Article
Chemistry, Medicinal
Peizhang Wu, Jun Guo, Hongwei Yang, Debin Yuan, Chaoxiang Wang, Zhong Wang
Summary: Exosomal miR-106a-5p derived from hypoxia glioma cells reduces the sensitivity of glioma cells to TMZ by downregulating PTEN, indicating a potential mechanism for chemoresistance which could provide new strategies for improving the clinical efficacy of TMZ in glioma treatment.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Ita Novita Sari, Ying-Gui Yang, Yoseph Toni Wijaya, Nayoung Jun, Sanghyun Lee, Kwang Seock Kim, Jeevisha Bajaj, Vivian G. Oehler, Soo-Hyun Kim, Soo-Young Choi, Sa-Hee Park, Dong-Wook Kim, Tannishtha Reya, Jaeseok Han, Hyog Young Kwon
Summary: AMD1 plays a crucial role in the progression of myeloid leukemia, and its depletion leads to differentiation of leukemic stem cells and impairs leukemia progression. Pharmacological inhibition of AMD1 can significantly reduce the progression of leukemia cells, offering a new therapeutic approach for the treatment of myeloid leukemia.
Editorial Material
Cell Biology
Mickie Bhatia, Amro Elrafie
Summary: In this study, Liu et al. show that Prmt7 can regulate the onset and progression of leukemia by inhibiting the self-renewal capacity of leukemic stem cells (LSCs) in a mouse model of chronic myeloid leukemia (CML).
Article
Chemistry, Medicinal
Jincai Wang, Xufeng Zhang, Fang Yang, Yuguang Yang, Tianjiao Wang, Wenming Liu, Hongfeng Zhou, Wei Zhao
Summary: The study demonstrated that RASSF1A and MAP1S were lowly expressed and positively correlated in NSCLC tissues, and RASSF1A enhances chemosensitivity in NSCLC by regulating MAP1S to activate autophagy via the Keap1-Nrf2 pathway.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)
Article
Cell Biology
Han Han, Hiroki J. Nakaoka, Line Hofmann, Jeff Jiajing Zhou, Clinton Yu, Lisha Zeng, Junyu Nan, Gayoung Seo, Rebecca Elizabeth Vargas, Bing Yang, Ruxi Qi, Lee Bardwell, Dmitry A. Fishman, Ken W. Y. Cho, Lan Huang, Ray Luo, Rahul Warrior, Wenqi Wang
Summary: The Hippo pathway was found to regulate heavy metal homeostasis by phosphorylating and inhibiting MTF1, thus attenuating the transcription of heavy metal response genes and protecting cells from heavy metal-induced toxicity. The activity of the Hippo pathway kinase LATS is also inhibited by heavy metal treatment, suggesting an interplay between heavy metals and the Hippo pathway.
NATURE CELL BIOLOGY
(2022)
Article
Oncology
Emese Toth, Ferenc Erdodi, Andrea Kiss
Summary: The major component of green tea, EGCG, activates Myosin Phosphatase (MP) and enhances cancer cell chemosensitivity. The combined treatment of EGCG and DNR significantly decreases leukemic cell viability by inducing apoptosis and reducing proliferation. These effects are achieved by EGCG-induced dephosphorylation of tumor suppressor proteins.
