Article
Physiology
Kaice A. LaFavers, Chadi A. Hage, Varun Gaur, Radmila Micanovic, Takashi Hato, Shehnaz Khan, Seth Winfree, Simit Doshi, Ranjani N. Moorthi, Homer Twigg, Xue-Ru Wu, Pierre C. Dagher, Edward F. Srour, Tarek M. El-Achkar
Summary: The kidney modulates the immune response in sepsis by enhancing mononuclear phagocyte function through the release of Tamm-Horsfall protein, suggesting potential therapeutic applications in sepsis.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2022)
Article
Chemistry, Analytical
Tianhai Lin, Zhuo Chen, Mengqi Luo, Yang Zhao, Wenjuan Zeng, Shanshan Zheng, Tao Su, Yi Zhong, Shisheng Wang, Youmei Jin, Liqiang Hu, Wanjun Zhao, Jiaxu Li, Xuanyi Wang, Changwei Wu, Dapeng Li, Fang Liu, Guisen Li, Hao Yang, Yong Zhang
Summary: This study developed a simple and fast method for isolating Umod and analyzed its N-glycosylation using mass spectrometry. A total of 780 unique intact N-glycopeptides were identified in healthy individuals, revealing alterations in N-glycosylation of Umod in IgA nephropathy patients. This research provides a high-throughput workflow for understanding the roles of Umod in physiology and pathology, and as a potential diagnostic tool for renal tubular function evolution.
Article
Multidisciplinary Sciences
Kunjing Gong, Min Xia, Yaqin Wang, Na Wang, Ying Liu, Victor Wei Zhang, Hong Cheng, Yuqing Chen
Summary: Genes UMOD, HNF1B, MUC1, REN, and SEC61A1 are associated with autosomal dominant tubulointerstitial kidney disease (ADTKD). A study of 48 probands and their family members revealed potential associations between genotype and outcomes, with UMOD gene cysteine substitutions showing a better prognosis. Follow-up data suggested suspected ADTKD individuals had a better prognosis compared to ADTKD individuals.
SCIENTIFIC REPORTS
(2021)
Review
Medicine, General & Internal
Raisa Thielemans, Reinhart Speeckaert, Charlotte Delrue, Sander De Bruyne, Matthijs Oyaert, Marijn M. Speeckaert
Summary: Uromodulin, also known as Tamm-Horsfall protein, is the predominant urinary protein in healthy individuals. It has been found to be associated with various parameters such as kidney function, graft survival, cardiovascular disease, glucose metabolism, and overall mortality. Reduced levels of urinary uromodulin have been linked to an increased risk of acute kidney injury (AKI) and decreased levels of urinary uromodulin are strongly correlated with variations in estimated glomerular filtration rate in chronic kidney disease (CKD). Serum levels of uromodulin, due to leakage in the thick ascending limb, are closely associated with kidney function and disease severity. The UMOD gene has been identified as being linked to kidney function parameters, CKD risk, hypertension, and kidney stones. Uromodulin holds great promise as a biomarker for understanding kidney function and disease progression.
Review
Urology & Nephrology
Kaice A. LaFavers, Tarek M. El-Achkar
Summary: Tamm-Horsfall protein (THP) is produced by the kidney and released into the urine and circulation. It plays a role in preventing urinary tract infections, kidney stone formation, and regulating ion channels in the kidney. THP knockout mouse models have allowed the investigation of its role in kidney injury and sepsis. Recent studies suggest that THP protects against ischemic injury through inhibition of oxidative stress via TRPM2 channel and promotes macrophage function in sepsis.
SEMINARS IN NEPHROLOGY
(2022)
Article
Urology & Nephrology
Christina B. Joseph, Marta Mariniello, Ayumi Yoshifuji, Guglielmo Schiano, Jennifer Lake, Jonathan Marten, Anne Richmond, Jennifer E. Huffman, Archie Campbell, Sarah E. Harris, Stephan Troyanov, Massimiliano Cocca, Antonietta Robino, Sebastien Theriault, Kai-Uwe Eckardt, Matthias Wuttke, Yurong Cheng, Tanguy Corre, Ivana Kolcic, Corrinda Black, Vanessa Bruat, Maria Pina Concas, Cinzia Sala, Stefanie Aeschbacher, Franz Schaefer, Sven Bergmann, Harry Campbell, Matthias Olden, Ozren Polasek, David J. Porteous, Ian J. Deary, Francois Madore, Philip Awadalla, Giorgia Girotto, Sheila Ulivi, David Conen, Elke Wuehl, Eric Olinger, James F. Wilson, Murielle Bochud, Anna Koettgen, Caroline Hayward, Olivier Devuyst
Summary: This study identifies novel gene loci, KRT40 and WDR72, associated with urinary uromodulin. Functional studies show that KRT40 affects the processing and excretion of uromodulin in kidney cells. This research provides new insights into the biology of uromodulin, keratins, and the UMOD-PDILT locus's influence on kidney function.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2022)
Article
Medicine, General & Internal
Stephan Kemmner, Christopher Holzmann-Littig, Helene Sandberger, Quirin Bachmann, Flora Haberfellner, Carlos Torrez, Christoph Schmaderer, Uwe Heemann, Lutz Renders, Volker Assfalg, Tarek M. El-Achkar, Pranav S. Garimella, Juergen Scherberich, Dominik Steubl
Summary: Higher pretransplant serum uromodulin (sUMOD) levels were independently associated with lower odds of delayed graft function (DGF) following kidney transplantation, potentially serving as a non-invasive marker for stratifying patients based on their risk for developing DGF early on.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Urology & Nephrology
Jung Hyun Shin, Bosik Kang, Myung-Soo Choo
Summary: The study found that the features of HLs in IC patients were not associated with subjective symptoms, but inflammatory changes in HLs were a predictive factor for IC recurrence and associated with frequent urgency episodes and smaller bladder capacity.
