Article
Biochemistry & Molecular Biology
Giles E. Duffield, Maricela Robles-Murguia, Tim Y. Hou, Kathleen A. McDonald
Summary: The disruption of Id4 gene resulted in mice with abnormal circadian clock characteristics, such as shorter circadian period length and advanced phase angle of entrainment under different lighting conditions. Anatomical analysis revealed smaller suprachiasmatic nucleus (SCN) in Id4-/- mice, suggesting a possible influence on retinal afferents. The absence of ID4 protein may lead to changes in interactions with other Id gene family members, resulting in abnormal properties of the circadian clock.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Min Wang, Yuqian Wang, Mengdi Zhang, Qiqi Duan, Caifeng Chen, Qiong Sun, Meng Liu, Yan Zheng, Yongping Shao
Summary: KYNU is upregulated in psoriatic skin samples and plays a crucial role in the production of inflammatory factors and the development of psoriasis-like skin inflammation. The microRNA miR-203a can inversely regulate KYNU and reduce psoriasis-related inflammatory factors. KYNU inhibitors can alleviate pathological phenotypes in IMQ-mice.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Medicine, Research & Experimental
Julia C. Stokes, Rebecca L. Bornstein, Katerina James, Kyung Yeon Park, Kira A. Spencer, Katie Vo, John C. Snell, Brittany M. Johnson, Philip G. Morgan, Margaret M. Sedensky, Nathan A. Baertsch, Simon C. Johnson
Summary: Leukocyte proliferation plays a crucial role in the pathogenesis of Leigh syndrome, and inhibiting leukocytes can improve disease symptoms and prolong patient survival. This study has important implications for understanding mitochondrial disease mechanisms and developing new therapeutic strategies.
Article
Oncology
Maria Schubert, Franz Josef Gassner, Michael Huemer, Jan Philip Hoepner, Ekaterina Akimova, Markus Steiner, Alexander Egle, Richard Greil, Nadja Zaborsky, Roland Geisberger
Summary: The study demonstrates that AID contributes to the acquisition of somatic cancer-specific mutations in chronic lymphocytic leukemia, leading to accelerated disease progression and affecting the survival rate of mice. Therefore, further research on the impact of AID inhibition on drug resistance development is crucial.
Article
Microbiology
Yizhao Luan, Zehua Tang, Yao He, Zhi Xie
Summary: In this study, evidence was provided showing that coevolving residues in TF domains contribute to DNA binding specificity. It was demonstrated that the coevolving residues are more likely to coevolve with other TF subclass-determining sites and mutation of these coevolving residues could significantly reduce the stability of the TF-DNA complex. Overall, this study expands our understanding of the interaction among coevolving residues in TFs and their importance in transcriptional regulation.
MICROBIOLOGY SPECTRUM
(2023)
Review
Hematology
Elisa ten Hacken, Catherine J. Wu
Summary: Rapid advances in large-scale sequencing studies have revealed the genetic heterogeneity of CLL, while mouse models have become powerful tools for studying B-cell biology and new therapeutics. Genetic engineering technologies have enabled the creation of novel mouse models that provide deeper insights into disease evolution and therapy responses, contributing valuable platforms for functional genomic analyses and treatment studies in CLL and RS.
Article
Biochemistry & Molecular Biology
Dawei Zhou, Zhenyu Wu, Jun-Gyu Park, Guillaume N. Fiches, Tai-Wei Li, Qin Ma, Huachao Huang, Ayan Biswas, Luis Martinez-Sobrido, Netty G. Santoso, Jian Zhu
Summary: The study revealed a new role of the FACT complex in coordinating with BRD4 to regulate IFN signaling in both epithelial and NK cells, and proposed a novel application of the FACT inhibitor CBL0137 for treating viral infections.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Haruna Tani, Kaori Ishikawa, Hiroaki Tamashiro, Emi Ogasawara, Takehiro Yasukawa, Shigeru Matsuda, Akinori Shimizu, Dongchon Kang, Jun-Ichi Hayashi, Fan-Yan Wei, Kazuto Nakada
Summary: This study established a mouse model carrying a pathogenic mutation in the mitochondrial tRNA gene, associated with metabolic syndrome-like symptoms. The mutation led to decreased protein levels and reduced Complex I activity in mitochondria, providing insights into the molecular basis of mutation-mediated mitochondrial disorders.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Medicine, Research & Experimental
Zhonghua Lu, Yifei Yun, Yutong Zhang, Yao Ou, Meihua Wang
Summary: HDAC4 plays a key role in mediating radioresistance in esophageal carcinoma (EC) through downregulating miR-146a, which leads to suppressed proliferation and enhanced apoptosis of radioresistant EC cells. Loss of HDAC4 can sensitize EC cells to radiotherapy both in vitro and in vivo. This study contributes to a better understanding of the mechanism underlying radioresistance in EC.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Neurosciences
Ali Jahanbazi Jahan-Abad, Hannah E. Salapa, Cole D. Libner, Patricia A. Thibault, Michael C. Levin
Summary: This study reveals the dysfunction of RNA binding protein hnRNP A1 in oligodendrocytes (OLs) in patients with multiple sclerosis (MS) as well as in an animal model of MS. The dysfunction of hnRNP A1 is associated with alterations in RNA metabolism, cell morphology, and programmed cell death pathways in OLs, leading to detrimental effects on OL functioning and survival. These findings suggest a potential mechanism of OL damage and death in MS.