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Simona Soverini, Sara De Santis, Cecilia Monaldi, Samantha Bruno, Manuela Mancini
Summary: Chronic myeloid leukemia (CML) originates from the transformation of multipotent hematopoietic stem cells, with the resulting BCR-ABL1 fusion gene encoding a deregulated tyrosine kinase. Treatment with tyrosine kinase inhibitors (TKIs) can eliminate progenitor cells but not quiescent LSCs. Researchers have been working on identifying druggable targets for LSC eradication in CML.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Shu-Hui Su, Shu-Jem Su, Li-Yun Huang, Yun-Chen Chiang
Summary: Acidic lysosomes are crucial for cancer development and chemotherapy resistance. Chloroquine (CQ) and its analogues have been explored as potential solutions for overcoming cancer progression and chemoresistance. However, their efficacy in clinical trials has been modest. This study investigated the mechanisms of acquired resistance to CQ treatment in leukemic cells. The results showed that leukemic cells developed increased tolerance to low doses of CQ by producing more acidic organelles. Higher doses of CQ induced cytotoxicity and cell death, with an associated increase in mitochondrial activity. Co-treatment with an inhibitor of mitochondrial ATP synthase prevented the elevation of oxidants, pH, and stress on mitochondria, thus improving cell survival and proliferation. In addition, cells treated with the inhibitor displayed lysosomal exocytosis and microvesicle release, allowing them to exclude CQ and repair necrotic damage. The interconnection between lysosomes, mitochondria, and cytosol is crucial for leukemic susceptibility to lysosomotropic chemotherapeutics.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Multidisciplinary Sciences
Nguyen Thanh Huyen, Nguyen Thy Ngoc, Nguyen Hoang Giang, Do Thi Trang, Ha Hong Hanh, Vu Duc Binh, Nguyen Van Giang, Nguyen Xuan Canh, Nguyen Thi Xuan
Summary: This study explored the impact of CYLD on macrophage functions in healthy individuals and AML patients. The results showed that AML patients with low CYLD expression had higher proportions of M4/M5 subtypes. Low CYLD expression was also closely associated with older patients and elevated levels of LDH in AML. Additionally, knockdown of CYLD enhanced activation of STAT-1 in normal macrophages, leading to increased expression of maturation markers and IL-6 production, as well as suppression of cell apoptosis and phagocytosis. Furthermore, macrophage phagocytosis from AML M4/M5b was higher than that from healthy controls upon CYLD siRNA transfection through STAT1 signaling. In conclusion, the inhibitory effects of CYLD on macrophage functions are expected to impact the immune response in AML.
Article
Multidisciplinary Sciences
Kristina M. Garske, Asha Kar, Caroline Comenho, Brunilda Balliu, David Z. Z. Pan, Yash V. Bhagat, Gregory Rosenberg, Amogha Koka, Sankha Subhra Das, Zong Miao, Janet S. Sinsheimer, Jaakko Kaprio, Kirsi H. Pietilainen, Paivi Pajukanta
Summary: Obesity-induced adipose tissue dysfunction can lead to chronic low-grade inflammation and other comorbidities. This study used human primary preadipocytes from monozygotic twins with different body mass index (BMI) to investigate the impact of increased BMI on subnuclear chromatin compartmentalization and downstream inflammation. The results showed that open chromatin co-accessibility was altered in higher BMI twins compared to lower BMI twins, suggesting a mechanism through which obesity may induce inflammation through gene-environment interactions. Furthermore, variants within these regions were found to contribute to systemic inflammation in association with BMI and C-reactive protein levels.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Yang-Liu Song, Ming-Hui Yang, Si Zhang, Hao Wang, Kun-Lun Kai, Chun-Xia Yao, Fei-Fei Dai, Meng-Jiao Zhou, Jin-Biao Li, Zhi-Ru Wei, Zhongnan Yin, Wei-Guo Zhu, Lixiang Xue, Ming-Xi Zang
Summary: This study reveals that GATA-4 acts as a molecular switch for the regulation of miR-29a expression. It recruits polycomb group proteins like EZH2 to negatively regulate miR-29a in undifferentiated C2C12 myoblast cells. In poorly differentiated rhabdomyosarcoma cells, EZH2 still binds to the miR-29a promoter with GATA-4 to mediate transcriptional repression of miR-29a. However, during re-differentiation of rhabdomyosarcoma cells, EZH2 is displaced from the miR-29a promoter, leading to the activation of miR-29a and promoting myogenic differentiation.
