期刊
BLOOD
卷 118, 期 19, 页码 5130-5140出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2010-07-295626
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资金
- Association pour la Recherche contre le Cancer
- Comites departementaux de Saone-et-Loire et du Rhone de la Ligue nationale contre le cancer
- Institut National du Cancer [INCA ACI-63-04, ACI 2007-2009, PL116, PL-969-017]
- Breast Cancer Research Foundation
- BioPole program DEMINAP
- Region Rhone-Alpes and Association pour la Recherche sur le Cancer
Absent in peripheral tissues during homeostasis, human plasmacytoid dendritic cells (pDCs) are described in inflamed skin or mucosa. Here, we report that, unlike blood pDCs, a subset of tonsil pDCs express functional CCR6 and CCR10, and their respective ligands CCL20 and CCL27 are detected in inflamed epithelia contacting blood dendritic cell antigen 2(+) pDCs. Moreover, pDCs are recruited to imiquimod-treated skin tumors in WT but not CCR6-deficient mice, and competitive adoptive transfers reveal that CCR6-deficient pDCs are impaired in homing to inflamed skin tumors after intravenous transfer. On IL-3 culture, CCR6 and CCR10 expression is induced on human blood pDCs that become responsive to CCL20 and CCL27/CCL28, respectively. Interestingly, unlike myeloid DC, blood pDCs initially upregulate CCR7 expression and CCL19 responsiveness on IL-3 +/- CpG-B and then acquire functional CCR6 and CCR10. Finally, IL-3-differentiated CCR6(+) CCR10(+) pDCs secrete high levels of IFN-alpha in response to virus. Overall, we propose an unexpected pDCs migratory model that may best apply for mucosal-associated lymphoid tissues. After CCR7-mediated extravasation into lymphoid tissues draining inflamed epithelia, blood pDCs may be instructed to up-regulate CCR6 and/or CCR10 allowing their homing into inflamed epithelia (in mucosae or skin). At this site, pDCs can then produce IFN-alpha contributing to pathogen clearance and/ or local inflammation. (Blood. 2011;118(19):5130-5140)
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