Review
Hematology
Serge Mbiandjeu, Alessandra Balduini, Alessandro Malara
Summary: Thrombopoiesis is governed by the response of megakaryocyte cytoskeleton to various stimuli, supporting platelet shedding. New cytoskeleton regulators have emerged as critical components in the dynamic protein network that supports platelet production.
THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Cell Biology
Kirill R. Butov, Elena Y. Osipova, Nikita B. Mikhalkin, Natalia M. Trubina, Mikhail a Panteleev, Kellie R. Machlus
Summary: The study proposes a simple, robust, and applicable method for culturing primary human megakaryocytes from 1 mL of bone marrow aspirate. The technique achieves high cell purity and yield, making it suitable for diagnostic and basic research on human megakaryopoiesis.
Review
Cell Biology
M. Edward Quach
Summary: Platelet adhesion to vascular damage sites is a critical early step in hemostasis, with the GPIb-IX-V glycoprotein playing a key role in this process. In addition to aiding adhesion, GPIb-IX-V is crucial for platelet survival and regulation of clearance, working alongside multiple mechanisms to maintain a normal platelet count and ensure functional viability in circulation by removing aged or activated platelets. Dysregulation of platelet clearance, particularly through GPIb-IX-V, contributes to various bleeding disorders, including thrombocytopenia in conditions like VWD and immune thrombocytopenia.
Article
Biochemistry & Molecular Biology
Serena Barozzi, Valeria Bozzi, Daniela De Rocco, Tania Giangregorio, Patrizia Noris, Anna Savoia, Alessandro Pecci
Summary: This study identified a novel mutation affecting the cytoplasmic domain of GPIbβ, resulting in a mild form of BSS with a dominant effect causing macrothrombocytopenia in heterozygous individuals. The mutation does not affect assembly and trafficking of the GPIb-IX receptor, providing new insights into the pathophysiology of BSS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Hematology
Wenchun Chen, Moriah S. Wilson, Yingchun Wang, Wolfgang Bergmeier, Francois Lanza, Renhao Li
Summary: This study investigated the role of RAM.1 antibody in platelet clearance mediated by trigger sequence exposure. The results showed that RAM.1 can attenuate platelet clearance and prolong platelet lifespan, providing key evidence for the causative link between trigger sequence exposure and accelerated platelet clearance. This suggests the potential therapeutic development of a RAM.1 derivative for treating GPIb-IX-mediated thrombocytopenia.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Biochemistry & Molecular Biology
Ming Liu, Gan Wang, Runjia Xu, Chuanbin Shen, Heyu Ni, Ren Lai
Summary: Soy isoflavones genistein and daidzein were found to inhibit thrombosis formation, particularly under high shear force. They interact with 14-3-3ζ and suppress multiple platelet activation pathways and outside-in signaling transduction. These findings suggest that 14-3-3ζ is a novel target for genistein and daidzein in platelet inhibition.
Article
Hematology
Ingvild Jenssen Laegreid, Mats Irgen Olsen, Jon Inge Harr, Renathe Henriksen Gronli, Trude Victoria Mortberg, Siw Leiknes Ernstsen, Maria Therese Ahlen
Summary: This study describes a case of drug-induced immune thrombocytopenia (DITP) in a young adult male following dental treatment. The patient had two episodes of severe thrombocytopenia, which were found to be caused by the local anesthetic articaine. A bead-based Luminex platelet antigen assay proved useful in confirming the diagnosis of DITP. The study highlights the importance of thorough clinical evaluation and laboratory confirmation in suspected cases of DITP.
Article
Multidisciplinary Sciences
Chenjian Gu, Yang Wu, Huimin Guo, Yuanfei Zhu, Wei Xu, Yuyan Wang, Yu Zhou, Zhiping Sun, Xia Cai, Yutang Li, Jing Liu, Zhong Huang, Zhenghong Yuan, Rong Zhang, Qiang Deng, Di Qu, Youhua Xie
Summary: The study found that protoporphyrin IX and verteporfin are effective antiviral agents against SARS-CoV-2 infection, inhibiting the cytopathic effect and preventing viral binding to the receptor.