Article
Multidisciplinary Sciences
Marian Raghubir, Syeda M. Azeem, Rifat Hasnat, Chowdhury N. Rahman, Linda Wong, Salina Yan, Yu Qi Huang, Raquel Zhagui, Angelina Blyufer, Iffat Tariq, Cassey Tam, Sonam Lhamo, Lucas Cecilio, Yesmin Chowdhury, Shraddha ChandThakuri, Shahana S. Mahajan
Summary: Our study demonstrates that Riluzole activates c-Abl kinase to regulate the pro-apoptotic activity of YAP in osteosarcoma. Knocking down YAP or c-Abl kinase inhibits the apoptosis induced by Riluzole.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Emmanuel Griessinger, Diego Pereira-Martins, Marielle Nebout, Claudie Bosc, Estelle Saland, Emiline Boet, Ambrine Sahal, Johanna Chiche, Delphine Debayle, Lucile Fleuriot, Maurien Pruis, Veronique De Mas, Francois Vergez, Christian Recher, Gerwin Huls, Jean-Emmanuel Sarry, Jan Jacob Schuringa, Jean-Francois Peyron
Summary: Dependency on mitochondrial oxidative phosphorylation (OxPhos) is a potential weakness for leukemic stem cells (LSC) that can be exploited for therapeutic purposes. Fatty acid oxidation (FAO) is a crucial OxPhos-fueling catabolic pathway for some acute myeloid leukemia (AML) cells, particularly chemotherapy-resistant AML cells. Cold sensitivity at 4℃ can selectively kill AML LSCs with active FAO supported OxPhos while sparing normal hematopoietic stem cells.
Article
Physiology
Devin B. Phillips, Nicolle J. Domnik, Amany F. Elbehairy, Megan E. Preston, Kathryn M. Milne, Matthew D. James, Sandra G. Vincent, Megha Ibrahim-Masthan, J. Alberto Neder, Denis E. O'Donnell
Summary: The study found that excessive exercise ventilation in mild COPD patients is not explained by altered central chemosensitivity.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2021)
Article
Immunology
Zhuan Wen, Yong Li, Bibo Tan, Zihao Chen, Qun Zhao, Ming Tan, Yijie Zhao, Yuxiang Xia, Liqiao Fan
Summary: This study revealed that LINC01088 expression is significantly reduced in gastric cancer tissues and cell lines. It inhibits the proliferation, invasion, and migration of gastric cancer cells through regulating the miR-95/LATS2 pathway via the ceRNA mechanism.
INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yuta Yamamoto, Takeru Makiyama, Takeshi Harita, Kenichi Sasaki, Yimin Wuriyanghai, Mamoru Hayano, Suguru Nishiuchi, Hirohiko Kohjitani, Sayako Hirose, Jiarong Chen, Fumika Yokoi, Taisuke Ishikawa, Seiko Ohno, Kazuhisa Chonabayashi, Hideki Motomura, Yoshinori Yoshida, Minoru Horie, Naomasa Makita, Takeshi Kimura
HUMAN MOLECULAR GENETICS
(2017)
Article
Cardiac & Cardiovascular Systems
Yimin Wuriyanghai, Takeru Makiyama, Kenichi Sasaki, Tsukasa Kamakura, Yuta Yamamoto, Mamoru Hayano, Takeshi Harita, Suguru Nishiuchi, Jiarong Chen, Hirohiko Kohjitani, Sayako Hirose, Fumika Yokoi, Jingshan Gao, Kazuhisa Chonabayashi, Ken Watanabe, Seiko Ohno, Yoshinori Yoshida, Takeshi Kimura, Minoru Horie
Article
Cell & Tissue Engineering
Tadashi Takaki, Azusa Inagaki, Kazuhisa Chonabayashi, Keiji Inoue, Kenji Miki, Seiko Ohno, Takeru Makiyama, Minoru Horie, Yoshinori Yoshida
STEM CELLS INTERNATIONAL
(2019)
Letter
Hematology
Kazuhisa Chonabayashi, Yoshinori Yoshida, Toshio Kitawaki, Yasuhito Nannya, Momoko Nakamura, Shinichiro Oshima, Masakatsu Hishizawa, Kouhei Yamashita, Seishi Ogawa, Akifumi Takaori-Kondo
