Article
Chemistry, Multidisciplinary
Liang Ding, Wenjing Zhou, Jian Zhang, Qingsong Tang, Weizhang Xiao, Ming Chen, Zhenya Shen, Weiqian Chen
Summary: Uncontrolled inflammation can cause sepsis-associated immune disorders, and EVs can be used as drug carriers to target inflammatory monocytes for treatment, showing potential clinical significance.
Article
Immunology
Benjamin G. Korte, Morgan A. Giese, Gayathri Ramakrishnan, Stella Ma, David Bennin, Julie Rindy, Colin N. Dewey, Anna Huttenlocher
Summary: Neutrophils in the tumor microenvironment undergo altered functions. This study used TRAP and RNA sequencing to identify changes in neutrophil, macrophage, and transformed epithelial cell transcription induced by oncogenic Ras in larval zebrafish. It was found that transformed epithelial cells and neutrophils exhibited significant changes in gene expression, while macrophages were unaffected. Neutrophils had more down-regulated genes, whereas gene expression was primarily upregulated in transformed epithelial cells. The antioxidant thioredoxin was upregulated in response to oncogenic Ras and played a role in tumor initiation. The findings suggest that changes in neutrophil gene expression and behavior during tumor initiation can influence the proliferation of transformed cells in the early tumor microenvironment.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Chan Xiang, Min Zhang, Zhanxian Shang, Shengnan Chen, Jikai Zhao, Bowen Ding, Dong Jiang, Qian Zhu, Haohua Teng, Lei Zhu, Jinchen Shao, Ruiying Zhao, Min Ye, Yang Yu, Yuchen Han
Summary: This study reveals the cellular mechanism underlying the formation of the immunosuppressive microenvironment in lung adenocarcinoma (LUAD) through single-cell transcriptome analysis. The study identified increased expression of tumor-associated macrophage-folate receptor 2 (TAM-FOLR2) and CD4(+) nuclear receptor subfamily 4 group A member 3 (NR4A3) in invasive adenocarcinoma (IA). Additionally, the study suggests a potential trajectory of alveolar resident macrophage-resistin (ARM-RETN)/TAM-FOLR2/dendritic cells (DCs)/CD4(+)NR4A3/CD4(+)FOXP3 in shaping the immune suppressive microenvironment during the pathogenesis of LUAD.
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Yuen Lam Dora Ng, Evelyn Ramberger, Stephan R. Bohl, Anna Dolnik, Christian Steinebach, Theresia Conrad, Sina Mueller, Oliver Popp, Miriam Kull, Mohamed Haji, Michael Guetschow, Hartmut Doehner, Wolfgang Walther, Ulrich Keller, Lars Bullinger, Philipp Mertins, Jan Kroenke
Summary: The study identifies CDK6 upregulation as a druggable target in IMiD-resistant multiple myeloma through integrated proteomic, phosphoproteomic, and RNA sequencing analysis.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Nan Xiang, Hao Xu, Zhou Zhou, Junyu Wang, Pengfei Cai, Li Wang, Zhen Tan, Yingbo Zhou, Tianping Zhang, Jiayuan Zhou, Ke Liu, Songwen Luo, Minghao Fang, Guosheng Wang, Zhuo Chen, Chuang Guo, Xiaomei Li
Summary: This study used single-cell RNA sequencing to investigate cellular heterogeneity in patients with primary Sjogren's syndrome (pSS). The findings suggest that downregulated SOX9 in myoepithelial cells might be associated with impaired epithelial regeneration in pSS. Additionally, an expanded ACKR1(+) endothelial population in pSS patients may play a role in lymphocyte migration. The study also identified expanded IGHD(+) naive B cells in the peripheral blood of pSS patients.
Article
Multidisciplinary Sciences
Carlos Aleman-Ruiz, Wenyi Wang, Ray Dingledine, Nicholas H. Varvel
Summary: The study demonstrates that blocking the brain entry of monocytes through CCR2 can alleviate the adverse effects of generalized status epilepticus, including blood-brain barrier erosion, microgliosis, and neuronal damage, while enhancing weight regain.
SCIENTIFIC REPORTS
(2023)
Article
Neurosciences
Juliet Goldsmith, Alban Ordureau, J. Wade Harper, Erika L. F. Holzbaur
Summary: This study reveals the importance of autophagy in maintaining neuronal health. By isolating autophagic vesicles and using proteomics, the researchers identified mitochondrial proteins as a major cargo engulfed in autophagosomes. They also found that nucleoid-associated proteins are enriched in autophagic engulfment of nucleoid-enriched mitochondrial fragments, and this process requires the mitochondrial fission machinery protein Drp1.
