Article
Medicine, Research & Experimental
Catherine T. Le, Logan V. Vick, Craig Collins, Cordelia Dunai, Michael K. Sheng, Lam T. Khuat, Isabel Barao, Sean J. Judge, Ethan G. Aguilar, Brendan Curti, Maneesh Dave, Dan L. Longo, Bruce R. Blazar, Robert J. Canter, Arta M. Monjazeb, William J. Murphy
Summary: Memory T cells can be activated through bystander activation with cytokine signaling, but this response is inhibited by the PD-1 pathway. PD-1 blockade reverses this inhibition, but leads to activation-induced cell death and eventual loss of T cells.
Article
Medicine, Research & Experimental
Mengyan Li, Scott J. Garforth, Kaitlyn E. O'Connor, Hang Su, Danica M. Lee, Alev Celikgil, Rodolfo J. Chaparro, Ronald D. Seidel, R. Brad Jones, Ravit Arav-Boger, Steven C. Almo, Harris Goldstein
Summary: In this study, synTacs were used to expand highly functional HIV- and CMV-specific CD8(+) T cells, which showed potent antiviral activities in vivo. The results suggest that synTac-based approaches could be a promising therapeutic strategy for HIV cure and treatment of CMV and other viral infections.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Immunology
Hongyan Ren, Tianyu Zhang, Yongren Wang, Qi Yao, Ziyu Wang, Luyao Zhang, Lixin Wang
Summary: Research has shown that DRibbles can efficiently induce proliferation and activation of human B cells, leading to the production of chemokines, cytokines and hematopoietic growth factors. Human B cells can effectively phagocytose DRibbles and cross-present antigens to activate memory T cells. These findings suggest the potential for clinical application of B-DRibbles vaccines.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Vita Golubovskaya, Hua Zhou, Feng Li, Michael Valentine, Jinying Sun, Robert Berahovich, Shirley Xu, Milton Quintanilla, Man Cheong Ma, John Sienkiewicz, Yanwei Huang, Lijun Wu
Summary: CAR-T cell therapy, specifically targeting CD19, has been approved by the FDA for leukemia and lymphoma treatment. This study introduces novel CD37 and CD37-CD19 CAR-T cells, demonstrating their specific targeting abilities and laying the foundation for future clinical studies.
Article
Multidisciplinary Sciences
Senichiro Yanagawa, Hiroyuki Tahara, Takayuki Shirouzu, Shintaro Kawai, Yuka Tanaka, Kentaro Ide, Shuji Akimoto, Hideki Ohdan
Summary: This study aimed to establish a humanized mouse model for analyzing the mechanism of anti-allogeneic human B cell tolerance induction. By injecting NSG mice with PBMCs mixed with different HLA antigenic combinations, sufficient human total-IgG and anti-HLA antibodies were produced, but allospecific anti-HLA antibody production was suppressed.
Article
Microbiology
Jerome Kervevan, Aurelie Bouteau, Juliane S. Lanza, Adele Hammoudi, Sandra Zurawski, Mathieu Surenaud, Lydie Dieudonne, Marion Bonnet, Cecile Lefebvre, Hakim Hocini, Romain Marlin, Aurelie Guguin, Barbara Hersant, Oana Hermeziu, Elisabeth Menu, Christine Lacabaratz, Jean-Daniel Lelievre, Gerard Zurawski, Veronique Godot, Sandrine Henri, Botond Z. Igyarto, Yves Levy, Sylvain Cardinaud
Summary: Targeting of skin Langerhans cells (LC) may efficiently induce potent anti-HIV immune responses, serving as a promising strategy for developing effective HIV-1 vaccines. This approach demonstrates the potential of directing immune responses through specific cell targeting and may lead to the induction of protective antibodies against HIV-1.
Article
Allergy
Daniel Lozano-Ojalvo, Scott R. Tyler, Carlos J. Aranda, Julie Wang, Scott Sicherer, Hugh A. Sampson, Robert A. Wood, A. Wesley Burks, Stacie M. Jones, Donald Y. M. Leung, Maria Curotto de Lafaille, M. Cecilia Berin
Summary: This study observed that oral immunotherapy can increase the frequency of Tregs in individuals with lasting clinical tolerance. It also found that the release of IL-2 from Th2 effector cells can induce delayed and persistent activation of Tregs, which could be significant for improving current oral immunotherapy approaches.