ANNALS OF HEMATOLOGY
(2019)
Letter
Hematology
Fumiya Wada, Tadakazu Kondo, Momoko Nakamura, Kazuhisa Chonabayashi, Momoko Nishikori, Masakatsu Hishizawa, Kouhei Yamashita, Akifumi Takaori-Kondo
ANNALS OF HEMATOLOGY
(2020)
Correction
Cell & Tissue Engineering
Tadashi Takaki, Azusa Inagaki, Kazuhisa Chonabayashi, Keiji Inoue, Kenji Miki, Seiko Ohno, Takeru Makiyama, Minoru Horie, Yoshinori Yoshida
STEM CELLS INTERNATIONAL
(2020)
Article
Hematology
Hiroki Mizumaki, Kazuyoshi Hosomichi, Kohei Hosokawa, Takeshi Yoroidaka, Tatsuya Imi, Yoshitaka Zaimoku, Takamasa Katagiri, Mai Anh Thi Nguyen, Dung Cao Tran, Mahmoud Ibrahim Yousef Elbadry, Kazuhisa Chonabayashi, Yoshinori Yoshida, Hiroyuki Takamatsu, Tatsuhiko Ozawa, Fumihiro Azuma, Hiroyuki Kishi, Yoichi Fujii, Seishi Ogawa, Atsushi Tajima, Shinji Nakao
Summary: The research found that leukocytes lacking HLA alleles are common in patients with acquired aplastic anemia, possibly due to loss-of-function mutations in certain HLA-A or B alleles. A droplet digital polymerase chain reaction assay was able to accurately detect this mutation, which could serve as a powerful tool for diagnosing the immune pathophysiology of patients with bone marrow failure.
Article
Cell Biology
Sayako Hirose, Takeru Makiyama, Dario Melgari, Yuta Yamamoto, Yimin Wuriyanghai, Fumika Yokoi, Suguru Nishiuchi, Takeshi Harita, Mamoru Hayano, Hirohiko Kohjitani, Jingshan Gao, Asami Kashiwa, Misato Nishikawa, Jie Wu, Jun Yoshimoto, Kazuhisa Chonabayashi, Seiko Ohno, Yoshinori Yoshida, Minoru Horie, Takeshi Kimura
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Oncology
Mikoto Tanabe, Kohei Hosokawa, Mai Anh Thi Nguyen, Noriharu Nakagawa, Kana Maruyama, Noriaki Tsuji, Ryota Urushihara, Luis Espinoza, Mahmoud Elbadry, Md Mohiuddin, Takamasa Katagiri, Masanori Ono, Hiroshi Fujiwara, Kazuhisa Chonabayashi, Yoshinori Yoshida, Hirohito Yamazaki, Atsushi Hirao, Shinji Nakao
Summary: CD109 suppresses TGF-beta signaling in HSPCs, and the lack of CD109 may increase the sensitivity of PIGA-mutated HSPCs to TGF-beta, leading to the preferential commitment of erythroid progenitor cells to mature red blood cells in immune-mediated BM failure.
Article
Hematology
Yuki Morimoto, Kazuhisa Chonabayashi, Hiroshi Kawabata, Chikako Okubo, Makiko Yamasaki-Morita, Misato Nishikawa, Megumi Narita, Azusa Inagaki, Kayoko Nakanishi, Miki Nagao, Akifumi Takaori-Kondo, Yoshinori Yoshida
Summary: This study successfully generated wild-type and mutant ALAS2-induced pluripotent stem cell (iPSC) lines from the peripheral blood cells of a family with pyridoxine-resistant X-linked sideroblastic anemia (XLSA) caused by a missense mutation in the ALAS2 gene. The mutant iPSC lines showed impaired erythroid differentiation potential, immature morphological phenotype, and dysplasia in erythroblasts. Treatment with delta-aminolevainic acid improved the erythroid differentiation ability and hemoglobin expression of mutant iPSC-derived hematopoietic progenitor cells (HPCs). Furthermore, the DNA demethylating agent azacitidine reactivated the silent wild-type ALAS2 allele and improved erythroid differentiation defects in mutant HPCs.
