Article
Biochemical Research Methods
Erin E. Berlew, Keisuke Yamada, Ivan A. Kuznetsov, Eleanor A. Rand, Chandler C. Ochs, Zaynab Jaber, Kevin H. Gardner, Brian Y. Chow
Summary: In this study, we efficiently created single-component optogenetic tools for membrane recruitment-based signaling perturbation using BcLOV4 technology. Screening a limited set of genetic constructs and observing their dynamic translocation in response to light enabled the identification of viable signaling control tools. We successfully created an optogenetic Cdc42 GTPase and Rac1-activating Tiam1 GEF protein, completing a toolbox for spatiotemporally precise induction of Rho-family GTPase signaling.
ACS SYNTHETIC BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Kana Asano-Matsuda, Sajida Ibrahim, Tomoko Takano, Jun Matsuda
Summary: The initial step of urine formation involves selective filtration of plasma in the glomerulus, where a filtration barrier made up of three layers (fenestrated endothelial cells, glomerular basement membrane, and podocytes) retains blood cells and large proteins while removing waste products. Rho GTPases serve as crucial molecular switches in regulating podocyte health and barrier function, with particular focus on subcellular compartments where signaling events occur.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Duoyan Rong, Shuai Zhao, Wenxin Tang, Nan Luo, Hai He, Zhuqing Wang, Huimin Ma, Yimei Huang, Xiaoxun Yao, Xue Pan, Lin Lv, Jiajing Xiao, Renyi Liu, Shingo Nagawa, Chizuko Yamamuro
Summary: Cell polarity is crucial for plant development, and the ROP gene plays a key role in regulating cell polarity. The study found that M. polymorpha is an excellent genetic system for studying ROP signaling pathways. The results demonstrate the critical function of MpROP in plant development regulation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Ravinder Kandi, Katharina Senger, Ani Grigoryan, Karin Soller, Vadim Sakk, Tanja Schuster, Karina Eiwen, Manoj B. Menon, Matthias Gaestel, Yi Zheng, Maria Carolina Florian, Hartmut Geiger
Summary: The Cdc42-Borg4-Septin7 axis is crucial for maintaining polarity and function in HSCs, with genetic deletion of Borg4 or Septin7 resulting in reduced polarity frequency and engraftment potential of HSCs.
Article
Biochemistry & Molecular Biology
Mutsuko Kukimoto-Niino, Kentaro Ihara, Chiemi Mishima-Tsumagari, Mio Inoue, Yoshinori Fukui, Shigeyuki Yokoyama, Mikako Shirouzu
Summary: This study reveals the crystal structures of the catalytic DHR2 domain of mouse DOCK10, complexed with either Cdc42 or Rac1, and uncovers the unique dual activation mechanism of DOCK10.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Review
Cell Biology
Klaus Ebnet, Volker Gerke
Summary: Membrane polarity, regulated by polarized membrane trafficking and the presence of intramembrane diffusion barriers, is crucial for the development of multicellular tissues. Rho and Rab family small GTPases play important roles in cell-cell junction formation and polarized membrane traffic.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Immunology
Heidi Tackenberg, Sonja Moeller, Marie-Dominique Filippi, Tamas Laskay
Summary: This study demonstrates a negative regulatory role of the small GTPase Cdc42 in Neutrophil Extracellular Trap formation in primary neutrophils, with a mechanism different from known NETosis pathways, involving activation of SK channels and mitochondria. This finding suggests Cdc42 activity as a novel target for modulating the formation of Neutrophil Extracellular Traps.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Neurosciences
Maxsam S. Donta, Yogesh Srivastava, Pierre D. McCrea
Summary: This study explores how delta-catenin modulates small Rho GTPase activity. The regulation of small Rho GTPase activity is crucial for normal learning and memory as well as synapse formation, while dysregulation of delta-catenin is associated with various diseases such as cancer and AD.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Neurosciences
Noelle C. Punessen, Claudia Pena, Alexandra Sandberg, Lilia A. Koza, Daniel A. Linseman
Summary: Neurodegenerative diseases are characterized by the progressive destruction of neurons in the brain and spinal cord. Current treatments mainly focus on alleviating symptoms rather than targeting the underlying mechanisms of these diseases. This study investigates the role of Cdc42, a member of the Rho GTPase family, and its downstream effectors in neuronal survival and apoptosis. The results suggest that Cdc42/ACK-1 signaling plays a key role in regulating neuronal susceptibility to cell death, particularly in neurodegenerative disorders.