Article
Hematology
Danielle Karakas, Miao Xu, Heyu Ni
Summary: Thrombopoietin (TPO) plays important roles in platelet generation, with recent studies showing that platelets can induce TPO production in the liver in addition to clearing it. The platelet glycoprotein Ib alpha was found to be essential for platelet-mediated TPO generation.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Biochemistry & Molecular Biology
Anne K. Braczynski, Marc Sevenich, Ian Gering, Tatsiana Kupreichyk, Emil D. Agerschou, Yannick Kronimus, Pardes Habib, Matthias Stoldt, Dieter Willbold, Jorg B. Schulz, Jan-Philipp Bach, Bjorn H. Falkenburger, Wolfgang Hoyer
Summary: Parkinson's disease is associated with changes in the abundance of naturally occurring antibodies (nAbs) against alpha-synuclein (α Syn). In this study, nAbs-alpha Syn were found to specifically bind monomeric α Syn and inhibit its aggregation in vitro. Additionally, the addition of nAbs-alpha Syn to cultured cells affected intracellular α Syn aggregates.
Article
Hematology
Peter L. Turecek, Reinhard Ilk, Herbert Gritsch
Summary: This study evaluated how clinical diagnostic laboratories analyze recombinant VWF and plasma-derived VWF. The results showed that the analysis results for rVWF were consistent with the expected concentrations in some assays, while the analysis results for pdVWF underestimated its concentration in certain assays.
Article
Immunology
Andrey Mamontov, Igor Losev, Dmitrii Korzhevskii, Valeriia Guselnikova, Alexander Polevshchikov, Yulia Desheva
Summary: In this study, it was found that murine MCs can be activated to release histamine by introducing immune complexes, with a more significant effect on A/H5N1 influenza virus. The use of histamine receptor blockers can increase the survival rate of immunized mice.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Hematology
Theodore E. Warkentin, Maureen A. Smythe, Mona A. Ali, Naveed Aslam, Jo-Ann I. Sheppard, James W. Smith, Jane C. Moore, Donald M. Arnold, Ishac Nazy
Summary: IgG-specific and polyspecific PF4-dependent enzyme-immunoassays have high sensitivity for HIT diagnosis, but SRA+/EIA- HIT cases are exceptionally rare clinically. False-negative IgG-specific EIA results can lead to misdiagnosis, emphasizing the need for further clinical investigation and testing.
AMERICAN JOURNAL OF HEMATOLOGY
(2021)
Article
Veterinary Sciences
Marek Walczak, Malgorzata Juszkiewicz, Krzesimir Szymankiewicz, Anna Szczotka-Bochniarz, Grzegorz Wozniakowski
Summary: This study found that the anti-ASFV antibodies in serum samples from ASF survivors are not able to inhibit virus replication, suggesting that a broad spectrum of antibody and cellular immune response may play an important role in combating the virus in ASF survivors.
JOURNAL OF VETERINARY RESEARCH
(2022)
Article
Infectious Diseases
Motohiko Ogawa, Masayuki Shimojima, Masayuki Saijo, Masayoshi Fukasawa
Summary: Green tea-related polyphenols, especially EGCg and EGC, show potential as candidate anti-SFTSV drugs by inhibiting SFTSV infection in a concentration-dependent manner. Their antiviral activity is mainly achieved by reducing viral attachment to the host cells.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2021)
Article
Hematology
Muna Iraqi, Jose Perdomo, Feng Yan, Philip Y-I Choi, Beng H. Chong
Article
Cell Biology
Zohra Ahmadi, Jose Perdomo, Rose Wong, Beng H. Chong
Article
Multidisciplinary Sciences
Jose Perdomo, Feng Yan, Halina H. L. Leung, Beng H. Chong
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2017)
Article
Multidisciplinary Sciences
Jose Perdomo, Halina H. L. Leung, Zohra Ahmadi, Feng Yan, James J. H. Chong, Freda H. Passam, Beng H. Chong
NATURE COMMUNICATIONS
(2019)
Article
Hematology
Shiying Silvia Zheng, Jose Sail Perdomo, Halina Hoi Laam Leung, Feng Yan, Beng Hock Chong
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2020)
Editorial Material
Hematology
Jose Perdomo
Article
Pathology
Shiying Silvia Zheng, Jose Sail Perdomo, Zohra Ahmadi, Beng Hock Chong
Summary: Despite recent advances in understanding and treating immune thrombocytopenia (ITP), diagnosis remains clinical due to the lack of sensitive laboratory tests. Detection of anti-platelet antibodies (APA) in plasma, although highly specific, is notoriously insensitive. By evaluating multiple dilutions of plasma from 61 ITP patients, researchers found that using just two dilutions (1:2 and 1:25) could detect over 90% of patients with detectable plasma APA, improving the diagnostic value of indirect APA assay for ITP.