Article
Oncology
Maxine Umeh-Garcia, Henriette O'Geen, Catalina Simion, Melanie Hayden Gephart, David J. Segal, Colleen A. Sweeney
Summary: This study reveals the association between DNA methylation and the silencing of the LRIG1 gene in basal/triple-negative breast cancer. The global demethylating agent 5-aza-2'-deoxycytidine decreases methylation and increases LRIG1 expression. Targeted demethylation and transcriptional activation using CRISPR/deadCas9 technology significantly enhances LRIG1 expression and reduces cancer cell viability.
BRITISH JOURNAL OF CANCER
(2022)
Article
Medicine, Research & Experimental
Jieyu Wang, Fangfang Zhong, Jun Li, Huiran Yue, Wenzhi Li, Xin Lu
Summary: CHD4 plays an important role in the metastasis of ovarian cancer cells. High CHD4 expression is associated with a poor prognosis in ovarian cancer patients. Inhibition of CHD4 function suppresses the migration and invasion of ovarian cancer cells by downregulating EZH2 and nuclear beta-catenin. Romidepsin, an HDAC1/2 inhibitor, shows potential therapeutic effects on inhibiting CHD4-mediated metastasis.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Ryonosuke Taniguchi, Koichi Utani, Bhushan Thakur, Kazuho Ishine, Mirit Aladjem, Noriaki Shimizu
Summary: SIRT1 is involved in the regulation of gene amplification, and its depletion affects gene amplification and expression. The study of the expression of amplified repetitive DNA genes in SIRT1-deficient cells suggests that SIRT1 may regulate gene expression.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Virology
Bharati Singh, Kiran Avula, Shamim Akhtar Sufi, Nahid Parwin, Sayani Das, Mohd Faraz Alam, Subhashish Samantaray, Leelakrishna Bankapalli, Alankrita Rani, Kokavalla Poornima, Biswajita Prusty, Tareni P. Mallick, Shubham K. Shaw, Hiren Dodia, Shobhitendu Kabi, Trupti T. Pagad, Sriprasad Mohanty, Gulam Hussain Syed
Summary: Mitochondrial homeostasis is disrupted in dengue-infected liver cells, leading to impaired mitochondrial quality control and biogenesis pathways, cellular injury, and necrotic death. Dengue infection suppresses mitophagy, disrupts mitochondrial biogenesis, and releases mtDAMPs, triggering proinflammatory signaling in immune cells.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Marlena Rademacher, Hartmut Kuhn, Astrid Borchert
Summary: GPX4, as the only enzyme capable of reducing complex lipid peroxides to the corresponding alcohols, plays a role in erythroid differentiation of mouse erythroleukemia cells, but heterozygous expression of a catalytically inactive Gpx4 is not sufficient to compromise erythropoiesis in vivo and ex vivo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Christoph Niemoeller, Julius Wehrle, Julian Riba, Rainer Claus, Nathalie Renz, Janika Rhein, Sabine Bleul, Juliane M. Stosch, Justus Duyster, Christoph Plass, Pavlo Lutsik, Daniel B. Lipka, Michael Lubbert, Heiko Becker
Summary: The study introduces a novel method, epi-gSCAR, for analyzing DNA methylation and genetic variants in single cells, allowing for accurate and reproducible measurements at the single cell level, and enabling differentiation between cell lines based on their DNA methylation and genetic profiles.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemical Research Methods
Phillip Loehr, Stefan Schiele, Tim Tobias Arndt, Stefanie Gruetzner, Rainer Claus, Christoph Roemmele, Gernot Mueller, Christoph Schmid, Kevin M. Dennehy, Andreas Rank
Summary: This study analyzed the changes in lymphocyte subsets in COVID-19 patients and found that cell counts of lymphocytes and their subsets are reduced in severe cases. Age and gender also play a role in the altered cell counts.