MOLECULAR AND CELLULAR NEUROSCIENCE
(2023)
Review
Cell Biology
Qiaojuan Mei, Huiying Li, Yu Liu, Xiaofei Wang, Wenpei Xiang
Summary: CDC42, a member of the Rho-GTPase family, plays a crucial role in establishing polarity and ensuring proper gametogenesis in female vertebrate oocytes. Through its local occupation of specific regions and synergistic regulation in a multilevel signalling network, CDC42 ensures precise assembly of the contractile ring at the right time and place for accurate gametogenesis.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Liad Segal, Sharon Etzion, Sigal Elyagon, Moran Shahar, Hadar Klapper-Goldstein, Aviva Levitas, Michael S. Kapiloff, Ruti Parvari, Yoram Etzion
Summary: Dock10 is a critical factor that regulates the JNK and p38 MAPK signaling cascades in the myocardium. Experimental evidence from cell studies and mouse models suggests that Dock10 plays an important role in inhibiting cardiac stress response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Vanessa Aline Bernusso, Karla P. Vieira, Adriana S. S. Duarte, Caroline Honaiser Lescano, Fabiola Zakia Monica, Cristina Pontes Vicente, Erich Vinicius De Paula, Sara Teresinha Olalla Saad, Mariana Lazarini
Summary: The RhoGAP protein ARHGAP21 plays a critical role in megakaryocytic differentiation and platelet function by regulating cytoskeletal rearrangement. Silencing of ARHGAP21 affects microtubule stability and cell spreading, while haploinsufficiency of Arhgap21 leads to enhanced platelet activation and thrombus formation. These findings suggest that ARHGAP21 may be a key protein in the control of platelet production and function.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Developmental Biology
Yuko Yoshida, Atsushi Yamada, Yoshihiro Akimoto, Kyoko Abe, Sachie Matsubara, Junri Hayakawa, Junichi Tanaka, Mitsuhiro Kinoshita, Tadashi Kato, Hiroaki Ogata, Akiko Sakashita, Kenji Mishima, Yoshiaki Kubota, Hayato Kawakami, Ryutaro Kamijo, Takehiko Iijima
Summary: Cdc42 plays a critical role in blood vessel formation in vascular endothelial cells, as shown by the abnormal vascular phenotypes in Cdc42 cKO mice and reduced vascular sprouting induced by VEGF-A. Electron microscopy revealed detachment of endothelial cells and macrophage-induced phagocytosis of dead endothelial cells in Cdc42 cKO mice.
DEVELOPMENTAL BIOLOGY
(2021)
Review
Physiology
Alexander Wirth, Evgeni Ponimaskin
Summary: The protein Cdc42 plays a crucial role in regulating physiological functions in various tissues and cells through multiple signaling pathways. This review focuses on the mechanisms and regulation of Cdc42 lipid modifications, as well as the functional importance of different lipids in regulating Cdc42 functions. The possible implications of Cdc42 lipidation in pathological conditions and diseases are also discussed.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Medicine, General & Internal
Gue-Tae Moon, Ji-Hyun Lee, Sang-Hyun Jeong, Song-Wan Jin, Young-Min Park
Summary: This study demonstrated that NX-5 can suppress melanoma metastasis by reducing the expression levels of Rho-family GTPases, including decreasing migration and regulating the expression of related proteins.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Hematology