Article
Dermatology
Wei-Che C. Ko, Li Li, Taylor R. Young, Riley E. McLean-Mandell, April C. Deng, Vijay K. Vanguri, Karen Dresser, John E. Harris
Summary: Subacute cutaneous lupus erythematosus and chronic cutaneous lupus erythematosus are the main subtypes of cutaneous lupus, with distinct differences in dermatological examination but similarities in gene expression when compared to each other. Both subtypes show upregulation of IFN-1 and T-cell chemotactic genes, suggesting inflammatory components in skin lupus pathogenesis, while lupus nephritis displays recruitment and activation of M2 macrophages and a wound healing signature.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Immunology
Yuehan Gao, He Li, Zhaohuai Li, Lihui Xie, Xiuxing Liu, Zhaohao Huang, Binyao Chen, Xianchai Lin, Xianggui Wang, Yingfeng Zheng, Wenru Su
Summary: The study identified different subtypes within human moDCs and blood DCs, revealing their transcriptomic differences and differentiation trajectories, which will enable more accurate functional and developmental analyses of human cDC2s and moDCs.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Genetics & Heredity
Salman M. Toor, Sachin Wani, Omar M. E. Albagha
Summary: Osteoclasts are the only bone resorbing cells and play an important role in skeletal mass and structure. Differentiation of osteoclasts involves a complex regulatory network of genes and LncRNAs, with potential implications for understanding bone-related diseases.
FRONTIERS IN GENETICS
(2021)
Article
Cell Biology
Ozge Karayel, Sebastian Virreira Winter, Shalini Padmanabhan, Yuliya Kuras, Duc Tung Vu, Idil Tuncali, Kalpana Merchant, Anne-Marie Wills, Clemens R. Scherzer, Matthias Mann
Summary: The study presents a mass spectrometry-based workflow for analyzing the CSF proteome of Parkinson's disease (PD) patients. The workflow identifies several proteins that are altered in PD patients or significantly correlated with clinical scores, and reveals enhanced neuroinflammation in LRRK2 G2019S carriers. Comparison with urinary proteomes also demonstrates overlapping PD-associated changes, including lysosomal protein alterations, shedding light on the understanding of PD pathogenesis.
CELL REPORTS MEDICINE
(2022)
Article
Hematology
Aisha Jibril, Charlotte Hellmich, Edyta E. Wojtowicz, Katherine Hampton, Rebecca Maynard, Ravindu De Silva, Dominic J. Fowler-Shorten, Jayna J. Mistry, Jamie A. Moore, Kristian M. Bowles, Stuart A. Rushworth
Summary: Cell-free mtDNA is elevated in the peripheral blood and BM of patients with myeloma. Myeloma-derived mtDAMPs remodel the BM microenvironment through STING activation. These findings provide insights into the mechanism and functional consequences of mtDAMPs in myeloma disease progression.
Article
Immunology
Masa Filipovic, Darja Flegar, Sara Anicic, Dino Sisl, Tomislav Kelava, Natasa Kovacic, Alan Sucur, Danka Grcevic
Summary: This study identified differentially expressed genes in different subsets of osteoclast progenitor cells (OCPs) associated with arthritis, and these genes may help identify the characteristics of arthritis and indicate potential therapeutic targets for modulating osteoclast activity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Sandeep Keshavan, Fernando Torres Andon, Audrey Gallud, Wei Chen, Knut Reinert, Lang Tran, Bengt Fadeel
Summary: Engineered nanomaterials have potential risks to human health, and studies have shown that multi-walled carbon nanotubes (MWCNTs) significantly affect chemokine signaling pathways. Evaluating sub-lethal concentrations of nanomaterials in relevant target cells is crucial.
Article
Multidisciplinary Sciences
David Ohayon, Marion Aguirrebengoa, Nathalie Escalas, Thomas Jungas, Cathy Soula
Summary: In this study, a unique subtype of spinal astrocytes known as Olig2-AS was identified, with 245 differentially expressed genes, 135 of which showed higher expression levels compared to other spinal astrocyte populations, serving as a "unique" functional signature of Olig2-AS. Additionally, two genes (inka2 and kcnip3) were identified as specific molecular markers of Olig2-AS in the P7 spinal cord.
