Article
Multidisciplinary Sciences
Zihan Tang, Yanyan Xu, Yun Tan, Hui Shi, Peipei Jin, Yunqi Li, Jialin Teng, Honglei Liu, Haoyu Pan, Qiongyi Hu, Xiaobing Cheng, Junna Ye, Yutong Su, Yue Sun, Jianfen Meng, Zhuochao Zhou, Huihui Chi, Xuefeng Wang, Junling Liu, Yong Lu, Feng Liu, Jing Dai, Chengde Yang, Saijuan Chen, Tingting Liu
Summary: Aberrant coagulation and thrombosis are associated with severe COVID-19 post-SARS-CoV-2 infection. The E protein is found to be associated with coagulation disorders in COVID-19 patients and directly enhances platelet activation and thrombosis through a CD36/p38 MAPK/NF-kB signaling axis.
NATURE COMMUNICATIONS
(2023)
Article
Hematology
Xiamin Wang, Guangyu Wei, Yangyang Ding, Xiang Gui, Huan Tong, Xiaoqi Xu, Sixuan Zhang, Zengtian Sun, Wen Ju, Yue Li, Ruosi Yao, Qingyu Wu, Zhihao Lu, Chunling Fu, Zhenyu Li, Si Zhang, Elizabeth E. Gardiner, Robert K. Andrews, Hu Hu, Lingyu Zeng, Jianlin Qiao, Kailin Xu
Summary: This study reveals a novel role of protein tyrosine phosphatase nonreceptor type 22 (PTPN22) in platelet function and arterial thrombosis, identifying new potential targets for future prevention of thrombotic or cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Lisa Maria Metz, Margitta Elvers
Summary: PANX1 channels play a crucial role in platelet activation and thrombus formation, and their activation is regulated by phosphorylation. The Src-GPVI signaling pathway is identified as the main pathway for PANX1 activation, with PKC and Akt playing a minor role.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Paresh P. Kulkarni, Mohammad Ekhlak, Vipin Singh, Vikas Kailashiya, Nitesh Singh, Debabrata Dash
Summary: A comprehensive analysis showed that beta-oxidation of fatty acids is crucial for maintaining ATP levels and plays a critical role in activated platelet responses. Therefore, targeting fatty acid oxidation may be a promising strategy for developing novel antiplatelet agents.
Article
Biology
Richard Sando, Thomas C. Sudhof
Summary: Latrophilin-2 and Latrophilin-3 play essential roles in synapse formation in the hippocampus by requiring GPCR signaling to activate the classical GPCR-signaling pathway.
Article
Chemistry, Multidisciplinary
Yonghao Mi, Enliang Wang, Zack Dube, Tian Wang, A. Y. Naumov, D. M. Villeneuve, P. B. Corkum, Andre Staudte
Summary: Using near-infrared, femtosecond laser pulses and coincidence momentum imaging, it is found that the dominant channel after photoionization of a deuterium molecular dimer (D-2-D-2) is the ejection of a deuterium atom within a few hundred femtoseconds, leading to the formation of D-3(+). This pathway of D-3(+) formation from ultracold D-2-D-2 gas may provide insights into the high abundance of H-3(+) in the interstellar medium.
Article
Biochemistry & Molecular Biology
Dan Shu, Ying Zhu, Meng Lu, Ao-Di He, Jiang-Bin Chen, Ding-Song Ye, Yue Liu, Xiang-Bin Zeng, Rong Ma, Zhang-Yin Ming
Summary: Sanguinarine demonstrates antiplatelet activity by inhibiting thrombus formation and platelet activation through multiple molecular mechanisms. This indicates its potential clinical value in treating cardiovascular diseases involving excessive platelet activation.
Article
Food Science & Technology
Barbara G. de Freitas, Natalia G. Hoesch, Leandro M. Pereira, Tereza C. Barbosa, Gisele Picolo, Yara Cury, Vanessa O. Zambelli
Summary: Crotalphine, a peptide from the venom of South American rattlesnake, induces potent and long-lasting antinociceptive effect through the activation of peripheral opioid receptors. This effect is dependent on the PKC zeta-ERK signaling pathway, inhibition of which blocks the analgesic effect of Crotalphine. These findings suggest the PKC zeta-MAPK axis as a potential target for pain control.
