Article
Geriatrics & Gerontology
Yuhei Mizunoe, Masaki Kobayashi, Hiroki Saito, Akifumi Goto, Ryota Migitaka, Kumi Miura, Naoyuki Okita, Yuka Sudo, Ryoma Tagawa, Miki Yoshida, Ai Umemori, Yoshimi Nakagawa, Hitoshi Shimano, Yoshikazu Higami
Summary: Research indicates that aging leads to muscle atrophy, but long-term caloric restriction can reduce this age-related muscle decline.
EXPERIMENTAL GERONTOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Luke K. Fuerniss, Bradley J. Johnson
Summary: This study validated a semi-automated workflow that can efficiently and objectively identify the type and measure the size of muscle fibers compared to a manual technique. The semi-automated workflow is six times faster than the manual workflow and yields similar results. This study demonstrates the efficiency and objectivity of the semi-automated workflow in imaging, classifying, and measuring muscle fibers.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Food Science & Technology
Tertius A. Kohn, Megan J. Anley, S'thandiwe N. Magwaza, Luqmaan Adamson, Louw C. Hoffman, Tertius S. Brand
Summary: This study analyzed muscle fiber type, fiber cross-sectional area, enzyme activities, and glycogen content in four muscles of 24 ostriches. The results showed similar proportions of type I and II fibers in all muscles, with the smallest fibers in the iliotibialis cranialis muscle. Citrate synthase activity was highest in the iliotibialis cranialis muscle and similar in the other muscles. 3-hydroxyacetyl Co A dehydrogenase activities were low in all muscles, indicating poor beta-oxidation. The iliotibialis cranialis muscle also had the lowest phosphofructokinase activity. Glycogen content varied within each muscle. These findings suggest that the ostrich muscles have low fat oxidation capacity and glycogen content, which could affect meat quality attributes.
Article
Biochemistry & Molecular Biology
Giuliana Abbadessa, Eleonora Maniscalco, Loredana Grasso, Jasmin Popara, Federica Di Scipio, Francesco Franco, Daniele Mancardi, Fabio Pigozzi, Paolo Borrione, Giovanni Nicolao Berta, Silvia Racca
Summary: Metformin (Met) is a commonly prescribed drug for type 2 diabetes mellitus. It suppresses hepatic gluconeogenesis, enhances peripheral glucose uptake, and lowers glucose absorption by the intestine. Recent studies have found that Met is also effective in other clinical applications. However, the mechanism of action of Met on muscle and its effect on muscle performance are still unclear. This study investigated the effects of Met combined with training on physical performance in healthy rats. The results showed that Met did not improve physical performance, but it protected myocytes from exercise-induced damage and positively affected muscle adaptation to exercise. This supports the idea of using Met therapeutically when muscle function and structure are compromised.
Article
Biochemistry & Molecular Biology
Javier Botella, Nicholas A. Jamnick, Cesare Granata, Amanda J. Genders, Enrico Perri, Tamim Jabar, Andrew Garnham, Michael Lazarou, David J. Bishop
Summary: Autophagy is an important mechanism for cells to degrade old or dysfunctional proteins and organelles. The effects of exercise on autophagosome content markers differ between rodents and humans. The decrease in LC3B-II protein levels induced by exercise in humans does not reflect a decreased autophagy flux.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Winfried Harzer, Antje Augstein, Diana Juenger, Christiane Keil, Bernhard Weiland
Summary: The study found that Notch 1-3 and CD34 were significantly upregulated in prognathic patients, but MyoD expression remained unchanged, indicating a high level of stimulation for satellite cells and maintenance of the regeneration cell pool. In contrast, retrognathic patients showed lower expression of Notch and satellite cells, which may be responsible for weak functional adaptation.
JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY
(2021)
Article
Biochemistry & Molecular Biology
Ephraim B. Winzer, Antje Schauer, Erik Langner, Antje Augstein, Keita Goto, Anita Mannel, Peggy Barthel, Anett Jannasch, Siegfried Labeit, Norman Mangner, Axel Linke, Volker Adams
Summary: This study aimed to investigate the impact of Empagliflozin on SKM function and mitochondrial function in an established HFpEF rat model. The results showed that Empagliflozin treatment improved SKM function and contractility, reduced intramuscular lipid content, and had positive effects on mitochondrial function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Pablo E. Morales, Matias Monsalves-Alvarez, Satya Murthy Tadinada, Matthew P. Harris, Andrea Ramirez-Sagredo, Jafet Ortiz-Quintero, Mayarling Francisca Troncoso, Nicole De Gregorio, Ximena Calle, Renata O. Pereira, Vitor A. Lira, Alejandra Espinosa, E. Dale Abel, Sergio Lavandero
Summary: The study found that high-fat diet induced differential regulation of autophagy and mitochondrial activity in different skeletal muscles of mice. After high-fat diet feeding, oxidative soleus muscle showed increased OXPHOS subunits and mitochondrial DNA content, while gastrocnemius muscle exhibited an increase in mitochondrial mass only after 40 weeks. Defects in autophagy may alter mitochondrial respiratory activity in response to obesogenic diet.
Article
Agriculture, Multidisciplinary
Peiyuan Li, Sha Zhang, Hui Song, Stanislav Seydou Traore, Jiangtao Li, David Raubenheimer, Zhenwei Cui, Guangning Kou
Summary: Naringin can promote the transition of skeletal muscle fibers from fast-twitch to slow-twitch through the AdipoR1 signaling pathway, improving exercise endurance and muscle fiber deficiencies related diseases.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Diego Salagre, Enrique Raya Alvarez, Cruz Miguel Cendan, Samira Aouichat, Ahmad Agil
Summary: This study aimed to evaluate the effects of melatonin on the molecular and morphological levels of the red vastus lateralis muscle in obese rat models. The results showed that melatonin partially restored mitochondrial imbalance, enhanced autophagy, improved oxidative phenotype, and reduced oxidative stress in skeletal muscle. These findings suggest that melatonin may have potential therapeutic implications for obesity-induced skeletal muscle metabolic inflexibility among patients with obesity and type 2 diabetes mellitus.
Article
Chemistry, Multidisciplinary
Yoonhee Jin, Dena Shahriari, Eun Je Jeon, Seongjun Park, Yi Sun Choi, Jonghyeok Back, Hyungsuk Lee, Polina Anikeeva, Seung-Woo Cho
Summary: The passage highlights the need for interventions to induce functional skeletal muscle restoration, with a focus on tissue-engineered muscle constructs. It mentions the current challenges in reliable cell sources and tissue scaffold engineering for tissue-engineering treatments.
ADVANCED MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Paolo Raffa, Maria Easler, Francesca Cecchinato, Beatrice Auletta, Valentina Scattolini, Silvia Perin, Mattia Francesco Maria Gerli, Paola Caccin, Nicola Elvassore, Paolo De Coppi, Anna Urciuolo
Summary: The study successfully developed a 3D model of neuromuscular tissue using decellularized skeletal muscle constructs, which demonstrated functional contraction and maintained viability of multiple cell types. Additionally, a customized culture system was developed to facilitate easy access, imaging, and analysis of the in vitro engineered co-cultures.
APPLIED SCIENCES-BASEL
(2021)
Article
Nutrition & Dietetics
Kenji Uchida, Mina Fujitani, Takafumi Mizushige, Fuminori Kawabata, Kohsuke Hayamizu, Keisuke Uozumi, Yuma Hara, Mariko Sawai, Ryota Uehigashi, Shinji Okada, Naoko Goto-Inoue, Mizuki Morisasa, Taro Kishida
Summary: This study found that dietary intake of Alaska pollack protein (APP) can induce acute and sustainable skeletal muscle hypertrophy in rats, regardless of dietary fat content. APP can be an approach for maintaining or increasing muscle mass.
Article
Biochemistry & Molecular Biology
Lingli Feng, Jinglei Si, Jingwei Yue, Mingwei Zhao, Wenjing Qi, Siran Zhu, Jiayuan Mo, Lixian Wang, Ganqiu Lan, Jing Liang
Summary: This study utilized RNA-seq and ATAC-seq to analyze muscle samples from Landrace pigs at different time points after birth. It identified ACBD7, TMEM220, and ATP1A2 as key genes related to porcine skeletal muscle development and also identified potential regulatory elements. Additionally, the results showed the importance of functional elements related to lipid metabolism and cell cycle in porcine skeletal muscle development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Yan Li, Qingshan Ma, Xiaoyuan Shi, Wenmin Yuan, Guiqin Liu, Changfa Wang
Summary: This study systematically compared the mRNA and microRNA transcriptomes of two different muscle tissues in donkeys and identified differentially expressed genes and miRNAs that may play a role in regulating muscle fiber types. The study provides valuable insights into the molecular mechanisms involved in muscle fiber composition and may contribute to improving meat quality traits in donkeys.