Article
Oncology
Julie Devin, Tatiana Caneque, Yea-Lih Lin, Lucie Mondoulet, Jean-Luc Veyrune, Matthieu Abouladze, Elvira Garcia De Paco, Ouissem Karmous Gadacha, Guillaume Cartron, Philippe Pasero, Caroline Bret, Raphael Rodriguez, Jerome Moreaux
Summary: Diffuse large B-cell lymphoma (DLBCL) is a common hematological malignancy, and alterations in iron metabolism may be targeted for therapy. Iron score (IS), a gene expression-based risk score, can identify high-risk DLBCL patients who may benefit from targeting iron homeostasis. Ironomycin, a potential treatment for DLBCL, inhibits cell proliferation and induces cell death.
Review
Economics
Mimi Choon-Quinones, Dirk Hose, Zoltan Kalo, Tamas Zelei, Jean-Luc Harousseau, Brian Durie, Paul Keown, Mike Barnett, Ivett Jakab
Summary: Decision-aids (DAs) can assist in shared decision-making by providing evidence-based information for healthcare professionals, patients, and caregivers. However, current DAs lack a comprehensive framework of decision factors.
Article
Oncology
Hatice Satilmis, Emma Verheye, Philip Vlummens, Inge Oudaert, Niels Vandewalle, Rong Fan, Jennifer M. Knight, Nathan De Beule, Gamze Ates, Ann Massie, Jerome Moreaux, Anke Maes, Elke De Bruyne, Karin Vanderkerken, Eline Menu, Erica K. Sloan, Kim De Veirman
Summary: This study investigates the potential therapeutic effects of beta-blockers, specifically targeting the beta(2)-adrenergic receptor, in multiple myeloma treatment. The blockade of beta(2)-adrenergic receptors reduces cell viability, induces apoptosis and autophagy, and modulates cancer cell metabolism. Combining beta(2)AR blockade with other drugs enhances apoptosis in multiple myeloma cells.
JOURNAL OF PATHOLOGY
(2023)
Article
Oncology
Elias K. Mai, Stefanie Huhn, Kaya Miah, Alexandra M. Poos, Christof Scheid, Katja C. Weisel, Uta Bertsch, Markus Munder, Oscar Berlanga, Dirk Hose, Anja Seckinger, Anna Jauch, Igor W. Blau, Mathias Haenel, Hans J. Salwender, Axel Benner, Marc S. Raab, Hartmut Goldschmidt, Niels Weinhold
Summary: Mass spectrometry (MS) is a promising tool for monitoring monoclonal protein in plasma cell dyscrasias. Our study found that MS negativity was significantly associated with improved progression-free survival (PFS) in multiple myeloma patients, even in those patients with complete response (CR). Combining MS and baseline cytogenetics improved the prediction of outcome, and sequential MS combined with baseline disease features and minimal residual disease (MRD) can further improve its clinical value.
BLOOD CANCER JOURNAL
(2023)
Review
Economics
Mimi Choon-Quinones, Tamas Zelei, Bertalan Nemeth, Manna Toth, Xiao Yang Jia, Mike Barnett, Paul Keown, Brian Durie, Jean-Luc Harousseau, Dirk Hose, Zoltan Kalo
Summary: The aim of this study was to review economic evaluations of health technologies in multiple myeloma (MM) and provide recommendations for future analyses. A systemic literature review was conducted and it was found that most economic analyses were from high-income countries, while evaluations from middle-income countries were rare. Diagnostic technologies and integrated care were rarely modelled, and there was a lack of evaluations regarding minimal residual disease (MRD).
JOURNAL OF MEDICAL ECONOMICS
(2023)
Article
Oncology
Rong Fan, Hatice Satilmis, Niels Vandewalle, Emma Verheye, Philip Vlummens, Anke Maes, Catharina Muylaert, Elke De Bruyne, Eline Menu, Holly Evans, Andrew Chantry, Nathan De Beule, Dirk Hose, Marie Toerngren, Helena Eriksson, Karin Vanderkerken, Ken Maes, Karine Breckpot, Kim De Veirman
Summary: This study reveals the dual therapeutic effects of the immunomodulator tasquinimod in multiple myeloma patients, including inhibition of tumor cell proliferation and promotion of immune activity. The results suggest that tasquinimod can inhibit immunosuppressive myeloid cells in the bone marrow by targeting S100A9, and enhance T cell activity and functionality.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Biochemistry & Molecular Biology
L. Baert, B. Manfroi, M. Quintero, O. Chavarria, P. V. Barbon, E. Clement, A. Zeller, T. Van Kuppevelt, N. Sturm, J. Moreaux, A. Tveita, B. Bogen, T. McKee, B. Huard
Summary: Multiple myeloma is a hematological neoplasm derived from plasma cells in the bone marrow. A subpopulation of cells called 10e4(+) cells, which have high resistance to multiple myeloma drugs, bind to the protein APRIL through heparan sulfate chains on syndecan-1. These cells have high proliferation activity and are able to form colonies in 3D cultures. They can develop in the bone marrow after intravenous injection and become more resistant to drugs after treatment. The enzyme HS3ST3a1 plays a role in modifying syndecan-1 to confer reactivity to 10e4 and APRIL binding. Targeting this enzyme could potentially improve drug resistance control in multiple myeloma.