Article
Hematology
Ilaria De Simone, Constance C. F. M. J. Baaten, Jonathan M. Gibbins, Hugo Ten Cate, Johan W. M. Heemskerk, Chris I. Jones, Paola E. J. van der Meijden
Summary: This study found that previously activated platelets can be reactivated through different receptors and contribute to thrombus formation. Reactivated platelets can regain their ability to participate in thrombus formation under flow conditions.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Biochemistry & Molecular Biology
Hyuk-Woo Kwon, Sung Dae Kim, Man Hee Rhee, Jung-Hae Shin
Summary: Platelets play a significant role in hemostasis but can also contribute to the development of cardiovascular diseases. Controlling platelet function is important in preventing thrombosis. A compound called 5-hydroxyindolin-2-one (5-HI) has been found to inhibit human platelet aggregation without cytotoxicity and suppress intracellular Ca2+ concentration. It also regulates various pathways to inhibit thromboxane A(2) production and alpha IIb beta 3 action, suggesting its potential as a substance for preventing thrombosis-mediated thrombosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Hematology
Charlie A. Coupland, Leigh Naylor-Adamson, Zoe Booth, Thomas W. Price, Helio M. Gil, George Firth, Michelle Avery, Yusra Ahmed, Graeme J. Stasiuk, Simon D. J. Calaminus
Summary: Approximately 17.3% of the global population suffers from zinc (Zn2+) deficiency, which leads to increased bleeding due to impaired hemostasis. Zinc ion (Zn2+) has the ability to modulate platelet signaling through the endothelial-derived prostacyclin I2 (PGI2) pathway, affecting thrombus formation and hemostasis.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Multidisciplinary Sciences
Yagmur Azbazdar, Nydia Tejeda-Munoz, Julia C. Monka, Alex Dayrit, Grace Binder, Gunes Ozhan, Edward M. De Robertis
Summary: Activation of Wnt signaling leads to macropinocytosis, which promotes tumor progression. Addition of the second messenger lipid sn-1,2 DAG induces macropinocytosis rapidly. Moreover, DAG addition enhances the effects of Wnt3a recombinant protein and GSK3 inhibitor LiCl in luciferase transcriptional reporter assays. In colorectal carcinoma cells with constitutive Wnt signaling, DAG addition increases nuclear beta-catenin levels, which can be partially inhibited by a macropinocytosis inhibitor. DAG also expands multivesicular bodies marked by CD63 protein. In vivo, microinjection of DAG induces Wnt-like twinned body axes in Xenopus embryos when co-injected with small amounts of LiCl.
Article
Hematology
Xiang Gui, Xiang Chu, Yuwei Du, Yuhan Wang, Sixuan Zhang, Yangyang Ding, Huan Tong, Mengdi Xu, Yue Li, Wen Ju, Zengtian Sun, Zhenyu Li, Lingyu Zeng, Kailin Xu, Jianlin Qiao
Summary: PDE5A deficiency prolongs bleeding time and delays arterial and venous thrombus formation. It inhibits platelet aggregation and activation, reduces phosphatidylserine exposure, calcium mobilization, and reactive oxygen species production, increases intracellular cGMP level, and modulates platelet function and thrombus formation.
THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Biochemistry & Molecular Biology
Norikiyo Honzawa, Kei Fujimoto, Masaki Kobayashi, Daisuke Kohno, Osamu Kikuchi, Hiromi Yokota-Hashimoto, Eri Wada, Yuichi Ikeuchi, Yoko Tabei, Gerald W. Dorn Ii, Kazunori Utsunomiya, Rimei Nishimura, Tadahiro Kitamura
Summary: Pkc delta plays a crucial role in glucagon secretion from pancreatic alpha-cells, and its depletion or inhibition leads to a decrease in glucagon secretion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Plant Sciences
Yuan Yee Lee, Sung Dae Kim, Seung-Chun Park, Man Hee Rhee
Summary: This article summarizes the therapeutic effects of ginseng on cardiovascular diseases, focusing on its influence on platelet aggregation, plaque formation, and thrombus formation.