Article
Pathology
Zorik Chilingaryan, Tejas Deshmukh, Halina H. L. Leung, Jose Perdomo, Peter Emerson, Rahul Kurup, Beng H. Chong, James J. H. Chong
Summary: A study on arterial thrombi in acute myocardial infarction revealed the involvement of erythrocytes, neutrophil extracellular traps (NETs), and platelets, providing insights into the mechanisms of coronary artery thrombosis.
Article
Hematology
Halina H. L. Leung, Jose Perdomo, Zohra Ahmadi, Feng Yan, Steven E. McKenzie, Beng H. Chong
Summary: Research has shown that reactive oxygen species (ROS) and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 2 (NOX2) play crucial roles in thrombus formation in heparin-induced thrombocytopenia (HIT) by activating neutrophils to produce neutrophil extracellular traps (NETs) and induce thrombosis. Inhibiting ROS and NOX2 may serve as a potential new therapeutic target for treating HIT by blocking HIT-induced NETosis and thrombosis.
Article
Cardiac & Cardiovascular Systems
N. Fairooj Rashid, E. Zoe Clayton, Masahito Ogawa, Jose Perdomo, D. Robert Hume, Eddy Kizana, J. H. James Chong
Summary: Overexpression of Pdgf-a in the post-MI heart can modulate scar composition and improve left ventricular function, highlighting the potential of rAAV gene transfer of Pdgf-a as a cardio-reparative therapy.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2021)
Article
Hematology
Shiying Silvia Zheng, Zohra Ahmadi, Halina Hoi Laam Leung, Rose Wong, Feng Yan, Jose Sail Perdomo, Beng Hock Chong
Summary: Immune thrombocytopenia (ITP) is a bleeding disorder caused by dysregulated B- and T- cell functions, which lead to platelet destruction. This study found that specific anti-platelet antibodies are associated with different mechanisms of thrombocytopenia, and confirmed that the NEU1 inhibitor oseltamivir can prevent platelet destruction induced by anti-GPIIb/IIIa antibodies.
Article
Multidisciplinary Sciences
Halina H. L. Leung, Jose Perdomo, Zohra Ahmadi, Shiying S. Zheng, Fairooj N. Rashid, Anoop Enjeti, Stephen B. Ting, James J. H. Chong, Beng H. Chong
Summary: Anti-PF4 antibodies activate platelets and neutrophils, leading to thrombosis in vaccine-induced immune thrombotic thrombocytopenia (VITT). Inhibition of Fc gamma RIIa or NETosis can prevent thrombus formation. These findings provide insights into the pathogenesis of VITT and offer potential therapeutic targets.
NATURE COMMUNICATIONS
(2022)
Meeting Abstract
Hematology
Jose Perdomo, Halina H. L. Leung, Zohra Ahmadi, Yan Feng, Freda H. Passam, Beng H. Chong
Meeting Abstract
Hematology
Philip Young-Ill Choi, Kumiko Tanaka, Jose Perdomo, Beng H. Chong
Review
Immunology
Jose Perdomo
IMMUNOTARGETS AND THERAPY
(2016)