Article
Cell Biology
Meichao Zhang, Yiling Meng, Yingxia Ying, Pingting Zhou, Suning Zhang, Yong Fang, Yuan Yao, Dong Li
Summary: This study investigates the molecular programs involved in directed differentiation of myeloid progenitor cells. It identifies the role of STAT5 and STAT3 in the fate specification of these cells, with STAT3 promoting neutrophil differentiation and STAT5 promoting monocyte/macrophage differentiation. These findings provide new mechanistic insights into myeloid differentiation and may have implications for the diagnosis and treatment of diseases related to abnormal myeloid differentiation.
CELL DEATH DISCOVERY
(2023)
Article
Biochemistry & Molecular Biology
Javiera Villar, Lea Ouaknin, Adeline Cros, Elodie Segura
Summary: During inflammation, monocytes can differentiate into either macrophages or dendritic cells. This study used single-cell RNA sequencing and computational analysis to identify different pathways of differentiation for these two cell types. It also identified key transcription factors involved in monocyte fate commitment.
Article
Cell Biology
M. T. Longhi, L. E. Silva, M. Pereira, M. Magalhaes, J. Reina, F. N. L. Vitorino, B. M. Gumbiner, J. P. C. da Cunha, N. Cella
Summary: The study identified that the PI3K-Akt and JAK2-STAT3 pathways are involved in regulating EGF-induced maspin nuclear accumulation in MCF-10A cells, while the MAP kinase pathway is not. Cell-cell contact also plays a role in determining Maspin subcellular localization. Proteomic and interactome analyses suggest that Maspin may have effects on post-transcriptional and translation regulation, protein folding, and cell-cell adhesion.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Pharmacology & Pharmacy
Chao Hou, Dong Wang, Mingxia Zhao, Petek Ballar, Xinru Zhang, Qiong Mei, Wei Wang, Xiang Li, Qiang Sheng, Jun Liu, Chuansheng Wei, Yujun Shen, Yi Yang, Peng Wang, Juntang Shao, Sa Xu, Fuyan Wang, Yang Sun, Yuxian Shen
Summary: This study reveals that the neurotrophic factor, mesencephalic astrocyte-derived neurotrophic factor (MANF), is upregulated in fibrotic liver tissues and plays a role in hepatic fibrosis. MANF deficiency worsens hepatic fibrosis, while transfusion of MANF-sufficient macrophages ameliorates fibrosis. Mechanistically, MANF interacts with S100A8 to inhibit TLR4-NF-KB signaling activation. Additionally, recombinant human MANF administration reduces hepatic fibrosis. This study provides insight into the treatment of hepatic fibrosis.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Oncology
Xin Zhang, Bo Hu, Yun-Fan Sun, Xiao-Wu Huang, Jian-Wen Cheng, Ao Huang, Hai-Ying Zeng, Shuang-Jian Qiu, Ya Cao, Jia Fan, Jian Zhou, Xin-Rong Yang
Summary: Differentiation-inducing therapy using arsenic trioxide (ATO) was shown to effectively induce differentiation of hepatocellular carcinoma (HCC) cancer stem cells (CSCs) by downregulating CSC-related genes and suppressing tumorigenicity. Combinatorial treatment with ATO and 5-fluorouracil/cisplatin enhanced therapeutic effects through synergistic inhibition of the LIF/JAK1/STAT3 and NF-kB signaling pathways in HCC cells. These findings provide new insights for clinical treatment of HCC.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Article
Biology
Yuzhou Xue, Minghao Luo, Xiankang Hu, Xiang Li, Jian Shen, Wenyan Zhu, Longxiang Huang, Yu Hu, Yongzheng Guo, Lin Liu, Lingbang Wang, Suxin Luo
Summary: It has been discovered that during the progression of atherosclerosis, lipid deposits cause functional changes in macrophage-like vascular smooth muscle cells (VSMCs), which are primarily regulated by the IL-1 beta/STAT3 signaling pathway.
COMMUNICATIONS BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jiajia Wang, Jiaying Wang, Wenxiang Hong, Lulu Zhang, Liqian Song, Qi Shi, Yanfei Shao, Guifeng Hao, Chunyan Fang, Yueping Qiu, Lijun Yang, Zhaoxu Yang, Jincheng Wang, Ji Cao, Bo Yang, Qiaojun He, Qinjie Weng
Summary: Optineurin (OPTN) plays a crucial role in regulating dendritic cell (DC) maturation, where its deficiency leads to Jak2/Stat3 activation and IL-10 production, suppressing DC maturation and function to ameliorate autoimmune responses in mice.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Chen Zhao, Yang Zhao, Jiaqi Zhao, Guixian Meng, Shuyu Huang, Yichen Liu, Shanshan Wang, Ling Qi
Summary: MicroRNAs can be delivered from AML cells to HSCs via EVs to regulate hematopoietic function. MiR-548ac, abundant in AML-derived EVs, targets TRIM28 to inhibit hematopoiesis through STAT3 activation.