Article
Gastroenterology & Hepatology
Ajinkya Pattekar, Lena S. Mayer, Chi Wai Lau, Chengyang Liu, Olesya Palko, Meenakshi Bewtra, Hpap Consortium, Lisa C. Lindesmith, Paul D. Brewer-Jensen, Ralph S. Baric, Michael R. Betts, Ali Naji, E. John Wherry, Vesselin T. Tomov
Summary: The study identified immune correlates of NoV protection and persistence and defined the breadth, distribution, and properties of human NoV-specific T-cell immunity, especially in the intestinal mucosa. These findings can guide the development of future vaccines and novel cellular therapeutics.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Angela Wahl, Wenbo Yao, Baolin Liao, Morgan Chateau, Cara Richardson, Lijun Ling, Adrienne Franks, Krithika Senthil, Genevieve Doyon, Fengling Li, Josh Frost, Christopher B. Whitehurst, Joseph S. Pagano, Craig A. Fletcher, M. Andrea Azcarate-Peril, Michael G. Hudgens, Allison R. Rogala, Joseph D. Tucker, Ian McGowan, R. Balfour Sartor, J. Victor Garcia
Summary: Germ-free mice are commonly used in studying the role of the microbiome in health and disease, but they are not suitable for studying human-specific pathogens. In this study, a germ-free mouse model systemically reconstituted with human immune cells was developed and used to evaluate the role of the resident microbiome in the acquisition, replication, and pathogenesis of Epstein-Barr virus (EBV) and human immunodeficiency virus (HIV). The results showed that the resident microbiome enhances the establishment of EBV infection, EBV-induced tumorigenesis, mucosal HIV acquisition, and replication.
NATURE BIOTECHNOLOGY
(2023)
Article
Cell & Tissue Engineering
Ksenia Bezverbnaya, Joanne A. Hammill, Derek Cummings, Bojana Bojovic, Bella Groisman, Christopher L. Baker, Craig Aarts, Danielle L. Hayes, Donna Rill, Stacey X. Xu, Andreas G. Bader, Christopher W. Helsen, Jonathan L. Bramson
Summary: B-cell maturation antigen (BCMA) is a clinically validated target for multiple myeloma. The authors have developed a fully humanized BCMA-specific T-cell antigen coupler (TAC) receptor. Optimization of the BCMA-specific TAC receptor was achieved through iterations of the BCMA-TAC design.
Article
Biochemistry & Molecular Biology
Marta Gomez-Perosanz, Jose L. Sanchez-Trincado, Miguel Fernandez-Arquero, John Sidney, Alessandro Sette, Esther M. Lafuente, Pedro A. Reche
Summary: This study identified and characterized 23 conserved HRV-specific CD8 T cell epitopes on PBMCs from 14 HLA I typed subjects, confirming peptide-specific IFN gamma production and binding to the relevant HLA I for nine of these epitopes. The study also validated cytotoxicity mediated by A*02:01-restricted epitopes and discovered an unusually long 16-mer epitope peptide restricted by A*02:01. These HRV-specific CD8 T cell epitopes are expected to elicit CD8 T cell responses in up to 87% of the population and could be crucial for developing an HRV vaccine.
Article
Oncology
Marta Compte, Seandean L. Harwood, Ainhoa Erce-Llamazares, Antonio Tapia-Galisteo, Eduardo Romero, Irene Ferrer, Eva M. Garrido-Martin, Ana B. Enguita, Maria C. Ochoa, Belen Blanco, Marta Oteo, Nekane Merino, Daniel Nehme-Alvarez, Oana Hangiu, Carmen Dominguez-Alonso, Manuela Zonca, Angel Ramirez-Fernandez, Francisco J. Blanco, Miguel A. Morcillo, Ines G. Munoz, Ignacio Melero, Jose L. Rodriguez-Peralto, Luis Paz-Ares, Laura Sanz, Luis Alvarez-Vallina
Summary: The researchers developed a humanized EGFR-specific 4-1BB-agonistic trimerbody, which showed significant antitumor activity in a wide range of human tumors without hepatotoxicity. Combining the trimerbody with a PD-L1 blocker led to increased IFN gamma secretion and resulted in tumor regression in aggressive triple-negative breast cancer humanized mice.
CLINICAL CANCER RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Esen Sefik, Benjamin Israelow, Haris Mirza, Jun Zhao, Rihao Qu, Eleanna Kaffe, Eric Song, Stephanie Halene, Eric Meffre, Yuval Kluger, Michel Nussenzweig, Craig B. Wilen, Akiko Iwasaki, Richard A. Flavell
Summary: This study introduces a humanized mouse model of COVID-19 that replicates the immune response and pathological features of the infection, providing a valuable tool for investigating disease mechanisms and treatment options.