Article
Food Science & Technology
Issei Yokoyama, Yuji Nakai, Yoshihiro Suzuki, Motoko Ohata, Yusuke Komiya, Jun Nagasao, Keizo Arihara
JOURNAL OF FOOD SCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Takahiro Suzuki, Aika Mori, Takahiro Maeno, Rio Arimatsu, Emi Ichimura, Yuriko Nishi, Kouga Hisaeda, Yuki Yamaya, Ken Kobayashi, Mako Nakamura, Ryuichi Tatsumi, Koichi Ojima, Takanori Nishimura
Summary: Studies have shown that netrin-1 is expressed at higher levels in satellite cells derived from fast muscles compared to those from slow muscles. Knockdown of netrin-1 can decrease the expression of fast-type myosin heavy chain, while exogenous netrin-1 can upregulate it in satellite cells, suggesting that netrin-1 synthesized in satellite cells from fast muscles may promote myofiber type commitment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Alaa Elgaabari, Atsuko Miyawaki-Kuwakado, Kosuke Tomimatsu, Qianmei Wu, Kosuke Tokunaga, Wakana Izumi, Takahiro Suzuki, Ryuichi Tatsumi, Mako Nakamura
Summary: The ectopic expression of Pax7 results in repression of adipocyte genes and induction of skeletal muscle cell genes by promoting the formation of H3K27me3 at adipocyte-related gene loci. It suggests that the primary function of ectopic Pax7 expression is the formation of H3K27me3, leading to the regulation of muscle gene expression as a secondary effect.
JOURNAL OF BIOCHEMISTRY
(2021)
Article
Nutrition & Dietetics
Yusuke Komiya, Makoto Sugiyama, Masaru Ochiai, Nanako Osawa, Yuto Adachi, Shugo Iseki, Keizo Arihara
Summary: The study found that feeding a diet containing olive oil can enhance the endurance of mice and increase the accumulation of intramuscular triacylglycerol. This increased endurance is independent of muscle fiber type. Additionally, no metabolic impairments or differences in growth profiles were observed in mice fed with the olive oil diet compared to the controls.
Article
Chemistry, Applied
Masaru Ochiai, Yusuke Komiya
Summary: Research found that crickets had higher levels of choline-binding PLs compared to migratory locusts and silkworms; crickets were rich in n-6 polyunsaturated fatty acids, while migratory locusts and silkworms were rich in n-3 polyunsaturated fatty acids.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Food Science & Technology
Masaya Ogata, Jumpei Uchiyama, Abdulatef M. Ahhmed, Seiichi Sakuraoka, Satoshi Taharaguchi, Ryoichi Sakata, Wataru Mizunoya, Shiro Takeda
Summary: The study investigated the inherent bacteria contributing to ACE inhibitory and antioxidant activities in dry-cured meat products. Results showed that different species of Lactobacillales order were important for the functional activities of the products.
Article
Nutrition & Dietetics
Yusuke Komiya, Makoto Sugiyama, Chika Koyama, Satoshi Kameshima, Masaru Ochiai, Yuto Adachi, Issei Yokoyama, Hanae Fukasawa, Kazuki Yoshioka, Keizo Arihara
Summary: Research has found that consuming olive oil can cause hepatic lipid accumulation in female mice, but it does not result in liver damage or inflammation. Compared to the control group, the expression of genes related to triacylglycerol and fatty acid synthesis was significantly upregulated in the liver of female mice fed with olive oil. These findings contribute to a better understanding of the impact of dietary oils on sex-dependent hepatic lipid metabolism.
NUTRITION RESEARCH
(2023)
Article
Food Science & Technology
Issei Yokoyama, Ou Setoyama, Yaqi Jia, Nana Fujita, Akane Waki, Yusuke Komiya, Jun Nagasao, Keizo Arihara
Summary: Food-derived dipeptides LK and KL exhibit antioxidant and antiglycation activity, prolong the lifespan of C. elegans, and suppress intracellular ROS and blue autofluorescence levels.