Article
Cell & Tissue Engineering
Yuta Tsujisaka, Takeshi Hatani, Chikako Okubo, Ryo Ito, Azuma Kimura, Megumi Narita, Kazuhisa Chonabayashi, Shunsuke Funakoshi, Antonio Lucena-Cacace, Taro Toyoda, Kenji Osafune, Takeshi Kimura, Hirohide Saito, Yoshinori Yoshida
Summary: By combining miR-switch with MACS technology, we have developed a rapid and efficient method for purifying large amounts of PSC-derived cells. This method can detect specific miRNAs in target cells and use CD4 transgene as a selection marker for MACS, achieving cell purification.
Article
Cardiac & Cardiovascular Systems
Asami Kashiwa, Takeru Makiyama, Hirohiko Kohjitani, Thomas L. Maurissen, Taisuke Ishikawa, Yuta Yamamoto, Yimin Wuriyanghai, Jingshan Gao, Hai Huang, Tomohiko Imamura, Takanori Aizawa, Misato Nishikawa, Kazuhisa Chonabayashi, Hiroyuki Mishima, Seiko Ohno, Futoshi Toyoda, Seiichi Sato, Koh-Ichiro Yoshiura, Kazuhiro Takahashi, Yoshinori Yoshida, Knut Woltjen, Minoru Horie, Naomasa Makita, Takeshi Kimura
Summary: This study investigated the electrophysiological features and pathophysiological mechanisms of the CACNA1C-E1115K mutation using patient-specific induced pluripotent stem cell-derived cardiomyocytes. The mutant cells exhibited impaired calcium current, changes in action potential, and the occurrence of arrhythmia. Evaluation of drug treatment showed that specific antiarrhythmic drugs could shorten the action potential of the mutant cells by modulating the upregulation of sodium current (I-NaL). In conclusion, this study revealed the role of the CACNA1C-E1115K mutation in arrhythmia and provided insights into its underlying mechanisms.
Correction
Hematology
J. Luis Espinoza, Mahmoud I. Elbadry, Kazuhisa Chonabayashi, Yoshinori Yoshida, Takamasa Katagiri, Kenichi Harada, Noriharu Nakagawa, Yoshitaka Zaimoku, Tatsuya Imi, Hiroyuki Takamatsu, Tatsuhiko Ozawa, Hiroyuki Maruyama, Hassan A. Hassanein, Amal Khalifa A. Noreldin, Katsuto Takenaka, Koichi Akashi, Hiroshi Hamana, Hiroyuki Kishi, Yoshiki Akatsuka, Shinji Nakao
Article
Hematology
J. Luis Espinoza, Mahmoud I. Elbadry, Kazuhisa Chonabayashi, Yoshinori Yoshida, Takamasa Katagiri, Kenichi Harada, Noriharu Nakagawa, Yoshitaka Zaimoku, Tatsuya Imi, Hassan A. Hassanein, Amal Khalifa A. Noreldin, Katsuto Takenaka, Koichi Akashi, Hiroshi Hamana, Hiroyuki Kishi, Yoshiki Akatsuka, Shinji Nakao
Article
Cardiac & Cardiovascular Systems
Mamoru Hayano, Takeru Makiyama, Tsukasa Kamakura, Hiroshi Watanabe, Kenichi Sasaki, Shunsuke Funakoshi, Yimin Wuriyanghai, Suguru Nishiuchi, Takeshi Harita, Yuta Yamamoto, Hirohiko Kohjitani, Sayako Hirose, Fumika Yokoi, Jiarong Chen, Osamu Baba, Takahiro Horie, Kazuhisa Chonabayashi, Seiko Ohno, Futoshi Toyoda, Yoshinori Yoshida, Koh Ono, Minoru Horie, Takeshi Kimura
CIRCULATION JOURNAL
(2017)