NATURE BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Su Yin Lim, Sara Alavi, Zizhen Ming, Elena Shklovskaya, Carina Fung, Ashleigh Stewart, Helen Rizos
Summary: Our study found that tumor necrosis factor alpha (TNF alpha) can influence the differentiation status and immune inhibition of melanoma cells, with different impacts on different melanoma cells. TNF alpha showed poor induction of antigen presentation molecules but readily induced the PD-L2 immune checkpoint in melanoma cells. Our results suggest that TNF alpha promotes dynamic changes in melanoma cells that may favor immunotherapy resistance.
Article
Oncology
Corinna Grunert, Gerald Willimsky, Caroline Anna Peuker, Simone Rhein, Leo Hansmann, Thomas Blankenstein, Eric Blanc, Dieter Beule, Ulrich Keller, Antonio Pezzutto, Antonia Busse
Summary: T cell-based immunotherapy has shown remarkable clinical responses in cancer patients. However, isolating neoepitope-specific T cell receptors (TCRs) from the patients' own repertoire has limited success. This study suggests that using T cell repertoires from healthy donors and transgenic mice may be a viable strategy for isolating TCRs with known specificity against neoantigens.
Article
Cell Biology
Jilong Ren, Dawei Yu, Rui Fu, Peipei An, Renren Sun, Zhengzhu Wang, Runfa Guo, Haoyun Li, Ying Zhang, Ziyi Li, Yong-Guang Yang, Wei Li, Tang Hai, Zheng Hu
CELL PROLIFERATION
(2020)
Article
Immunology
Mohsen Khosravi-Maharlooei, HaoWei Li, Markus Hoelzl, Guiling Zhao, Amanda Ruiz, Aditya Misra, Yang Li, Nato Teteloshvili, Grace Nauman, Nichole Danzl, Xiaolan Ding, Elisha Y. Pinker, Aleksandar Obradovic, Yong-Guang Yang, Alina Iuga, Remi J. Creusot, Robert Winchester, Megan Sykes
Summary: This study evaluated the role of the thymus in development of multi-organ autoimmunity in HIS mice and found that T cells were crucial for disease development. Mice with a native mouse thymus developed disease earlier than those with a thymocyte-depleted human thymus graft. Failure to tolerize human T cells recognizing mouse antigens may explain eventual disease development.
JOURNAL OF AUTOIMMUNITY
(2021)
Review
Cell Biology
Long Su, Zheng Hu, Yong-Guang Yang
Summary: CXCR4 is expressed on leukemia cells and hematopoietic stem cells, and its regulation plays a crucial role in the retention and migration of these cells in the bone marrow microenvironment. While CXCR4 antagonists can mobilize leukemia cells for effective eradication, drug-resistant leukemia cells may not be affected by these antagonists, indicating alternative pathways in leukemia cell migration.
CELL PROLIFERATION
(2021)
Article
Biochemistry & Molecular Biology
Ye Wang, Wei Shen, Shuhan Liu, Ge Zhu, Xiandi Meng, Kuirong Mao, Jialiang Wang, Yong-Guang Yang, Chunsheng Xiao, Tianmeng Sun
Summary: The synthetic cationic anticancer polypeptide (ACPP) can induce immunogenic cancer cell death (ICD), enhance dendritic cell activation, increase natural killer cell levels, and effectively reprogram the tumor immunosuppressive microenvironment, with the anticancer immunity induced by ACPP being dose-dependent and capable of completely eliminating established tumors in 40% of cases.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Immunology
Long Su, Ming-Hui Fang, Jun Zou, Su-Jun Gao, Xiao-Yi Gu, Xian-Di Meng, Xue Wang, Zheng Hu, Yong-Guang Yang
Summary: The study showed that posttransplant treatment with the CXCR4 antagonist AMD3100 significantly improved the eradication of leukemia cells in the BM, enhanced GVL effects, and promoted donor hematopoietic engraftment. Additionally, posttransplant treatment with AMD3100 had no detectable deleterious effect related to GVHD.
CELLULAR & MOLECULAR IMMUNOLOGY
(2021)
Article
Immunology
Yixin Wang, Lei Wang, Cong Fu, Xue Wang, Siyao Zuo, Chang Shu, Yanhong Shan, Jin He, Qi Zhou, Wei Li, Yong-Guang Yang, Zheng Hu, Shucheng Hua
Summary: This study utilized a humanized mouse model with human lung tissue xenografts to investigate the immunopathogenesis and therapeutic interventions of viral respiratory diseases. The findings highlight the importance of tissue-resident memory T cells and viral Ag-specific T cells in the immune response against respiratory viruses.
JOURNAL OF IMMUNOLOGY
(2022)
Review
Materials Science, Biomaterials
Ge Zhu, Yong-Guang Yang, Tianmeng Sun
Summary: Vaccination plays a crucial role in preventing diseases, but there are challenges in clinical trials. The development of drug delivery systems may help improve the efficacy of vaccines.