CANCER GENE THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Min Hee Yang, In Jin Ha, Jae-Young Um, Kwang Seok Ahn
Summary: Albendazole has been shown to inhibit the activation of oncogenic transcription factors STAT3 and STAT5 in gastric cancer cells through multiple mechanisms, including suppressing phosphorylation of JAK1/2 and Src, increasing SHP-1 protein levels, and inducing an imbalance of GSH/GSSG leading to elevated levels of reactive oxygen species.
Article
Multidisciplinary Sciences
Yale S. Michaels, John M. Edgar, Matthew C. Major, Elizabeth L. Castle, Carla Zimmerman, Ting Yin, Andrew Hagner, Charles Lau, Han Hsuan Hsu, M. Iliana Ibanez-Rios, Lauren J. Durland, David J. H. F. Knapp, Peter W. Zandstra
Summary: This study reported an efficient serum- and feeder-free system for differentiating human PSCs into hematopoietic progenitors and T cells. Providing DLL4 and VCAM1 during the endothelial-to-hematopoietic transition significantly enhanced T cell output. The research also established chemically defined medium formulations for T cells with diverse TCR repertoire.
Article
Biochemistry & Molecular Biology
Meng Zhao, Ariel Quintana, Chen Zhang, Alexander Y. Andreyev, William Kiosses, Tomomi Kuwana, Anne Murphy, Patrick G. Hogan, Mitchell Kronenberg
Summary: The differentiation of NKT2 cells requires higher and sustained calcium signals compared to their NKT1 counterparts, which is connected with mitochondria and cellular metabolism.
Article
Biochemistry & Molecular Biology
Sijin Cheng, Ziyi Li, Ranran Gao, Baocai Xing, Yunong Gao, Yu Yang, Shishang Qin, Lei Zhang, Hanqiang Ouyang, Peng Du, Liang Jiang, Bin Zhang, Yue Yang, Xiliang Wang, Xianwen Ren, Jin-Xin Bei, Xueda Hu, Zhaode Bu, Jiafu Ji, Zemin Zhang
Summary: This study conducted a pan-cancer analysis of single myeloid cells from 210 patients across 15 human cancer types, revealing distinct features of TIMs in different cancer types. For example, mast cells in nasopharyngeal cancer were associated with better prognosis, and pro-angiogenic TAMs showed diverse markers across different cancer types. The composition of TIMs appeared to be linked with certain features of somatic mutations and gene expressions.
Article
Multidisciplinary Sciences
Mark D. Bunting, Maulik Vyas, Marta Requesens, Adam Langenbucher, Erik B. Schiferle, Robert T. Manguso, Michael S. Lawrence, Shadmehr Demehri
Summary: NK cells undergo a switch in function from direct cytotoxicity to chemokine/cytokine production upon exiting the circulation. Blocking tumor collagen deposition enhances NK cell cytotoxicity against MHC-I-deficient melanoma.
Article
Immunology
Yongqiang Liao, Ni Xiao, Xiaoming Wang, Senhua Dai, Guiliang Wang
Summary: In this study, we found that the expression of Thymosin beta 4 x (Tmsb4x) is decreased in patients with septicemia, leading to increased inflammatory response and reduced proportion of dendritic cells (DCs). Restoration of Tmsb4x expression facilitated the differentiation of mononuclear cells into DCs.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Immunology
Rene Reitermaier, Thomas Krausgruber, Nikolaus Fortelny, Tanya Ayub, Pablo Augusto Vieyra-Garcia, Philip Kienzl, Peter Wolf, Anke Scharrer, Christian Fiala, Marita Kolz, Manuela Hiess, Martin Vierhapper, Christopher Schuster, Andreas Spittler, Christof Worda, Wolfgang Weninger, Christoph Bock, Rene Reitermaier, Adelheid Elbe-Buerger
Summary: Single-cell analyses identified a naive T cell population expressing specific T cell receptors enriched in fetal skin and intestine. These cells may contribute to early skin development and fetal immune defense, showing fundamental differences in immune surveillance between fetal and adult human skin.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)