefebvre, Michele Rhodes, Jose Alberola-Ila, David L. Wiest
JOURNAL OF IMMUNOLOGY
(2008)
Article
Immunology
Idoia Gimferrer, Taishan Hu, Amie Simmons, Chi Wang, Abdallah Souabni, Meinrad Busslinger, Timothy P. Bender, Gabriela Hernandez-Hoyos, Jose Alberola-Ila
JOURNAL OF IMMUNOLOGY
(2011)
Article
Immunology
Taishan Hu, Hongcheng Wang, Amie Simmons, Sandra Bajana, Ying Zhao, Susan Kovats, Xiao-Hong Sun, Jose Alberola-Ila
JOURNAL OF IMMUNOLOGY
(2013)
Article
Immunology
Taishan Hu, Amie Simmons, Joan Yuan, Timothy P. Bender, Jose Alberola-Ila
Article
Multidisciplinary Sciences
Taishan Hu, Idoia Gimferrer, Amie Simmons, David Wiest, Jose Alberola-Ila
Article
Multidisciplinary Sciences
Rafael Fenutria, Vanesa G. Martinez, Ines Simoes, Jorge Postigo, Victor Gil, Mario Martinez-Florensa, Jordi Sintes, Rodrigo Naves, Kevin S. Cashman, Jose Alberola-Ila, Manel Ramos-Casals, Gloria Soldevila, Chander Raman, Jesus Merino, Ramon Merino, Pablo Engel, Francisco Lozano
Article
Multidisciplinary Sciences
Ryuji Iida, Robert S. Welner, Wanke Zhao, Josee Alberola-lla, Kay L. Medina, Zhizhuang Joe Zhao, Paul W. Kincade
Article
Oncology
Ines T. Simoes, Fernando Aranda, Sergi Casado-Llombart, Maria Velasco-de Andres, Cristina Catala, Pilar Alvarez, Marta Consuegra-Fernandez, Marc Orta-Mascaro, Ramon Merino, Jesus Merino, Jose Alberola-Ila, Gloria Gonzalez-Aseguinolaza, Esther Carreras, Vanesa Martinez, Francisco Lozano
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2020)
Article
Immunology
Liangyue Qian, Sandra Bajana, Constantin Georgescu, Vincent Peng, Hong-Cheng Wang, Indra Adrianto, Marco Colonna, Jose Alberola-Ila, Jonathan D. Wren, Xiao-Hong Sun
JOURNAL OF EXPERIMENTAL MEDICINE
(2019)
Article
Immunology
Susannah D. Barbee, Jose Alberola-Ila
INTERNATIONAL IMMUNOLOGY
(2006)
Article
Immunology
SD Barbee, J Alberola-Ila
JOURNAL OF IMMUNOLOGY
(2005)