Letter
Hematology
Patrick Mayr, Tatiana Gruenewald, Giuliano Filippini Velazquez, Andreas Rank, Christoph Schmid, Manuela Harloff, Bruno Maerkl, Martin Trepel, Klaus Hirschbuehl, Tim Pfeiffer, Rainer Claus
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Article
Oncology
Johanna Waidhauser, Pia Nerlinger, Tim Tobias Arndt, Stefan Schiele, Florian Sommer, Sebastian Wolf, Phillip Loehr, Stefan Eser, Gernot Mueller, Rainer Claus, Bruno Maerkl, Andreas Rank
Summary: Significant changes in circulating lymphocyte subsets were found in colon carcinoma patients, independent of physiological alterations due to gender or age. Some lymphocyte subsets showed significant differences according to tumor localization or MSI-status.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2022)
Article
Oncology
Frank Jordan, Martin Trepel, Rainer Claus
Summary: This case report highlights the potential option of re-exposure to Ipilimumab as a strategy to restore immune-mediated disease control in patients with MSI-H mCRC who have had a long-lasting response to Ipilimumab/Nivolumab.
CLINICAL COLORECTAL CANCER
(2022)
Letter
Allergy
Tina Schaller, Bruno Maerkl, Rainer Claus, Lynette Sholl, Jason L. Hornick, Matthew P. Giannetti, Lisa Schweizer, Matthias Mann, Mariana Castells
Article
Pathology
Klaus Hirschbuehl, Tina Schaller, Bruno Maerkl, Rainer Claus, Eva Sipos, Lukas Rentschler, Andrea Maccagno, Bianca Grosser, Elisabeth Kling, Michael Neidig, Thomas Kroncke, Oliver Spring, Georg Braun, Hans Boesmueller, Maximilian Seidl, Irene Esposito, Jessica Pablik, Julia Hilsenbeck, Peter Boor, Martin Beer, Sebastian Dintner, Claudia Wylezich
Summary: This study aimed to analyze the causes of death, histological organ alteration, and viral spread in individuals who died after vaccination and had proven SARS-CoV-2 infection. The study found that the rate of viral dissemination was significantly higher in vaccinated cases, and vaccinated individuals also had high viral loads and immunosuppressive factors. Partially vaccinated patients were more affected than fully vaccinated individuals. Future studies should further investigate the potential role of antibody-dependent enhancement.
Article
Oncology
Patrick Mayr, Mathias Lutz, Maximilian Schmutz, Jens Hoeppner, Friederike Liesche-Starnecker, Juergen Schlegel, Jochen Gaedcke, Rainer Claus
Summary: JC virus reactivation causing progressive multifocal leukoencephalopathy (PML) is rare in patients with solid tumors, and this case report describes a patient who developed persistent and progressive leukopenia and lymphopenia resulting in death after PML onset.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Florian Lueke, Florian Haller, Kirsten Utpatel, Markus Krebs, Norbert Meidenbauer, Alexander Scheiter, Silvia Spoerl, Daniel Heudobler, Daniela Sparrer, Ulrich Kaiser, Felix Keil, Christoph Schubart, Lars Toegel, Sabine Einhell, Wolfgang Dietmaier, Ralf Huss, Sebastian Dintner, Sebastian Sommer, Frank Jordan, Maria-Elisabeth Goebeler, Michaela Metz, Diana Haake, Mithun Scheytt, Elena Gerhard-Hartmann, Katja Maurus, Stephanie Brandlein, Andreas Rosenwald, Arndt Hartmann, Bruno Maerkl, Hermann Einsele, Andreas Mackensen, Wolfgang Herr, Volker Kunzmann, Ralf Bargou, Matthias W. Beckmann, Tobias Pukrop, Martin Trepel, Matthias Evert, Rainer Claus, Alexander Kerscher
Summary: Molecular Tumor Boards (MTBs) are crucial for discussing personalized therapies for cancer patients. However, there is limited evidence on the regional impact and access to MTBs. A study in Germany analyzed MTB patient data from four neighboring cancer centers and identified regional differences and underrepresented areas. These findings help address inequalities in patient access to precision oncology.