FOOD SCIENCE & NUTRITION
(2023)
Article
Biology
Shoko Sawano, Misaki Fukushima, Taiki Akasaka, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi, Wataru Mizunoya
Summary: Skeletal muscle atrophy due to inactivity leads to changes in gene expression. However, little is known about the patterns of up-and downregulated gene expression during long-term and equilibrated muscle atrophy. This study used RNA-Seq to examine the changes in gene expression in long-term denervated mouse muscles. The results revealed that the expression of several genes, including E230016M11Rik, was significantly upregulated in both soleus and extensor digitorum longus (EDL) muscles.
Article
Oncology
Takahiro Maeno, Rio Arimatsu, Koichi Ojima, Yuki Yamaya, Hikaru Imakyure, Naruha Watanabe, Yusuke Komiya, Ken Kobayashi, Mako Nakamura, Takanori Nishimura, Ryuichi Tatsumi, Takahiro Suzuki
Summary: Satellite cells play a crucial role in skeletal muscle regeneration and hypertrophy through the formation of nascent myofibers. This study investigated the role of netrin-4, a multi-potent modulator, in regulating myofiber type commitment and myotube formation. The findings suggest that satellite cells synthesize netrin-4 during the initiation stage of myogenic differentiation to promote their own fusion through the MyoD-Myomixer signaling axis.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
Shiro Takeda, Abdulatef M. Ahhmed, Kazuki Sogawa, Saki Mouri, Sakurako Kaneko, Ryoichi Sakata, Wataru Mizunoya
Summary: Venison, a type of game meat, contains bioactive peptides with antioxidative properties. The study investigated the antioxidant activity of venison treated by in vitro cooking and digestion. The results showed that the digested venison had increased antioxidant activity and contained two peptides with high DPPH-RS activities.
ANIMAL SCIENCE JOURNAL
(2023)
Article
Agriculture, Dairy & Animal Science
Issei Yokoyama, Motoko Ohata, Yusuke Komiya, Jun Nagasao, Keizo Arihara
Summary: 2,5-Dimethyl-4-hydroxy-3(2H)-furanone (DMHF), a compound generated by the Maillard reaction, was detected in cooked meat samples from various animal species and parts. The amount of DMHF showed a significant positive correlation with the glucose content in the round and thigh parts. These findings suggest that DMHF generation during meat cooking may be regulated by the glucose content.
ANIMAL SCIENCE JOURNAL
(2023)
Article
Cell Biology
Alaa Elgaabari, Nana Imatomi, Hirochika Kido, Takashi Nakashima, Shoko Okuda, Yoshitaka Manabe, Shoko Sawano, Wataru Mizunoya, Ryuki Kaneko, Sakiho Tanaka, Takahiro Maeno, Yuji Matsuyoshi, Miyumi Seki, So Kuwakado, Kahona Zushi, Nasibeh Daneshvar, Mako Nakamura, Takahiro Suzuki, Kenji Sunagawa, Judy E. Anderson, Ronald E. Allen, Ryuichi Tatsumi
Summary: Mechanical perturbation triggers activation of myogenic stem cells, but with aging, extracellular HGF undergoes nitration and loses its binding with c-met, disturbing muscle homeostasis. These findings highlight the inhibitory effects of HGF nitration on myogenic stem cell dynamics and contribute to understanding age-related muscle atrophy and impaired regeneration.
Article
Biochemistry & Molecular Biology
Alaa Elgaabari, Nana Imatomi, Hirochika Kido, Miyumi Seki, Sakiho Tanaka, Yuji Matsuyoshi, Takashi Nakashima, Shoko Sawano, Wataru Mizunoya, Takahiro Suzuki, Mako Nakamura, Judy E. Anderson, Ryuichi Tatsumi
Summary: Protein tyrosine residue nitration is associated with changes in protein activity and function, and can be used to monitor the progression of pathological disorders. This study found that nitration affects muscle growth and regeneration, specifically impacting the functionality of hepatocyte growth factor. Nitration may perturb the dynamics of myogenic stem cells, leading to the progression of muscular disorders.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Alaa Elgaabari, Nana Imatomi, Hirochika Kido, Miyumi Seki, Sakiho Tanaka, Yuji Matsuyoshi, Takashi Nakashima, Shoko Sawano, Wataru Mizunoya, Takahiro Suzuki, Mako Nakamura, Judy E. Anderson, Ryuichi Tatsumi
Summary: This study investigated the impact of protein tyrosine residue nitration on muscle growth, regeneration, and pathological disorders. The findings suggest that nitration disrupts the function of hepatocyte growth factor and may stimulate the progression of muscular disorders and diseases.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)