Article
Genetics & Heredity
Mohammad Salma, Elina Alaterre, Jerome Moreaux, Eric Soler
Summary: Var vertical bar Decrypt is a web-based tool designed to extract relevant functional information from whole-exome sequencing (WES) data. It offers various filtering and analysis tools for prioritizing gene variants. By using Var vertical bar Decrypt, we successfully identified known disease oncogenes and novel putative drivers in WES datasets of acute erythroid leukemia patients.
EPIGENETICS & CHROMATIN
(2023)
Article
Hematology
Sarah Bonnet, Serge Carillo, Baptiste Legrand, Barbara Burroni, Thierry Lavabre-Bertrand, Guilhem Requirand, Nicolas Robert, Lea Fornero, Ahmed Al Mansoori, Jerome Moreaux, Guillaume Cartron, Ludovic Gabellier, Charles Herbaux
Summary: This case study describes a patient with extreme thrombocytosis who unfortunately had a rapidly fatal outcome. The cause of the thrombocytosis remained unknown and the patient did not show signs of myelofibrosis. However, dysplastic megakaryocytes were observed. The patient had a novel variant of the CALR gene in exon 3 (C105S), as well as mutations in ASXL1, U2AF1, and EZH2. These mutations were found in myeloid cells but not in lymphoid cells. The patient was diagnosed with a rare case of myelodysplastic syndrome/myeloproliferative neoplasm (MDS/MPN). Due to the high risk of thrombosis, the patient was treated with hydroxycarbamide. As the hematological status worsened, two new mutations, SETBP1 and ETV6, appeared, while the CALR mutation and the three other mutations identified in the chronic stage were still detectable. These findings suggest that the CALR variant may contribute to the pathogenesis of MDS/MPN in this patient.
EUROPEAN JOURNAL OF HAEMATOLOGY
(2023)
Letter
Oncology
Djamila Chemlal, Emmanuel Varlet, Amelie Machura, Sara Ovejero, Guilhem Requirand, Nicolas Robert, Guillaume Cartron, Elina Alaterre, Caroline Bret, Laure Vincent, Charles Herbaux, Giacomo Cavalli, Angelique Bruyer, Hugues De Boussac, Jerome Moreaux
Article
Medical Laboratory Technology
Emilia Boris, Alexandre Theron, Valentin Montagnon, Nicolas Rouquier, Marion Almeras, Jerome Moreaux, Caroline Bret
Summary: This study aimed to investigate the landscape of 7 leukemia-associated phenotype (LAP) markers in B acute lymphoblastic leukemia (B ALL). The expression levels of these markers in normal leukocytes, normal B cell differentiation, and B lymphoblasts at diagnosis of B ALL were evaluated. The study also examined the prognostic value of these markers using Maxstat R algorithm.
CYTOMETRY PART B-CLINICAL CYTOMETRY
(2023)
Meeting Abstract
Oncology
Catharina Muylaert, Lien Ann Van Hemelrijck, Elina Alaterre, Nicolas Robert, Guilhem Requirand, Kim De Veirman, Eline Menu, Karin Vanderkerken, Jerome Moreaux, Elke De Bruyne
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2023)
Meeting Abstract
Oncology
Inge Oudaert, Judith Lind, Osman Aksoy, Catharina Muylaert, Hatice Satilmis, Sylvia Faict, Jerome Moreaux, Kim De Veirman, Elke De Bruyne, Sonia Vallet, Karin Vanderkerken, Klaus Podar, Eline Menu
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2023)
Meeting Abstract
Oncology
Sara Ovejero, Julie Devin, Tatiana Caneque, Laura Henry, Laura Alibert, Guilhem Requirand, Nicolas Robert, Alizee Steer, Amelie Machura, Christophe Hirtz, Angelique Bruyer, Laure Vincent, Guillaume Cartron, Charles Herbaux, Raphael Rodriguez, Caroline Bret, Jerome Moreaux
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2023)
Meeting Abstract
Oncology
Laure Dutrieux, Guillemin Antoine, Lin Yea-Lih, Malik Lutzmann, Guilhem Requirand, Nicolas Robert, Laure, Guillaume Cartron, Charles Herbaux, Raphael Rodriguez, Michel Cogne, Philippe Pasero, Jerome Moreaux
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2023)