JOURNAL OF UROLOGY
(2021)
Review
Spectroscopy
Milani W. Patabandige, Leah D. Pfeifer, Hanna T. Nguyen, Heather Desaire
Summary: The complexity of glycosylation complicates analysis and quantitation, with different quantitative glycomics analysis methods having their own strengths and weaknesses. Considering various quality indicators such as throughput and repeatability is essential for selecting the appropriate method to analyze glycosylation changes.
MASS SPECTROMETRY REVIEWS
(2022)
Article
Plant Sciences
Melanie Deipenbrock, Francesca Scotti, Boris Mo, Michael Heinrich, Andreas Hensel
Summary: Orthosiphon stamineus extract has antiadhesive and anti-infective effects against uropathogenic Escherichia coli, reducing bladder and kidney damage. A clinical trial with 20 healthy volunteers showed that the intake of Java tea resulted in urine samples that significantly inhibited bacterial adhesion and induced gene expression changes in the bacteria, leading to decreased adhesive capacity.
Article
Biochemistry & Molecular Biology
Yuan-Hong Jiang, Jia-Fong Jhang, Lori A. A. Birder, Hann-Chorng Kuo
Summary: The aim of this study was to investigate the expression levels of sensory receptors, inflammatory proteins, and pro-apoptotic proteins in the urothelium of non-Hunner's interstitial cystitis (NHIC) bladders of patients with different clinical and cystoscopic phenotypes. The study concluded that IC/BPS patients with frequency and bladder pain complaints have higher levels of urothelial sensory receptors, and inflammatory and pro-apoptotic proteins. The expression levels of these sensory receptors, inflammatory proteins, and pro-apoptotic proteins are not significantly different among IC/BPS bladders with different conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Urology & Nephrology
Tamehito Onoe, Satoshi Hara, Kazunori Yamada, Takeshi Zoshima, Ichiro Mizushima, Kiyoaki Ito, Takayasu Mori, Shoichiro Daimon, Hiroaki Muramoto, Maki Shimizu, Akira Iguchi, Akihiro Kuma, Yoshifumi Ubara, Michihiro Mitobe, Hiroaki Tsuruta, Nao Kishimoto, Junko Imura, Tadashi Konoshita, Mitsuhiro Kawano
Summary: Autosomal dominant tubulointerstitial kidney disease (ADTKD) is a rare hereditary disease caused by genetic mutations, with carriers at risk of reaching end-stage kidney disease. Kidney biopsy in uromodulin related ADTKD (ADTKD-UMOD) patients showed massive accumulation of UMOD proteins detectable by both immunostaining and conventional methods. These findings provide important clues for diagnosis and suggest that kidney biopsy in ADTKD-UMOD may be more informative than previously assumed.
Article
Medicine, Research & Experimental
Sheon Mary, Philipp Boder, Giacomo Rossitto, Lesley Graham, Kayley Scott, Arun Flynn, David Kipgen, Delyth Graham, Christian Delles
Summary: The study found that salt-loading reduced urinary UMOD excretion in normotensive and chronic hypertensive animals. Nifedipine treatment lowered blood pressure but did not prevent the salt-induced reduction in UMOD excretion.
Article
Immunology
Sherry G. Mansour, Caroline Liu, Yaqi Jia, Peter P. Reese, Isaac E. Hall, Tarek M. El-Achkar, Kaice A. LaFavers, Wassim Obeid, Avi Z. Rosenberg, Parnaz Daneshpajouhnejad, Mona D. Doshi, Enver Akalin, Jonathan S. Bromberg, Meera N. Harhay, Sumit Mohan, Thangamani Muthukumar, Bernd Schroppel, Pooja Singh, Joe M. El-Khoury, Francis L. Weng, Heather R. Thiessen-Philbrook, Chirag R. Parikh
Summary: The study evaluated the relationship between urinary donor UMOD and OPN with recipient graft outcomes, finding that a UMOD:OPN ratio <= 3 was protective, reducing the risk of delayed graft function, improving 6-month estimated glomerular filtration rate, and enhancing graft survival.
Article
Multidisciplinary Sciences
Yuan-Ju Lee, Shang-Jen Chang, Hsiu-Ying Lin, En Meng, Jeff S. Chueh, Chi-Shin Tseng
Summary: This follow-up study assessed the long-term effects of pentosan polysulfate sodium (PPS) monotherapy on recurrent urinary tract infections (UTIs). The study found that PPS significantly reduced the recurrence of UTIs and improved the quality of life for women with recurrent UTI during the 2-year follow-up period.
SCIENTIFIC REPORTS
(2022)