Diamantis G. Konstantinidis, Katie M. Giger, Mary Risinger, Suvarnamala Pushkaran, Ping Zhou, Phillip Dexheimer, Satwica Yerneni, Paul Andreassen, Ursula Klingmueller, James Palis, Yi Zheng, Theodosia A. Kalfa
Article
Developmental Biology
David M. Barry, Ke Xu, Stryder M. Meadows, Yi Zheng, Pieter R. Norden, George E. Davis, Ondine Cleaver
Review
Pharmacology & Pharmacy
Yuan Lin, Yi Zheng
EXPERT OPINION ON DRUG DISCOVERY
(2015)
Article
Biochemistry & Molecular Biology
Chris R. Evelyn, Jacek Biesiada, Xin Duan, Hong Tang, Xun Shang, Ruben Papoian, William L. Seibel, Sandra Nelson, Jaroslaw Meller, Yi Zheng
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Neurosciences
Juan Li, Lei Zhang, Zhenzhong Chen, Minjuan Xie, Lu Huang, Jinhua Xue, Yutong Liu, Nuyun Liu, Fukun Guo, Yi Zheng, Jiming Kong, Lin Zhang, Lu Zhang
NEUROBIOLOGY OF DISEASE
(2015)
Article
Multidisciplinary Sciences
Inuk Zandvakili, Ashley Kuenzi Davis, Guodong Hu, Yi Zheng
Article
Multidisciplinary Sciences
Kyung Hee Chang, Ramesh C. Nayak, Swarnava Roy, Ajay Perumbeti, Ashley M. Wellendorf, Katie Y. Bezold, Megan Pirman, Sarah E. Hill, Joseph Starnes, Anastacia Loberg, Xuan Zhou, Tadashi Inagami, Yi Zheng, Punam Malik, Jose A. Cancelas
NATURE COMMUNICATIONS
(2015)
Article
Multidisciplinary Sciences
Constantinos M. Mikelis, May Simaan, Koji Ando, Shigetomo Fukuhara, Atsuko Sakurai, Panomwat Amornphimoltham, Andrius Masedunskas, Roberto Weigert, Triantafyllos Chavakis, Ralf H. Adams, Stefan Offermanns, Naoki Mochizuki, Yi Zheng, J. Silvio Gutkind
NATURE COMMUNICATIONS
(2015)
Article
Allergy
Jun-Qi Yang, Khalid W. Kalim, Yuan Li, Shuangmin Zhang, Ashwini Hinge, Marie-Dominique Filippi, Yi Zheng, Fukun Guo
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2016)
Article
Oncology
Yajie Zhu, Jitao Zhou, Hongwei Xia, Xiangzheng Chen, Meng Qiu, Juan Huang, Surui Liu, Qiulin Tang, Nan Lang, Zhen Liu, Ming Liu, Yi Zheng, Feng Bi
INTERNATIONAL JOURNAL OF ONCOLOGY
(2014)
Article
Immunology
Shuangmin Zhang, Diamantis G. Konstantinidis, Jun-Qi Yang, Benjamin Mizukawa, Khalid Kalim, Richard A. Lang, Theodosia A. Kalfa, Yi Zheng, Fukun Guo
JOURNAL OF IMMUNOLOGY
(2014)
Letter
Oncology
F. Guo, J. Li, S. Zhang, W. Du, S. Amarachintha, J. Sipple, J. Phelan, H. L. Grimes, Y. Zheng, Q. Pang
Article
Biochemistry & Molecular Biology
Salvatore Fabbiano, Mauricio Menacho-Marquez, Maria A. Sevilla, Julian Albarran-Juarez, Yi Zheng, Stefan Offermanns, Maria J. Montero, Xose R. Bustelo
MOLECULAR AND CELLULAR BIOLOGY
(2014)
Review
Biochemistry & Molecular Biology
Zheng Zhang, Ming Liu, Yi Zheng
Summary: The future of regenerative medicine hinges on understanding the role of stem cells in tissue generation and restoration. Rho GTPases play a crucial role in regulating various cellular processes related to cell dynamics, and their implications in cancer stem cells open up new possibilities for cancer therapy.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2021)
Article
Oncology
Kee-Beom Kim, Dong-Wook Kim, Youngchul Kim, Jun Tang, Nicole Kirk, Yongyu Gan, Bongjun Kim, Bingliang Fang, Jae-ll Park, Yi Zheng, Kwon-Sik Park
Summary: This study characterizes the activity of the WNT pathway in small cell lung cancer (SCLC) and identifies the key role of the WNT5A-RHOA axis in SCLC progression. It provides potential targets for therapeutic interventions and biomarkers to improve patient treatment.