JOURNAL OF GINSENG RESEARCH
(2022)
Editorial Material
Cardiac & Cardiovascular Systems
Judith M. E. M. Cosemans
EUROPEAN HEART JOURNAL
(2022)
Article
Immunology
M. F. A. Karel, M. G. C. H. Roosen, B. M. E. Tullemans, C. Eleana Zhang, J. Staals, J. M. E. M. Cosemans, R. R. Koenen
Summary: Cerebral small vessel disease (cSVD) is a major cause of cognitive decline and stroke. This study found that the neutrophil activation marker MPO is elevated in cSVD patients and highlighted platelet and neutrophil markers as associated with cSVD.
JOURNAL OF NEUROIMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Linsey J. F. Peters, Constance C. F. M. J. Baaten, Sanne L. Maas, Chang Lu, Magdolna Nagy, Natalie J. Jooss, Kiril Bidzhekov, Donato Santovito, Daniel Moreno-Andres, Joachim Jankowski, Erik A. L. Biessen, Yvonne Doering, Johan W. M. Heemskerk, Christian Weber, Marijke J. E. Kuijpers, Emiel P. C. van der Vorst
Summary: This study demonstrates for the first time that miR-26b attenuates platelet adhesion and aggregation, possibly through Src and EGFR signaling.
Letter
Hematology
Ejaife O. Agbani, Ingeborg Hers, Alastair W. Poole
Letter
Hematology
Justin S. Trory, Attila Munkacsi, Kamila M. Sledz, Jordan Vautrinot, Lucy J. Goudswaard, Molly L. Jackson, Kate J. Heesom, Samantha F. Moore, Alastair W. Poole, Behnam Nabet, Varinder K. Aggarwal, Ingeborg Hers
Letter
Immunology
Alejandro J. J. Brenes, Angus I. I. Lamond, Doreen A. A. Cantrell
Article
Multidisciplinary Sciences
Venkat M. K. Vadlamani, Kavinda K. J. Gunasinghe, Xavier W. Chee, Taufiq Rahman, Matthew T. Harper
Summary: CD39 is a key regulator that metabolizes extracellular ATP and ADP to AMP. This study demonstrates that soluble, recombinant CD39 exhibits substrate inhibition with ADP or ATP as the substrate. High concentrations of ATP or ADP significantly reduce CD39 activity. In contrast, inhibition is not observed with UDP or UTP as substrates. Understanding the substrate inhibition of CD39 is important for interpreting CD39 activity studies and investigations into drugs modulating CD39 activity.
SCIENTIFIC REPORTS
(2023)
Article
Hematology
Claudia Schoenichen, Samantha J. Montague, Sanne L. N. Brouns, James J. Burston, Judith M. E. M. Cosemans, Kerstin Jurk, Beate E. Kehrel, Rory R. Koenen, Fionnuala Ni Ainle, Valerie B. O'Donnell, Oliver Soehnlein, Steve P. Watson, Marijke J. E. Kuijpers, Johan W. M. Heemskerk, Magdolna Nagy
Summary: This study reveals the key interaction mechanisms between platelets and neutrophils in arterial thrombus formation. Activated platelet integrin αIIbβ3 plays a role in preventing leukocyte adhesion, while platelet-released substances promote neutrophil activation. These findings provide insights for potential pharmacological intervention.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Isabella Provenzale, Ilaria De Simone, Jonathan M. Gibbins, Johan W. M. Heemskerk, Paola E. J. van der Meijden, Chris I. Jones
Summary: This study investigated the roles of different extracellular matrix proteoglycans in platelet responses and thrombus formation. It was found that one proteoglycan (perlecan) enhanced platelet activation and promoted thrombus formation, but also reduced platelet adhesion under flow. This suggests that proteoglycans play an important role in platelet activation and thrombus formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Xiaojuan Zhao, Dominic Alibhai, Tony G. Walsh, Nathalie Tarassova, Maximilian Englert, Semra Z. Birol, Yong Li, Christopher M. Williams, Chris R. Neal, Philipp Burkard, Stephen J. Cross, Elizabeth W. Aitken, Amie K. Waller, Jose Ballester Beltran, Peter W. Gunning, Edna C. Hardeman, Ejaife O. Agbani, Bernhard Nieswandt, Ingeborg Hers, Cedric Ghevaert, Alastair W. Poole
Summary: Perfusion of megakaryocytes through the mouse lung vasculature generates a large number of functional platelets. This study reveals the mechanisms of thrombopoiesis in lung vasculature and provides insights for large-scale platelet generation.