Editorial Material
Radiology, Nuclear Medicine & Medical Imaging
Johanna S. S. Enke, Nic G. G. Reitsam, Tina Schaller, Rainer Claus, Constantin Lapa, Alexander Dierks
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2023)
Article
Medicine, General & Internal
Barbara Eichhorst, Carsten U. Niemann, Arnon P. Kater, Moritz Fuerstenau, Julia von Tresckow, Can Zhang, Sandra Robrecht, Michael Gregor, Gunnar Juliusson, Patrick Thornton, Philipp B. Staber, Tamar Tadmor, Vesa Lindstrom, Caspar da Cunha-Bang, Christof Schneider, Christian B. Poulsen, Thomas Illmer, Bjoern Schoettker, Thomas Noesslinger, Ann Janssens, Ilse Christiansen, Michael Baumann, Henrik Frederiksen, Marjolein van der Klift, Ulrich Jaeger, Maria B. L. Leys, Mels Hoogendoorn, Kourosh Lotfi, Holger Hebart, Tobias Gaska, Harry Koene, Lisbeth Enggaard, Jereon Goede, Josien C. Regelink, Anouk Widmer, Florian Simon, Nisha De Silva, Anna-Maria Fink, Jasmin Bahlo, Kirsten Fischer, Clemens-Martin Wendtner, Karl A. Kreuzer, Matthias Ritgen, Monika Brueggemann, Eugen Tausch, Mark-David Levin, Marinus van Oers, Christian Geisler, Stephan Stilgenbauer, Michael Hallek
Summary: In fit patients with CLL, venetoclax-obinutuzumab, with or without ibrutinib, showed superior results compared to chemoimmunotherapy as a first-line treatment, with higher rates of undetectable minimal residual disease and progression-free survival.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Oncology
Luise Victoria Claass, Patrick Mayr, Lisa Paschold, Thomas Weber, Denis Terziev, Bertram Jehs, Richard Brill, Johannes Dober, Bruno Maerkl, Claudia Wickenhauser, Piotr Czapiewski, Martin Trepel, Rainer Claus, Mascha Binder
Summary: SARS-CoV-2 vaccines do not seem to be associated with the development of lymphomas in the lymph nodes of the deltoid region used for vaccination. The study found that cases of unilateral axillary lymphomas occurred both before and after the introduction of SARS-CoV-2 vaccines, and there was no evidence of a correlation between the vaccines and the malignant clonotypes.
Article
Medicine, Research & Experimental
Lisa Schweizer, Tina Schaller, Maximilian Zwiebel, Oezge Karayel, Johannes Bruno Mueller-Reif, Wen-Feng Zeng, Sebastian Dintner, Thierry M. Nordmann, Klaus Hirschbuehl, Bruno Maerkl, Rainer Claus, Matthias Mann
Summary: SARS-CoV-2 can cause damage to lung tissue and other organs in the human body, and this study aimed to analyze these effects comprehensively. Using a mass spectrometry proteomics workflow, the researchers identified inflammatory responses as the initial reaction in all tissues. They also found specific patterns of damage in different organs, such as diffuse alveolar damage in the lungs and organ-specific changes in the kidneys, liver, and lymphatic and vascular systems. In the brain, secondary inflammatory effects were linked to neurotransmitter receptors and myelin degradation. These findings contribute to our understanding of the mechanisms of COVID-19 and provide insights for organ-specific therapeutic interventions.
EMBO MOLECULAR MEDICINE
(2023)
Article
Health Care Sciences & Services
Saskia von Stillfried, Roman David Buelow, Rainer Roehrig, Peter Boor
Summary: The German COVID-19 Autopsy Registry (DeRegCOVID) serves as a central hub for multicenter autopsy-based studies and provides support for researchers and data analysis. The registry shows that COVID-19 is the underlying cause of death in most autopsy cases, with diffuse alveolar damage and multi-organ failure being the most common immediate causes of death. The registry is of great importance for medical research, policy-making, and public discussion.
LANCET REGIONAL HEALTH-EUROPE
(2022)
Article
Critical Care Medicine
Saskia von Stillfried, Roman David Buelow, Rainer Roehrig, Patrick Meybohm, Peter Boor
Summary: This study analyzed data from the German COVID-19 autopsy registry and found that bleeding events were more frequent in ECMO cases, with a higher proportion of intracranial bleeding. The study suggests the potential value of autopsies and a joint interdisciplinary multicenter approach in addressing fatal complications in COVID-19.