BIOMATERIALS SCIENCE
(2022)
Article
Surgery
Yue Hou, Yanbao Xin, Shuhan Liu, Yong Li, Xiandi Meng, Jialiang Wang, Zhonggao Xu, Tianmeng Sun, Yong-Guang Yang
Summary: In this study, a nanotechnology-based therapy targeting TSP-1 was developed to prevent renal ischemia-reperfusion injury (IRI). The biocompatible nanoparticles effectively adsorbed and neutralized TSP-1, providing strong protection against renal IRI.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Review
Cell & Tissue Engineering
Ying Li, Jie Hao, Zheng Hu, Yong-Guang Yang, Qi Zhou, Liguang Sun, Jun Wu
Summary: This study summarized 79 clinical trials involving the use of MSCs to prevent or treat GVHD, analyzing their characteristics and results. The researchers aimed to gain a comprehensive understanding of the progress, challenges, limitations, and future trends in the field, and provide new perspectives for the design of future trials.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Jilong Ren, Dawei Yu, Jing Wang, Kai Xu, Yanan Xu, Renren Sun, Peipei An, Chongyang Li, Guihai Feng, Ying Zhang, Xiangpeng Dai, Hongye Zhao, Zhengzhu Wang, Zhiqiang Han, Haibo Zhu, Yuchun Ding, Xiaoyan You, Xueqin Liu, Meng Wu, Lin Luo, Ziyi Li, Yong-Guang Yang, Zheng Hu, Hong-Jiang Wei, Liangpeng Ge, Tang Hai, Wei Li
Summary: We successfully generated pig models with severe immunodeficiency that could construct human liver tissues.
CELL AND BIOSCIENCE
(2022)
Article
Medicine, Research & Experimental
Minghui Fang, Jun Zou, Fei Xu, Xue Wang, Shucheng Hua, Qi Zhou, Yong-guang Yang, Zheng Hu
Summary: The development of an animal model to study immune responses to pig vessels is crucial for improving immunosuppressive strategies in clinical pig organ transplantation.
XENOTRANSPLANTATION
(2023)
Article
Cell Biology
Xiaoling Zhang, Xianling Cong, Xiangting Jin, Yu'e Liu, Tong Zhang, Xinyuan Fan, Xiyao Shi, Xiaoying Zhang, Xue Wang, Yong-Guang Yang, Xiangpeng Dai
Summary: The transcription factor MYCN is frequently amplified and overexpressed in various cancers, and is considered undruggable. In this study, BRCA1-associated protein-1 (BAP1) is identified as an upstream regulator that stabilizes MYCN through direct binding. Depletion of BAP1 inhibits neuroblastoma tumor cell growth and migration, and confers cellular resistance to BET protein inhibitor JQ1 and Aurora A kinase inhibitor Alisertib. Our findings suggest that BAP1 could be a potential therapeutic target for MYCN-amplified neuroblastoma.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Rui Han, Wenjie Zhao, Xu Gu, Xue Gao, Yong-Guang Yang, Xiaoling Zhang
Summary: In this study, orthotopic and subcutaneous CDX neuroblastoma models were compared. The orthotopic model showed faster tumor growth, increased expression of PHOX2B and GAB2, and exhibited multiple organ metastasis resembling patients, while subcutaneous model mainly had lung metastasis. Both models responded similarly to cyclophosphamide treatment.
Article
Immunology
Dan Li, Wei Xiong, Yuande Wang, Jin Feng, Yuexi He, Juan Du, Jing Wang, Meixiang Yang, Hui Zeng, Yong-Guang Yang, Ning Wu, Shasha Chen, Zhongjun Dong
Summary: The interaction of SIRP alpha with CD47 is an important mechanism for preventing macrophage phagocytosis, but there are poorly defined CD47-independent mechanisms. In this study, the researchers discovered that SLAM family receptors (SFRs) play a critical role in constraining macrophage phagocytosis. These receptors are able to inhibit eat me signals and work in combination with CD47. This tolerance mechanism is compromised in patients with blood diseases, and the loss of SFRs enhances macrophage rejection of tumors.
SCIENCE IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Ting Li, Yanan Lv, Renren Sun, Yong-Guang Yang, Zheng Hu, Guoyue Lv
Summary: The lack of recipient CD47-donor SIRP alpha interaction does not significantly exacerbate platelet and RBC depletion following liver xenotransplantation. Studies using syngeneic mouse liver transplantation model and rat liver xenotransplantation model suggest that CD47-SIRP alpha signaling is not a significant risk factor for LXT-induced thrombocytopenia and anemia.
XENOTRANSPLANTATION
(2021)