NATURE COMMUNICATIONS
(2023)
Review
Hematology
Alexandra Yakusheva, Alexandre Slater, Bernard Payrastre, Cedric Garcia, Giorgia D'Italia, Harriet Allan, Judith M. E. M. Cosemans, Matthew Harper, Meinrad Gawaz, Paul Armstrong, Sara Troitino, Silvia Maria Grazia Trivigno, Ulhas P. Naik, Yotis A. Senis
Summary: This article presents the talks and original investigations conducted at the 5th European Platelet Network International Conference, covering recent advances in platelet and megakaryocyte biology.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Hematology
Emma C. Josefsson, Sofia Ramstrom, Johannes Thaler, Marie Lordkipanidze, COAGAPO Study Grp
Summary: The study aimed to identify a set of markers and/or methods to detect and distinguish procoagulant platelets from apoptotic platelets. Using flow cytometry, a combination of three surface markers, including P-selectin (CD62P), phosphatidylserine (recognized by annexin V), and platelet-specific receptor GPIX (CD42a) or αIIb integrin (CD41, GPIIb), was recommended for differentiation. Procoagulant platelets are positive for all three markers, while apoptotic platelets are positive for annexin V and platelet-specific surface receptor(s) but negative for P-selectin.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Hematology
Lucy J. Goudswaard, Christopher M. Williams, Jawad Khalil, Kate L. Burley, Fergus Hamilton, David Arnold, Alice Milne, Phil A. Lewis, Kate J. Heesom, Stuart J. Mundell, Andrew D. Davidson, Alastair W. Poole, Ingeborg Hers
Summary: Patients with COVID-19 have altered platelet function, which is associated with increased risk of thrombosis. Platelet protein expression is different in COVID-19 patients, and platelet functional responses are reduced. Platelets in COVID-19 patients also show increased association with neutrophils. These findings suggest that platelet-driven thromboinflammation may contribute to the increased risk of thrombosis in COVID-19 patients.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Hematology
Sanne L. N. Brouns, Bibian M. E. Tullemans, Cristiana Bulato, Gina Perrella, Elena Campello, Luca Spiezia, Johanna P. van Geffen, Marijke J. E. Kuijpers, Rene van Oerle, Henri M. H. Spronk, Paola E. J. van der Meijden, Paolo Simioni, Johan W. M. Heemskerk
Summary: This study assessed the role of platelets in thrombus and fibrin formation in patients with protein C or protein S deficiency under high-shear flow conditions. The results showed that these patients had impaired platelet activation, thrombus phenotype, and fibrin formation, but unchanged platelet adhesion. The defect may be caused by negative platelet priming.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Hematology
Herm Jan M. Brinkman, Frauke Swieringa, Marleen Zuurveld, Alicia Veninga, Sanne L. N. Brouns, Johan W. M. Heemskerk, Joost C. M. Meijers
Summary: The study demonstrates the beneficial effect of combined use of PCC and FVC in reversing DOAC-induced coagulopathy by increasing thrombin generation, improving clot formation, enhancing clot strength and resistance to lysis.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2022)